启动子对基因时空表达的调控具有关键作用。本研究采用PCR方法克隆了水稻蔗糖转运基因(sucrose transport genes, SUTs)家族的重要成员之一OsSUT3基因的启动子区域片段,并对该片段进行了顺式调控作用元件的分析。通过构建pOsSUT3::GUS...启动子对基因时空表达的调控具有关键作用。本研究采用PCR方法克隆了水稻蔗糖转运基因(sucrose transport genes, SUTs)家族的重要成员之一OsSUT3基因的启动子区域片段,并对该片段进行了顺式调控作用元件的分析。通过构建pOsSUT3::GUS的表达载体并转入水稻,检测了转基因植株中GUS基因在不同器官的表达,分析了此启动子的表达调控模式。结果显示,此启动子包含着涉及基因表达的多种调控顺式元件,能驱动GUS基因在水稻不同的组织中(叶片,花)中表达。此结果说明,本研究所克隆的OsSUT3基因上游1 628 bp的DNA片段具有启动子活性,能够驱动报告基因的有效表达,可作为在离体条件下研究水稻基因表达和调控作物品种改良中探索目的基因表达的新工具。展开更多
In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improv...In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants （Solanum tuberosum L. cv. D^siree） were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber- specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUTSZ transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUTSZ transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.展开更多
Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and ...Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering （DAF） and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.展开更多
Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield...Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.展开更多
基金supported by the State Key Basic Research and Development Plan of China,the Innovation Foundation of the Chinese Academy of Science,the Program Strategic Scientific Alliances and the China Exchange Program between China and The Netherlands
文摘In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants （Solanum tuberosum L. cv. D^siree） were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber- specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUTSZ transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUTSZ transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.
基金Supported by the State Key Basic Research and Development Plan of China (2004CB720406)Innovation Foundation of the Chinese Academy of Sciences (KSCXZ-SW-306 and KSCX-SW-03)+1 种基金Program Strategic Scientific Alliances (KNAW-PSA, 04-PSA-BD-04 for P.B.F.O.)the China Exchange Program (KNAW-CEP, 04CDp022 for Y.X.) between China and The Netherlands.
文摘Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering （DAF） and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFF1000500)the National Natural Science Foundation of China(Grant Nos.32372099,32172080,and 32188102)the Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-CSCB-202402).
文摘Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.
