目的研究TNF-α、IFN-γ对正常人颊黏膜成纤维细胞(NM-FB)和口腔黏膜下纤维化(OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响.方法取6例OSF患者及5例正常人颊黏膜组织,采用组织块法进行成纤维细胞原代培养并传代,用羟脯氨酸试剂盒观...目的研究TNF-α、IFN-γ对正常人颊黏膜成纤维细胞(NM-FB)和口腔黏膜下纤维化(OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响.方法取6例OSF患者及5例正常人颊黏膜组织,采用组织块法进行成纤维细胞原代培养并传代,用羟脯氨酸试剂盒观察不同浓度TNF-α及IFN-γ对NM-FB和OSF-FB胶原合成的影响.结果浓度为100～10000 U/mL的TNF-α能明显促进NM-FB和OSF-FB的胶原合成(P＜0.05),浓度为40～4000U/mL r IFN-γ则明显抑制两者的胶原合成(P＜0.05),且400U/mL的IFN-γ其抑制率接近峰值(与4 000U/mL的IFN-γ组比,P＞0.05).结论TNF-α促进NM-FB和OSF-FB的胶原合成,而IFN-γ对两者的胶原合成具有抑制作用.展开更多
This study evaluates the effects of a fall experience caused by tripping during the repetitive stepping movements over an obstacle [obstacle-single leg forward step (OSFS) test]. The study included 147 participants wh...This study evaluates the effects of a fall experience caused by tripping during the repetitive stepping movements over an obstacle [obstacle-single leg forward step (OSFS) test]. The study included 147 participants who were divided into 2 groups: 25 fallers caused by tripping and 122 nonfallers. The subjects were asked to step forward over a 10-cm-high obstacle with 1 leg and then return to their original position, as quickly as possible, and this test was repeated for 5 times. The OSFS test was evaluated in 2 phases: the OSFS-F phase, wherein the participants stepped forward on one leg, and the OSFS-R phase, wherein they returned to their original position. Significant differences were observed in both phases of the OSFS test between the two groups, and the fallers by tripping were significantly inferior to the nonfallers. The area under the curve [AUC;area under the receiver operating characteristic (ROC) curve] was more than 0.63 for all the parameters, which was statistically significant. In conclusion, the fallers by tripping were inferior to the nonfallers in the obstacle step movement.展开更多
文摘目的研究TNF-α、IFN-γ对正常人颊黏膜成纤维细胞(NM-FB)和口腔黏膜下纤维化(OSF)患者颊黏膜成纤维细胞(OSF-FB)胶原合成的影响.方法取6例OSF患者及5例正常人颊黏膜组织,采用组织块法进行成纤维细胞原代培养并传代,用羟脯氨酸试剂盒观察不同浓度TNF-α及IFN-γ对NM-FB和OSF-FB胶原合成的影响.结果浓度为100～10000 U/mL的TNF-α能明显促进NM-FB和OSF-FB的胶原合成(P＜0.05),浓度为40～4000U/mL r IFN-γ则明显抑制两者的胶原合成(P＜0.05),且400U/mL的IFN-γ其抑制率接近峰值(与4 000U/mL的IFN-γ组比,P＞0.05).结论TNF-α促进NM-FB和OSF-FB的胶原合成,而IFN-γ对两者的胶原合成具有抑制作用.
文摘This study evaluates the effects of a fall experience caused by tripping during the repetitive stepping movements over an obstacle [obstacle-single leg forward step (OSFS) test]. The study included 147 participants who were divided into 2 groups: 25 fallers caused by tripping and 122 nonfallers. The subjects were asked to step forward over a 10-cm-high obstacle with 1 leg and then return to their original position, as quickly as possible, and this test was repeated for 5 times. The OSFS test was evaluated in 2 phases: the OSFS-F phase, wherein the participants stepped forward on one leg, and the OSFS-R phase, wherein they returned to their original position. Significant differences were observed in both phases of the OSFS test between the two groups, and the fallers by tripping were significantly inferior to the nonfallers. The area under the curve [AUC;area under the receiver operating characteristic (ROC) curve] was more than 0.63 for all the parameters, which was statistically significant. In conclusion, the fallers by tripping were inferior to the nonfallers in the obstacle step movement.
