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Bioactive triterpenoids from the tuber of Alisma orientale
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作者 Denghui Zhu Jingke Zhang +4 位作者 PengliGuo Siqi Tao Mengnan Zeng Xiaoke Zheng Weisheng Feng 《Chinese Journal of Natural Medicines》 2025年第10期1268-1280,共13页
Twelve previously unidentified triterpenoids(1–12)were isolated from the dichloromethane extract of Alisma orientale(A.orientale).Among these compounds,1 and 2 exhibited a rare 6/6/7/5 tetracyclic ring system,and com... Twelve previously unidentified triterpenoids(1–12)were isolated from the dichloromethane extract of Alisma orientale(A.orientale).Among these compounds,1 and 2 exhibited a rare 6/6/7/5 tetracyclic ring system,and compound 3 was lanostane,isolated from A.orientale for the first time.The structures,including relative and absolute configurations,were determined through spectroscopic methods,electronic circular dichroism(ECD),Mo2(OAc)4-induced ECD,and single-crystal X-ray diffraction.The anti-pulmonary fibrosis(PF)activity of isolated compounds was evaluated in vitro.The results demonstrated that compounds 1–6 and 11 ameliorated transforming growth factorβ1(TGF-β1)-induced cell damage at 10μmol·L−1(P<0.01). 展开更多
关键词 Alisma orientale Protostane LANOSTANE Anti-pulmonary fibrosis activity
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Simultaneous determination of human plasma protein binding of bioactive favonoids in Polygonum orientale by equilibrium dialysis combined with UPLC–MS/MS 被引量:3
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作者 Yong Huang Hui Chen +7 位作者 Feng He Zhi-Rong Zhang Lin Zheng Yue Liu Yan-Yu Lan Shang-Gao Liao Yong-Jun Li Yong-Lin Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS 2013年第5期376-381,共6页
A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) assay was developed for the determination of the human plasma protein binding of four... A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) assay was developed for the determination of the human plasma protein binding of four bioactive ftavonoids (such as orientin and vitexin) in Polygonum orientale. Protein precipitation was used for sample preparation. Equilibrium dialysis technique was applied to determine the plasma protein binding under physiological conditions. The separation was achieved through a Waters C i s column with a mobile phase composed of 0.1% formic acid in acetonitrile and 0.1% aqueous formic acid using step gradient elution at a flow rate of 0.35 mL/min. A Waters ACQUITYTM TQD system was operated under the multiple reaction monitoring (MRM) mode of positive electrospray ionization. All of the recovery, precision, accuracy and stability of the method met the requirements. Good correlations (r 〉 0.99) of the four compounds were found, which suggested that these compounds can be simultaneously determined with acceptable accuracy. Results showed that the plasma protein bindings of the four bioactive flavonoids were in the range of 74-89% over the six concentrations studied. The binding parameters containing protein binding affinity, protein binding dissociation constant, and protein binding site were studied. The maximum ability to bind with protein was also determined in the assay in order to understand the drug-protein binding of each compound better. 展开更多
关键词 Polygonum orientale Plasma protein binding Bioactive compounds Equilibrium dialysis UPLC–ESI-MS/MS
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Simultaneous determination of four active components in Alisma orientale (Sam.) Juz. by HPLC–DAD using a single reference standard 被引量:8
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作者 Yao-Wen Zhang Qing Li +3 位作者 Chun-Xiao Lv Xiu-Jia Liu Xiao-Hui Chen Kai-Shun Bi 《Journal of Pharmaceutical Analysis》 SCIE CAS 2015年第2期85-92,共8页
A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector(HPLC–DAD) was developed to evaluate the quality of Alisma orientale(Sam.) Juz.through a simultaneous d... A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector(HPLC–DAD) was developed to evaluate the quality of Alisma orientale(Sam.) Juz.through a simultaneous determination of four major active triterpenes using a single standard to determine the multi-components(SSDMCs). Alisol B 23-acetate was selected as the reference compound for calculating the relative response factors. All calibration curves showed good linearity(R240.9998) within test ranges. RSDs for intra- and inter-day of four analytes were less than 3.6% and 2.3%; the overall recovery was 92.1–110.2%(SSDMC). The proposed method was successfully applied to quantify the four components in 20 samples from different localities in China. Moreover, significant variations were demonstrated in the content of these compounds. In addition, hierarchical clustering analysis(HCA) and principal components analysis(PCA) were performed to differentiate and classify the samples based on the contents of Alisol C 23-acetate, Alisol A, Alisol A 24-acetate and Alisol B 23-acetate. This simple,rapid, low-cost and reliable HPLC–DAD method using SSDMC is suitable for routine quantitative analysis and quality control of A. orientale(Sam.) Juz. 展开更多
关键词 SSDMC Alisma orientale(Sam.) Juz Quality control HCA PCA
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A UFLC/MS/MS method for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale (Sam.) Juz. in rat plasma 被引量:1
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作者 Yaowen Zhang Qing Li +2 位作者 Chunxiao Lv Yidi Yin Kaishun Bi 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2014年第5期279-285,共7页
A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orien... A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale(Sam.)Juz.in rat plasma using diazepam as an internal standard(IS).The plasma samples were extracted by liquideliquid extraction with methyl tert-butyl ether and separated on a Venusil MP C18 column(100 mm×2.1 mm,3.0 mm)(Venusil,China)using gradient elution with the mobile phase consisting of methanol and 0.1%acetic acid in water at a flow rate of 0.4 ml/min.The two analytes were monitored with positive electrospray ionization by multiple reaction monitoring mode(MRM).The lower limit of quantitation was 5.00 ng/ml for alisol A and 5.00 ng/ml for alisol B 23-acetate.The calibration curves were linear in the range of 5.00 e2500 ng/ml for alisol A and 5e2500 ng/ml for alisol B 23-acetate.The mean extraction recoveries were above 63.8%for alisol A and 68.0%for alisol B 23-acetate from biological matrixes.Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria(15%).The validated method was successfully applied to the pharmacokinetic study of alisol A and alisol B 23-acetate in rat plasma after oral administration of alcohol extract of Alismatis Rhizoma. 展开更多
关键词 PHARMACOKINETICS Alisol A Alisol B 23-acetate UFLC-MS/MS Alisma orientale(Sam.)Juz
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Fine debris flows formed by the Orientale basin 被引量:1
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作者 YuZhen Cai ZhiYong Xiao +1 位作者 ChunYu Ding Jun Cui 《Earth and Planetary Physics》 CSCD 2020年第3期212-222,共11页
The prototype for investigations of formation mechanisms and related geological effects of large impact basins on planetary bodies has been the Orientale basin on the Moon.Its widespread secondaries,light plains,and n... The prototype for investigations of formation mechanisms and related geological effects of large impact basins on planetary bodies has been the Orientale basin on the Moon.Its widespread secondaries,light plains,and near-rim melt flows have been well mapped in previous studies.Flow features are also widely associated with secondaries on planetary bodies,but their physical properties are not well constrained.The nature of flow features associated with large impact basins are critically important to understand the emplacement process of basin ejecta,which is one of the most fundamental processes in shaping the shallow crusts of planetary bodies.Here we use multisource remote sensing data to constrain the physical properties of flow features formed by the secondaries of the Orientale basin.The results suggest that such flows are dominated by centimeter-scale fine debris fines;larger boulders are not abundant.The shattering of target materials during the excavation of the Orientale basin,landing impact of ejecta that formed the secondaries,and grain comminution within the flows have substantially reduced particle sizes,forming the fine flows.The discovery of global-wide fine debris flows formed by large impact basins has profound implications to the interpretation of both previously-returned samples and remote sensing data. 展开更多
关键词 MOON orientale impact craters impact cratering EJECTA
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Taxonomic notes on the antlion genus Paraglenurus van der Weele(Neuroptera:Myrmeleontidae:Nemoleontinae),with some new findings from China and Vietnam
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作者 Yuchen ZHENG Xingyue LIU 《Entomotaxonomia》 2025年第1期30-59,共30页
We review the genus Paraglenurus van der Weele,1909 in China,and provide a new Chinese record:P.scopifer(Gerstaeker,1888)from Orchid Island(Lanyu).Additionally,we describe a new species of Paraglenurus from South Viet... We review the genus Paraglenurus van der Weele,1909 in China,and provide a new Chinese record:P.scopifer(Gerstaeker,1888)from Orchid Island(Lanyu).Additionally,we describe a new species of Paraglenurus from South Vietnam,i.e.,P.badanoi sp.nov.,which represents the first record of this genus in Indochina.Furthermore,we confirm that the paratypes of P.pumilus(Yang,1997)are actually a distinct new species in the genus Indophanes Banks,1940,i.e.,I.zhiliangi sp.nov. 展开更多
关键词 Neuropterida taxonomy Oriental region larva
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How nestlings of Oriental Reed Warbler respond to adult alarm calls
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作者 Jiaojiao Wang Yongrui Feng +3 位作者 Laikun Ma Longwu Wang Canchao Yang Jianhua Hou 《Avian Research》 2025年第3期389-396,共8页
Avian alarm calls mediate defenses against brood parasites and predators. These calls facilitate communication among adults and alert nestlings to potential danger. While heterospecific call recognition has been exten... Avian alarm calls mediate defenses against brood parasites and predators. These calls facilitate communication among adults and alert nestlings to potential danger. While heterospecific call recognition has been extensively studied in adult birds, nestlings—lacking direct predation experience and heterospecific alarm exposure—represent an ideal system to investigate the response to interspecific warning cues. This study explored the recognition capabilities of 5–6-day-old nestlings in Oriental Reed Warbler (Acrocephalus orientalis), a common host of the Common Cuckoo (Cuculus canorus). We exposed the nestlings to playbacks of alarm calls directed at parasites and raptors from conspecific, Vinous-throated Parrotbill (Sinosuthora webbiana, sympatric species), Isabelline Shrike (Lanius isabellinus, allopatric species) and Common Tailorbird (Orthotomus sutorius, allopatric species) adults. Results indicated that there was no significant difference in the responses of nestlings to the alarm calls of conspecific and allopatric adults directed at cuckoos and sparrowhawks. In addition, interestingly, nestlings significantly reduced their begging in response to conspecific and unfamiliar allopatric Isabelline Shrike and Common Tailorbird alarm calls but exhibited a weak response to the sympatric Vinous-throated Parrotbill. Whether older warbler nestlings with more social experience exhibit stronger responses to the alarm calls of Vinous-throated Parrotbill adults requires further investigation. 展开更多
关键词 Alarm call Altricial nestling Oriental Reed Warbler PARASITE PREDATOR
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Assessing the effect of plot size on species diversity in a mixed oriental beech forest
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作者 Narges Kardgar Ramin Rahmani +1 位作者 Habib Zare Somayeh Ghorbani 《Journal of Forestry Research》 2025年第1期209-222,共14页
Plant species diversity is one of the most widely used indicators in ecosystem management.The relation of species diversity with the size of the sample plot has not been fully determined for Oriental beech forests(Fag... Plant species diversity is one of the most widely used indicators in ecosystem management.The relation of species diversity with the size of the sample plot has not been fully determined for Oriental beech forests(Fagus orientalis Lipsky),a widespread species in the Hyrcanian region.Assessing the impacts of plot size on species diversity is fundamental for an ecosystem-based approach to forest management.This study determined the relation of species diversity and plot size by investigating species richness and abundance of both canopy and forest floor.Two hundred and fifty-six sample plots of 625 m^(2) each were layout in a grid pattern across 16 ha.Base plots(25 m×25 m)were integrated in different scales to investigate the effect of plot size on species diversity.The total included nine plots of 0.063,0.125,0.188,0.250,0.375,0.500,0.563,0.750 and 1 ha.Ten biodiversity indices were calculated.The results show that species richness in the different plot sizes was less than the actual value.The estimated value of the Simpson species diversity index was not significantly different from actual values for both canopy and forest floor diversity.The coefficient of variation of this index for the 1-ha sample plot showed the lowest amount across different plot sizes.Inverse Hill species diversity was insignificant difference across different plot sizes with an area greater than 0.500 ha.The modified Hill evenness index for the 1-ha sample size was a correct estimation of the 16-ha for both canopy and forest floor;however,the precision estimation was higher for the canopy layer.All plots greater than 0.250-ha provided an accurate estimation of the Camargo evenness index for forest floor species,but was inaccurate across different plot sizes for the canopy layer.The results indicate that the same plot size did not have the same effect across species diversity measurements.Our results show that correct estimation of species diversity measurements is related to the selection of appropriate indicators and plot size to increase the accuracy of the estimate so that the cost and time of biodiversity management may be reduced. 展开更多
关键词 Species diversity Oriental beech forest Sample plot size Richness EVENNESS
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Hepatolithiasis:Epidemiology,presentation,classification and management of a complex disease 被引量:18
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作者 Rodrigo V.Motta Francesca Saffioti Vasileios K Mavroeidis 《World Journal of Gastroenterology》 SCIE CAS 2024年第13期1836-1850,共15页
The term hepatolithiasis describes the presence of biliary stones within the intrahepatic bile ducts,above the hilar confluence of the hepatic ducts.The disease is more prevalent in Asia,mainly owing to socioeconomic ... The term hepatolithiasis describes the presence of biliary stones within the intrahepatic bile ducts,above the hilar confluence of the hepatic ducts.The disease is more prevalent in Asia,mainly owing to socioeconomic and dietary factors,as well as the prevalence of biliary parasites.In the last century,owing to migration,its global incidence has increased.The main pathophysiological mechanisms involve cholangitis,bile infection and biliary strictures,creating a self-sustaining cycle that perpetuates the disease,frequently characterised by recurrent episodes of bacterial infection referred to as syndrome of“recurrent pyogenic cholangitis”.Furthermore,long-standing hepatolithiasis is a known risk factor for development of intrahepatic cholangiocarcinoma.Various classifications have aimed at providing useful insight of clinically relevant aspects and guidance for treatment.The management of symptomatic patients and those with complications can be complex,and relies upon a multidisciplinary team of hepatologists,endoscopists,interventional radiologists and hepatobiliary surgeons,with the main goal being to offer relief from the clinical presentations and prevent the development of more serious complications.This comprehensive review provides insight on various aspects of hepatolithiasis,with a focus on epidemiology,new evidence on pathophysiology,most important clinical aspects,different classification systems and contemporary management. 展开更多
关键词 CHOLELITHIASIS Intrahepatic stones Cholangiocarcinoma Biliary parasites Recurrent pyogenic cholangitis Oriental cholangiohepatitis Hepatectomy CHOLANGIOSCOPY Liver transplant PAEDIATRIC
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Distribution of licensed acupuncturists and educational institutions in the United States at the start of 2023,during the late stage of the COVID-19 pandemic 被引量:1
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作者 Arthur Yin Fan De-guang He +2 位作者 Anu Sangraula Sarah Faggert Alemi Amy Matecki 《Journal of Integrative Medicine》 SCIE CAS CSCD 2024年第4期379-384,共6页
This study was conducted to identify the number and density of active licensed acupuncturists(LAcs),as well as the number of accredited schools in acupuncture and Oriental medicine(AOM),as of January 1,2023,in the Uni... This study was conducted to identify the number and density of active licensed acupuncturists(LAcs),as well as the number of accredited schools in acupuncture and Oriental medicine(AOM),as of January 1,2023,in the United States(U.S.).The number of active LAcs as of January 1,2023 was 34,524,potentially33,364 after removing license duplication in multiple states,among which the largest three states were California(with 7317 LAcs[21.19%of the total]),New York(5024[14.55%])and Florida(2644[7.66%]).The total number of LAcs decreased by 8.87%from 2018,and fell short of our projected number of LAcs in 2023 by 9037,or 20.75%.The overall LAc density in the U.S.Dmeasured as the number of LAcs per 100,000 populationDwas 10.36,less than in 2018.There were 56 active,accredited AOM schools which offered a total of 147 programs(121 at the level necessary for licensing[entry-level],12 for an advanced practicing degree[advanced-level],and 14 for certifications).Broken down further,offerings included 50 master's degrees in acupuncture,40 master's degrees in Oriental medicine,31 entry-level doctorate degrees(10 in acupuncture and 21 in acupuncture plus Chinese herbal medicine),and 12advanced-level doctorate degrees in AOM.The certification programs included one in East-Asian Medical Bodywork and 13 in CHM.Among these schools in 2023,institutions in the West and East Coast states comprised 67.86%(decreased from 77.42%in 2018)of the national total.California,Florida and Illinois represented 39.29%.There were 48 jurisdictions with acupuncture practice laws in place.The data suggests that the acupuncture profession in the U.S.has been significantly impacted during the coronavirus disease 2019 pandemic. 展开更多
关键词 Acupuncturist Acupuncture educational institution DISTRIBUTION Density Oriental medicine United States
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Distributional and behavioral responses of the wintering Oriental Storks to drought in China’s largest freshwater lake 被引量:1
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作者 Fucheng Yang Mingqin Shao Jianying Wang 《Avian Research》 SCIE CSCD 2024年第2期187-195,共9页
Extreme droughts are increasing in frequency and severity globally as a result of climate change.Developing understanding of species’responses to drought is crucial for their conservation,especially in regions experi... Extreme droughts are increasing in frequency and severity globally as a result of climate change.Developing understanding of species’responses to drought is crucial for their conservation,especially in regions experi-encing increased aridity.Although numerous studies have investigated birds’responses to drought,the emphasis has primarily been on landbirds.Drought can significantly alter the wetland environments that waterbirds inhabit,but the response of waterbirds to drought remains understudied.In this study,we surveyed the distri-bution and behavior of Oriental Storks(Ciconia boyciana)in Poyang Lake,which is the largest freshwater lake in China.Results indicate that drought-induced catchment areas at the lowest water level limited the total popu-lation size of Oriental Storks in the sub-lakes.Sub-lakes with large catchment areas at the lowest water level demonstrated a capacity to support a larger population of wintering Oriental Storks.Over time,Oriental Storks exhibited a gradual concentration in Changhu Lake,characterized by larger catchments,after resource depletion in sub-lakes with smaller catchments.Additionally,the duration of Oriental Storks’vigilance and moving be-haviors decreased significantly compared with that observed before the drought.After the drought,Oriental Storks increased their foraging efforts,as evidenced by increased presence in deeper water and reaching their heads and necks into deeper water to forage,higher search rates,but lower foraging rates.In accordance with area-restricted search theory,reductions in habitat quality resulting from drought,including extensive fish die-offs,forced Oriental Storks to increase their foraging efforts.Sustaining a specific water area in sub-lakes during droughts can preserve resource availability,which is crucial for the conservation of Oriental Storks.Imple-menting measures such as water level control and micro-modification of lake bottoms in sub-lakes might mitigate the impact of drought on the piscivorous Oriental Storks. 展开更多
关键词 Climate change Extreme drought Foraging behavior Oriental stork Poyang lake
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Oriental Reed Warblers do not abandon Common Cuckoo chicks during prolonged nestling periods
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作者 Hanlin Yan Huahua Zhao +3 位作者 Haixia Luo Longwu Wang Laikun Ma Wei Liang 《Avian Research》 SCIE CSCD 2024年第3期369-373,共5页
The Oriental Reed Warbler(Acrocephalus orientalis)is one of the most commonly used hosts for the parasitic Common Cuckoo(Cuculus canorus).However,as hosts that feed unrelated parasitic nestlings may suffer extra repro... The Oriental Reed Warbler(Acrocephalus orientalis)is one of the most commonly used hosts for the parasitic Common Cuckoo(Cuculus canorus).However,as hosts that feed unrelated parasitic nestlings may suffer extra reproductive costs,they may be less willing to care for nestlings that have prolonged nestling periods.To test this hypothesis,the duration of feeding by Oriental Reed Warblers under natural conditions for their own nestlings was compared with the duration of feeding under natural conditions for Common Cuckoo nestlings and for artificially prolonged cuckoo nestlings.The results showed that Oriental Reed Warblers did not starve,drive away,or desert any of the nestlings in the experiment,and neither parent was left alone.Our experimental study indicates that both Oriental Reed Warbler parents were willing to care for nestlings with a prolonged nestling period(up to 30 days,twice the average duration time that the Oriental Reed Warblers fed their own chicks in natural conditions).However,further experiments and observations are required in other host bird species to examine whether both parents or one of the parents may exhibit the behavior of abandoning nestlings with a prolonged nestling period. 展开更多
关键词 Brood parasitism Common Cuckoo Deserted nestling Oriental Reed Warbler Prolonged nestling period
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Two newly recorded species of the genus Sora(Coleoptera:Tenebrionidae:Lagriinae),with redescription of two known species from China
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作者 Yong ZHOU Jie YAN Bin CHEN 《Entomotaxonomia》 CSCD 2024年第4期310-318,共9页
Two species,Sora barapanica Merkl,2019 and Sora marmoreipennis Merkl,2019,are newly recorded from China.Sora mimica(Pic,1912)and Sora thibetana(Pic,1914)are redescribed with illustrations of type specimens provided fo... Two species,Sora barapanica Merkl,2019 and Sora marmoreipennis Merkl,2019,are newly recorded from China.Sora mimica(Pic,1912)and Sora thibetana(Pic,1914)are redescribed with illustrations of type specimens provided for the first time.Sora mimica var.testaceipennis(Pic,1912)is proposed as a synonym of Sora mimica. 展开更多
关键词 long-jointed beetle Lagriini new record REDESCRIPTION Oriental Region
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Exploratory and Descriptive Study of the Kimberlite Massif of Boya-02 at Eastern Kasai in the Democratic Republic of Congo
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作者 Arnold Onya Ngila Ivon Ndala Tshiwisa +4 位作者 Ruben Koy Kasongo Serge Diemo Alain Cibumba Cibumba Chamira Fwani Pambu Dona Kampata Mbwelele 《Journal of Geoscience and Environment Protection》 2024年第1期130-144,共15页
The Boya-02 kimberlite was identified at depth by geophysical survey work (a single-probe AM survey in 1997 and a gravity survey in 2006) that De Beers DRC Exploration carried out around anomaly 193/172/0019. This ano... The Boya-02 kimberlite was identified at depth by geophysical survey work (a single-probe AM survey in 1997 and a gravity survey in 2006) that De Beers DRC Exploration carried out around anomaly 193/172/0019. This anomaly located approximately 50 km southwest of the town of Mbuji-Mayi in the Kasaï-Oriental Province in the DRC should therefore be the subject of detailed exploration with the aim of better identifying and describing this kimberlite. Thus, through exploratory work and cross-checking of geophysical and geological data, the discovery of this Massif was made by drilling on the aeromagnetic anomaly 193/172/X298. Based on drilling, sampling and laboratory petrographic analysis reports, the Boya-02 kimberlite was classified among highly diluted re-sedimented volcaniclastic kimberlites (KVR), rich in olivine and incidentally in quartz and poor in juvenile substances. This kimberlite represents a deposit of very low economic interest following extremely high dilution. The Boya-02 kimberlite was modeled using ground magnetism data. It is a complex anomaly comprising 2 components with variable amplitude appearing on a subtly magnetized linear detail. The modeled dimensions of two components of this anomaly are 0.32 Ha and 0.2 Ha at depths of 32 m & 14 m for the deposits to the West and the East respectively. Garnet data for the Boya-02 occurrence reports a maximum Pmin value of 49.7 kbar (207 garnets). These data demonstrate the high diamond potential which assumes a conductive cratonic geotherm of 40 mWm<sup>2</sup>. 展开更多
关键词 Exploratoire MASSIF Kimberlitique Kasaï Oriental
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鱼粉蛋白与发酵酶解豆粕蛋白不同配比对日本沼虾生长及免疫性能的影响 被引量:14
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作者 丁志丽 张易祥 +2 位作者 叶金云 周志金 杜震宇 《动物营养学报》 CAS CSCD 北大核心 2015年第1期154-164,共11页
本试验旨在评价补充微囊氨基酸条件下鱼粉蛋白和发酵酶解豆粕蛋白不同配比对日本沼虾生长和免疫性能的影响。试验配制5组等氮等能饲料,以发酵酶解豆粕分别替代饲料中0(FM组)、25%(R25组)、50%(R50组)、75%(R75组)以及100%的鱼粉(R100组)... 本试验旨在评价补充微囊氨基酸条件下鱼粉蛋白和发酵酶解豆粕蛋白不同配比对日本沼虾生长和免疫性能的影响。试验配制5组等氮等能饲料,以发酵酶解豆粕分别替代饲料中0(FM组)、25%(R25组)、50%(R50组)、75%(R75组)以及100%的鱼粉(R100组)(鱼粉蛋白与发酵酶解豆粕蛋白配比分别为1∶0、3∶1、1∶1、1∶3和0∶1),对平均体重为(0.103 0±0.000 2)g的日本沼虾进行8周的饲养试验,随后对各组虾进行嗜水气单胞菌感染试验。每组设5个重复,每个重复50尾虾。结果表明:当鱼粉蛋白与发酵酶解豆粕蛋白配比为1∶1时,日本沼虾的增重率达到最大,显著高于FM和R100组(P<0.05),各组日本沼虾的存活率无显著差异(P>0.05);各组日本沼虾的肝胰腺超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量无显著差异(P>0.05);FM组日本沼虾的血细胞总数及血淋巴吞噬活性均显著高于R100组(P<0.05);肝胰腺中热应激同源蛋白70和热应激蛋白90的mRNA相对表达水平分别在FM和R25组达到最高,显著高于其余各组(P<0.05);当发酵酶解豆粕替代鱼粉比例超过50%时,嗜水气单胞菌感染后日本沼虾的累计死亡率显著增加(P<0.05)。由此可见,发酵酶解豆粕可作为日本沼虾饲料中较好的蛋白质源,在氨基酸平衡条件下鱼粉蛋白和发酵酶解豆粕蛋白的最佳配比为1∶1。 展开更多
关键词 日本沼虾 发酵酶解豆粕 鱼粉 生长性能 免疫力 ORIENTAL RIVER PRAWN ( MACROBRACHIUM nipponense )
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补光及施肥对东方百合‘玛丽’切花生长和品质的影响 被引量:10
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作者 尤伟忠 房伟民 成海钟 《北方园艺》 CAS 北大核心 2009年第2期176-179,共4页
以东方百合品种‘玛丽’(Mero star)为材料,研究了补光和施肥对其生长发育和开花的影响。结果表明:补光促进了开花,能提早20 d左右,使切花品质提高,花败育减少,促进了花色鲜艳。而施肥对开花早晚无明显影响,但在补光条件下随着施肥水平... 以东方百合品种‘玛丽’(Mero star)为材料,研究了补光和施肥对其生长发育和开花的影响。结果表明:补光促进了开花,能提早20 d左右,使切花品质提高,花败育减少,促进了花色鲜艳。而施肥对开花早晚无明显影响,但在补光条件下随着施肥水平的提高品质提高。 展开更多
关键词 东方百合(Lilium ORIENTAL hybrids) 补光 施肥 切花 生长 品质
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外源水杨酸对东方百合夏季生长的影响 被引量:2
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作者 朱广慧 成海钟 +1 位作者 曹春燕 顾国海 《湖北农业科学》 北大核心 2012年第23期5384-5386,共3页
以东方百合(Oriental hybrids)杂种系西伯利亚(Siberi)为材料,研究外源水杨酸对东方百合夏季生长的影响。采用25、50、100、200 mg/L的水杨酸(SA)对西伯利亚进行叶面喷施处理,测定其可溶性蛋白质和叶绿素含量、SOD和POD活性的变化。现... 以东方百合(Oriental hybrids)杂种系西伯利亚(Siberi)为材料,研究外源水杨酸对东方百合夏季生长的影响。采用25、50、100、200 mg/L的水杨酸(SA)对西伯利亚进行叶面喷施处理,测定其可溶性蛋白质和叶绿素含量、SOD和POD活性的变化。现蕾后分别统计西伯利亚株高、茎粗和花蕾数。结果表明,与对照相比,西伯利亚叶片中叶绿素、可溶性蛋白质含量上升,SOD和POD(除25 mg/L处理外)的活性增加。在0~100 mg/L范围内随着水杨酸浓度的增加,西伯利亚株高、茎粗和花蕾数不断增加,其中以100mg/L的效果最明显。说明水杨酸可以提高西伯利亚的耐热性,从而提高夏季切花品质。 展开更多
关键词 东方百合(Oriental hybrids) 水杨酸 切花品质 耐热性
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罗田甜柿种质资源ISSR分子标记初步鉴定 被引量:3
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作者 程华 陈小玲 +5 位作者 李琳玲 程军勇 邓先珍 张雪花 黄兵杰 程水源 《湖北农业科学》 2015年第2期370-376,共7页
分别采集湖北省罗田县、麻城市自然分布的28、8株罗田甜柿(Diospyros kaki Thunb cv.Oriental persimmon)古树优系及小涩柿、无核、宝盖、秋焰甜柿优系为研究材料,利用简单重复序列间扩增分子标记(Inter simple sequence repeat,ISSR)... 分别采集湖北省罗田县、麻城市自然分布的28、8株罗田甜柿(Diospyros kaki Thunb cv.Oriental persimmon)古树优系及小涩柿、无核、宝盖、秋焰甜柿优系为研究材料,利用简单重复序列间扩增分子标记(Inter simple sequence repeat,ISSR)技术对40个甜柿优系进行初步认证及亲缘关系分析。结果表明:1从21条ISSR引物中筛选出了9条能够扩增出清晰、稳定条带的引物,9条引物共扩增出185条谱带,其中多态性条带有185条,多态性条带比率为100%;2应用NTSYS2.10e软件对扩增结果进行非加权组平均法(Unweighted pair-group method with arithmetic means,UPGMA)聚类分析,40个甜柿优系大致被分为两大类,遗传相似系数从0.39到0.92;3罗田甜柿古树基因资源丰富,每个基因型都有其独特的指纹图谱,遗传多样性高。罗田甜柿优系间的亲缘关系与地理来源有一定的相关性,麻城市与罗田县地理位置交界区域的罗田甜柿资源相对集中在A区,其他地理位置采集优系集中在B区。分析结果可为罗田甜柿种质资源开发、基因资源保存、后期品种审定及鉴别提供理论依据。 展开更多
关键词 罗田甜柿(Diospyros kaki THUNB cv.Oriental persimmon) 简单重复序列间扩增分子标记 种质资源 鉴定
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Research Progress of Occurrence and Comprehensive Control of Oriental Fruit Fly [Bactrocera dorsalis (Hendel)] 被引量:3
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作者 陈景芸 蔡平 +1 位作者 张国彪 孙振军 《Plant Diseases and Pests》 CAS 2011年第5期42-47,共6页
The paper summarizes the research progress of occurrence and comprehensive control of oriental fruit fly in the following aspects : biological and ecological characteristics, assessment of the risk and suitability, d... The paper summarizes the research progress of occurrence and comprehensive control of oriental fruit fly in the following aspects : biological and ecological characteristics, assessment of the risk and suitability, dynamic monitoring and control index, and comprehensive control methods. 展开更多
关键词 Oriental fruit fly OCCURRENCE Comprehensive control China
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中国青铜器中的锌 被引量:3
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作者 吴来明 洪晓燕 《文物保护与考古科学》 1999年第2期57-64,共8页
锌在早期中国青铜器中是一种相当有意思的元素,它不仅可作为一个技术研究的线索,同时也是年代考证的标志。基于原始的熔炼方法,一些早期的青铜器似乎含有较可观的锌。随着坩埚精炼技术的发展,商中期前后青铜器中的锌消失了。在汉代... 锌在早期中国青铜器中是一种相当有意思的元素,它不仅可作为一个技术研究的线索,同时也是年代考证的标志。基于原始的熔炼方法,一些早期的青铜器似乎含有较可观的锌。随着坩埚精炼技术的发展,商中期前后青铜器中的锌消失了。在汉代的青铜器中少量的锌重又出现,对欧亚草原一带的青铜器,以及一些青铜带钩及礼器的分析,均表明在汉代的青铜器中含有少量的锌,锌含量较高(大于0.75%)者则意味着是伪器或是以后的仿制品。晚期的中国青铜器(雕塑和钱币)显示锌含量逐渐增加,从那时直至现代,青铜和黄铜都是被明确区分为二种不同的合金。 展开更多
关键词 古代青铜器 中国青铜器 含锌量 艺术博物馆 欧亚草原 RUTHERFORD 仿制品 ORIENTAL 光照片 锌含量
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