Twelve previously unidentified triterpenoids(1–12)were isolated from the dichloromethane extract of Alisma orientale(A.orientale).Among these compounds,1 and 2 exhibited a rare 6/6/7/5 tetracyclic ring system,and com...Twelve previously unidentified triterpenoids(1–12)were isolated from the dichloromethane extract of Alisma orientale(A.orientale).Among these compounds,1 and 2 exhibited a rare 6/6/7/5 tetracyclic ring system,and compound 3 was lanostane,isolated from A.orientale for the first time.The structures,including relative and absolute configurations,were determined through spectroscopic methods,electronic circular dichroism(ECD),Mo2(OAc)4-induced ECD,and single-crystal X-ray diffraction.The anti-pulmonary fibrosis(PF)activity of isolated compounds was evaluated in vitro.The results demonstrated that compounds 1–6 and 11 ameliorated transforming growth factorβ1(TGF-β1)-induced cell damage at 10μmol·L−1(P<0.01).展开更多
A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） assay was developed for the determination of the human plasma protein binding of four...A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） assay was developed for the determination of the human plasma protein binding of four bioactive ftavonoids （such as orientin and vitexin） in Polygonum orientale. Protein precipitation was used for sample preparation. Equilibrium dialysis technique was applied to determine the plasma protein binding under physiological conditions. The separation was achieved through a Waters C i s column with a mobile phase composed of 0.1% formic acid in acetonitrile and 0.1% aqueous formic acid using step gradient elution at a flow rate of 0.35 mL/min. A Waters ACQUITYTM TQD system was operated under the multiple reaction monitoring （MRM） mode of positive electrospray ionization. All of the recovery, precision, accuracy and stability of the method met the requirements. Good correlations （r 〉 0.99） of the four compounds were found, which suggested that these compounds can be simultaneously determined with acceptable accuracy. Results showed that the plasma protein bindings of the four bioactive flavonoids were in the range of 74-89% over the six concentrations studied. The binding parameters containing protein binding affinity, protein binding dissociation constant, and protein binding site were studied. The maximum ability to bind with protein was also determined in the assay in order to understand the drug-protein binding of each compound better.展开更多
A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector（HPLC–DAD） was developed to evaluate the quality of Alisma orientale（Sam.） Juz.through a simultaneous d...A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector（HPLC–DAD） was developed to evaluate the quality of Alisma orientale（Sam.） Juz.through a simultaneous determination of four major active triterpenes using a single standard to determine the multi-components（SSDMCs）. Alisol B 23-acetate was selected as the reference compound for calculating the relative response factors. All calibration curves showed good linearity（R240.9998） within test ranges. RSDs for intra- and inter-day of four analytes were less than 3.6% and 2.3%; the overall recovery was 92.1–110.2%（SSDMC）. The proposed method was successfully applied to quantify the four components in 20 samples from different localities in China. Moreover, significant variations were demonstrated in the content of these compounds. In addition, hierarchical clustering analysis（HCA） and principal components analysis（PCA） were performed to differentiate and classify the samples based on the contents of Alisol C 23-acetate, Alisol A, Alisol A 24-acetate and Alisol B 23-acetate. This simple,rapid, low-cost and reliable HPLC–DAD method using SSDMC is suitable for routine quantitative analysis and quality control of A. orientale（Sam.） Juz.展开更多
A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orien...A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale(Sam.)Juz.in rat plasma using diazepam as an internal standard(IS).The plasma samples were extracted by liquideliquid extraction with methyl tert-butyl ether and separated on a Venusil MP C18 column(100 mm×2.1 mm,3.0 mm)(Venusil,China)using gradient elution with the mobile phase consisting of methanol and 0.1%acetic acid in water at a flow rate of 0.4 ml/min.The two analytes were monitored with positive electrospray ionization by multiple reaction monitoring mode(MRM).The lower limit of quantitation was 5.00 ng/ml for alisol A and 5.00 ng/ml for alisol B 23-acetate.The calibration curves were linear in the range of 5.00 e2500 ng/ml for alisol A and 5e2500 ng/ml for alisol B 23-acetate.The mean extraction recoveries were above 63.8%for alisol A and 68.0%for alisol B 23-acetate from biological matrixes.Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria(15%).The validated method was successfully applied to the pharmacokinetic study of alisol A and alisol B 23-acetate in rat plasma after oral administration of alcohol extract of Alismatis Rhizoma.展开更多
The prototype for investigations of formation mechanisms and related geological effects of large impact basins on planetary bodies has been the Orientale basin on the Moon.Its widespread secondaries,light plains,and n...The prototype for investigations of formation mechanisms and related geological effects of large impact basins on planetary bodies has been the Orientale basin on the Moon.Its widespread secondaries,light plains,and near-rim melt flows have been well mapped in previous studies.Flow features are also widely associated with secondaries on planetary bodies,but their physical properties are not well constrained.The nature of flow features associated with large impact basins are critically important to understand the emplacement process of basin ejecta,which is one of the most fundamental processes in shaping the shallow crusts of planetary bodies.Here we use multisource remote sensing data to constrain the physical properties of flow features formed by the secondaries of the Orientale basin.The results suggest that such flows are dominated by centimeter-scale fine debris fines;larger boulders are not abundant.The shattering of target materials during the excavation of the Orientale basin,landing impact of ejecta that formed the secondaries,and grain comminution within the flows have substantially reduced particle sizes,forming the fine flows.The discovery of global-wide fine debris flows formed by large impact basins has profound implications to the interpretation of both previously-returned samples and remote sensing data.展开更多
We review the genus Paraglenurus van der Weele,1909 in China,and provide a new Chinese record:P.scopifer(Gerstaeker,1888)from Orchid Island(Lanyu).Additionally,we describe a new species of Paraglenurus from South Viet...We review the genus Paraglenurus van der Weele,1909 in China,and provide a new Chinese record:P.scopifer(Gerstaeker,1888)from Orchid Island(Lanyu).Additionally,we describe a new species of Paraglenurus from South Vietnam,i.e.,P.badanoi sp.nov.,which represents the first record of this genus in Indochina.Furthermore,we confirm that the paratypes of P.pumilus(Yang,1997)are actually a distinct new species in the genus Indophanes Banks,1940,i.e.,I.zhiliangi sp.nov.展开更多
Avian alarm calls mediate defenses against brood parasites and predators. These calls facilitate communication among adults and alert nestlings to potential danger. While heterospecific call recognition has been exten...Avian alarm calls mediate defenses against brood parasites and predators. These calls facilitate communication among adults and alert nestlings to potential danger. While heterospecific call recognition has been extensively studied in adult birds, nestlings—lacking direct predation experience and heterospecific alarm exposure—represent an ideal system to investigate the response to interspecific warning cues. This study explored the recognition capabilities of 5–6-day-old nestlings in Oriental Reed Warbler (Acrocephalus orientalis), a common host of the Common Cuckoo (Cuculus canorus). We exposed the nestlings to playbacks of alarm calls directed at parasites and raptors from conspecific, Vinous-throated Parrotbill (Sinosuthora webbiana, sympatric species), Isabelline Shrike (Lanius isabellinus, allopatric species) and Common Tailorbird (Orthotomus sutorius, allopatric species) adults. Results indicated that there was no significant difference in the responses of nestlings to the alarm calls of conspecific and allopatric adults directed at cuckoos and sparrowhawks. In addition, interestingly, nestlings significantly reduced their begging in response to conspecific and unfamiliar allopatric Isabelline Shrike and Common Tailorbird alarm calls but exhibited a weak response to the sympatric Vinous-throated Parrotbill. Whether older warbler nestlings with more social experience exhibit stronger responses to the alarm calls of Vinous-throated Parrotbill adults requires further investigation.展开更多
Plant species diversity is one of the most widely used indicators in ecosystem management.The relation of species diversity with the size of the sample plot has not been fully determined for Oriental beech forests(Fag...Plant species diversity is one of the most widely used indicators in ecosystem management.The relation of species diversity with the size of the sample plot has not been fully determined for Oriental beech forests(Fagus orientalis Lipsky),a widespread species in the Hyrcanian region.Assessing the impacts of plot size on species diversity is fundamental for an ecosystem-based approach to forest management.This study determined the relation of species diversity and plot size by investigating species richness and abundance of both canopy and forest floor.Two hundred and fifty-six sample plots of 625 m^(2) each were layout in a grid pattern across 16 ha.Base plots(25 m×25 m)were integrated in different scales to investigate the effect of plot size on species diversity.The total included nine plots of 0.063,0.125,0.188,0.250,0.375,0.500,0.563,0.750 and 1 ha.Ten biodiversity indices were calculated.The results show that species richness in the different plot sizes was less than the actual value.The estimated value of the Simpson species diversity index was not significantly different from actual values for both canopy and forest floor diversity.The coefficient of variation of this index for the 1-ha sample plot showed the lowest amount across different plot sizes.Inverse Hill species diversity was insignificant difference across different plot sizes with an area greater than 0.500 ha.The modified Hill evenness index for the 1-ha sample size was a correct estimation of the 16-ha for both canopy and forest floor;however,the precision estimation was higher for the canopy layer.All plots greater than 0.250-ha provided an accurate estimation of the Camargo evenness index for forest floor species,but was inaccurate across different plot sizes for the canopy layer.The results indicate that the same plot size did not have the same effect across species diversity measurements.Our results show that correct estimation of species diversity measurements is related to the selection of appropriate indicators and plot size to increase the accuracy of the estimate so that the cost and time of biodiversity management may be reduced.展开更多
The study describes a new species,Syndiamesa fanduangularis Fu,sp.nov.,representing the first record of the genus Syndiamesa from the Oriental Region.A detailed description of the new species is provided,including mor...The study describes a new species,Syndiamesa fanduangularis Fu,sp.nov.,representing the first record of the genus Syndiamesa from the Oriental Region.A detailed description of the new species is provided,including morphological data and illustrations.Molecular data(COI gene)were obtained and deposited in NCBI GenBank,providing a DNA barcode for species identification.The study also provides an updated key to adult males of the genus Syndiamesa globally.展开更多
The paper summarizes the research progress of occurrence and comprehensive control of oriental fruit fly in the following aspects ： biological and ecological characteristics, assessment of the risk and suitability, d...The paper summarizes the research progress of occurrence and comprehensive control of oriental fruit fly in the following aspects ： biological and ecological characteristics, assessment of the risk and suitability, dynamic monitoring and control index, and comprehensive control methods.展开更多
AIM： To examine the effects of traditional Chinese medicine （TCM） on alcohol-induced fatty liver in rats. TCM consists of Astragalus membranaceus, Morus alba, Crataegus pinnatifida, Alisma orientale, Salvia miltior...AIM： To examine the effects of traditional Chinese medicine （TCM） on alcohol-induced fatty liver in rats. TCM consists of Astragalus membranaceus, Morus alba, Crataegus pinnatifida, Alisma orientale, Salvia miltiorrhiza, and Pueraria lobata. METHODS： The rats were separated randomly into five groups. One （the CD group） was fed a control diet for 10 wk, another （the ED group） fed an ethanol-containing isocaloric liquid diet for 10 wk, and the last three （the TCM group） were fed an ethanol-containing isocaloric liquid diet for 10 wk and dosed orally with TCM （222 mg/kg.d, TCM222; 667 mg/kg·d, TCM667; and 2 000 mg/kg.d, TCM2000, respectively） weekly during the last 4 wk. RESULTS： ED group developed fatty liver according to lipid profile and liver histological findings. Compared with the control group, liver/body weight, serum triglyceride （TG） and total cholesterol （TC）, liver TG and TC, serum alanine aminotransferase （ALT） and aspartic aminotransferase （AST） significantly increased in the ED group. Whereas, in the rats administered with TCM, liver/body weight, serum TG and TC, liver TG and TC, serum ALT and AST were significantly decreased, and the degree of hepatic lipid droplets was markedly improved compared with those in the ED group. CONCLUSION： TCM treatment causes significant reduction in alcohol-induced lipid hepatic accumulation, reversing fatty liver and liver damage, and can be used as a remedy for alcoholic fatty liver.展开更多
The artificial nestbox on an Asian White Birch (Betula platyphylla) (1360 m in elevation) was used by a pair of Yellow-rumped Flycatcher (Ficedula zanthopygia) in Beijing, and five eggs were found in the nest in 2005....The artificial nestbox on an Asian White Birch (Betula platyphylla) (1360 m in elevation) was used by a pair of Yellow-rumped Flycatcher (Ficedula zanthopygia) in Beijing, and five eggs were found in the nest in 2005. One was much larger and was identified as the Oriental Cuckoo’s (Cuculus optatus) egg.展开更多
Oripavine is the major alkaloid of Papaver orientale. It is an important intermediate in the biosynthesis of morphine alkaloids. Recently, new Papaver somniferum strains have been developed which accumulate thebaine a...Oripavine is the major alkaloid of Papaver orientale. It is an important intermediate in the biosynthesis of morphine alkaloids. Recently, new Papaver somniferum strains have been developed which accumulate thebaine and oripavine, but not morphine and codeine. Therefore, the chemistry of oripavine has been studied intensively to synthesize opioid pharmaceuticals such as oxymorphone, naloxone and buprenorphine.展开更多
基金supported by the Central Government Guides the Development of Local Science and Technology(No.14104349).
文摘Twelve previously unidentified triterpenoids(1–12)were isolated from the dichloromethane extract of Alisma orientale(A.orientale).Among these compounds,1 and 2 exhibited a rare 6/6/7/5 tetracyclic ring system,and compound 3 was lanostane,isolated from A.orientale for the first time.The structures,including relative and absolute configurations,were determined through spectroscopic methods,electronic circular dichroism(ECD),Mo2(OAc)4-induced ECD,and single-crystal X-ray diffraction.The anti-pulmonary fibrosis(PF)activity of isolated compounds was evaluated in vitro.The results demonstrated that compounds 1–6 and 11 ameliorated transforming growth factorβ1(TGF-β1)-induced cell damage at 10μmol·L−1(P<0.01).
基金This work was supported by the National Science Foundation of China （No. 30860366） Guizhou Province Municipal Science and Technology Project （No. 2007-6010）.
文摘A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） assay was developed for the determination of the human plasma protein binding of four bioactive ftavonoids （such as orientin and vitexin） in Polygonum orientale. Protein precipitation was used for sample preparation. Equilibrium dialysis technique was applied to determine the plasma protein binding under physiological conditions. The separation was achieved through a Waters C i s column with a mobile phase composed of 0.1% formic acid in acetonitrile and 0.1% aqueous formic acid using step gradient elution at a flow rate of 0.35 mL/min. A Waters ACQUITYTM TQD system was operated under the multiple reaction monitoring （MRM） mode of positive electrospray ionization. All of the recovery, precision, accuracy and stability of the method met the requirements. Good correlations （r 〉 0.99） of the four compounds were found, which suggested that these compounds can be simultaneously determined with acceptable accuracy. Results showed that the plasma protein bindings of the four bioactive flavonoids were in the range of 74-89% over the six concentrations studied. The binding parameters containing protein binding affinity, protein binding dissociation constant, and protein binding site were studied. The maximum ability to bind with protein was also determined in the assay in order to understand the drug-protein binding of each compound better.
基金financially supported by Liaoning Innovative Research Team in University (LNIRT, Grant no.LT2013022)
文摘A rapid, simple and practical high-performance liquid chromatography method coupled with diode array detector（HPLC–DAD） was developed to evaluate the quality of Alisma orientale（Sam.） Juz.through a simultaneous determination of four major active triterpenes using a single standard to determine the multi-components（SSDMCs）. Alisol B 23-acetate was selected as the reference compound for calculating the relative response factors. All calibration curves showed good linearity（R240.9998） within test ranges. RSDs for intra- and inter-day of four analytes were less than 3.6% and 2.3%; the overall recovery was 92.1–110.2%（SSDMC）. The proposed method was successfully applied to quantify the four components in 20 samples from different localities in China. Moreover, significant variations were demonstrated in the content of these compounds. In addition, hierarchical clustering analysis（HCA） and principal components analysis（PCA） were performed to differentiate and classify the samples based on the contents of Alisol C 23-acetate, Alisol A, Alisol A 24-acetate and Alisol B 23-acetate. This simple,rapid, low-cost and reliable HPLC–DAD method using SSDMC is suitable for routine quantitative analysis and quality control of A. orientale（Sam.） Juz.
基金This study was financially supported by Liaoning Innovative Research Team in University(LNIRT,Grant No.LT2013022).
文摘A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale(Sam.)Juz.in rat plasma using diazepam as an internal standard(IS).The plasma samples were extracted by liquideliquid extraction with methyl tert-butyl ether and separated on a Venusil MP C18 column(100 mm×2.1 mm,3.0 mm)(Venusil,China)using gradient elution with the mobile phase consisting of methanol and 0.1%acetic acid in water at a flow rate of 0.4 ml/min.The two analytes were monitored with positive electrospray ionization by multiple reaction monitoring mode(MRM).The lower limit of quantitation was 5.00 ng/ml for alisol A and 5.00 ng/ml for alisol B 23-acetate.The calibration curves were linear in the range of 5.00 e2500 ng/ml for alisol A and 5e2500 ng/ml for alisol B 23-acetate.The mean extraction recoveries were above 63.8%for alisol A and 68.0%for alisol B 23-acetate from biological matrixes.Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria(15%).The validated method was successfully applied to the pharmacokinetic study of alisol A and alisol B 23-acetate in rat plasma after oral administration of alcohol extract of Alismatis Rhizoma.
基金the B-type Strategic Priority Program of the Chinese Academy of Sciences,Grant No.XDB41000000the National Natural Science Foundation of China(41773063,41525015 and 41830214)+1 种基金the Science and Technology Development Fund of Macao(0042/2018/A2)the Pre-research Project on Civil Aerospace Technologies(No.D020101)of CNSA.
文摘The prototype for investigations of formation mechanisms and related geological effects of large impact basins on planetary bodies has been the Orientale basin on the Moon.Its widespread secondaries,light plains,and near-rim melt flows have been well mapped in previous studies.Flow features are also widely associated with secondaries on planetary bodies,but their physical properties are not well constrained.The nature of flow features associated with large impact basins are critically important to understand the emplacement process of basin ejecta,which is one of the most fundamental processes in shaping the shallow crusts of planetary bodies.Here we use multisource remote sensing data to constrain the physical properties of flow features formed by the secondaries of the Orientale basin.The results suggest that such flows are dominated by centimeter-scale fine debris fines;larger boulders are not abundant.The shattering of target materials during the excavation of the Orientale basin,landing impact of ejecta that formed the secondaries,and grain comminution within the flows have substantially reduced particle sizes,forming the fine flows.The discovery of global-wide fine debris flows formed by large impact basins has profound implications to the interpretation of both previously-returned samples and remote sensing data.
基金supported by the National Natural Science Foundation of China(32370484)China Scholarship Council,National Science&Technology Fundamental Resources Investigation Program of China(2019FY101800)the National Animal Collection Resource Center,China.
文摘We review the genus Paraglenurus van der Weele,1909 in China,and provide a new Chinese record:P.scopifer(Gerstaeker,1888)from Orchid Island(Lanyu).Additionally,we describe a new species of Paraglenurus from South Vietnam,i.e.,P.badanoi sp.nov.,which represents the first record of this genus in Indochina.Furthermore,we confirm that the paratypes of P.pumilus(Yang,1997)are actually a distinct new species in the genus Indophanes Banks,1940,i.e.,I.zhiliangi sp.nov.
基金funded by the National Natural Science Foundation of China (No. 32301295 to JW, 32101242 to LM, and 32260253 to LW)High-Level Talents Research Start-Up Project of Hebei University (No. 521100222044 to JW)
文摘Avian alarm calls mediate defenses against brood parasites and predators. These calls facilitate communication among adults and alert nestlings to potential danger. While heterospecific call recognition has been extensively studied in adult birds, nestlings—lacking direct predation experience and heterospecific alarm exposure—represent an ideal system to investigate the response to interspecific warning cues. This study explored the recognition capabilities of 5–6-day-old nestlings in Oriental Reed Warbler (Acrocephalus orientalis), a common host of the Common Cuckoo (Cuculus canorus). We exposed the nestlings to playbacks of alarm calls directed at parasites and raptors from conspecific, Vinous-throated Parrotbill (Sinosuthora webbiana, sympatric species), Isabelline Shrike (Lanius isabellinus, allopatric species) and Common Tailorbird (Orthotomus sutorius, allopatric species) adults. Results indicated that there was no significant difference in the responses of nestlings to the alarm calls of conspecific and allopatric adults directed at cuckoos and sparrowhawks. In addition, interestingly, nestlings significantly reduced their begging in response to conspecific and unfamiliar allopatric Isabelline Shrike and Common Tailorbird alarm calls but exhibited a weak response to the sympatric Vinous-throated Parrotbill. Whether older warbler nestlings with more social experience exhibit stronger responses to the alarm calls of Vinous-throated Parrotbill adults requires further investigation.
基金funded by Gorgan University of Agricultural Sciences and Natural Resources(grant number 9318124503).
文摘Plant species diversity is one of the most widely used indicators in ecosystem management.The relation of species diversity with the size of the sample plot has not been fully determined for Oriental beech forests(Fagus orientalis Lipsky),a widespread species in the Hyrcanian region.Assessing the impacts of plot size on species diversity is fundamental for an ecosystem-based approach to forest management.This study determined the relation of species diversity and plot size by investigating species richness and abundance of both canopy and forest floor.Two hundred and fifty-six sample plots of 625 m^(2) each were layout in a grid pattern across 16 ha.Base plots(25 m×25 m)were integrated in different scales to investigate the effect of plot size on species diversity.The total included nine plots of 0.063,0.125,0.188,0.250,0.375,0.500,0.563,0.750 and 1 ha.Ten biodiversity indices were calculated.The results show that species richness in the different plot sizes was less than the actual value.The estimated value of the Simpson species diversity index was not significantly different from actual values for both canopy and forest floor diversity.The coefficient of variation of this index for the 1-ha sample plot showed the lowest amount across different plot sizes.Inverse Hill species diversity was insignificant difference across different plot sizes with an area greater than 0.500 ha.The modified Hill evenness index for the 1-ha sample size was a correct estimation of the 16-ha for both canopy and forest floor;however,the precision estimation was higher for the canopy layer.All plots greater than 0.250-ha provided an accurate estimation of the Camargo evenness index for forest floor species,but was inaccurate across different plot sizes for the canopy layer.The results indicate that the same plot size did not have the same effect across species diversity measurements.Our results show that correct estimation of species diversity measurements is related to the selection of appropriate indicators and plot size to increase the accuracy of the estimate so that the cost and time of biodiversity management may be reduced.
基金supported by the National Natural Science Foundation of China(NSFC)(32070483)the National Science and Technology Fundamental Resources Investigation Program of China(2019FY101800)。
文摘The study describes a new species,Syndiamesa fanduangularis Fu,sp.nov.,representing the first record of the genus Syndiamesa from the Oriental Region.A detailed description of the new species is provided,including morphological data and illustrations.Molecular data(COI gene)were obtained and deposited in NCBI GenBank,providing a DNA barcode for species identification.The study also provides an updated key to adult males of the genus Syndiamesa globally.
文摘The paper summarizes the research progress of occurrence and comprehensive control of oriental fruit fly in the following aspects ： biological and ecological characteristics, assessment of the risk and suitability, dynamic monitoring and control index, and comprehensive control methods.
文摘AIM： To examine the effects of traditional Chinese medicine （TCM） on alcohol-induced fatty liver in rats. TCM consists of Astragalus membranaceus, Morus alba, Crataegus pinnatifida, Alisma orientale, Salvia miltiorrhiza, and Pueraria lobata. METHODS： The rats were separated randomly into five groups. One （the CD group） was fed a control diet for 10 wk, another （the ED group） fed an ethanol-containing isocaloric liquid diet for 10 wk, and the last three （the TCM group） were fed an ethanol-containing isocaloric liquid diet for 10 wk and dosed orally with TCM （222 mg/kg.d, TCM222; 667 mg/kg·d, TCM667; and 2 000 mg/kg.d, TCM2000, respectively） weekly during the last 4 wk. RESULTS： ED group developed fatty liver according to lipid profile and liver histological findings. Compared with the control group, liver/body weight, serum triglyceride （TG） and total cholesterol （TC）, liver TG and TC, serum alanine aminotransferase （ALT） and aspartic aminotransferase （AST） significantly increased in the ED group. Whereas, in the rats administered with TCM, liver/body weight, serum TG and TC, liver TG and TC, serum ALT and AST were significantly decreased, and the degree of hepatic lipid droplets was markedly improved compared with those in the ED group. CONCLUSION： TCM treatment causes significant reduction in alcohol-induced lipid hepatic accumulation, reversing fatty liver and liver damage, and can be used as a remedy for alcoholic fatty liver.
文摘The artificial nestbox on an Asian White Birch (Betula platyphylla) (1360 m in elevation) was used by a pair of Yellow-rumped Flycatcher (Ficedula zanthopygia) in Beijing, and five eggs were found in the nest in 2005. One was much larger and was identified as the Oriental Cuckoo’s (Cuculus optatus) egg.
文摘Oripavine is the major alkaloid of Papaver orientale. It is an important intermediate in the biosynthesis of morphine alkaloids. Recently, new Papaver somniferum strains have been developed which accumulate thebaine and oripavine, but not morphine and codeine. Therefore, the chemistry of oripavine has been studied intensively to synthesize opioid pharmaceuticals such as oxymorphone, naloxone and buprenorphine.
