The flesh color of oriental melons is an important commercial trait that affects consumer preferences.To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening,carotenoid-targ...The flesh color of oriental melons is an important commercial trait that affects consumer preferences.To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening,carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed(WF)and orange-fleshed(OF)oriental melon cultivars at different stages.The carotenoid-targeted metabolomic analysis indicated thatβ-carotene was the major metabolite that caused differences in flesh color between the two cultivars.Additionally,through KEGG pathway enrichment and weighted gene co-expression network(WGCNA)analysis,metabolic pathways and related transcription factors that are associated with carotenoid metabolism were selected and transcriptome data was verified using RT-qPCR.Finally,the yeast one hybrid and luciferase activity showed that the transcription factor CmWRKY49 could directly bind to the CmPSY1 promoter to activate its expression in the’OF’cultivar.Transient overexpression of CmWRKY49 in’OF’cultivar increased theβ-carotene content,while theβ-carotene content decreased when it was silenced in the same cultivar.This study provides insights into the underlying regulatory network of carotenoid metabolism in oriental melon fruit.展开更多
Lipoxygenases（LOXs） are a group of non-heme,iron-containing enzymes and extensively involved in plant growth and development,ripening and senescence,stress responses,biosynthesis of regulatory molecules and defense ...Lipoxygenases（LOXs） are a group of non-heme,iron-containing enzymes and extensively involved in plant growth and development,ripening and senescence,stress responses,biosynthesis of regulatory molecules and defense reaction.In our previous study,18 LOXs in melon genome were screened and identified,and five 13-LOX genes（CmLOX08,CmLOX10,CmLOX12,CmLOX13 and CmLOX18） were predicted to locate in chloroplast.Phylogenetic analysis result showed that the five genes have high homology with jasmonic acid（JA） biosynthesis-related LOXs from other plants.In addition,promoter analysis revealed that motifs of the five genes participate in gene expression regulated by hormones and stresses.Therefore,we analyzed the expressions of the five genes and LOX activity in leaves of four-leaf stage seedlings of oriental melon cultivar Yumeiren under abiotic stress：wounding,cold,high temperature and hydrogen peroxide（H_2O_2）,and signal molecule treatments：methyl jasmonate（MeJA）,abscisic acid（ABA） and salicylic acid（SA）.Real time qPCR revealed that wounding and H_2O_2 induced the expressions of all the five genes.Only CmLOX08 was induced by cold while only CmLOX13 was suppressed by high temperature.ABA induced the expressions of CmLOXIO and CmLOX12 while inhibited CmLOX13 and CmLOX18.MeJA increased the 3 genes expressions except CmLOX08 and CmLOX13,whereas SA decreased the effect,apart from CmLOX12.All the abiotic stresses and signal molecules treatments increased the LOX activity in leaves of oriental melon.In summary,the results suggest that the five genes have diverse functions in abiotic stress and hormone responses,and might participate in defense response.The data generated in this study will be helpful in subcellular localization and transgenic experiment to understand their precise roles in plant defense response.展开更多
Compared to other melon types, oriental sweet melon （Cucumis melo var. makuwa Makino） is quite a different species with a shorter shelf-life due to its typical climacteric behavior and thin pericarp. The purpose of ...Compared to other melon types, oriental sweet melon （Cucumis melo var. makuwa Makino） is quite a different species with a shorter shelf-life due to its typical climacteric behavior and thin pericarp. The purpose of this experiment is to explore the effects of co- treatment of enhanced freshness formulation （EFF） and 1-methylcyclopropene （1-MCP） on physiological changes and the content of aroma volatile compounds introduced by them of two oriental sweet melon cultivars （Yumeiren and Tianbao） during storage. The melons were stored in incubators with temperature of 15~C and a relative humidity of 85% for 24 d during which fruit quality and related physiological index were measured. Compared to the control, both treatments delayed fruit weight loss rate and kept the fruit firmness, water content and soluble solids content. Ascorbate peroxidase （APX） and phenylalanine ammonia lyase （PAL） activities showed fluctuations in treated melons, while lipoxygenase （LOX） activity （P〈0.01） and malondialdehyde （MDA） content （P〈0.05） decreased compared to control. During the early stage of storage, alcohols and aldehydes were the main volatile compounds, and esters gradually increased during storage. Of all the esters, acetic esters were the main components, followed by oxalic acid esters and other esters. The total content of aroma volatile compounds, esters, alcohols and aldehydes of co-treated melons were all higher than those of 1-MCP treated and control melons. In addition, the aroma volatile peak of co-treated melons occurred later than that of 1-MCP treated and control melons. In summary, co-treatment of EFF and 1-MCP was more beneficial than 1-MCP treatment to delay ripening and senescence, maintain fruit quality, enhance shelf-life and improve levels of aroma volatile compounds.展开更多
基金supported by China Agriculture Research System of MOF and MARA(Grant No.CARS-25).
文摘The flesh color of oriental melons is an important commercial trait that affects consumer preferences.To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening,carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed(WF)and orange-fleshed(OF)oriental melon cultivars at different stages.The carotenoid-targeted metabolomic analysis indicated thatβ-carotene was the major metabolite that caused differences in flesh color between the two cultivars.Additionally,through KEGG pathway enrichment and weighted gene co-expression network(WGCNA)analysis,metabolic pathways and related transcription factors that are associated with carotenoid metabolism were selected and transcriptome data was verified using RT-qPCR.Finally,the yeast one hybrid and luciferase activity showed that the transcription factor CmWRKY49 could directly bind to the CmPSY1 promoter to activate its expression in the’OF’cultivar.Transient overexpression of CmWRKY49 in’OF’cultivar increased theβ-carotene content,while theβ-carotene content decreased when it was silenced in the same cultivar.This study provides insights into the underlying regulatory network of carotenoid metabolism in oriental melon fruit.
基金financially supported by the National Natural Science Foundation of China(31272154)
文摘Lipoxygenases（LOXs） are a group of non-heme,iron-containing enzymes and extensively involved in plant growth and development,ripening and senescence,stress responses,biosynthesis of regulatory molecules and defense reaction.In our previous study,18 LOXs in melon genome were screened and identified,and five 13-LOX genes（CmLOX08,CmLOX10,CmLOX12,CmLOX13 and CmLOX18） were predicted to locate in chloroplast.Phylogenetic analysis result showed that the five genes have high homology with jasmonic acid（JA） biosynthesis-related LOXs from other plants.In addition,promoter analysis revealed that motifs of the five genes participate in gene expression regulated by hormones and stresses.Therefore,we analyzed the expressions of the five genes and LOX activity in leaves of four-leaf stage seedlings of oriental melon cultivar Yumeiren under abiotic stress：wounding,cold,high temperature and hydrogen peroxide（H_2O_2）,and signal molecule treatments：methyl jasmonate（MeJA）,abscisic acid（ABA） and salicylic acid（SA）.Real time qPCR revealed that wounding and H_2O_2 induced the expressions of all the five genes.Only CmLOX08 was induced by cold while only CmLOX13 was suppressed by high temperature.ABA induced the expressions of CmLOXIO and CmLOX12 while inhibited CmLOX13 and CmLOX18.MeJA increased the 3 genes expressions except CmLOX08 and CmLOX13,whereas SA decreased the effect,apart from CmLOX12.All the abiotic stresses and signal molecules treatments increased the LOX activity in leaves of oriental melon.In summary,the results suggest that the five genes have diverse functions in abiotic stress and hormone responses,and might participate in defense response.The data generated in this study will be helpful in subcellular localization and transgenic experiment to understand their precise roles in plant defense response.
基金financially supported by the Key Project of Liaoning Province(2011215003)the Project of the Science and Technology Bureau of Shenyang,China(F12-277-1-26)
文摘Compared to other melon types, oriental sweet melon （Cucumis melo var. makuwa Makino） is quite a different species with a shorter shelf-life due to its typical climacteric behavior and thin pericarp. The purpose of this experiment is to explore the effects of co- treatment of enhanced freshness formulation （EFF） and 1-methylcyclopropene （1-MCP） on physiological changes and the content of aroma volatile compounds introduced by them of two oriental sweet melon cultivars （Yumeiren and Tianbao） during storage. The melons were stored in incubators with temperature of 15~C and a relative humidity of 85% for 24 d during which fruit quality and related physiological index were measured. Compared to the control, both treatments delayed fruit weight loss rate and kept the fruit firmness, water content and soluble solids content. Ascorbate peroxidase （APX） and phenylalanine ammonia lyase （PAL） activities showed fluctuations in treated melons, while lipoxygenase （LOX） activity （P〈0.01） and malondialdehyde （MDA） content （P〈0.05） decreased compared to control. During the early stage of storage, alcohols and aldehydes were the main volatile compounds, and esters gradually increased during storage. Of all the esters, acetic esters were the main components, followed by oxalic acid esters and other esters. The total content of aroma volatile compounds, esters, alcohols and aldehydes of co-treated melons were all higher than those of 1-MCP treated and control melons. In addition, the aroma volatile peak of co-treated melons occurred later than that of 1-MCP treated and control melons. In summary, co-treatment of EFF and 1-MCP was more beneficial than 1-MCP treatment to delay ripening and senescence, maintain fruit quality, enhance shelf-life and improve levels of aroma volatile compounds.
