Ultrafast phenomena induced by femtosecond laser irradiation encompass a range of highly dynamic physical processes,including but not limited to electron excitation,material ablation,plasma generation,and shock wave p...Ultrafast phenomena induced by femtosecond laser irradiation encompass a range of highly dynamic physical processes,including but not limited to electron excitation,material ablation,plasma generation,and shock wave propagation.Unveiling the dynamics of these ultrafast processes is crucial for effectively controlling laser processing.However,many of these phenomena occur on timescales ranging from femtoseconds(fs) to nanoseconds(ns),which presents significant challenges in monitoring and interpretation;thus,ultrafast optical imaging techniques are often required.This paper comprehensively reviews the ultrafast optical imaging methods employed in recent years to monitor various ultrafast processes such as electron excitation,ultrafast ablation,plasma ejection,and shock wave propagation during femtosecond laser processing of metallic,composite,and ceramic materials.These methods can be categorized into two primary types:pump-probe ultrafast optical imaging and single-shot ultrafast optical imaging techniques.The working principles and key findings associated with each type of ultrafast optical imaging technique are described in detail.Finally,the imaging principles,advantages and disadvantages,and application scenarios of various ultrafast imaging technologies are summarized,along with a discussion of future challenges and development directions in this field.展开更多
Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging f...Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging from organic dyes to genetically encoded proteins and nanomaterials, provide unparalleled specificity, sensitivity, and multiplexing capabilities. However, challenges such as brightness, photobleaching, biocompatibility, and emission range continue to drive innovation in probe design and application. This special issue, comprising four review papers and seven original research studies, highlights cutting-edge advancements in fluorescent probe technologies and their transformative roles in super-resolution imaging, in vivo diagnostics, and cancer therapeutics.展开更多
AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical imaging.METHODS: We choose to evaluate the tumor m...AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical imaging.METHODS: We choose to evaluate the tumor metabolic activity using a fluorodeoxyglucose analogue as 2-deoxyglucosone fluorescent probes and to correlate it with tumoral volume(mm^3). Inflammation measuring myeloperoxidase(MPO) activity and reactive oxygen species production was monitored by a bioluminescent(BLI) inflammation probe and related to histological examination and MPO levels by enzyme-linked immunosorbent assay(ELISA) on tumor specimens. The detection and quantitation of these two signals were validated on a xenograft model of human colon adenocarcinoma epithelial cells(HCT116) in nude mice infected with a pks-positive E. coli. The inflammatory BLI signal was validated intra-digestively in the colitisCEABAC10 DSS models, which mimicked Crohn's disease. RESULTS: Using a 2-deoxyglucosone fluorescent probe, we observed a high and specific HCT116 tumor uptake in correlation with tumoral volume(P = 0.0036). Using the inflammation probe targeting MPO, we detected a rapid systemic elimination and a significant increase of the BLI signal in the pks-positive E. coli-infected HCT116 xenograft group(P < 0.005). ELISA confirmed that MPO levels were significantly higher(1556 ± 313.6 vs 234.6 ± 121.6 ng/m L P = 0.001) in xenografts infected with the pathogenic E. coli strain. Moreover, histological examination of tumor samples confirmed massive infiltration of pks-positive E. coli-infected HCT116 tumors by inflammatory cells compared to the uninfected group. These data showed that infection with the pathogenic E. coli strain enhanced inflammation and ROS production in tumors before tumor growth. Moreover, we demonstrated that the intra-digestive monitoring of inflammation is feasible in a reference colitis murine model(CEABAC10/DSS).CONCLUSION: Using BLI and fluorescence optical imaging, we provided tools to better understand hostpathogen interactions at the early stage of disease, such as inflammatory bowel disease and colorectal cancer.展开更多
Silica nanoparticles have been one of the most promising nanosystems for biomedical applications due to their facile surface chemistry and non-toxic nature. However, it is still challenging to effectively deliver them...Silica nanoparticles have been one of the most promising nanosystems for biomedical applications due to their facile surface chemistry and non-toxic nature. However, it is still challenging to effectively deliver them into tumor sites and noninvasively visualize their in vivo biodistribution with excellent sensitivity and accuracy for effective cancer diagnosis. In this study, we design a yolk/shell-structured silica nanosystem ^(64) Cu-NOTAQD@HMSN-PEG-TRC105, which can be employed for tumor vasculature targeting and dual-modality PET/optical imaging, leading to superior targeting specificity, excellentimaging capability and more reliable diagnostic outcomes.By combining vasculature targeting, pH-sensitive drug delivery, and dual-modality imaging into a single platform,as-designed yolk/shell-structured silica nanosystems may be employed for the future image-guided tumor-targeted drug delivery, to further enable cancer theranostics.展开更多
The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural in...The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural information for understanding the brain.Optical approaches can achieve submicron lateral resolution and achieve“optical sectioning”by a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level.Automated whole-brain optical imaging methods based on tissue clearing or histological sectioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural information in a large volume of tissues.Combined with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quantitative profiling of cells,circuits,and blood vessels.In this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.展开更多
Recent years have seen the design and implementation of many optical activatable smart probes.These probes are activatable because they change their optical properties and are smart because they can identify specific ...Recent years have seen the design and implementation of many optical activatable smart probes.These probes are activatable because they change their optical properties and are smart because they can identify specific targets.This broad class of detection agents has allowed previously unperformed visualizations,facilitating the study of diverse biomolecules including enzymes,nucleic acids,ions and reactive oxygen species.Designed to be robust in an in vivo environment,these probes have been used in tissue culture cells and in live small animals.An emerging class of smart probes has been designed to harness the potency of singlet oxygen generating photosensitizers.Combining the discrimination of activatable agents with the toxicity of photosensitizers represents a new and powerful approach to disease treatment.This review highlights some applications of activatable smart probes with a focus on developments of the past decade.展开更多
Recording and identifying faint objects through atmospheric scattering media by an optical system are fundamentally interesting and technologically important.We introduce a comprehensive model that incorporates contri...Recording and identifying faint objects through atmospheric scattering media by an optical system are fundamentally interesting and technologically important.We introduce a comprehensive model that incorporates contributions from target characteristics,atmospheric effects,imaging systems,digital processing,and visual perception to assess the ultimate perceptible limit of geometrical imaging,specifically the angular resolution at the boundary of visible distance.The model allows us to reevaluate the effectiveness of conventional imaging recording,processing,and perception and to analyze the limiting factors that constrain image recognition capabilities in atmospheric media.The simulations were compared with the experimental results measured in a fog chamber and outdoor settings.The results reveal good general agreement between analysis and experiment,pointing out the way to harnessing the physical limit for optical imaging in scattering media.An immediate application of the study is the extension of the image range by an amount of 1.2 times with noise reduction via multiframe averaging,hence greatly enhancing the capability of optical imaging in the atmosphere.展开更多
Internal solitary waves(ISWs)change the roughness of the sea surface,thus producing dark and bright bands in optical images.However,reasons for changes in imaging characteristics with the solar zenith angle remain unc...Internal solitary waves(ISWs)change the roughness of the sea surface,thus producing dark and bright bands in optical images.However,reasons for changes in imaging characteristics with the solar zenith angle remain unclear.In this paper,the optical imaging pattern of ISWs in sunglint under different zenith angles of the light source is investigated by collecting optical images of ISWs through physical simulation.The experiment involves setting 10 zenith angles of the light source,which are divided into area a the optical images of ISWs in the three areas show dark-bright mode,single bright band,and bright-dark mode,which are consistent with those observed by optical remote sensing.In addition,this study analyzed the percentage of the dark and bright areas of the bands and the change in the relative gray difference and found changes in both areas under different zenith angles of the light source.The MODIS and ASAR images display a similar brightness-darkness distance of the same ISWs.Therefore,the relationship between the brightness-darkness distance and the characteristic half-width of ISWs is determined in accordance with the eKdV theory and the imaging mechanism of ISWs of the SAR image.Overall,the relationship between them in the experiment is almost consistent with the theoretical result.展开更多
Real-time polarization medium-wave infrared(MIR)optical imaging systems enable the acquisition of infrared and polarization information for a target.At present,real-time polarization MIR devices face the following pro...Real-time polarization medium-wave infrared(MIR)optical imaging systems enable the acquisition of infrared and polarization information for a target.At present,real-time polarization MIR devices face the following problems:poor real-time performance,low transmission and high requirements for fabrication and integration.Herein,we aim to improve the performance of real-time polarization imaging systems in the MIR waveband and solve the above-mentioned defects.Therefore,we propose a MIR polarization imaging system to achieve real-time polarization-modulated imaging with high transmission as well as improved performance based on a pixel-wise metasurface micro-polarization array(PMMPA).The PMMPA element comprises several linear polarization(LP)filters with different polarization angles.The optimization results demonstrate that the transmittance of the center field of view for the LP filters is up to 77%at a wavelength of4.0μm and an extinction ratio of 88 d B.In addition,a near-diffraction-limited real-time MIR imaging optical system is designed with a field of view of 5°and an F-number of 2.The simulation results show that an MIR polarization imaging system with excellent real-time performance and high transmission is achieved by using the optimized PMMPA element.Therefore,the method is compatible with the available optical system design technologies and provides a way to realize real-time polarization imaging in MIR wavebands.展开更多
Computational optical imaging is an interdisciplinary subject integrating optics, mathematics, and information technology. It introduces information processing into optical imaging and combines it with intelligent com...Computational optical imaging is an interdisciplinary subject integrating optics, mathematics, and information technology. It introduces information processing into optical imaging and combines it with intelligent computing, subverting the imaging mechanism of traditional optical imaging which only relies on orderly information transmission. To meet the high-precision requirements of traditional optical imaging for optical processing and adjustment, as well as to solve its problems of being sensitive to gravity and temperature in use, we establish an optical imaging system model from the perspective of computational optical imaging and studies how to design and solve the imaging consistency problem of optical system under the influence of gravity, thermal effect, stress, and other external environment to build a high robustness optical system. The results show that the high robustness interval of the optical system exists and can effectively reduce the sensitivity of the optical system to the disturbance of each link, thus realizing the high robustness of optical imaging.展开更多
Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells...Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells with spatial information.Therefore,in this review,we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals.Then,we describe optical imaging to achieve threedimensional spatial discrimination for biological tisutes.Due to the milky appearance of tissues,the spatial information burred deep in the whole organ.Fortunately,strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.展开更多
BACKGROUND Radionuclides produce Cherenkov radiation(CR),which can potentially activate photosensitizers(PSs)in phototherapy.Several groups have studied Cherenkov energy transfer to PSs using optical imaging;however,c...BACKGROUND Radionuclides produce Cherenkov radiation(CR),which can potentially activate photosensitizers(PSs)in phototherapy.Several groups have studied Cherenkov energy transfer to PSs using optical imaging;however,cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge.Many laboratories face the need for expensive dedicated equipment.AIM To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.METHODS The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs.To mitigate the need for expensive dedicated equipment and achieve the aim of the study,we developed a method that utilizes a chargecoupled device optical imaging system and appropriate long-pass filters of different wavelengths(manual sequential application of long-pass filters of 515,580,645,700,750,and 800 nm).Tetrakis(4-carboxyphenyl)porphyrin(TCPP)was utilized as a model PS.Different doses of copper-64(^(64)CuCl_(2))(4,2,and 1 mCi)were used as CR-producing radionuclides.Imaging and data acquisition were performed 0.5 h after sample preparation.Differential image analysis was conducted by using ImageJ software(National Institutes of Health)to visually evaluate TCPP fluorescence.RESULTS The maximum absorbance of TCPP was at 390-430 nm,and the emission peak was at 670 nm.The CR and CRinduced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above.The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity(total flux)difference between^(64)CuCl_(2)+TCPP and^(64)CuCl_(2).Moreover,the differential fluorescence images of TCPP were obtained by subtracting the^(64)CuCl_(2)image from the^(64)CuCl_(2)+TCPP image.The experimental results considering different^(64)CuCl_(2)doses showed a dosedependent trend.These results demonstrate that a bioluminescence imaging device coupled with different longpass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.CONCLUSION This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.展开更多
Adoptive T-cell therapy(ACT),which is an important type of live cell therapy,has achieved unprecedented success in treating hematological malignancies.Recent studies have shown that ACT is also a promising treatment f...Adoptive T-cell therapy(ACT),which is an important type of live cell therapy,has achieved unprecedented success in treating hematological malignancies.Recent studies have shown that ACT is also a promising treatment for solid tumors.Visualizing the in vivo fates(distribution,homing,infiltration,proliferation,and exhaustion)of the immune cells used for ACT(ACT immune cells)is of great importance to promote basic research and clinical translation of ACT.Optical imaging techniques,including bioluminescence,fluorescence,and photoacoustic imaging,have the advantages of high sensitivity,high spatiotemporal resolution,minimal exposure to harmful radiation,and simple instrumentation.Recently,various types of optical imaging probes,including bioluminescence,fluorescence,and photoacoustic imaging probes,have been used to visualize ACT immune cells in vivo and evaluate the molecular mechanism,efficacy,and side effects of ACT.In this review,the optical imaging probes and labeling methods that have been used for in vivo visualization ofACT immune cells are summarized,and the opportunities and challenges of using optical imaging to visualize ACT immune cells in vivo are discussed.展开更多
Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images o...Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter.The human retina is a thin and multiple layer tissue,and the layer of capillaries less than10 μm in diameter only exists in the inner nuclear layer.The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod＆cone cell surface is tens of micrometers,which varies from person to person.Therefore,determining reasonable capillary layer（CL） position in different human eyes is very difficult.In this paper,we propose a method to determine the position of retinal CL based on the rod＆cone cell layer.The public positions of CL are recognized with 15 subjects from 40 to 59 years old,and the imaging planes of CL are calculated by the effective focal length of the human eye.High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system（LCAOS） validate our method.All of the subjects＇ CLs have public positions from 127 μm to 147 μm from the rod＆cone cell layer,which is influenced by the depth of focus.展开更多
Bioluminescence tomography(BLT)is a novel opt ical molecular imaging technique that advanced the conventional planar bioluminescence imaging(BLI)into a quantifiable three-dimensional(3D)approach in preclinical living ...Bioluminescence tomography(BLT)is a novel opt ical molecular imaging technique that advanced the conventional planar bioluminescence imaging(BLI)into a quantifiable three-dimensional(3D)approach in preclinical living animal studies in oncology.In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately,the prior structural information is com-monly obtained from X ray computed tomography(CT).This strategy requires a complicated hybrid imaging system,extensive post imaging analysis and involvement of ionizing radiation.Moreover,the overall robustness highly depends on the fusion accuracy between the optical and structural information.Here,we present a pure optical bioluminescence tomographic(POBT)system and a novel BLT workfow based on multi-view projection acquisition and 3D surface reconstruction.This met hod can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images,so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT.The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomo-graphic(DMT)system and a commercialized optical imaging system(IVIS Spectrum)using three breast cancer xenografts.The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system(P>0.05)in much shorter data analysis time.It also offered significantly better accuracy comparing with the IVIS system(P<0.04)without sacrificing too much time.展开更多
Water-related optical imaging constitutes a pivotal domain within the field of water-related optics and vision,furnishing essential tools for comprehending the water-related environment,harnessing water resources,and ...Water-related optical imaging constitutes a pivotal domain within the field of water-related optics and vision,furnishing essential tools for comprehending the water-related environment,harnessing water resources,and safeguarding water ecology.Its merits encompass facile target detection,elevated imaging resolution,and copious information content.Nonetheless,the formidable challenge of attaining clear images in water-related environments is challenging due to the water's absorption and scattering properties.Substantial strides have been achieved in recent years through advancements in software and hardware optical imaging and processing techniques.This review offers a comprehensive exploration of the present state of water-related optical imaging,spanning historical,theoretical,and methodological dimensions.A retrospective analysis of the historical evolution of water-related optical imaging is presented,accompanied by a detailed exposition of the operational principles and the latest noteworthy advancements in various categories of water-related optical imaging.Furthermore,a meticulous comparative analysis of these methodologies is undertaken.These approaches not only enhance water-related optical imaging efficacy but also furnish robust support for scientific inquiry and resource exploitation in water-related environments.This review enhances the comprehension of advancements in water-related optical imaging research,acting as a crucial touchpoint for future explorations and innovations.Water-related optical imaging is critical for comprehending marine ecosystems,supporting environmental conservation,and improving industry efficiency.展开更多
Airborne optical imaging can flexibly obtain the intuitive information of the observed scene from the air,which plays an important role of modern optical remote sensing technology.Higher resolution,longer imaging dist...Airborne optical imaging can flexibly obtain the intuitive information of the observed scene from the air,which plays an important role of modern optical remote sensing technology.Higher resolution,longer imaging distance,and broader coverage are the unwavering pursuits in this research field.Nevertheless,the imaging environment during aerial flights brings about multi-source dynamic interferences such as temperature,air pressure,and complex movements,which forms a serious contradiction with the requirements of precision and relative staticity in optical imaging.As the birthplace of Chinese optical industry,the Changchun Institute of Optics,Fine Mechanics and Physics(CIOMP)has conducted the research on airborne optical imaging for decades,resulting in rich innovative achievements,completed research conditions,and exploring a feasible development path.This article provides an overview of the innovative work of CiOMP in the field of airborne optical imaging,sorts out the milestone nodes,and predicts the future development direction of this discipline,with the aim of providing inspiration for related research.展开更多
The Pomacea canaliculata,also known as apple snail,is an invasive species in China,posing a serious threat to agricultural production and public health.To prevent its rampant infestation,we developed a highly efficien...The Pomacea canaliculata,also known as apple snail,is an invasive species in China,posing a serious threat to agricultural production and public health.To prevent its rampant infestation,we developed a highly efficient snail-killing lead compound,1-(4-chlorophenyl)-3-(pyridin-3-yl)urea(PBQ).However,the killing mechanism of PBQ remains unclear.To elucidate this,two novel compounds,B-PBQ and J-PBQ,were developed through structural modification of PBQ and were used,through both protein pull-down assay and confocal laser scanning microscopy imaging,to identify and confirm that phosphofructokinase(PFK)in the apple snail is the primary target protein of PBQ.Further studies revealed that PBQ successfully inhibits the activity,content,and expression of PFK in apple snail,disrupting the glycolysis pathway and leading to abnormal energy production.These disruptions impair the snail's tolerance to low oxygen,resulting in its efficient eradication.These findings offer a promising direction for developing next-generation PFK-targeted snail-killing pesticides.Furthermore,the strategy of combining pull-down assays with optical imaging used in this work provides a novel approach for the exploration of mechanisms of other pesticide candidates.展开更多
Self-powered photoelectrochemical(PEC)photodetectors hold great promise for underwater optical applications,yet suffer from sluggish carrier dynamics and limited stability.Herein,high-performance,selfpowered,and stabl...Self-powered photoelectrochemical(PEC)photodetectors hold great promise for underwater optical applications,yet suffer from sluggish carrier dynamics and limited stability.Herein,high-performance,selfpowered,and stable PEC ultraviolet(UV)photodetectors were fabricated using metalorganicframework derived ZnO nanocages(NCs).These topography-engineered ZnO NCs synchronously enhance UV absorption,facilitate photogeneration carrier separation,and promote charge transfer at the ZnO/electrolyte interface,thus optimizing the overall photoresponse performance.The ZnO NCs-based PEC device achieves an ultrahigh responsivity of 300.6 mA/W under 365 nm UV light irradiation,a fast response time of 10/20 ms,outstanding spectra selectivity(UV/visible rejection ratio of 2000),and excellent cycling stability(10,000 cycles),which is one of the best reported PEC UV photodetectors.Furthermore,the self-powered ZnO-based PEC PDs have good underwater optical imaging capability.This work provides a new idea for designing high-performance UV photodetectors for application in underwater environments.展开更多
AIM:To evaluate the choroidopathy in patients with systemic lupus erythematosus(SLE)using enhanced depth imaging spectral domain optical coherence tomography(EDI SD-OCT)and optical coherence tomography angiography(OCT...AIM:To evaluate the choroidopathy in patients with systemic lupus erythematosus(SLE)using enhanced depth imaging spectral domain optical coherence tomography(EDI SD-OCT)and optical coherence tomography angiography(OCTA).METHODS:A total of 74 patients with SLE and 40 healthy volunteers were included in this cross-sectional study.SLE patients were further divided into three subgroups based on clinical and blood biochemistry findings.Ocular parameters obtained on ophthalmologic examination and optical imaging(EDI SD-OCT and OCTA)included the best corrected distance visual acuity(logMAR CDVA),subfoveal choroidal thickness(SCT),choroidal vascularity index(CVI)and vessel density(VD)of superficial capillary plexus(SCP)and deep capillary plexus(DCP).RESULTS:SLE patients had significantly lower values for CVI and VD of DCP(DVD)than control subjects.Amongst SLE patients,gender and chloroquine dose were found to be independent determinants of CVI while age predicted SCT.Steroid dose was a significant predictor for foveal VD of SCP(SVD),chloroquine dose for parafoveal SVD,gender for total DVD,and gender and steroid dose for perifoveal DVD.No correlation of logMAR CDVA and SCT was noted between SLE patients and control subjects.No correlation of SCT was noted with disease duration,Systemic Lupus Erythematosus Disease Activity Index(SLEDAI)score,hydroxychloroquine(HCQ)dose or steroid dose.No correlation of CVI was noted with patient age,disease duration,SLEDAI score,HCQ dose or steroid dose.No significant difference was noted between SLE subgroups in terms of any of the ocular parameters studied.CONCLUSION:The findings reveal the presence of ocular findings suggestive of early onset choroidopathy on EDI SD-OCT and OCTA in SLE patients,in the absence of ocular manifestations or active disease.展开更多
基金supported by the National Key R&D Program of China(No.2022YFB4601601)the Key R&D Program of Guangxi Province,China(No.GKAB23026101)+1 种基金the Base,Talent Special Project of the Guangxi Science and Technology Plan Project(No.Gui Ke AD23026149)Guangxi Natural Science Foundation,China(No.2023GXNSFBA026287)
文摘Ultrafast phenomena induced by femtosecond laser irradiation encompass a range of highly dynamic physical processes,including but not limited to electron excitation,material ablation,plasma generation,and shock wave propagation.Unveiling the dynamics of these ultrafast processes is crucial for effectively controlling laser processing.However,many of these phenomena occur on timescales ranging from femtoseconds(fs) to nanoseconds(ns),which presents significant challenges in monitoring and interpretation;thus,ultrafast optical imaging techniques are often required.This paper comprehensively reviews the ultrafast optical imaging methods employed in recent years to monitor various ultrafast processes such as electron excitation,ultrafast ablation,plasma ejection,and shock wave propagation during femtosecond laser processing of metallic,composite,and ceramic materials.These methods can be categorized into two primary types:pump-probe ultrafast optical imaging and single-shot ultrafast optical imaging techniques.The working principles and key findings associated with each type of ultrafast optical imaging technique are described in detail.Finally,the imaging principles,advantages and disadvantages,and application scenarios of various ultrafast imaging technologies are summarized,along with a discussion of future challenges and development directions in this field.
文摘Fluorescent probes have revolutionized optical imaging and biosensing by enabling real-time visualization, quantification, and tracking of biological processes at molecular and cellular levels. These probes, ranging from organic dyes to genetically encoded proteins and nanomaterials, provide unparalleled specificity, sensitivity, and multiplexing capabilities. However, challenges such as brightness, photobleaching, biocompatibility, and emission range continue to drive innovation in probe design and application. This special issue, comprising four review papers and seven original research studies, highlights cutting-edge advancements in fluorescent probe technologies and their transformative roles in super-resolution imaging, in vivo diagnostics, and cancer therapeutics.
基金Supported by Veziant J was supported by«année-recherche»grants from the Ministère de la Santéand the Facultéde Médecine de Clermont-FerrandGagnière J was supported by a“Nuovo Soldati Foundation for Cancer Research”grant.
文摘AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical imaging.METHODS: We choose to evaluate the tumor metabolic activity using a fluorodeoxyglucose analogue as 2-deoxyglucosone fluorescent probes and to correlate it with tumoral volume(mm^3). Inflammation measuring myeloperoxidase(MPO) activity and reactive oxygen species production was monitored by a bioluminescent(BLI) inflammation probe and related to histological examination and MPO levels by enzyme-linked immunosorbent assay(ELISA) on tumor specimens. The detection and quantitation of these two signals were validated on a xenograft model of human colon adenocarcinoma epithelial cells(HCT116) in nude mice infected with a pks-positive E. coli. The inflammatory BLI signal was validated intra-digestively in the colitisCEABAC10 DSS models, which mimicked Crohn's disease. RESULTS: Using a 2-deoxyglucosone fluorescent probe, we observed a high and specific HCT116 tumor uptake in correlation with tumoral volume(P = 0.0036). Using the inflammation probe targeting MPO, we detected a rapid systemic elimination and a significant increase of the BLI signal in the pks-positive E. coli-infected HCT116 xenograft group(P < 0.005). ELISA confirmed that MPO levels were significantly higher(1556 ± 313.6 vs 234.6 ± 121.6 ng/m L P = 0.001) in xenografts infected with the pathogenic E. coli strain. Moreover, histological examination of tumor samples confirmed massive infiltration of pks-positive E. coli-infected HCT116 tumors by inflammatory cells compared to the uninfected group. These data showed that infection with the pathogenic E. coli strain enhanced inflammation and ROS production in tumors before tumor growth. Moreover, we demonstrated that the intra-digestive monitoring of inflammation is feasible in a reference colitis murine model(CEABAC10/DSS).CONCLUSION: Using BLI and fluorescence optical imaging, we provided tools to better understand hostpathogen interactions at the early stage of disease, such as inflammatory bowel disease and colorectal cancer.
基金supported,in part,by the University of Wisconsin–Madisonthe National Institutes of Health (P30CA014520 and T32CA009206)the American Cancer Society (125246-RSG-13-099-01-CCE)
文摘Silica nanoparticles have been one of the most promising nanosystems for biomedical applications due to their facile surface chemistry and non-toxic nature. However, it is still challenging to effectively deliver them into tumor sites and noninvasively visualize their in vivo biodistribution with excellent sensitivity and accuracy for effective cancer diagnosis. In this study, we design a yolk/shell-structured silica nanosystem ^(64) Cu-NOTAQD@HMSN-PEG-TRC105, which can be employed for tumor vasculature targeting and dual-modality PET/optical imaging, leading to superior targeting specificity, excellentimaging capability and more reliable diagnostic outcomes.By combining vasculature targeting, pH-sensitive drug delivery, and dual-modality imaging into a single platform,as-designed yolk/shell-structured silica nanosystems may be employed for the future image-guided tumor-targeted drug delivery, to further enable cancer theranostics.
基金supported by the STI2030-Major Projects(2021ZD0201001 and 2021ZD0201000)the National Natural Science Foundation of China(81827901 and 32192412).
文摘The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected neurons.Precise dissection of neural circuits at the mesoscopic level can provide important structural information for understanding the brain.Optical approaches can achieve submicron lateral resolution and achieve“optical sectioning”by a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic level.Automated whole-brain optical imaging methods based on tissue clearing or histological sectioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural information in a large volume of tissues.Combined with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quantitative profiling of cells,circuits,and blood vessels.In this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.
基金supported by Canadian Cancer Society Grant#018510 through the National Cancer Institute of Canada.
文摘Recent years have seen the design and implementation of many optical activatable smart probes.These probes are activatable because they change their optical properties and are smart because they can identify specific targets.This broad class of detection agents has allowed previously unperformed visualizations,facilitating the study of diverse biomolecules including enzymes,nucleic acids,ions and reactive oxygen species.Designed to be robust in an in vivo environment,these probes have been used in tissue culture cells and in live small animals.An emerging class of smart probes has been designed to harness the potency of singlet oxygen generating photosensitizers.Combining the discrimination of activatable agents with the toxicity of photosensitizers represents a new and powerful approach to disease treatment.This review highlights some applications of activatable smart probes with a focus on developments of the past decade.
基金supported by the National Natural Science Foundation of China(Grant Nos.61991452 and 12074444)the Guangdong Major Project of Basic and Applied Basic Research(Grant No.2020B0301030009)the National Key Research and Development Program of China(Grant Nos.2022YFA1404300 and 2020YFC2007102).
文摘Recording and identifying faint objects through atmospheric scattering media by an optical system are fundamentally interesting and technologically important.We introduce a comprehensive model that incorporates contributions from target characteristics,atmospheric effects,imaging systems,digital processing,and visual perception to assess the ultimate perceptible limit of geometrical imaging,specifically the angular resolution at the boundary of visible distance.The model allows us to reevaluate the effectiveness of conventional imaging recording,processing,and perception and to analyze the limiting factors that constrain image recognition capabilities in atmospheric media.The simulations were compared with the experimental results measured in a fog chamber and outdoor settings.The results reveal good general agreement between analysis and experiment,pointing out the way to harnessing the physical limit for optical imaging in scattering media.An immediate application of the study is the extension of the image range by an amount of 1.2 times with noise reduction via multiframe averaging,hence greatly enhancing the capability of optical imaging in the atmosphere.
基金National Natural Science Foundation of China (Nos.61871353 and 42006164)for their support。
文摘Internal solitary waves(ISWs)change the roughness of the sea surface,thus producing dark and bright bands in optical images.However,reasons for changes in imaging characteristics with the solar zenith angle remain unclear.In this paper,the optical imaging pattern of ISWs in sunglint under different zenith angles of the light source is investigated by collecting optical images of ISWs through physical simulation.The experiment involves setting 10 zenith angles of the light source,which are divided into area a the optical images of ISWs in the three areas show dark-bright mode,single bright band,and bright-dark mode,which are consistent with those observed by optical remote sensing.In addition,this study analyzed the percentage of the dark and bright areas of the bands and the change in the relative gray difference and found changes in both areas under different zenith angles of the light source.The MODIS and ASAR images display a similar brightness-darkness distance of the same ISWs.Therefore,the relationship between the brightness-darkness distance and the characteristic half-width of ISWs is determined in accordance with the eKdV theory and the imaging mechanism of ISWs of the SAR image.Overall,the relationship between them in the experiment is almost consistent with the theoretical result.
基金Project supported by the National Key R&D Program of China(Grant No.SKLA02020001A05)。
文摘Real-time polarization medium-wave infrared(MIR)optical imaging systems enable the acquisition of infrared and polarization information for a target.At present,real-time polarization MIR devices face the following problems:poor real-time performance,low transmission and high requirements for fabrication and integration.Herein,we aim to improve the performance of real-time polarization imaging systems in the MIR waveband and solve the above-mentioned defects.Therefore,we propose a MIR polarization imaging system to achieve real-time polarization-modulated imaging with high transmission as well as improved performance based on a pixel-wise metasurface micro-polarization array(PMMPA).The PMMPA element comprises several linear polarization(LP)filters with different polarization angles.The optimization results demonstrate that the transmittance of the center field of view for the LP filters is up to 77%at a wavelength of4.0μm and an extinction ratio of 88 d B.In addition,a near-diffraction-limited real-time MIR imaging optical system is designed with a field of view of 5°and an F-number of 2.The simulation results show that an MIR polarization imaging system with excellent real-time performance and high transmission is achieved by using the optimized PMMPA element.Therefore,the method is compatible with the available optical system design technologies and provides a way to realize real-time polarization imaging in MIR wavebands.
文摘Computational optical imaging is an interdisciplinary subject integrating optics, mathematics, and information technology. It introduces information processing into optical imaging and combines it with intelligent computing, subverting the imaging mechanism of traditional optical imaging which only relies on orderly information transmission. To meet the high-precision requirements of traditional optical imaging for optical processing and adjustment, as well as to solve its problems of being sensitive to gravity and temperature in use, we establish an optical imaging system model from the perspective of computational optical imaging and studies how to design and solve the imaging consistency problem of optical system under the influence of gravity, thermal effect, stress, and other external environment to build a high robustness optical system. The results show that the high robustness interval of the optical system exists and can effectively reduce the sensitivity of the optical system to the disturbance of each link, thus realizing the high robustness of optical imaging.
基金supported by the National Science and Technology Innovation 2030 Grant No. (2021ZD0200104)National Nature Science Foundation of China (81871082).
文摘Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells with spatial information.Therefore,in this review,we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals.Then,we describe optical imaging to achieve threedimensional spatial discrimination for biological tisutes.Due to the milky appearance of tissues,the spatial information burred deep in the whole organ.Fortunately,strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.
基金This study was reviewed and approved by the Institutional Review Board of National Institutes for Quantum Science and Technology,No.07-1064-28.No animals or animal-derived samples or patients or patient-derived samples were included in this study.
文摘BACKGROUND Radionuclides produce Cherenkov radiation(CR),which can potentially activate photosensitizers(PSs)in phototherapy.Several groups have studied Cherenkov energy transfer to PSs using optical imaging;however,cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge.Many laboratories face the need for expensive dedicated equipment.AIM To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.METHODS The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs.To mitigate the need for expensive dedicated equipment and achieve the aim of the study,we developed a method that utilizes a chargecoupled device optical imaging system and appropriate long-pass filters of different wavelengths(manual sequential application of long-pass filters of 515,580,645,700,750,and 800 nm).Tetrakis(4-carboxyphenyl)porphyrin(TCPP)was utilized as a model PS.Different doses of copper-64(^(64)CuCl_(2))(4,2,and 1 mCi)were used as CR-producing radionuclides.Imaging and data acquisition were performed 0.5 h after sample preparation.Differential image analysis was conducted by using ImageJ software(National Institutes of Health)to visually evaluate TCPP fluorescence.RESULTS The maximum absorbance of TCPP was at 390-430 nm,and the emission peak was at 670 nm.The CR and CRinduced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above.The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity(total flux)difference between^(64)CuCl_(2)+TCPP and^(64)CuCl_(2).Moreover,the differential fluorescence images of TCPP were obtained by subtracting the^(64)CuCl_(2)image from the^(64)CuCl_(2)+TCPP image.The experimental results considering different^(64)CuCl_(2)doses showed a dosedependent trend.These results demonstrate that a bioluminescence imaging device coupled with different longpass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.CONCLUSION This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.
基金Natural Science Foundation of China,Grant/Award Number:92159304Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province,Grant/Award Number:2020B1212060051+3 种基金Basic and Applied Basic Research Foundation of Guangdong Province,Grant/Award Numbers:2022A1515010384,2023A1515010747CAS Key Laboratory of Health Informatics,Grant/Award Number:2011DP173015Science and Technology Key Project of Shenzhen,Grant/Award Numbers:JCYJ20190812163614809,JCYJ20200109114612308,JCYJ20210324120011030Shenzhen Key Laboratory of Ultrasound Imaging and Therapy,Grant/Award Number:ZDSYS201802061806314。
文摘Adoptive T-cell therapy(ACT),which is an important type of live cell therapy,has achieved unprecedented success in treating hematological malignancies.Recent studies have shown that ACT is also a promising treatment for solid tumors.Visualizing the in vivo fates(distribution,homing,infiltration,proliferation,and exhaustion)of the immune cells used for ACT(ACT immune cells)is of great importance to promote basic research and clinical translation of ACT.Optical imaging techniques,including bioluminescence,fluorescence,and photoacoustic imaging,have the advantages of high sensitivity,high spatiotemporal resolution,minimal exposure to harmful radiation,and simple instrumentation.Recently,various types of optical imaging probes,including bioluminescence,fluorescence,and photoacoustic imaging probes,have been used to visualize ACT immune cells in vivo and evaluate the molecular mechanism,efficacy,and side effects of ACT.In this review,the optical imaging probes and labeling methods that have been used for in vivo visualization ofACT immune cells are summarized,and the opportunities and challenges of using optical imaging to visualize ACT immune cells in vivo are discussed.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11174274,11174279,61205021,11204299,61475152,and 61405194)
文摘Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter.The human retina is a thin and multiple layer tissue,and the layer of capillaries less than10 μm in diameter only exists in the inner nuclear layer.The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod＆cone cell surface is tens of micrometers,which varies from person to person.Therefore,determining reasonable capillary layer（CL） position in different human eyes is very difficult.In this paper,we propose a method to determine the position of retinal CL based on the rod＆cone cell layer.The public positions of CL are recognized with 15 subjects from 40 to 59 years old,and the imaging planes of CL are calculated by the effective focal length of the human eye.High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system（LCAOS） validate our method.All of the subjects＇ CLs have public positions from 127 μm to 147 μm from the rod＆cone cell layer,which is influenced by the depth of focus.
基金the National Basic Research Program of China(973 Program)under Grant No.2015CB755500the National Natural Science Foundation of China under Grant No.81227901,61231004,81527805 and 61401462+3 种基金the Scienti¯c Research and Equipment Development Project of the Chinese Academy of Sciences under Grant No.YZ201359the Chinese Academy of Sciences under Grant No.KGZD-EW-T03the Chinese Academy of Sciences Fellowship for Young International Scientists under Grant No.2013Y1GA0004the Project funded by China Postdoctoral Science Foundation under Grant Nos.2014M550881,2015T80155.
文摘Bioluminescence tomography(BLT)is a novel opt ical molecular imaging technique that advanced the conventional planar bioluminescence imaging(BLI)into a quantifiable three-dimensional(3D)approach in preclinical living animal studies in oncology.In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately,the prior structural information is com-monly obtained from X ray computed tomography(CT).This strategy requires a complicated hybrid imaging system,extensive post imaging analysis and involvement of ionizing radiation.Moreover,the overall robustness highly depends on the fusion accuracy between the optical and structural information.Here,we present a pure optical bioluminescence tomographic(POBT)system and a novel BLT workfow based on multi-view projection acquisition and 3D surface reconstruction.This met hod can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images,so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT.The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomo-graphic(DMT)system and a commercialized optical imaging system(IVIS Spectrum)using three breast cancer xenografts.The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system(P>0.05)in much shorter data analysis time.It also offered significantly better accuracy comparing with the IVIS system(P<0.04)without sacrificing too much time.
基金supported by the National Key Research and Development Program of China(Grant Nos.2022YFC2808000,2022YFC2808003)Fundamental Research Funds for the Central Universities(Grant No.D5000220481)Natural Science Foundation of Shaanxi Province,China(Grant No.2024JC-YBMS-468)。
文摘Water-related optical imaging constitutes a pivotal domain within the field of water-related optics and vision,furnishing essential tools for comprehending the water-related environment,harnessing water resources,and safeguarding water ecology.Its merits encompass facile target detection,elevated imaging resolution,and copious information content.Nonetheless,the formidable challenge of attaining clear images in water-related environments is challenging due to the water's absorption and scattering properties.Substantial strides have been achieved in recent years through advancements in software and hardware optical imaging and processing techniques.This review offers a comprehensive exploration of the present state of water-related optical imaging,spanning historical,theoretical,and methodological dimensions.A retrospective analysis of the historical evolution of water-related optical imaging is presented,accompanied by a detailed exposition of the operational principles and the latest noteworthy advancements in various categories of water-related optical imaging.Furthermore,a meticulous comparative analysis of these methodologies is undertaken.These approaches not only enhance water-related optical imaging efficacy but also furnish robust support for scientific inquiry and resource exploitation in water-related environments.This review enhances the comprehension of advancements in water-related optical imaging research,acting as a crucial touchpoint for future explorations and innovations.Water-related optical imaging is critical for comprehending marine ecosystems,supporting environmental conservation,and improving industry efficiency.
基金supported by the Strategic Priority Research Program of the ChineseAcademy of Sciences,Grant No.XDB1050000.
文摘Airborne optical imaging can flexibly obtain the intuitive information of the observed scene from the air,which plays an important role of modern optical remote sensing technology.Higher resolution,longer imaging distance,and broader coverage are the unwavering pursuits in this research field.Nevertheless,the imaging environment during aerial flights brings about multi-source dynamic interferences such as temperature,air pressure,and complex movements,which forms a serious contradiction with the requirements of precision and relative staticity in optical imaging.As the birthplace of Chinese optical industry,the Changchun Institute of Optics,Fine Mechanics and Physics(CIOMP)has conducted the research on airborne optical imaging for decades,resulting in rich innovative achievements,completed research conditions,and exploring a feasible development path.This article provides an overview of the innovative work of CiOMP in the field of airborne optical imaging,sorts out the milestone nodes,and predicts the future development direction of this discipline,with the aim of providing inspiration for related research.
基金supported by the National Natural Science Foundation of China(82072309,22067019,22367023,32160236)the Major Science and Technology Project of Yunnan Province(202402AE090006)+1 种基金the Yunnan Provincial Science and Technology Department-Yunnan University Joint Special Project(202201BF070001-001)the Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai(2023–2025)Key Discipline Project(GWV111.1-12)。
文摘The Pomacea canaliculata,also known as apple snail,is an invasive species in China,posing a serious threat to agricultural production and public health.To prevent its rampant infestation,we developed a highly efficient snail-killing lead compound,1-(4-chlorophenyl)-3-(pyridin-3-yl)urea(PBQ).However,the killing mechanism of PBQ remains unclear.To elucidate this,two novel compounds,B-PBQ and J-PBQ,were developed through structural modification of PBQ and were used,through both protein pull-down assay and confocal laser scanning microscopy imaging,to identify and confirm that phosphofructokinase(PFK)in the apple snail is the primary target protein of PBQ.Further studies revealed that PBQ successfully inhibits the activity,content,and expression of PFK in apple snail,disrupting the glycolysis pathway and leading to abnormal energy production.These disruptions impair the snail's tolerance to low oxygen,resulting in its efficient eradication.These findings offer a promising direction for developing next-generation PFK-targeted snail-killing pesticides.Furthermore,the strategy of combining pull-down assays with optical imaging used in this work provides a novel approach for the exploration of mechanisms of other pesticide candidates.
基金the Natural Science Foundation of Heilongjiang Province,China(No.PL2024E001).
文摘Self-powered photoelectrochemical(PEC)photodetectors hold great promise for underwater optical applications,yet suffer from sluggish carrier dynamics and limited stability.Herein,high-performance,selfpowered,and stable PEC ultraviolet(UV)photodetectors were fabricated using metalorganicframework derived ZnO nanocages(NCs).These topography-engineered ZnO NCs synchronously enhance UV absorption,facilitate photogeneration carrier separation,and promote charge transfer at the ZnO/electrolyte interface,thus optimizing the overall photoresponse performance.The ZnO NCs-based PEC device achieves an ultrahigh responsivity of 300.6 mA/W under 365 nm UV light irradiation,a fast response time of 10/20 ms,outstanding spectra selectivity(UV/visible rejection ratio of 2000),and excellent cycling stability(10,000 cycles),which is one of the best reported PEC UV photodetectors.Furthermore,the self-powered ZnO-based PEC PDs have good underwater optical imaging capability.This work provides a new idea for designing high-performance UV photodetectors for application in underwater environments.
文摘AIM:To evaluate the choroidopathy in patients with systemic lupus erythematosus(SLE)using enhanced depth imaging spectral domain optical coherence tomography(EDI SD-OCT)and optical coherence tomography angiography(OCTA).METHODS:A total of 74 patients with SLE and 40 healthy volunteers were included in this cross-sectional study.SLE patients were further divided into three subgroups based on clinical and blood biochemistry findings.Ocular parameters obtained on ophthalmologic examination and optical imaging(EDI SD-OCT and OCTA)included the best corrected distance visual acuity(logMAR CDVA),subfoveal choroidal thickness(SCT),choroidal vascularity index(CVI)and vessel density(VD)of superficial capillary plexus(SCP)and deep capillary plexus(DCP).RESULTS:SLE patients had significantly lower values for CVI and VD of DCP(DVD)than control subjects.Amongst SLE patients,gender and chloroquine dose were found to be independent determinants of CVI while age predicted SCT.Steroid dose was a significant predictor for foveal VD of SCP(SVD),chloroquine dose for parafoveal SVD,gender for total DVD,and gender and steroid dose for perifoveal DVD.No correlation of logMAR CDVA and SCT was noted between SLE patients and control subjects.No correlation of SCT was noted with disease duration,Systemic Lupus Erythematosus Disease Activity Index(SLEDAI)score,hydroxychloroquine(HCQ)dose or steroid dose.No correlation of CVI was noted with patient age,disease duration,SLEDAI score,HCQ dose or steroid dose.No significant difference was noted between SLE subgroups in terms of any of the ocular parameters studied.CONCLUSION:The findings reveal the presence of ocular findings suggestive of early onset choroidopathy on EDI SD-OCT and OCTA in SLE patients,in the absence of ocular manifestations or active disease.
