Objective: To study the antibacterial and antifungal activities of methanolic extract of roots of Carissa opaca and its fractions in hexane, chloroform, ethyl acetate, n-butanol and water, and the isolated compounds.M...Objective: To study the antibacterial and antifungal activities of methanolic extract of roots of Carissa opaca and its fractions in hexane, chloroform, ethyl acetate, n-butanol and water, and the isolated compounds.Methods: The zones of inhibition of the samples against test microorganisms were determined by agar well diffusion method. Minimum inhibitory concentrations of the samples were determined by agar well dilution method. Test microorganisms included four standard bacteria [Bacillus subtilis ATCC 6633(B. subtilis), Escherichia coli ATCC 8739(E. coli), Pseudomonas aeruginosa ATCC 9027(P. aeruginosa), and Staphylococcus aureus ATCC 6538], two standard fungi [Candida albicans ATCC 10231(C. albicans)] and Aspergillus niger, and six clinical isolates(B. subtilis, E. coli, P. aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterobacter cloacae). The most active fraction was investigated to isolate compounds. The chemical compounds isolated from the ethyl acetate fraction were identified by gas chromatography-mass spectrometer, high performance liquid chromatography and liquid chromatography-mass spectrometer.Results: E. coli, P. aeruginosa, and C. albicans were the most susceptible. Less polar fractions exhibited stronger efficacy than polar ones, and ethyl acetate fraction proved to be the most potent. Zones of inhibition of hexane, chloroform and ethyl acetate fractions, and amoxil against C. albicans were 19.96, 22.01, 23.10 and 19.20 mm, respectively. Ethyl acetate faction was the most toxic to all the test microorganisms, with minimum inhibitory concentrations of 8.0, 7.8 and 7.78 μg/m L against P. aeruginosa, C. albicans and B. subtilis, respectively. Isolated compounds, limonene, 2'-hydroxyacetophenone, vanillin, naphthalenone, 2,3,3-trimethyl-2-(3-methylbuta-1,3-dienyl)-6-methylenecyclohexanone, 2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester, β-sitosterol, vitamin E, rutin, quercetin, lupeol, epigallocatechin, showed considerable antimicrobial activities against test microorganisms.Conclusions: The roots of Carissa opaca contain compounds with significant antimicrobial potential.展开更多
The ability for usage of common freshwater charophytes,Chara aculeolata and Nitella opaca in removal of cadmium （Cd）,lead （Pb） and zinc （Zn） from wastewater was examined.C.aculeolata and N.opaca were exposed to ...The ability for usage of common freshwater charophytes,Chara aculeolata and Nitella opaca in removal of cadmium （Cd）,lead （Pb） and zinc （Zn） from wastewater was examined.C.aculeolata and N.opaca were exposed to various concentrations of Cd （0.25 and 0.5 mg/L）,Pb （5 and 10 mg/L） and Zn （5 and 10 mg/L） solutions under hydroponic conditions for 6 days.C.aculeolata was more tolerant of Cd and Pb than N.opaca.The relative growth rate of N.opaca was drastically reduced at high concentrations of Cd and Pb although both were tolerant of Zn.Both macroalgae showed a reduction in chloroplast,chlorophyll and carotenoid content after Cd and Pb exposure,while Zn exposure had little effects.The bioaccumulation of both Cd and Pb was higher in N.opaca （1544.3 μg/g at 0.5 mg/L Cd,21657.0 μg/g at 10 mg/L Pb） whereas higher Zn accumulation was observed in C.aculeolata （6703.5 μg/g at 10 mg/L Zn）.In addition,high bioconcentration factor values （〉 1000） for Cd and Pb were observed in both species.C.aculeolata showed higher percentage of Cd and Pb removal （〉 95%） than N.opaca and seemed to be a better choice for Cd and Pb removal from wastewater due to its tolerance to these metals.展开更多
基金Supported by Higher Education Commission(HEC)of Pakistan as part of the project No.20-1986/R&D/11
文摘Objective: To study the antibacterial and antifungal activities of methanolic extract of roots of Carissa opaca and its fractions in hexane, chloroform, ethyl acetate, n-butanol and water, and the isolated compounds.Methods: The zones of inhibition of the samples against test microorganisms were determined by agar well diffusion method. Minimum inhibitory concentrations of the samples were determined by agar well dilution method. Test microorganisms included four standard bacteria [Bacillus subtilis ATCC 6633(B. subtilis), Escherichia coli ATCC 8739(E. coli), Pseudomonas aeruginosa ATCC 9027(P. aeruginosa), and Staphylococcus aureus ATCC 6538], two standard fungi [Candida albicans ATCC 10231(C. albicans)] and Aspergillus niger, and six clinical isolates(B. subtilis, E. coli, P. aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterobacter cloacae). The most active fraction was investigated to isolate compounds. The chemical compounds isolated from the ethyl acetate fraction were identified by gas chromatography-mass spectrometer, high performance liquid chromatography and liquid chromatography-mass spectrometer.Results: E. coli, P. aeruginosa, and C. albicans were the most susceptible. Less polar fractions exhibited stronger efficacy than polar ones, and ethyl acetate fraction proved to be the most potent. Zones of inhibition of hexane, chloroform and ethyl acetate fractions, and amoxil against C. albicans were 19.96, 22.01, 23.10 and 19.20 mm, respectively. Ethyl acetate faction was the most toxic to all the test microorganisms, with minimum inhibitory concentrations of 8.0, 7.8 and 7.78 μg/m L against P. aeruginosa, C. albicans and B. subtilis, respectively. Isolated compounds, limonene, 2'-hydroxyacetophenone, vanillin, naphthalenone, 2,3,3-trimethyl-2-(3-methylbuta-1,3-dienyl)-6-methylenecyclohexanone, 2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester, β-sitosterol, vitamin E, rutin, quercetin, lupeol, epigallocatechin, showed considerable antimicrobial activities against test microorganisms.Conclusions: The roots of Carissa opaca contain compounds with significant antimicrobial potential.
基金supported by the grants from the Center of Excellence for Environmental Health,Toxicology and Management of Chemicals under Science&Technology Postgraduate Education and Research Development Officethe Royal Golden Jubilee Ph.D.Program under Thailand Research Fund
文摘The ability for usage of common freshwater charophytes,Chara aculeolata and Nitella opaca in removal of cadmium （Cd）,lead （Pb） and zinc （Zn） from wastewater was examined.C.aculeolata and N.opaca were exposed to various concentrations of Cd （0.25 and 0.5 mg/L）,Pb （5 and 10 mg/L） and Zn （5 and 10 mg/L） solutions under hydroponic conditions for 6 days.C.aculeolata was more tolerant of Cd and Pb than N.opaca.The relative growth rate of N.opaca was drastically reduced at high concentrations of Cd and Pb although both were tolerant of Zn.Both macroalgae showed a reduction in chloroplast,chlorophyll and carotenoid content after Cd and Pb exposure,while Zn exposure had little effects.The bioaccumulation of both Cd and Pb was higher in N.opaca （1544.3 μg/g at 0.5 mg/L Cd,21657.0 μg/g at 10 mg/L Pb） whereas higher Zn accumulation was observed in C.aculeolata （6703.5 μg/g at 10 mg/L Zn）.In addition,high bioconcentration factor values （〉 1000） for Cd and Pb were observed in both species.C.aculeolata showed higher percentage of Cd and Pb removal （〉 95%） than N.opaca and seemed to be a better choice for Cd and Pb removal from wastewater due to its tolerance to these metals.
