目的探索无创光疗(non-damage phototherapy,NDPT)不同干预时长对小鼠膝骨关节炎(knee osteoarthritis,KOA)的治疗效果。方法研究对象为Balb/c小鼠54只,随机分为空白对照组、模型对照组和光疗组,每组18只小鼠。其中光疗组和模型对照组...目的探索无创光疗(non-damage phototherapy,NDPT)不同干预时长对小鼠膝骨关节炎(knee osteoarthritis,KOA)的治疗效果。方法研究对象为Balb/c小鼠54只,随机分为空白对照组、模型对照组和光疗组,每组18只小鼠。其中光疗组和模型对照组均在小鼠右膝关节腔内单次注射Ⅶ型胶原酶1U(6μl)建立KOA模型,光疗组在造模后第1天开始行NDPT照射,照射光参数:波长630 nm发光二极管(light emitting diode,LED),功率密度为10 mW/cm2,每日1次,每次1 h,连续照射至造模后12周;模型对照组不进行NDPT照射。空白对照组在相同部位注射等量磷酸盐缓冲溶液(phosphate buffered saline,PBS),也不进行LED照射。造模后4周、8周、12周,三组小鼠行右后肢足疼痛阈值评价,之后每组每个时间点取6只小鼠处死取膝关节进行组织病理学分析包括苏木精伊红(hematoxylin&eosin,HE)染色和番红固绿(saffron O fast green,SF)染色观察滑膜和软骨组织改变。并采用Krenn滑膜炎严重程度评分,对滑膜炎症进行评估;采用国际骨关节炎研究协会(Osteoarthritis Research Society International,OARSI)软骨损伤等级评分,对软骨损伤进行定量评估。免疫组织化学染色对软骨组织Ⅱ型胶原(Col-Ⅱ)阳性细胞比例表达情况半定量分析。结果造模后1 d,光疗组和模型对照组小鼠右侧膝关节出现红肿及触痛反应,说明造模成功;空白对照组右侧膝关节保持正常状态,无红肿及触痛反应。造模后4周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组,差异具有统计学意义(P<0.05);三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次为空白对照组>光疗组>模型对照组(P<0.05)。造模后8周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组,此时光疗组与空白对照组差异无统计学意义(P>0.05),提示光疗组疼痛恢复正常水平;三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照组(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次空白对照组>光疗组>模型对照组(P<0.05),其中光疗组Col-Ⅱ阳性细胞比例与空白对照组比较差异无统计学意义(P>0.05)。造模后12周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组;三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照组,其中滑膜炎评分差异具有统计学意义(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次空白对照组>光疗组>模型对照组,差异具有统计学意义(P<0.05)。结论在小鼠KOA的早期开始进行12周NDPT干预,能够不同程度地缓解疼痛,改善滑膜炎和软骨损伤,延缓疾病进程。展开更多
During the summer of 2012, the fifth CHINARE Arctic Expedition was carried out, and a submersible mooring system was deployed in M5 station located at （69°30.155＇N,169°00.654＇W） and recovered 50d later. ...During the summer of 2012, the fifth CHINARE Arctic Expedition was carried out, and a submersible mooring system was deployed in M5 station located at （69°30.155＇N,169°00.654＇W） and recovered 50d later. A set of temperature, salinity and current profile records was acquired. The characteristics of these observations are analyzed in this paper. Some main results are achieved as below. （1） Temperature generally decreases while salinity generally increases with increasing depth. The average values of all records are 2.98℃ and 32.21 psu. （2） Salinity and temperature are well negatively correlated, and the correlation coefficient between them is -0.84. However, they did not always vary synchronously. Their co-variation featured different characters during different significant periods. （3） The average velocity for the whole water column is 141 mm/s with directional angle of 347.1°. The statistical distribution curve of velocity record number gets narrower with increasing depth. More than 85% of the recorded velocities are northward, and the mean magnitudes of dominated northward velocities are 100-150 mm/s. （4） Rotary spectrum analysis shows that motions with low frequency take a majority of energy in all layers. The most significant energy peaks for all layers are around 0.012 cph （about 3.5 d period）, while the tidal motion in mooring area is nonsignificant. （5） Velocities in all layers feature similar and synchronous temporal variations, except for the slight decrease in magnitude and leftward twist from top to bottom. The directions of velocity correspond well to those of Surface wind. The average northward volume transport per square meter is 0.1-0.2 m3/s under southerly wind, but about -0.2 m3/s during northerly wind burst.展开更多
文摘目的探索无创光疗(non-damage phototherapy,NDPT)不同干预时长对小鼠膝骨关节炎(knee osteoarthritis,KOA)的治疗效果。方法研究对象为Balb/c小鼠54只,随机分为空白对照组、模型对照组和光疗组,每组18只小鼠。其中光疗组和模型对照组均在小鼠右膝关节腔内单次注射Ⅶ型胶原酶1U(6μl)建立KOA模型,光疗组在造模后第1天开始行NDPT照射,照射光参数:波长630 nm发光二极管(light emitting diode,LED),功率密度为10 mW/cm2,每日1次,每次1 h,连续照射至造模后12周;模型对照组不进行NDPT照射。空白对照组在相同部位注射等量磷酸盐缓冲溶液(phosphate buffered saline,PBS),也不进行LED照射。造模后4周、8周、12周,三组小鼠行右后肢足疼痛阈值评价,之后每组每个时间点取6只小鼠处死取膝关节进行组织病理学分析包括苏木精伊红(hematoxylin&eosin,HE)染色和番红固绿(saffron O fast green,SF)染色观察滑膜和软骨组织改变。并采用Krenn滑膜炎严重程度评分,对滑膜炎症进行评估;采用国际骨关节炎研究协会(Osteoarthritis Research Society International,OARSI)软骨损伤等级评分,对软骨损伤进行定量评估。免疫组织化学染色对软骨组织Ⅱ型胶原(Col-Ⅱ)阳性细胞比例表达情况半定量分析。结果造模后1 d,光疗组和模型对照组小鼠右侧膝关节出现红肿及触痛反应,说明造模成功;空白对照组右侧膝关节保持正常状态,无红肿及触痛反应。造模后4周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组,差异具有统计学意义(P<0.05);三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次为空白对照组>光疗组>模型对照组(P<0.05)。造模后8周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组,此时光疗组与空白对照组差异无统计学意义(P>0.05),提示光疗组疼痛恢复正常水平;三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照组(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次空白对照组>光疗组>模型对照组(P<0.05),其中光疗组Col-Ⅱ阳性细胞比例与空白对照组比较差异无统计学意义(P>0.05)。造模后12周,三组机械触痛阈值依次为空白对照组>光疗组>模型对照组;三组滑膜炎和软骨损伤评分大小依次均为模型对照组>光疗组>空白对照组,其中滑膜炎评分差异具有统计学意义(P<0.05);三组小鼠软骨组织Col-Ⅱ阳性细胞表达量依次空白对照组>光疗组>模型对照组,差异具有统计学意义(P<0.05)。结论在小鼠KOA的早期开始进行12周NDPT干预,能够不同程度地缓解疼痛,改善滑膜炎和软骨损伤,延缓疾病进程。
基金Chinese Polar Environment Comprehensive Investigation and Assessment Programmes,State Oceanic Administration under contract Nos CHINARE2014-03-01 and CHINARE2014-04-03the Public Science and Technology Research Funds Projects of Ocean under contract No.201205007-1the Basic Research Fund under contract No.GY02-2007T08
文摘During the summer of 2012, the fifth CHINARE Arctic Expedition was carried out, and a submersible mooring system was deployed in M5 station located at （69°30.155＇N,169°00.654＇W） and recovered 50d later. A set of temperature, salinity and current profile records was acquired. The characteristics of these observations are analyzed in this paper. Some main results are achieved as below. （1） Temperature generally decreases while salinity generally increases with increasing depth. The average values of all records are 2.98℃ and 32.21 psu. （2） Salinity and temperature are well negatively correlated, and the correlation coefficient between them is -0.84. However, they did not always vary synchronously. Their co-variation featured different characters during different significant periods. （3） The average velocity for the whole water column is 141 mm/s with directional angle of 347.1°. The statistical distribution curve of velocity record number gets narrower with increasing depth. More than 85% of the recorded velocities are northward, and the mean magnitudes of dominated northward velocities are 100-150 mm/s. （4） Rotary spectrum analysis shows that motions with low frequency take a majority of energy in all layers. The most significant energy peaks for all layers are around 0.012 cph （about 3.5 d period）, while the tidal motion in mooring area is nonsignificant. （5） Velocities in all layers feature similar and synchronous temporal variations, except for the slight decrease in magnitude and leftward twist from top to bottom. The directions of velocity correspond well to those of Surface wind. The average northward volume transport per square meter is 0.1-0.2 m3/s under southerly wind, but about -0.2 m3/s during northerly wind burst.
