BACKGROUND Inflammatory bowel disease(IBD)is a common chronic intestinal inflammatory disease.High oxidative stress is a treatment target for IBD.Cerium oxide(CeO2)nanomaterials as nanozymes with antioxidant activity ...BACKGROUND Inflammatory bowel disease(IBD)is a common chronic intestinal inflammatory disease.High oxidative stress is a treatment target for IBD.Cerium oxide(CeO2)nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of colitis.AIM To synthesize hollow cerium(H-CeO2)nanoparticles by one-step method and to validate the therapeutic efficacy of H-CeO2 in IBD.METHODS H-CeO2 was synthesized by one-step method and examined its characterization and nanoenzymatic activity.Subsequently,we constructed dextran sulfate so-dium(DSS)-induced colitis in mice to observe the effects of H-CeO2 on colonic inflammation.The effects of H-CeO2 on colon inflammation and reactive oxygen species(ROS)levels in IBD mice were detected by hematoxylin and eosin staining and dichlorofluorescein diacetate staining,respectively.Finally,the biological sa-fety of H-CeO2 on mice was evaluated by hematoxylin and eosin staining,blood routine,and blood biochemistry.RESULTS H-CeO2 nanoparticles prepared by the one-step method were uniform,monodi-sperse and hollow.H-CeO2 had a good ability to scavenge ROS,∙OH and∙OOH.H-CeO2 reduced DSS-induced decreases in body weight and colon length,colonic epithelial damage,inflammatory infiltration,and ROS accumulation.H-CeO2 administration reduced the disease activity index of DSS-induced animals from about 8 to 5.H-CeO2 had no significant effect on body weight,total platelet count,hemoglobin,white blood cell,and red blood cell counts in healthy mice.No significant damage to major organs was observed in healthy mice following H-CeO2 administration.CONCLUSION The one-step synthesis of H-CeO2 nanomaterials had good antioxidant activity,biosafety,and inhibited deve-lopment of DSS-induced IBD in mice by scavenging ROS.展开更多
The three-dimensional(3D) Pd-based nanoflower structures,assembled from two-dimensional(2D)nanosheets,are characterized by their stable and ordered configurations.These structures have been extensively designed as ano...The three-dimensional(3D) Pd-based nanoflower structures,assembled from two-dimensional(2D)nanosheets,are characterized by their stable and ordered configurations.These structures have been extensively designed as anode materials for fuel cells.However,the exploration of trimetallic nanoflowers with porous architectures remains limited.In this study,we present a straightforward one-step solvothermal method for the synthesis of trimetallic Pd Cu Ni porous nanoflowers(PNFs).Leveraging several unique advantages,such as an open superstructure,high porosity,and enhanced electronic interactions among the trimetals,the resulting Pd Cu Ni PNFs demonstrate significantly improved electrochemical performance,with mass activities reaching 5.94 and 10.14 A/mg for the ethanol oxidation reaction(EOR)and the ethylene glycol oxidation reaction(EGOR),respectively.Furthermore,the Pd Cu Ni PNFs exhibit optimized d-band centers and the most negative onset oxidation potential,indicating enhanced antitoxicity and stability.This study not only provides a novel perspective on the synthesis of 3D porous nanomaterials but also highlights the potential application value of trimetallic nanoalloys in catalysis.展开更多
Gradiently denitrated gun propellant(GDGP)prepared by a“gradient denitration”strategy is obviously superior in progressive burning performance to the traditional deterred gun propellant.Currently,the preparation of ...Gradiently denitrated gun propellant(GDGP)prepared by a“gradient denitration”strategy is obviously superior in progressive burning performance to the traditional deterred gun propellant.Currently,the preparation of GDGP employed a tedious two-step method involving organic solvents,which hinders the large-scale preparation of GDGP.In this paper,GDGP was successfully prepared via a novelty and environmentally friendly one-step method.The obtained samples were characterized by FT-IR,Raman,SEM and XPS.The results showed that the content of nitrate groups gradiently increased from the surface to the core in the surface layer of GDGP and the surface layer of GDGP exhibited a higher compaction than that of raw gun propellant,with a well-preserved nitrocellulose structure.The denitration process enabled the propellant surface with regressive energy density and good progressive burning performance,as confirmed by oxygen bomb and closed bomb test.At the same time,the effects of different solvents on the component loss of propellant were compared.The result showed that water caused the least component loss.Finally,the stability of GDGP was confirmed by methyl-violet test.This work not only provided environmentally friendly,simple and economic preparation of GDGP,but also confirmed the stability of GDGP prepared by this method.展开更多
In this paper, a new computational method for improving the accuracy of numerically computed solutions is introduced. The computational method is based on the one-step method and conserved quantities of holonomic syst...In this paper, a new computational method for improving the accuracy of numerically computed solutions is introduced. The computational method is based on the one-step method and conserved quantities of holonomic systems are considered as kinematical constraints in this method.展开更多
A computational method of constraint stabilization and correction is introduced. The method is based on the Baumgart＇s one-step method. Constraint conditions are addressed to stabilize and correct the solution. Two e...A computational method of constraint stabilization and correction is introduced. The method is based on the Baumgart＇s one-step method. Constraint conditions are addressed to stabilize and correct the solution. Two examples are given to illustrate the results of the method.展开更多
Metal clusters RCCo_3(CO)_9(R-H,C1,Br,CH_3,Ph) were prepared in 18.8-57.3% yields from the reaction of cobalt(Ⅱ)salt and RCX_a under mild PTC conditions(latm CO,25℃).The cobalt salt was reduced to Co(CO)_4 in the pr...Metal clusters RCCo_3(CO)_9(R-H,C1,Br,CH_3,Ph) were prepared in 18.8-57.3% yields from the reaction of cobalt(Ⅱ)salt and RCX_a under mild PTC conditions(latm CO,25℃).The cobalt salt was reduced to Co(CO)_4 in the presence of Na_3S_2O_4.展开更多
Cd S/Mo S2,an extremely efficient photocatalyst,has been extensively used in hydrogen photoproduction and pollutant degradation.Cd S/Mo S2can be synthesized by a facile one-step hydrothermal process.However,the effect...Cd S/Mo S2,an extremely efficient photocatalyst,has been extensively used in hydrogen photoproduction and pollutant degradation.Cd S/Mo S2can be synthesized by a facile one-step hydrothermal process.However,the effect of the sulfur source on the synthesis of Cd S/Mo S2via one-step hydrothermal methods has seldom been investigated.We report herein a series of one-step hydrothermal preparations of Cd S/Mo S2using three different sulfur sources：thioacetamide,L-cysteine,and thiourea.The results revealed that the sulfur source strongly affected the crystallization,morphology,elemental composition and ultraviolet（UV）–visible-light-absorption ability of the Cd S/Mo S2.Among the investigated sulfur sources,thioacetamide provided the highest visible-light absorption ability for Cd S/Mo S2,with the smallest average particle size and largest surface area,resulting in the highest efficiency in Methylene Blue（MB）degradation.The photocatalytic activity of Cd S/Mo S2synthesized from the three sulfur sources can be arranged in the following order：thioacetamideL-cysteinethiourea.The reaction rate constants（k）for thioacetamide,L-cysteine,and thiourea were estimated to be 0.0197,0.0140,and 0.0084 min^（-1）,respectively.However,thioacetamide may be limited in practical application in terms of its price and toxicity,while L-cysteine is relatively economical,less toxic and exhibited good photocatalytic degradation performance toward MB.展开更多
According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleoti...According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.展开更多
Recently, the incidence of<span> </span><i><span>Candida</span></i><span> infections has substantially increased. Conventional identification methods for </span><...Recently, the incidence of<span> </span><i><span>Candida</span></i><span> infections has substantially increased. Conventional identification methods for </span><i><span>Candida</span></i><span> species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously</span><span> </span><span>eight medically important </span><i><span>Candida</span></i><span> species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important </span><i><span>Candida</span></i><span> species. These primers were able to distinguish each </span><i><span>Candida</span></i><span> species and did not display cross-reactivity with representative </span><i><span>Candida </span></i><span>species other than the eight</span><i><span> Candida</span></i><span> species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.</span>展开更多
The bulk metal forming processes were simulated by using a one-step finite element(FE)approach based on deformation theory of plasticity,which enables rapid prediction of final workpiece configurations and stress/stra...The bulk metal forming processes were simulated by using a one-step finite element(FE)approach based on deformation theory of plasticity,which enables rapid prediction of final workpiece configurations and stress/strain distributions.This approach was implemented to minimize the approximated plastic potential energy derived from the total plastic work and the equivalent external work in static equilibrium,for incompressibly rigid-plastic materials,by FE calculation based on the extremum work principle.The one-step forward simulations of compression and rolling processes were presented as examples,and the results were compared with those obtained by classical incremental FE simulation to verify the feasibility and validity of the proposed method.展开更多
For the traditional one-step formulations of using shell elements, the computations of the curvature variation and bending stiffness matrix were simplified by omitting the rotational DOFs （degrees of freedom） on the...For the traditional one-step formulations of using shell elements, the computations of the curvature variation and bending stiffness matrix were simplified by omitting the rotational DOFs （degrees of freedom） on the basis of initial flat blank and fully known final configuration. They were highly efficient but not suitable either for the forming processes with non-flat initial configurations or for one-step forward and multistep analyses. Thus, a one-step formulation based on the rotation-free BST （Basic Shell Triangle） element was presented. In this formulation, the penalty method was adopted to deal with contacts in the forming processes.展开更多
The work is dedicated to develop a one-step eco-friendly method to prepare antibacterial polyethylene terephthalate(PET).We report a one-step eco-friendly method to manufacture antibacterial PET via on-line amination ...The work is dedicated to develop a one-step eco-friendly method to prepare antibacterial polyethylene terephthalate(PET).We report a one-step eco-friendly method to manufacture antibacterial PET via on-line amination reaction by melt coextrusion.Beside evenly mixing of poly(hexamethylene guanidine)(PHMG)and PET in the melt coextrusion procedure,the amination reaction also occurred between PHMG and PET under high temperature(230-270℃).The antibacterial ability of composite PET showed obvious PHMG concentration dependence,and antibacterial activity reached more than 99%when PHMG content was 2.5 wt%.Moreover,LIVE/DEAD fluorescence test further confirmed that the composite PET could kill bacteria quickly and efiectively(within 30 min);while negligible cytotoxicity was observed to HSF and HUVEC cells.Onestep eco-friendly fabrication of composite antibacterial PET was accomplished by on-line melt coextrusion.The composite antibacterial PET has potential use in multiple fields to combat with pathogenic including textiles,packaging materials,decoration materials and biomedical devices,etc.展开更多
We study the strong convergence of the general one-step method for neutral stochastic delay differential equations with a variable delay.First,we give the notions of C-stability and B-consistency,and then establish a ...We study the strong convergence of the general one-step method for neutral stochastic delay differential equations with a variable delay.First,we give the notions of C-stability and B-consistency,and then establish a fundamental theorem of strong convergence for the general one-step method solving the nonlinear neutral stochastic delay differential equations,where the corresponding diffusion coefficient with respect to the non-delay variables is highly nonlinear.Then,we construct the split-step backward Euler method which is a special implicit one-step method,and prove that it is C-stable,B-consistent,and strongly convergent of order 1/2.Finally,we give some numerical experiments to support the obtained results.展开更多
The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in i...The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in improving physiochemical and biological properties of native collagen sponge （Control group）. Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.展开更多
In this study, different methods were used to prepare MoO3/ZrO2 catalysts for sulfur resistant methanation reaction. It was found that MoO3/ZrO2 catalyst prepared by one-step co-precipitation method achieved high meth...In this study, different methods were used to prepare MoO3/ZrO2 catalysts for sulfur resistant methanation reaction. It was found that MoO3/ZrO2 catalyst prepared by one-step co-precipitation method achieved high methanation performance. CO conversion could reach up to 90% on 25 wt% MoO3/ZrO2 catalyst, much higher than that on the conventional 25 wt% MoO3/Al2O3 catalyst. The Mo-based catalysts were characterized by XRF, XRD, Raman, BET, TEM and H2-TPR etc. It was found that MoO3 particles were highly dispersed on ZrO2 support for 25 wt% MoO3/ZrO2 catalyst prepared at 65-85℃ because of its relatively larger pore size, which contributed to a high CO conversion. Meanwhile, when MoO3 loading exceeded the monolayer coverage, the formed crystalline MoO3 and ZrM020g might block the micropores of the catalyst and make the methanation activity declined. These results are useful for preparing highly efficient catalyst for CO methanation process.展开更多
In this article,a procedure for estimating the coefficient functions on the functional-coefficient regression models with different smoothing variables in different coefficient functions is defined.First step,by the l...In this article,a procedure for estimating the coefficient functions on the functional-coefficient regression models with different smoothing variables in different coefficient functions is defined.First step,by the local linear technique and the averaged method,the initial estimates of the coefficient functions are given.Second step,based on the initial estimates,the efficient estimates of the coefficient functions are proposed by a one-step back-fitting procedure.The efficient estimators share the same asymptotic normalities as the local linear estimators for the functional-coefficient models with a single smoothing variable in different functions.Two simulated examples show that the procedure is effective.展开更多
A noninterior continuation method is presented, with only the certering step used at each iteration, for nonlinear complementarity problem. It is shown that the algorithm is globally linearly and locally quadratically...A noninterior continuation method is presented, with only the certering step used at each iteration, for nonlinear complementarity problem. It is shown that the algorithm is globally linearly and locally quadratically convergent under certain conditions.展开更多
This short communication reports our recent work on the synthesis and characterisation ofmicrocapsules of phase change materials using silica as the shell material through a one-step method. The method uses no surfact...This short communication reports our recent work on the synthesis and characterisation ofmicrocapsules of phase change materials using silica as the shell material through a one-step method. The method uses no surfactants or dispersants for stabilising the capsules. The results show that the one-step method allows the tuning of the size and polydispersity of the capsules, and the use of different core materials. Analyses of the capsules show that they contain about 65% phase change materials. The results also suggest no need for a stabilising agent due to self-stabilisation by the amine groups. Further work is underway to investigate the mechanical and thermal properties of the microcapsules and the scale-up of the method.展开更多
An efficient and convenient one-step process was developed for synthesizing new effective red luminous materials through ordered mesoporous γ-alumina assembling with Eu3＋. Employing P123 as a structure-directing age...An efficient and convenient one-step process was developed for synthesizing new effective red luminous materials through ordered mesoporous γ-alumina assembling with Eu3＋. Employing P123 as a structure-directing agent and hydrochloric acid, citric acid as pH adjustor, ordered mesoporous γ-alumina was fabricated by simple sol-gel method. The pore structure was characterized by X-ray diffraction （XRD）, N2 adsorption-desorption isotherms and transmission electron microscopy （TEM）. The as-synthesized γ-aluminas had narrow pore-size distribution （5–7 nm）, large surface area （246 m2/g） and high thermal stability （750–1000 oC）. The luminous property of materials was characterized by Photoluminescence （PL） spectra. The γ-Al2O3：Eu3＋ materials had efficient luminescence, and the emission strength was related to the content of Eu3＋.展开更多
Investigations of two-dimensional(2D)/one-dimensional(1D)van der Waals(vdW)heterojunctions have attracted significant attention due to their excellent properties such as the smooth heterointerface,the highly gate-tuna...Investigations of two-dimensional(2D)/one-dimensional(1D)van der Waals(vdW)heterojunctions have attracted significant attention due to their excellent properties such as the smooth heterointerface,the highly gate-tunable bandgap,and the ultrafast carrier transport.However,the complicated method of manufacturing vdW heterojunction represents a major problem that severely limits their practical applications.Herein,we develop one-step hydrothermal method and use it to synthesize 2D PbI_(2)/1D Pb_(5)S_(2)I_(6)vdW heterojunction.The PbI_(2)/Pb_(5)S_(2)I_(6)vdW heterojunction photodetector(PD)displays lower dark current(<20 pA),higher responsivity(up to 134 mA·W-1),self-powered and wider response spectrum in comparison with that of pristine PbI_(2)PD and Pb_(5)S_(2)I_(6)PD.This one-step hydrothermal method provides a new idea for preparing other mixed-dimensional heterojunction.展开更多
文摘BACKGROUND Inflammatory bowel disease(IBD)is a common chronic intestinal inflammatory disease.High oxidative stress is a treatment target for IBD.Cerium oxide(CeO2)nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of colitis.AIM To synthesize hollow cerium(H-CeO2)nanoparticles by one-step method and to validate the therapeutic efficacy of H-CeO2 in IBD.METHODS H-CeO2 was synthesized by one-step method and examined its characterization and nanoenzymatic activity.Subsequently,we constructed dextran sulfate so-dium(DSS)-induced colitis in mice to observe the effects of H-CeO2 on colonic inflammation.The effects of H-CeO2 on colon inflammation and reactive oxygen species(ROS)levels in IBD mice were detected by hematoxylin and eosin staining and dichlorofluorescein diacetate staining,respectively.Finally,the biological sa-fety of H-CeO2 on mice was evaluated by hematoxylin and eosin staining,blood routine,and blood biochemistry.RESULTS H-CeO2 nanoparticles prepared by the one-step method were uniform,monodi-sperse and hollow.H-CeO2 had a good ability to scavenge ROS,∙OH and∙OOH.H-CeO2 reduced DSS-induced decreases in body weight and colon length,colonic epithelial damage,inflammatory infiltration,and ROS accumulation.H-CeO2 administration reduced the disease activity index of DSS-induced animals from about 8 to 5.H-CeO2 had no significant effect on body weight,total platelet count,hemoglobin,white blood cell,and red blood cell counts in healthy mice.No significant damage to major organs was observed in healthy mice following H-CeO2 administration.CONCLUSION The one-step synthesis of H-CeO2 nanomaterials had good antioxidant activity,biosafety,and inhibited deve-lopment of DSS-induced IBD in mice by scavenging ROS.
基金supported by the National Natural Science Foundation of China (No.52274304)。
文摘The three-dimensional(3D) Pd-based nanoflower structures,assembled from two-dimensional(2D)nanosheets,are characterized by their stable and ordered configurations.These structures have been extensively designed as anode materials for fuel cells.However,the exploration of trimetallic nanoflowers with porous architectures remains limited.In this study,we present a straightforward one-step solvothermal method for the synthesis of trimetallic Pd Cu Ni porous nanoflowers(PNFs).Leveraging several unique advantages,such as an open superstructure,high porosity,and enhanced electronic interactions among the trimetals,the resulting Pd Cu Ni PNFs demonstrate significantly improved electrochemical performance,with mass activities reaching 5.94 and 10.14 A/mg for the ethanol oxidation reaction(EOR)and the ethylene glycol oxidation reaction(EGOR),respectively.Furthermore,the Pd Cu Ni PNFs exhibit optimized d-band centers and the most negative onset oxidation potential,indicating enhanced antitoxicity and stability.This study not only provides a novel perspective on the synthesis of 3D porous nanomaterials but also highlights the potential application value of trimetallic nanoalloys in catalysis.
文摘Gradiently denitrated gun propellant(GDGP)prepared by a“gradient denitration”strategy is obviously superior in progressive burning performance to the traditional deterred gun propellant.Currently,the preparation of GDGP employed a tedious two-step method involving organic solvents,which hinders the large-scale preparation of GDGP.In this paper,GDGP was successfully prepared via a novelty and environmentally friendly one-step method.The obtained samples were characterized by FT-IR,Raman,SEM and XPS.The results showed that the content of nitrate groups gradiently increased from the surface to the core in the surface layer of GDGP and the surface layer of GDGP exhibited a higher compaction than that of raw gun propellant,with a well-preserved nitrocellulose structure.The denitration process enabled the propellant surface with regressive energy density and good progressive burning performance,as confirmed by oxygen bomb and closed bomb test.At the same time,the effects of different solvents on the component loss of propellant were compared.The result showed that water caused the least component loss.Finally,the stability of GDGP was confirmed by methyl-violet test.This work not only provided environmentally friendly,simple and economic preparation of GDGP,but also confirmed the stability of GDGP prepared by this method.
基金Project supported by the National Natural Science Foundation of China （Grant Nos 10572021 and 10372053）, and the Fundamental Research Foundation of Beijing Institute of Technology （BIT-UBF-200507A4206）.
文摘In this paper, a new computational method for improving the accuracy of numerically computed solutions is introduced. The computational method is based on the one-step method and conserved quantities of holonomic systems are considered as kinematical constraints in this method.
基金the National Natural Science Foundation of China (10572021 ,10372053)Basic Research Foundation of Beijing Institute of Tech-nology (BIT-UBF-200507A4206)
文摘A computational method of constraint stabilization and correction is introduced. The method is based on the Baumgart＇s one-step method. Constraint conditions are addressed to stabilize and correct the solution. Two examples are given to illustrate the results of the method.
文摘Metal clusters RCCo_3(CO)_9(R-H,C1,Br,CH_3,Ph) were prepared in 18.8-57.3% yields from the reaction of cobalt(Ⅱ)salt and RCX_a under mild PTC conditions(latm CO,25℃).The cobalt salt was reduced to Co(CO)_4 in the presence of Na_3S_2O_4.
基金supported by the National Natural Science Foundation of China (No. 51478431)a Science and TechnologyPlanning Project from the Science and Technology Department in Zhejiang Province (No. 2014C33028)funding as a Xinmiao Talent Project of Zhejiang Province (No. 2016R408074)
文摘Cd S/Mo S2,an extremely efficient photocatalyst,has been extensively used in hydrogen photoproduction and pollutant degradation.Cd S/Mo S2can be synthesized by a facile one-step hydrothermal process.However,the effect of the sulfur source on the synthesis of Cd S/Mo S2via one-step hydrothermal methods has seldom been investigated.We report herein a series of one-step hydrothermal preparations of Cd S/Mo S2using three different sulfur sources：thioacetamide,L-cysteine,and thiourea.The results revealed that the sulfur source strongly affected the crystallization,morphology,elemental composition and ultraviolet（UV）–visible-light-absorption ability of the Cd S/Mo S2.Among the investigated sulfur sources,thioacetamide provided the highest visible-light absorption ability for Cd S/Mo S2,with the smallest average particle size and largest surface area,resulting in the highest efficiency in Methylene Blue（MB）degradation.The photocatalytic activity of Cd S/Mo S2synthesized from the three sulfur sources can be arranged in the following order：thioacetamideL-cysteinethiourea.The reaction rate constants（k）for thioacetamide,L-cysteine,and thiourea were estimated to be 0.0197,0.0140,and 0.0084 min^（-1）,respectively.However,thioacetamide may be limited in practical application in terms of its price and toxicity,while L-cysteine is relatively economical,less toxic and exhibited good photocatalytic degradation performance toward MB.
文摘According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.
文摘Recently, the incidence of<span> </span><i><span>Candida</span></i><span> infections has substantially increased. Conventional identification methods for </span><i><span>Candida</span></i><span> species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously</span><span> </span><span>eight medically important </span><i><span>Candida</span></i><span> species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important </span><i><span>Candida</span></i><span> species. These primers were able to distinguish each </span><i><span>Candida</span></i><span> species and did not display cross-reactivity with representative </span><i><span>Candida </span></i><span>species other than the eight</span><i><span> Candida</span></i><span> species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.</span>
基金Project(50575143)supported by the National Natural Science Foundation of ChinaProject(20040248005)supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘The bulk metal forming processes were simulated by using a one-step finite element(FE)approach based on deformation theory of plasticity,which enables rapid prediction of final workpiece configurations and stress/strain distributions.This approach was implemented to minimize the approximated plastic potential energy derived from the total plastic work and the equivalent external work in static equilibrium,for incompressibly rigid-plastic materials,by FE calculation based on the extremum work principle.The one-step forward simulations of compression and rolling processes were presented as examples,and the results were compared with those obtained by classical incremental FE simulation to verify the feasibility and validity of the proposed method.
基金Item Sponsored by National Natural Science Foundation of China (50575134 ,50835002,50975174)
文摘For the traditional one-step formulations of using shell elements, the computations of the curvature variation and bending stiffness matrix were simplified by omitting the rotational DOFs （degrees of freedom） on the basis of initial flat blank and fully known final configuration. They were highly efficient but not suitable either for the forming processes with non-flat initial configurations or for one-step forward and multistep analyses. Thus, a one-step formulation based on the rotation-free BST （Basic Shell Triangle） element was presented. In this formulation, the penalty method was adopted to deal with contacts in the forming processes.
基金Funded by the National Natural Science Foundation of China(No.51703169)Key Program of Science and Technology of Jieyang City(No.2019016)Key Research and Development Program of Shandong Province of China(No.2019JZZY010338)。
文摘The work is dedicated to develop a one-step eco-friendly method to prepare antibacterial polyethylene terephthalate(PET).We report a one-step eco-friendly method to manufacture antibacterial PET via on-line amination reaction by melt coextrusion.Beside evenly mixing of poly(hexamethylene guanidine)(PHMG)and PET in the melt coextrusion procedure,the amination reaction also occurred between PHMG and PET under high temperature(230-270℃).The antibacterial ability of composite PET showed obvious PHMG concentration dependence,and antibacterial activity reached more than 99%when PHMG content was 2.5 wt%.Moreover,LIVE/DEAD fluorescence test further confirmed that the composite PET could kill bacteria quickly and efiectively(within 30 min);while negligible cytotoxicity was observed to HSF and HUVEC cells.Onestep eco-friendly fabrication of composite antibacterial PET was accomplished by on-line melt coextrusion.The composite antibacterial PET has potential use in multiple fields to combat with pathogenic including textiles,packaging materials,decoration materials and biomedical devices,etc.
基金supported by the Key Research Program of Higher Education Institutions of Henan Province(No.24B110019)Basic Research Projects of Key Scientific Research Projects Plan in Henan Higher Education Institutions(No.24ZX008)NSF of China(Nos.12171441 and 12301502).
文摘We study the strong convergence of the general one-step method for neutral stochastic delay differential equations with a variable delay.First,we give the notions of C-stability and B-consistency,and then establish a fundamental theorem of strong convergence for the general one-step method solving the nonlinear neutral stochastic delay differential equations,where the corresponding diffusion coefficient with respect to the non-delay variables is highly nonlinear.Then,we construct the split-step backward Euler method which is a special implicit one-step method,and prove that it is C-stable,B-consistent,and strongly convergent of order 1/2.Finally,we give some numerical experiments to support the obtained results.
基金Funded by the National Natural Science Foundation of China (10832012)the Natural Science Foudation of Tianjin city(08JCYBJC03400)
文摘The aim of the present study was to compare one-step method to EDC/NHS crosslinking （EDC/NHS group） and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization （EDC/NHS- Heparin group） in improving physiochemical and biological properties of native collagen sponge （Control group）. Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.
基金supported by the Tianjin Municipal Science and Technology Commission(14JCZDJC37500)
文摘In this study, different methods were used to prepare MoO3/ZrO2 catalysts for sulfur resistant methanation reaction. It was found that MoO3/ZrO2 catalyst prepared by one-step co-precipitation method achieved high methanation performance. CO conversion could reach up to 90% on 25 wt% MoO3/ZrO2 catalyst, much higher than that on the conventional 25 wt% MoO3/Al2O3 catalyst. The Mo-based catalysts were characterized by XRF, XRD, Raman, BET, TEM and H2-TPR etc. It was found that MoO3 particles were highly dispersed on ZrO2 support for 25 wt% MoO3/ZrO2 catalyst prepared at 65-85℃ because of its relatively larger pore size, which contributed to a high CO conversion. Meanwhile, when MoO3 loading exceeded the monolayer coverage, the formed crystalline MoO3 and ZrM020g might block the micropores of the catalyst and make the methanation activity declined. These results are useful for preparing highly efficient catalyst for CO methanation process.
文摘In this article,a procedure for estimating the coefficient functions on the functional-coefficient regression models with different smoothing variables in different coefficient functions is defined.First step,by the local linear technique and the averaged method,the initial estimates of the coefficient functions are given.Second step,based on the initial estimates,the efficient estimates of the coefficient functions are proposed by a one-step back-fitting procedure.The efficient estimators share the same asymptotic normalities as the local linear estimators for the functional-coefficient models with a single smoothing variable in different functions.Two simulated examples show that the procedure is effective.
文摘A noninterior continuation method is presented, with only the certering step used at each iteration, for nonlinear complementarity problem. It is shown that the algorithm is globally linearly and locally quadratically convergent under certain conditions.
基金supported by UK EPSRC under grants EP/F023014/1 and EP/F000464/1a collaborative research fund from the Institute of Process Engineering of Chinese Academy of Sciences
文摘This short communication reports our recent work on the synthesis and characterisation ofmicrocapsules of phase change materials using silica as the shell material through a one-step method. The method uses no surfactants or dispersants for stabilising the capsules. The results show that the one-step method allows the tuning of the size and polydispersity of the capsules, and the use of different core materials. Analyses of the capsules show that they contain about 65% phase change materials. The results also suggest no need for a stabilising agent due to self-stabilisation by the amine groups. Further work is underway to investigate the mechanical and thermal properties of the microcapsules and the scale-up of the method.
基金Project supported by the National Natural Science Foundation of China (21071063, 50872045)
文摘An efficient and convenient one-step process was developed for synthesizing new effective red luminous materials through ordered mesoporous γ-alumina assembling with Eu3＋. Employing P123 as a structure-directing agent and hydrochloric acid, citric acid as pH adjustor, ordered mesoporous γ-alumina was fabricated by simple sol-gel method. The pore structure was characterized by X-ray diffraction （XRD）, N2 adsorption-desorption isotherms and transmission electron microscopy （TEM）. The as-synthesized γ-aluminas had narrow pore-size distribution （5–7 nm）, large surface area （246 m2/g） and high thermal stability （750–1000 oC）. The luminous property of materials was characterized by Photoluminescence （PL） spectra. The γ-Al2O3：Eu3＋ materials had efficient luminescence, and the emission strength was related to the content of Eu3＋.
基金National Natural Science Foundation of China(No.61376017)。
文摘Investigations of two-dimensional(2D)/one-dimensional(1D)van der Waals(vdW)heterojunctions have attracted significant attention due to their excellent properties such as the smooth heterointerface,the highly gate-tunable bandgap,and the ultrafast carrier transport.However,the complicated method of manufacturing vdW heterojunction represents a major problem that severely limits their practical applications.Herein,we develop one-step hydrothermal method and use it to synthesize 2D PbI_(2)/1D Pb_(5)S_(2)I_(6)vdW heterojunction.The PbI_(2)/Pb_(5)S_(2)I_(6)vdW heterojunction photodetector(PD)displays lower dark current(<20 pA),higher responsivity(up to 134 mA·W-1),self-powered and wider response spectrum in comparison with that of pristine PbI_(2)PD and Pb_(5)S_(2)I_(6)PD.This one-step hydrothermal method provides a new idea for preparing other mixed-dimensional heterojunction.
