The present study is on the haematological and hepatic responses of the African catfish fingerlings Clarias gariepinus to sublethal toxicity of industrial effluents(IE)from the environment of Ologe Lagoon,Lagos,Nigeri...The present study is on the haematological and hepatic responses of the African catfish fingerlings Clarias gariepinus to sublethal toxicity of industrial effluents(IE)from the environment of Ologe Lagoon,Lagos,Nigeria.The fish were cultured in five concentrations of industrial effluents:0%(control),5%,15%,25%,and 35%.Trials were carried out in triplicates for twelve(12)weeks.The culture system was a static renewable bioassay and was carried out in the fisheries laboratory of the Lagos State University,Ojo-Lagos.Weekly physico-chemical parameters:Temperature(°C),pH,conductivity(ppm)and dissolved oxygen(DO in mg/L)were measured in each treatment tank.Haematological parameters:packed cell volume(PCV),red blood cells(RBC),white blood cell(WBC),neutrophil and lymphocytes etc.,and hepatological alterations were measured after 12 weeks.The physico-chemical parameters showed that the pH ranged from 7.82±0.25~8.07±0.02.DO ranged from 1.92±0.66~4.43±1.24 mg/L.The conductivity values increased with increase in concentration of I.E.While the temperature difference remained insignificant with mean value range between 26.08±2.14~26.38±2.28.The DO showed significant differences at p<0.05.Though survival was 100%during the sublethal study,haematological results showed that C.gariepinus had PCV ranging from 13.0±1.7~27.7±0.6,RBC ranged from 4.7±0.6~9.1±0.1,and neutrophil ranged from 26.7±4.6~61.0±1.0 amongst others.The highest values of these parameters were obtained in the control and lowest at 35%.While the reverse effects were observed for WBC and lymphocytes,the liver shows normal liver cells in the control(0%),but at higher toxic levels,there were:vacoulation,destruction of the hepatic parenchyma,tissue becoming eosinophilic(i.e.tending towards Carcinogenicity)and severe disruption of the hepatic cord architecture.This study therefore shows that disposal of effluents into the aquatic environment affects the health of fishes by impairing normal hepatic functions and hindering vital physiological processes if exposure continues for a long period of time(sublethal effect).展开更多
To investigate the levels of IL 18 in the bone marrow of both normal subjects a nd patients with hematological diseases and to determine the possible significan ce of IL 18 in pathogenesis of some hematological mali...To investigate the levels of IL 18 in the bone marrow of both normal subjects a nd patients with hematological diseases and to determine the possible significan ce of IL 18 in pathogenesis of some hematological malignancies Methods The IL 18 mRNA levels in the bone marrow of 140 patients with hematological dis eases and 15 normal donors were determined by the semi quantitative reverse tra nscriptase polymerase chain reaction (RT PCR) Immunohistochemical method was used to detect IL 18 protein in 12 patients with acute myeloid leukemia (AML) The possible regulation of IL 18 for proliferation of some leukemia cells was investigated using antisense techniques Results IL 18 mRNA levels were obviously higher in the patients with leukemia or other malignant hematological diseases (OMHD) than in normal donors However, no sign ificant difference was found in the level of transcription between patients with iron deficiency anemia (IDA) and normal controls Immunohistochemical method c onfirmed the presence of IL 18 protein in 10 out of 12 AML cases with positive transcription By 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium b romide (MTT) assay, IL 18 antisense oligodeoxynucleotides (ASON) clearly inhibi ted the growth of J6 1 and HL 60 cells (42% and 12% inhibited, respectively) i n a dose dependant manner Conclusions IL 18 was detected at elevated levels in the bone marrow of patients with some hematological malignancies, and might be involved in the proliferation of certai n leukemic cells in vivo through an autocrine mechanism展开更多
The presence of a pair of Weyl and Dirac points(WP-DP)in topological semimetal states is intriguing and sought after due to the effects associated with chiral topological charges.However,identifying these states in re...The presence of a pair of Weyl and Dirac points(WP-DP)in topological semimetal states is intriguing and sought after due to the effects associated with chiral topological charges.However,identifying these states in real materials poses a significant challenge.In this study,by means of first-principles calculations we predict the coexistence of charge-2 Dirac and charge-2 Weyl phonons at high-symmetry points within a noncentrosymmetric P4_(1)2_(1)2 space group.Furthermore,we propose GeO_(2)as an ideal candidate for realizing these states.Notably,we observe two distinct surface arcs that connect the Dirac and Weyl points across the entire Brillouin zone,which could facilitate their detection in future experimental investigations.This study not only presents a tangible material for experimentalists to explore the topological properties of WP-DP states but also opens up new avenues in the quest for ideal platforms to study chiral particles.展开更多
This study was carried out to assess the level of heavy metals pollution in Ologe Lagoon, Agbara, Lagos, Nigeria. The Lagoon receives effluents from industries in the Agbara Industrial Estate. Cored Soft sediments wer...This study was carried out to assess the level of heavy metals pollution in Ologe Lagoon, Agbara, Lagos, Nigeria. The Lagoon receives effluents from industries in the Agbara Industrial Estate. Cored Soft sediments were retrieved from six random sampling points within the Lagoon and labeled (S1, S2, S3, S4, S5 and S6). Subsamples of the sediments were prepared and analyzed for heavy metal concentration. The result from the heavy metal analysis of the sediments shows that average concentration of Mn, Zn, Cr, Ni, Cr, Ni, Co, Cu and Pb in the samples was 278.1 ppm, 21.7 ppm, 14.2 ppm, 6.6 ppm, 6.4 ppm, 6.4 ppm and 4.4 ppm, respectively. The correlation between the heavy metals varies from 0.71 to 0.98 shows that they have a strongly positive degree of association which suggests that they are from the same source. The geo-accumulation values for Cu in all stations ranged between (1 - 2.8 ppm) and it can be deduced that the sediments are moderately to strongly polluted. Pb varies between 1 and 2.1 suggesting that the sediments are moderately polluted. Zn exhibits strong pollution levels with a range from 2.5 and 3.5 ppm. Co is moderately polluted with 1.5 - 2.4 while Mn shows the highest level of pollution in all stations with Igeo values ranging between 4.1 and 5.8. This study showed that the Lagoon accumulates heavy metals as a result of anthropogenic activities evident in the dumping of municipal, sewage, industrial waste in the environment and domestic activities such as auto mobile transportation and mechanic repair workshop. Based on sediment quality guidelines, the Ologe Lagoon level of pollution ranged from Cu > Pb > Zn > Ni > Co > Mn > Cr > Al with Mn having the highest level of pollution.展开更多
Based on the conclusion of modern scientific analysis: the cobalt-blue pigment applied on Jingdezhen official porcelain during Yuan Dynasty and prior to Xuande reign of Ming Dynasty (1271―1425) contains high iron and...Based on the conclusion of modern scientific analysis: the cobalt-blue pigment applied on Jingdezhen official porcelain during Yuan Dynasty and prior to Xuande reign of Ming Dynasty (1271―1425) contains high iron and low manganese con- tents, and the cobalt during Xuande and after Xuande periods (after 1426) of Ming Dynasty contains high manganese and low iron contents, in combination with the traditional archaeological method, the related historical textual records were in- vestigated and analyzed, the imported cobalt employed on Jingdezhen official porcelain was studied comprehensively, systematically and logically. In fact, 'Su Ma Li Qing' (Samarra-blue) and 'Su Bo Ni Qing' (Sumatra-blue) are definitely different cobalt ores originated in different places, because of their very similar translitera- tions in Chinese, the confusion has appeared in ancient books for more than 400 years, which considered 'Su Ma Li Qing' and 'Su Bo Ni Qing' as the same co- balt-blue pigment. In this article the mistake was corrected, the possible producing places and the approximate eras of the two pigments imported to China and used at Jingdezhen were also suggested.展开更多
文摘The present study is on the haematological and hepatic responses of the African catfish fingerlings Clarias gariepinus to sublethal toxicity of industrial effluents(IE)from the environment of Ologe Lagoon,Lagos,Nigeria.The fish were cultured in five concentrations of industrial effluents:0%(control),5%,15%,25%,and 35%.Trials were carried out in triplicates for twelve(12)weeks.The culture system was a static renewable bioassay and was carried out in the fisheries laboratory of the Lagos State University,Ojo-Lagos.Weekly physico-chemical parameters:Temperature(°C),pH,conductivity(ppm)and dissolved oxygen(DO in mg/L)were measured in each treatment tank.Haematological parameters:packed cell volume(PCV),red blood cells(RBC),white blood cell(WBC),neutrophil and lymphocytes etc.,and hepatological alterations were measured after 12 weeks.The physico-chemical parameters showed that the pH ranged from 7.82±0.25~8.07±0.02.DO ranged from 1.92±0.66~4.43±1.24 mg/L.The conductivity values increased with increase in concentration of I.E.While the temperature difference remained insignificant with mean value range between 26.08±2.14~26.38±2.28.The DO showed significant differences at p<0.05.Though survival was 100%during the sublethal study,haematological results showed that C.gariepinus had PCV ranging from 13.0±1.7~27.7±0.6,RBC ranged from 4.7±0.6~9.1±0.1,and neutrophil ranged from 26.7±4.6~61.0±1.0 amongst others.The highest values of these parameters were obtained in the control and lowest at 35%.While the reverse effects were observed for WBC and lymphocytes,the liver shows normal liver cells in the control(0%),but at higher toxic levels,there were:vacoulation,destruction of the hepatic parenchyma,tissue becoming eosinophilic(i.e.tending towards Carcinogenicity)and severe disruption of the hepatic cord architecture.This study therefore shows that disposal of effluents into the aquatic environment affects the health of fishes by impairing normal hepatic functions and hindering vital physiological processes if exposure continues for a long period of time(sublethal effect).
基金ThisworkwassupportedbytheNationalNaturalSciencesFoundationofChina (No 39980 0 1 4 )
文摘To investigate the levels of IL 18 in the bone marrow of both normal subjects a nd patients with hematological diseases and to determine the possible significan ce of IL 18 in pathogenesis of some hematological malignancies Methods The IL 18 mRNA levels in the bone marrow of 140 patients with hematological dis eases and 15 normal donors were determined by the semi quantitative reverse tra nscriptase polymerase chain reaction (RT PCR) Immunohistochemical method was used to detect IL 18 protein in 12 patients with acute myeloid leukemia (AML) The possible regulation of IL 18 for proliferation of some leukemia cells was investigated using antisense techniques Results IL 18 mRNA levels were obviously higher in the patients with leukemia or other malignant hematological diseases (OMHD) than in normal donors However, no sign ificant difference was found in the level of transcription between patients with iron deficiency anemia (IDA) and normal controls Immunohistochemical method c onfirmed the presence of IL 18 protein in 10 out of 12 AML cases with positive transcription By 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium b romide (MTT) assay, IL 18 antisense oligodeoxynucleotides (ASON) clearly inhibi ted the growth of J6 1 and HL 60 cells (42% and 12% inhibited, respectively) i n a dose dependant manner Conclusions IL 18 was detected at elevated levels in the bone marrow of patients with some hematological malignancies, and might be involved in the proliferation of certai n leukemic cells in vivo through an autocrine mechanism
基金supported by the National Key R&D Program of China(Grant No.2021YFB3501503)the National Natural Science Foundation of China(Grant No.51474202)+2 种基金Network and Information Foundation of CAS(Grant No.CAS-WX2021SF-0102)the Key Project of Chinese Academy of Sciences(Grant No.ZDRW-CN-2021-2-5)J.X.Li also acknowledges the funding from China Postdoctoral Science Foundation(Grant Nos.2022T150660 and 2021M700152).
文摘The presence of a pair of Weyl and Dirac points(WP-DP)in topological semimetal states is intriguing and sought after due to the effects associated with chiral topological charges.However,identifying these states in real materials poses a significant challenge.In this study,by means of first-principles calculations we predict the coexistence of charge-2 Dirac and charge-2 Weyl phonons at high-symmetry points within a noncentrosymmetric P4_(1)2_(1)2 space group.Furthermore,we propose GeO_(2)as an ideal candidate for realizing these states.Notably,we observe two distinct surface arcs that connect the Dirac and Weyl points across the entire Brillouin zone,which could facilitate their detection in future experimental investigations.This study not only presents a tangible material for experimentalists to explore the topological properties of WP-DP states but also opens up new avenues in the quest for ideal platforms to study chiral particles.
文摘This study was carried out to assess the level of heavy metals pollution in Ologe Lagoon, Agbara, Lagos, Nigeria. The Lagoon receives effluents from industries in the Agbara Industrial Estate. Cored Soft sediments were retrieved from six random sampling points within the Lagoon and labeled (S1, S2, S3, S4, S5 and S6). Subsamples of the sediments were prepared and analyzed for heavy metal concentration. The result from the heavy metal analysis of the sediments shows that average concentration of Mn, Zn, Cr, Ni, Cr, Ni, Co, Cu and Pb in the samples was 278.1 ppm, 21.7 ppm, 14.2 ppm, 6.6 ppm, 6.4 ppm, 6.4 ppm and 4.4 ppm, respectively. The correlation between the heavy metals varies from 0.71 to 0.98 shows that they have a strongly positive degree of association which suggests that they are from the same source. The geo-accumulation values for Cu in all stations ranged between (1 - 2.8 ppm) and it can be deduced that the sediments are moderately to strongly polluted. Pb varies between 1 and 2.1 suggesting that the sediments are moderately polluted. Zn exhibits strong pollution levels with a range from 2.5 and 3.5 ppm. Co is moderately polluted with 1.5 - 2.4 while Mn shows the highest level of pollution in all stations with Igeo values ranging between 4.1 and 5.8. This study showed that the Lagoon accumulates heavy metals as a result of anthropogenic activities evident in the dumping of municipal, sewage, industrial waste in the environment and domestic activities such as auto mobile transportation and mechanic repair workshop. Based on sediment quality guidelines, the Ologe Lagoon level of pollution ranged from Cu > Pb > Zn > Ni > Co > Mn > Cr > Al with Mn having the highest level of pollution.
文摘Based on the conclusion of modern scientific analysis: the cobalt-blue pigment applied on Jingdezhen official porcelain during Yuan Dynasty and prior to Xuande reign of Ming Dynasty (1271―1425) contains high iron and low manganese con- tents, and the cobalt during Xuande and after Xuande periods (after 1426) of Ming Dynasty contains high manganese and low iron contents, in combination with the traditional archaeological method, the related historical textual records were in- vestigated and analyzed, the imported cobalt employed on Jingdezhen official porcelain was studied comprehensively, systematically and logically. In fact, 'Su Ma Li Qing' (Samarra-blue) and 'Su Bo Ni Qing' (Sumatra-blue) are definitely different cobalt ores originated in different places, because of their very similar translitera- tions in Chinese, the confusion has appeared in ancient books for more than 400 years, which considered 'Su Ma Li Qing' and 'Su Bo Ni Qing' as the same co- balt-blue pigment. In this article the mistake was corrected, the possible producing places and the approximate eras of the two pigments imported to China and used at Jingdezhen were also suggested.
