Plant polyploidy often occurs in conjunction with higher yield and superior quality.Therefore,obtaining polyploid germplasms is a significant part of breeding.The oil-tea Camellia tree is an important native woody pla...Plant polyploidy often occurs in conjunction with higher yield and superior quality.Therefore,obtaining polyploid germplasms is a significant part of breeding.The oil-tea Camellia tree is an important native woody plant that produces high-quality edible oil and includes many species of Camellia with different ploidies.However,whether higher ploidy levels in oil-tea Camellia trees are related to better traits remains unclear.In this study,the ploidy levels of 30 different oil-tea Camellia strains in three different species in the Sect.Paracamellia were determined by flow cytometry and chromosome preparation,and the phenotypic characteristics and fatty acid compositions of the fruits were examined by field observations and laboratory analyses.The correlations between the ploidy level of oil-tea Camellia and the main traits of the fruit were investigated.Our results showed that 10 Camellia lanceoleosa strains were diploid,10 Camellia meiocarpa strains were tetraploid and 10 Camellia oleifera strains were hexaploid.Hexaploid C.oleifera had larger fruit size and weight,more seeds per fruit,greater seed weight per fruit,higher oil content and greater yield per crown width than tetraploid C.meiocarpa and diploid C.lanceoleosa,but their fruit peel thickness and fresh seed rate were significantly lower,and these traits were significantly correlated with ploidy level.In addition,in terms of fatty acid composition,hexaploid C.oleifera had a higher oleic acid content than tetraploid C.meiocarpa and diploid C.lanceoleosa,but their linoleic acid,linolenic acid and arachidonic acid contents were lower.The contents of palmitic acid,stearic acid and total unsaturated fatty acids were not significantly correlated with ploidy level.In conclusion,certain correlations exist between the main characteristics of oil-tea Camellia fruit and the ploidy level,and increasing the ploidy level led to an increase in fruit yield with no effect on oil composition.The discovery of variations in the main characteristics of oil-tea Camellia fruit with different ploidies will facilitate germplasm innovation and lay a foundation for ploidy breeding and mechanistic research on fruit traits.展开更多
To determine the age of oil-tea camellia trees, regression equations including Logistic, Mitscherlich, Gompertz, Korf, and Richards were used to calculate accumulative growth rate using basal trunk disc and investigat...To determine the age of oil-tea camellia trees, regression equations including Logistic, Mitscherlich, Gompertz, Korf, and Richards were used to calculate accumulative growth rate using basal trunk disc and investigate the relations between the age of oil-tea camellia trees and their growth rate of secondary trunk. The Gompertz equation Y=71.296 1exp （-3.874 4exp （-0.006 4t）） was the most optimal equation to simulate the accumulative growth rate of basal trunk disc. This equation could be used to estimate the age of oil-tea camellia trees that grow under similar environmental conditions. The Korf equation Y=576.900 1exp （-4.153 0x -0.314 2 ） was the best equation to describe the relation between the age and growth rate of different secondary trunks. With the adjustment coefficient and average growth of different secondary trunk discs, it is possible to predict the age of ancient oil-tea camellia trees that grow under similar environmental conditions. In addition, taking three or more discs from the same diameter group and calculating their average growth rate could lead to more accurate results. For trees that grow in different areas, environmental conditions should be carefully considered when using the above two equations to predict the age of ancient oil-tea camellia trees.展开更多
Oil-tea camellia tree is an important oil plant in China that has long flexible branches.The most challenging feature for the mechanized harvest of oil-tea fruits is that its flower and fruit grow synchronously.In ord...Oil-tea camellia tree is an important oil plant in China that has long flexible branches.The most challenging feature for the mechanized harvest of oil-tea fruits is that its flower and fruit grow synchronously.In order to improve the harvesting efficiency and avoid damaging the flower bud,a hand-held fruit harvesting machine with a variable spacing comb brush was proposed.The harvesting machine can generate three kinds of actuation to detach fruit when it runs.The main actuation results from the brushing of multiple comb fingers.The other two kinds of actuation result from the beating of comb fingers on the fruits and the branches.The finger spacing of the comb brush can be adjusted consequently through moving the spacing adjusting crossbar.Hence,when the finger spacing is smaller than the diameter of the oil-tea fruit,the fruit is brushed off,but the flower bud and leaf pass through the finger gap.When the finger spacing is bigger than the fruit diameter,the fruit stuck between the fingers is loosened to ensure the continuous operation of the machine.Nylon was used as the material of the brush finger to avoid damage,which can also reduce the overall weight.The dynamic simulation of the harvesting machine was carried out with ADAMS,and the acceleration of the front end of the comb finger and the variation of the finger spacing were analyzed.The prototype of the harvesting machine was built and tested in the field.Field experiment results showed that when the speed of the comb finger drive shaft was 480 r/min,the average harvesting percentage of oil-tea fruit was 80%,and the flower bud was seldom detached,which met the working requirements of oil-tea fruit harvesting.展开更多
Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showe...Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showed that 493 discernible loci with distinct elec- trophoretic bands were obtained, of which, 478 loci （96.78%） were polymorphic. This indicated that oil-tea germplasms possess abundant genetic diversities. By clustering analysis performed using softwares of NTSYS 2.10 and Winboot, 51 oil-tea germplasms were divided into two groups： Group I had 48 lines of Camellia oleifera Abel, while Group II had three C. oleifera Abel related species and their similarity coefficient was 0.62. Group I was further divided into Group I-1 and Group I-2, and their similarity coefficient （Gs） was 0.634. All members of Group I-1 originated from Hunan Province, while Group I-2 included the rest of Hunan lines and those originated from other regions of China. Analyzed by software POPGENE 1.32, the Shannon＇s information index （I＊） of genetic polymorphism was 0.3852, the genetic diversity among different region popula- tions （Ht） was 0.2537, the genetic diversity within populations （Hs） was 0.15545, the dif- ferentiation coefficient of genetic diversity among populations （Gst） was 0.3967, and the gene flow among populations （Nm＊） was 0.8262. The Nei＇s genetic distances between the Hunan population and the populations originated from other regions of China implied that geographic isolation strongly influenced genetic differentiation among populations. Meanwhile, seedling rootstock grafting and high grafting for tree crown produced genetic variations among clonal offsprings.展开更多
基金supported by the Special Funds for Construction of Innovative Provinces in Hunan Province,China(2021NK1007)the Hunan Provincial Innovation Foundation for Postgraduate,China(CX20230779)the Scientific Innovation Fund for Post-graduates of Central South University of Forestry and Technology,China(2023CX01009)。
文摘Plant polyploidy often occurs in conjunction with higher yield and superior quality.Therefore,obtaining polyploid germplasms is a significant part of breeding.The oil-tea Camellia tree is an important native woody plant that produces high-quality edible oil and includes many species of Camellia with different ploidies.However,whether higher ploidy levels in oil-tea Camellia trees are related to better traits remains unclear.In this study,the ploidy levels of 30 different oil-tea Camellia strains in three different species in the Sect.Paracamellia were determined by flow cytometry and chromosome preparation,and the phenotypic characteristics and fatty acid compositions of the fruits were examined by field observations and laboratory analyses.The correlations between the ploidy level of oil-tea Camellia and the main traits of the fruit were investigated.Our results showed that 10 Camellia lanceoleosa strains were diploid,10 Camellia meiocarpa strains were tetraploid and 10 Camellia oleifera strains were hexaploid.Hexaploid C.oleifera had larger fruit size and weight,more seeds per fruit,greater seed weight per fruit,higher oil content and greater yield per crown width than tetraploid C.meiocarpa and diploid C.lanceoleosa,but their fruit peel thickness and fresh seed rate were significantly lower,and these traits were significantly correlated with ploidy level.In addition,in terms of fatty acid composition,hexaploid C.oleifera had a higher oleic acid content than tetraploid C.meiocarpa and diploid C.lanceoleosa,but their linoleic acid,linolenic acid and arachidonic acid contents were lower.The contents of palmitic acid,stearic acid and total unsaturated fatty acids were not significantly correlated with ploidy level.In conclusion,certain correlations exist between the main characteristics of oil-tea Camellia fruit and the ploidy level,and increasing the ploidy level led to an increase in fruit yield with no effect on oil composition.The discovery of variations in the main characteristics of oil-tea Camellia fruit with different ploidies will facilitate germplasm innovation and lay a foundation for ploidy breeding and mechanistic research on fruit traits.
基金Supported by Hunan Forestry Science and Technology Project(XLK201707)
文摘To determine the age of oil-tea camellia trees, regression equations including Logistic, Mitscherlich, Gompertz, Korf, and Richards were used to calculate accumulative growth rate using basal trunk disc and investigate the relations between the age of oil-tea camellia trees and their growth rate of secondary trunk. The Gompertz equation Y=71.296 1exp （-3.874 4exp （-0.006 4t）） was the most optimal equation to simulate the accumulative growth rate of basal trunk disc. This equation could be used to estimate the age of oil-tea camellia trees that grow under similar environmental conditions. The Korf equation Y=576.900 1exp （-4.153 0x -0.314 2 ） was the best equation to describe the relation between the age and growth rate of different secondary trunks. With the adjustment coefficient and average growth of different secondary trunk discs, it is possible to predict the age of ancient oil-tea camellia trees that grow under similar environmental conditions. In addition, taking three or more discs from the same diameter group and calculating their average growth rate could lead to more accurate results. For trees that grow in different areas, environmental conditions should be carefully considered when using the above two equations to predict the age of ancient oil-tea camellia trees.
基金This work was supported by the Zhejiang Provincial Key Research&Development Plan(Grant No.2019C02065)the National Natural Science Foundation of China(Grant No.31971798)+1 种基金the National Key Research and Development Program of China(Grant No.2019YFD1001602)the 521 Talent Plan of Zhejiang Sci-Tech University,and the Cultivation Project for Youth Discipline Leader in Zhejiang Provincial Institute.
文摘Oil-tea camellia tree is an important oil plant in China that has long flexible branches.The most challenging feature for the mechanized harvest of oil-tea fruits is that its flower and fruit grow synchronously.In order to improve the harvesting efficiency and avoid damaging the flower bud,a hand-held fruit harvesting machine with a variable spacing comb brush was proposed.The harvesting machine can generate three kinds of actuation to detach fruit when it runs.The main actuation results from the brushing of multiple comb fingers.The other two kinds of actuation result from the beating of comb fingers on the fruits and the branches.The finger spacing of the comb brush can be adjusted consequently through moving the spacing adjusting crossbar.Hence,when the finger spacing is smaller than the diameter of the oil-tea fruit,the fruit is brushed off,but the flower bud and leaf pass through the finger gap.When the finger spacing is bigger than the fruit diameter,the fruit stuck between the fingers is loosened to ensure the continuous operation of the machine.Nylon was used as the material of the brush finger to avoid damage,which can also reduce the overall weight.The dynamic simulation of the harvesting machine was carried out with ADAMS,and the acceleration of the front end of the comb finger and the variation of the finger spacing were analyzed.The prototype of the harvesting machine was built and tested in the field.Field experiment results showed that when the speed of the comb finger drive shaft was 480 r/min,the average harvesting percentage of oil-tea fruit was 80%,and the flower bud was seldom detached,which met the working requirements of oil-tea fruit harvesting.
文摘Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat （ISSR）-PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showed that 493 discernible loci with distinct elec- trophoretic bands were obtained, of which, 478 loci （96.78%） were polymorphic. This indicated that oil-tea germplasms possess abundant genetic diversities. By clustering analysis performed using softwares of NTSYS 2.10 and Winboot, 51 oil-tea germplasms were divided into two groups： Group I had 48 lines of Camellia oleifera Abel, while Group II had three C. oleifera Abel related species and their similarity coefficient was 0.62. Group I was further divided into Group I-1 and Group I-2, and their similarity coefficient （Gs） was 0.634. All members of Group I-1 originated from Hunan Province, while Group I-2 included the rest of Hunan lines and those originated from other regions of China. Analyzed by software POPGENE 1.32, the Shannon＇s information index （I＊） of genetic polymorphism was 0.3852, the genetic diversity among different region popula- tions （Ht） was 0.2537, the genetic diversity within populations （Hs） was 0.15545, the dif- ferentiation coefficient of genetic diversity among populations （Gst） was 0.3967, and the gene flow among populations （Nm＊） was 0.8262. The Nei＇s genetic distances between the Hunan population and the populations originated from other regions of China implied that geographic isolation strongly influenced genetic differentiation among populations. Meanwhile, seedling rootstock grafting and high grafting for tree crown produced genetic variations among clonal offsprings.
