[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their ef...[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall（ chitin and phenoloxidase）,the physiological and biochemical indices acting on the nervous system（ Acetylcholinesterase）,and the physiological and biochemical indices acting on the digestive system（ protease,amylase and lipase） of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.展开更多
Abundant bitumen can be discovered in the wells of Upper Sinian Dengying Formation in the Sichuan Basin and their peripheral outcrops sections.As the cracking product of a paleo-oil reservoir,the occurrence morphologi...Abundant bitumen can be discovered in the wells of Upper Sinian Dengying Formation in the Sichuan Basin and their peripheral outcrops sections.As the cracking product of a paleo-oil reservoir,the occurrence morphologies and distribution characteristics of bitumen in carbonate reservoirs can indicate the preservation,migration and other key information of(paleo-)oil and gas reservoirs.In order to provide reference and basis for the deep and ultra-deep oil and gas exploration in the Sichuan Basin and the basins in western China,this paper systematically studies the occurrence morphological characteristics of bitumen in deep and ultra-deep carbonate reservoirs in well Gaoshi 1 of the Sichuan Basin by carrying out thin section observation,scanning electron microscope-energy spectrum analysis and fluid inclusion organic geochemical analysis on the cores of the fourth member of Dengying Formation(Deng 4 Member).In addition,the coupling relationship between the occurrence morphology of bitumen and the key event of hydrocarbon accumulation is revealed.And the following research results are obtained.First,solid bitumen in the Deng 4 Member deep and ultra-deep reservoir has two types of occurrence morphology.The first type of bitumen adheres to pore walls in the shape of a rim or exists in pores in the form of sticky branch(e.g.,Wells Chuanshen 1 and Gaoshi 1).There are more obvious shrinkage fractures and residual pores generated by in-situ thermal cracking in this type of bitumen.And combined with fluid inclusion and burial thermal history,it can effectively indicate that a paleo-gas reservoir has been preserved so far since the paleo-oil reservoir was thermally cracked into carbonaceous bitumen.The second type of bitumen is distributed in intra-and inter-neogenic mineral crystals in pores disorderly in the shape of grain and band(e.g.,Wells Mashen 1 and Wutan 1)and it has obvious breaking edges.It reveals that after a paleo-oil reservoir was thermally cracked into carbonaceous bitumen,the paleo-gas reservoir suffered blowdown and gas escape and neogenic fluid minerals moved into reservoirs to fill in pores.In conclusion,the occurrence morphologies and distribution characteristics of bitumen in deep and ultra-deep carbonate reservoirs can effectively indicate the hydrocarbon accumulation and evolution process of paleo-oil and gas reservoirs.展开更多
基金Supported by Open Fund of Key Laboratory of Contaminated Environment Con-trol and Regional Ecology Safety(SYU-KF-L-05)Science and TechnologyProject of Liaoning Province(2012214001)
文摘[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall（ chitin and phenoloxidase）,the physiological and biochemical indices acting on the nervous system（ Acetylcholinesterase）,and the physiological and biochemical indices acting on the digestive system（ protease,amylase and lipase） of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.
基金Project supported by the National Scientific Fund Project“Fundamental Study on Marine Deep-Layer Oil and Gas Enrichment Mechanism and Key Engineering Technologies”(No.U19B6003)Key Scientific and Technological Planning Research Project of the Sichuan Basin“Process and Mechanism of Hydrocarbon Accumulation in the Sinian Dengying Formation,the Sichuan Basin”(No.2018JZ0078)Scientific and Technological Planning Application Fundamental Research Project(No.2019YJ0500).
文摘Abundant bitumen can be discovered in the wells of Upper Sinian Dengying Formation in the Sichuan Basin and their peripheral outcrops sections.As the cracking product of a paleo-oil reservoir,the occurrence morphologies and distribution characteristics of bitumen in carbonate reservoirs can indicate the preservation,migration and other key information of(paleo-)oil and gas reservoirs.In order to provide reference and basis for the deep and ultra-deep oil and gas exploration in the Sichuan Basin and the basins in western China,this paper systematically studies the occurrence morphological characteristics of bitumen in deep and ultra-deep carbonate reservoirs in well Gaoshi 1 of the Sichuan Basin by carrying out thin section observation,scanning electron microscope-energy spectrum analysis and fluid inclusion organic geochemical analysis on the cores of the fourth member of Dengying Formation(Deng 4 Member).In addition,the coupling relationship between the occurrence morphology of bitumen and the key event of hydrocarbon accumulation is revealed.And the following research results are obtained.First,solid bitumen in the Deng 4 Member deep and ultra-deep reservoir has two types of occurrence morphology.The first type of bitumen adheres to pore walls in the shape of a rim or exists in pores in the form of sticky branch(e.g.,Wells Chuanshen 1 and Gaoshi 1).There are more obvious shrinkage fractures and residual pores generated by in-situ thermal cracking in this type of bitumen.And combined with fluid inclusion and burial thermal history,it can effectively indicate that a paleo-gas reservoir has been preserved so far since the paleo-oil reservoir was thermally cracked into carbonaceous bitumen.The second type of bitumen is distributed in intra-and inter-neogenic mineral crystals in pores disorderly in the shape of grain and band(e.g.,Wells Mashen 1 and Wutan 1)and it has obvious breaking edges.It reveals that after a paleo-oil reservoir was thermally cracked into carbonaceous bitumen,the paleo-gas reservoir suffered blowdown and gas escape and neogenic fluid minerals moved into reservoirs to fill in pores.In conclusion,the occurrence morphologies and distribution characteristics of bitumen in deep and ultra-deep carbonate reservoirs can effectively indicate the hydrocarbon accumulation and evolution process of paleo-oil and gas reservoirs.
