[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Ki...[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.展开更多
Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contr...Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contrasting day times:in the morning with the highest levels of leaf Se and polyphenol(TP)and the lowest dry matter,and in the evening with the opposite characteristics.Compared to the control,the morning Se supply demonstrated a higher increase of root biomass(1.27–1.37 times),Se(1.82–2.85 times),TP content(1.42–1.44 times),and antioxidant activity(AOA)(1.47–1.48 times)than the evening treatment.The latter did not affect root biomass and TP levels,but increased Se(1.38–2.57 times)and AOA(1.47–1.48 times).Contrary,compared to control,the evening Se supply improved leaf parameters more significantly than the morning treatment:AOA(1.22–1.25 vs.1.12–1.17),TP(1.29–1.33 vs.1.10–1.25),and Se(7.03–8.58 vs.5.32–7.19).Similar photosynthetic pigment increase was recorded under organic and inorganic Se supply and between morning and evening treatments.Contrasting trends in root disaccharide accumulation under Se supply were recorded between morning and evening treatments,with a significant decrease of the mentioned parameter in the former case(1.27–1.15 times)and an increase in the latter(1.11–1.31 times).Contrary to other dandelion characteristics,only disaccharide root levels demonstrated higher changes under selenocystine supply,compared to selenate.The revealed phenomenon indicates the differences in root/leaf biochemical profile response to the time of Se supplementation and may become the basis of targeted production of functional food products with improved yield and nutritional value.展开更多
T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in t...T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in this study. The content of electrolyte leakage was increasing in Dendrobium Sw. as the temperature decreasing, while the content of peroxidase(POD) activity and the soluble sugar showed a rising trend before a downward trend. Besides, external morphology was another evaluation standard of cold tolerance. Thus, there was a significant difference between Dendrobium officinal and other Dendrobium species in the cold tolerance, and their cold tolerance capacity was as follows: s1 > s100 >s13 > 1301 > s300 > Dendrobium nobile > s126 > s145 > s95 > Dendrobium lindleyi > Dendrobium chrysotoxum > Dendrobium thyrsiflorum > Dendrobium lohohense.展开更多
基金Supported by Plan Project of Science and Technology Committee of Guangxi Province (0322024-3A) Scientific Research Foundation for Returned Scholars of Guangxi Province~~
文摘[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
文摘Circadian biorhythms are fundamental in plant adaptability and development.To reveal the effect of organic and inorganic forms of Se,foliar treatments of dandelion with 0.26 mM Se solutions were practiced in two contrasting day times:in the morning with the highest levels of leaf Se and polyphenol(TP)and the lowest dry matter,and in the evening with the opposite characteristics.Compared to the control,the morning Se supply demonstrated a higher increase of root biomass(1.27–1.37 times),Se(1.82–2.85 times),TP content(1.42–1.44 times),and antioxidant activity(AOA)(1.47–1.48 times)than the evening treatment.The latter did not affect root biomass and TP levels,but increased Se(1.38–2.57 times)and AOA(1.47–1.48 times).Contrary,compared to control,the evening Se supply improved leaf parameters more significantly than the morning treatment:AOA(1.22–1.25 vs.1.12–1.17),TP(1.29–1.33 vs.1.10–1.25),and Se(7.03–8.58 vs.5.32–7.19).Similar photosynthetic pigment increase was recorded under organic and inorganic Se supply and between morning and evening treatments.Contrasting trends in root disaccharide accumulation under Se supply were recorded between morning and evening treatments,with a significant decrease of the mentioned parameter in the former case(1.27–1.15 times)and an increase in the latter(1.11–1.31 times).Contrary to other dandelion characteristics,only disaccharide root levels demonstrated higher changes under selenocystine supply,compared to selenate.The revealed phenomenon indicates the differences in root/leaf biochemical profile response to the time of Se supplementation and may become the basis of targeted production of functional food products with improved yield and nutritional value.
基金supported by the Three Biological Technology Co.,LTD in Tai’an,Shandong Province.
文摘T o investigate cold tolerance of different cultivars and species in Dendrobium Sw. in North China, 8 strains of Dendrobium officinal and other 5 Dendrobium species were used to compare their cold-hardiness index in this study. The content of electrolyte leakage was increasing in Dendrobium Sw. as the temperature decreasing, while the content of peroxidase(POD) activity and the soluble sugar showed a rising trend before a downward trend. Besides, external morphology was another evaluation standard of cold tolerance. Thus, there was a significant difference between Dendrobium officinal and other Dendrobium species in the cold tolerance, and their cold tolerance capacity was as follows: s1 > s100 >s13 > 1301 > s300 > Dendrobium nobile > s126 > s145 > s95 > Dendrobium lindleyi > Dendrobium chrysotoxum > Dendrobium thyrsiflorum > Dendrobium lohohense.
