Houpolignols A-C(1-3),unprecedented oligomers of dearomatized obovatol with tetracyclo[9.3.1.0^(2,7).0^(9,14)]pentadecane(1 and 2)and 8,18-dioxapentacyclo[13.3.1.1^(5,9).0^(4,16).0^(13,20)]icosane(3)core structures,to...Houpolignols A-C(1-3),unprecedented oligomers of dearomatized obovatol with tetracyclo[9.3.1.0^(2,7).0^(9,14)]pentadecane(1 and 2)and 8,18-dioxapentacyclo[13.3.1.1^(5,9).0^(4,16).0^(13,20)]icosane(3)core structures,together with their biosynthetic congener houpolignol D(4),were isolated from the cortex of Magnolia officinalis var.biloba.Their structures were determined by spectroscopic analyses,Xray crystallography data,and quantum chemical calculations.Radical cascade cyclizations were proposed as crucial biosynthetic steps of 1-4.(±)-1 showed anti-nonalcoholic steatohepatitis(NASH)effect by promoting fatty acid beta-oxidation.展开更多
Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis...Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis which was used as health foods and drugs.This paper summarized the active ingredients,pharmacological activities and molecular mechanisms of C.officinalis in recent 5 years to provide reference for the development and utilization of C.officinalis.A total of 149 active constituents of C.officinalis were summarized,including iridoids(64),flavonoids(18),lignin(17),tannins(16),organic acids and phenolic acids(14),triterpenes(11)and others(9),which were further subdivided according to their pharmacological effects.It was found that the pharmacological effects of C.officinalis were very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,neuroprotective and other biological activities.Among these activities,the anti-inflammatory and hypoglycemic mechanisms showed multi-pathway and multi-target characteristics,which were elaborated systematically.However,there are still many compounds in C.officinalis that have not been studied for biological activity,which means that it still has potential pharmacological activity to be further studied.C.officinalis not only has high medicinal value,but also has a potential edible resource.However,limited by people's understanding of the nutritional value of C.officinalis,few product types and incomplete processing technology,people's acceptance of the edible value of C.oficinalis is not high,and it needs to be further developed and utilized.展开更多
[Objectives]To analyze the differences in medicinal component contents of Magnolia officinalis across different altitude gradients and explore their causes.[Methods]In this experiment,M.officinalis trees aged 15-20 ye...[Objectives]To analyze the differences in medicinal component contents of Magnolia officinalis across different altitude gradients and explore their causes.[Methods]In this experiment,M.officinalis trees aged 15-20 years growing at four altitudes(1301,1444,1573,and 1643 m)were selected as experimental materials.Leaf traits,soil physicochemical properties,and medicinal component contents were investigated,and the relationships among leaf traits,soil physicochemical properties,and medicinal components were analyzed.[Results]With increasing altitude,the specific leaf area(SLA)of M.officinalis significantly increased,while stomatal density,vein density,leaf thickness,and mesophyll tissue thickness decreased.Soil total nitrogen(TN),total phosphorus(TP),total potassium(TK),available nitrogen(AN),and organic matter contents(OM)decreased significantly with altitude,whereas available potassium(AK)showed the opposite trend.The contents of medicinal components magnolol and honokiol in M.officinalis also significantly decreased with altitude.Correlation analysis revealed that,in addition to altitude,soil physicochemical properties(pH,TP,OM)and leaf traits(leaf thickness,palisade tissue thickness,SLA)were significantly correlated with magnolol and honokiol contents.[Conclusions]M.officinalis at lower altitudes exhibited better growth and higher magnolol and honokiol contents,which may be attributed to higher soil nutrient availability in low-altitude regions.This study provides guidance for selecting cultivation sites and optimizing planting patterns for M.officinalis.展开更多
A strategy combining a tailored database and high-throughput activity screening that discover bioactive metabolites derived from Magnoliae Officinalis Cortex(MOC)was developed and implemented to rapidly profile and di...A strategy combining a tailored database and high-throughput activity screening that discover bioactive metabolites derived from Magnoliae Officinalis Cortex(MOC)was developed and implemented to rapidly profile and discover bioactive metabolites in vivo derived from traditional Chinese medicine(TCM).The strategy possessed four characteristics:1)The tailored database consisted of metabolites derived from big data-originated reference compound,metabolites predicted in silico,and MOC chemical profile-based pseudomolecular ions.2)When profiling MOC-derived metabolites in vivo,attentions were paid not only to prototypes of MOC compounds and metabolites directly derived from MOC compounds,as reported by most papers,but also to isomerized metabolites and the degradation products of MOC compounds as well as their derived metabolites.3)Metabolite traceability was performed,especially to distinguish isomeric prototypes-derived metabolites,prototypes of MOC compounds as well as phase I metabolites derived from other MOC compounds.4)Molecular docking was utilized for high-throughput activity screening and molecular dynamic simulation as well as zebrafish model were used for verification.Using this strategy,134 metabolites were swiftly characterized after the oral administration of MOC to rats,and several metabolites were reported for the first time.Furthermore,17 potential active metabolites were discovered by targeting the motilin,dopamine D2,and the serotonin type 4(5-HT4)receptors,and part bioactivities were verified using molecular dynamic simulation and a zebrafish constipation model.This study extends the application of mass spectrometry(MS)to rapidly profile TCM-derived metabolites in vivo,which will help pharmacologists rapidly discover potent metabolites from a complex matrix.展开更多
Magnolia officinalis is a perennial deciduous tree that has medicinal properties.The AP2/ERF gene family has a number of roles in long-term growth and metabolism.The expression of this function varies with the growth ...Magnolia officinalis is a perennial deciduous tree that has medicinal properties.The AP2/ERF gene family has a number of roles in long-term growth and metabolism.The expression of this function varies with the growth period.In this work,based on the transcriptome data of Magnolia officinalis,the complete coding gene of Magnolia officinalis was obtained,and the corresponding protein sequence was retrieved from NCBI and compared with the model plant Arabidopsis thaliana.After screening,75 protein sequences from the AP2/ERF gene family were identified and called MoAP2/ERF1–MoAP2/ERF75,followed by bioinformatics analysis.75 AP2/ERF gene families were found and classified into four subfamilies.Their protein architectures had one or more conserved AP2 domains,which were typically unstable and hydrophilic.Subcellular research revealed that it was primarily located in the nucleus.Among them,the DREB subfamily showed stronger activity in the early growth period of Magnolia officinalis,suggesting that Magnolia officinalis had stronger resistance to adversity during this period.The 15 members of the MoAP2/ERF gene family showed significant differences during different growth periods,and they regulated the gene expression of Magnolia officinalis by binding to DNA.The 15 MoAP2/ERF gene families have a wide range of physiological activities in biological processes,cellular components,and molecular functions.Including MoAP2/ERF55 can catalyze imidazole glycerol phosphate synthase activity;MoAP2/ERF39 acts as a transcriptional activator of Pti6.展开更多
[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used t...[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used to normalize and standardize the targets.Liver cancer targets were searched through GeneCards,DISGENET,and other databases.Venny website was used to obtain the intersection target of valerian active ingredients and liver cancer disease.The protein-protein interaction(PPI)network of the intersection targets was analyzed by STRING database,and the PPI network was constructed by Cytoscape software.The David database was used for GO functional annotation and KEGG pathway enrichment analysis to obtain the relevant pathways in the treatment of liver cancer with Mongolian medicine V.officinalis.The corresponding chemical components,targets and pathways of liver cancer were imported into Cytoscape software to construct the network topology of"chemical component-disease-target-pathway".According to the analysis results,the potential of the active components in V.officinalis as a therapeutic drug for liver cancer was evaluated,and the correlation between the results of network pharmacology analysis and clinical treatment of liver cancer was discussed,which provided a reference for clinical application.[Results]A total of 13 kinds of chemical components and 108 drug disease intersection target genes were screened,and the core genes acting on diseases were caffeic acid,perillyl acetate,(+)-alpha-Terpineol,eucalyptol,etc.;GO functional enrichment analysis involved 389 items of biological processes,62 items of cellular components and 120 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out chemical carcinogenesis-receptor activation,cancer pathways,prolactin signaling pathways,proteoglycans in cancer,EGFR tyrosine kinase inhibitor resistance and other signaling pathways.[Conclusions]The mechanism of Mongolian medicine V.officinalis on liver cancer was studied by network pharmacology.It was found that it can inhibit the proliferation of liver cancer cells from multiple targets and pathways.This is expected to provide a theoretical basis for further basic experimental research.展开更多
Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability...Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.展开更多
Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officina...Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officinalis were analyzed by the method of HPLC (High Performance Liquid Chromatogram). The results showed that such qualitative traits as the content of magnolol, content of honokiol, total content of key phenols, and the ratio of magnolo to honokiol differ significantly between the provenances. The provenances with a sharpened leaf tip from the western part of Hubei Province has a highest content of phenols, and that with a concave leaf tip from the Lushan Mountain has a lowest content of phenols. All these four qualitative traits were genetically controlled, with a heritability between 0.8342 and 0.9871 in terms of provenance. In addition, both longitudinal and latitudinal geographical variations could be found, with longitudinal variations being dominant. As a result, 3 superior provenances from Wufeng, Enshi and Hefeng of the western part of Hubei as well as 10 high-quality individuals were selected.展开更多
Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Cori...Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.展开更多
AIM: The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves against extracellular(promastigote) and intracellular(amastigote) form...AIM: The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves against extracellular(promastigote) and intracellular(amastigote) forms of Leishmania major were evaluated in this study. METHOD: In the first stage, promastigote forms of L. major, were treated with different doses of the plant extracts in a 96-well tissue-culture microplate and IC50 values for each extract were measured with colorimetric MTT assay. In the second stage, macrophage cells were infected with L. major promastigotes. Infected macrophages were treated with plant extracts. Then the macrophages were stained with Gimsa and the number of infected macrophages and amastigotes were counted with a light microscope. RESULTS: The results indicated that the plant extracts inhibited the growth of promastigotes and amastigotes of L. major. Inhibitory concentrations(IC50) for promastigote assay were 108.19, 155.15, and 184.32 μg·mL-1 for C. officinalis flowers, D. stramonium seeds and S. officinalis, respectively. The extracts also reduced the number of amastigotes in macrophage cells from 264 for control group to 88, 97, and 102 for test groups. Although the anti-leishmanial activity of the extracts were not comparable with the standard drug, miltefosine; but they showed significant efficiency in reducing the number of amastigotes in macrophages, in comparison with the control group(P < 0.001). These plant extracts had lower toxicity compared with miltefosine. CONCLUSION: This study demonstrates the potential efficacy of the methanolic extracts of C. officinalis flowers, D. stramonium seeds, and S. officinalis leaves to control of cutaneous leishmaniasis.展开更多
[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition lin...[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition line were studied.[Method] The phenotype analysis was used to study the separation proportion of progeny.Moreover,SSR and the methylation analysis were used to study the transmission behavior of nonhomologous chromosome.[Result] 78 plants of 145 backcross progenies preserved the rolled leaf mark trait of MAAL8.In 32 anther culture plants,five plants had the normal rolled leaves,and two plants had the extremely rolled leaves.The rest had the flat leaves.14 couples of SSR markers were used to analyze,and it indicated that all the rolled-leaf plants could obtain the characteristic band type of O.officinalis,but the flat-leaf plants showed none of them.11 polymorphic RFLP markers were used to carry out Methylation-Sensitive Southern analysis.It showed that the methylation variation manners of eight markers between AA and CC genomes were different.[Conclusion] The nonhomologous chromosome of MAAL8 could pass to the progenies independently and integrally via the meiosis,and the phenotype characteristics didn＇t change.Moreover,the methylation manner of O.officinalis could inherit stably in the hybrid progeny as the addition of single chromosome.The stability of methylation might have the certain effect on the relatively independent inheritance of nonhomologous chromosome in the genome environment of O.sativa.展开更多
[Objective] The aim was to investigate the genetic diversity and analyze the genetic differentiation of germplasm resources of Gentiana officinalis H.Smith from Qinghai.[Method] The cpDNA of chloroplast psbA-trnH gene...[Objective] The aim was to investigate the genetic diversity and analyze the genetic differentiation of germplasm resources of Gentiana officinalis H.Smith from Qinghai.[Method] The cpDNA of chloroplast psbA-trnH gene was sequenced to analyze the genetic diversity of six populations of G.officinalis.[Result] A total of 10 distinct haplotypes were detected in the studied populations,and seven variable sites were found by comparing their sequences.G.officinalis with high-level genetic diversity（h=0.771）.Genetic diversity was largely varied within populations,ranging from 0.563 to 0.857 for haplotype diversity,and from 0.002 43 to 0.005 83 for nucleotide diversity,respectively.Genetic differentiation among populations（Gst） of G.officinalis was 0.196 0;gene flow（Nm） was 2.05;80.40% of the genetic variability occurred within population.[Conclusion] The cultivated G.officinalis in Qinghai showed rich genetic diversity,which is beneficial for the production of high-quality herb medicine.展开更多
To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field plant...To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.展开更多
A new phenylethanoid glycoside, neobudofficide B was isolated from Buddleia officinalis. On the basis of spectral and chemical evidence, the glycoside was identified as β ( 3′, 4′ dihydroxy, 6′ N pyridinechlo...A new phenylethanoid glycoside, neobudofficide B was isolated from Buddleia officinalis. On the basis of spectral and chemical evidence, the glycoside was identified as β ( 3′, 4′ dihydroxy, 6′ N pyridinechloride phenyl ) ethyl O α L rhamnopyranosyl ( 1→3 ) β D ( 4″ O caffeoyl ) glucopyranoside.展开更多
A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed ...A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed that both 38J9 and RZ69 were located in the middle of 4S in O. officinalis and the centromere area of 4S in O. sativa. In O. officinalis the percentage distances from the centromere to the hybridization sites were 20.00 +/- 5.40 and 22.12 +/- 3.44, the detection rates were 50.00% and 6.14%, but in O. sativa they were 0 and 0, 56.10% and 6.29% correspondingly. The results obtained from the BAC and RFLP clone were almost the same in the cultivated rice and O. officinalis. It was suggested that die marker RZ69 of the cultivated rice and its homologous sequence in O. officinalis were in the same BAC clone, the homologous sequence of Bph3 in O. officinalis should be located at the sites hybridized by both RZ69 and 38J9. Many signals on different chromosomes of O. officinalis were observed under no blocking with Cot-1 DNA, showing that the repetitive sequences were also homologous between O. sativa and O. officinalis. The identification of chromosome 4 of O. officinalis is based on comparative map with RG214 and BAC clone screened by RG214. The feasibility of comparative physical mapping performed between O. sativa and O. officinalis with rice BAC clones was discussed.展开更多
From the aerial parts ofNauclea officinalis （Pierre ex Pitard） Merr. et Churl, one new indole alkaloid paratunamide E （1） has been isolated together with six known indole alkaloids, paratunamide A （2）, nauclealo...From the aerial parts ofNauclea officinalis （Pierre ex Pitard） Merr. et Churl, one new indole alkaloid paratunamide E （1） has been isolated together with six known indole alkaloids, paratunamide A （2）, nauclealotide C （3）, nauclealotide A （4）, vincosamide （5）, strictosamide （6） and naucleamide F （7）. Naucleamide F （7） was isolated from Nauclea officinalis for the first time. All of the seven compounds above were elucidated by spectroscopic methods including 1 D and 2 D NMR soectroscoDic analyses.展开更多
基金Financial support for this study is from the National Natural Science Foundation of China(No.32170406)the Social Development Special Projects-Key Research and Development Plan of Science and Technology Department of Yunnan Province(No.202303AC100025)the 111 Project from Ministry of Education of China and the State Administration of Foreign Export Affairs of China(No.B18056).
文摘Houpolignols A-C(1-3),unprecedented oligomers of dearomatized obovatol with tetracyclo[9.3.1.0^(2,7).0^(9,14)]pentadecane(1 and 2)and 8,18-dioxapentacyclo[13.3.1.1^(5,9).0^(4,16).0^(13,20)]icosane(3)core structures,together with their biosynthetic congener houpolignol D(4),were isolated from the cortex of Magnolia officinalis var.biloba.Their structures were determined by spectroscopic analyses,Xray crystallography data,and quantum chemical calculations.Radical cascade cyclizations were proposed as crucial biosynthetic steps of 1-4.(±)-1 showed anti-nonalcoholic steatohepatitis(NASH)effect by promoting fatty acid beta-oxidation.
基金supported by National Key R&D Program of China(2022YFF1100300)Major Science and Technology Project of Henan Province(231100310200)+1 种基金Key R&D Project of Henan Province(231111112100)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Cornus officinalis Sieb.et Zucc.,a valuable Chinese medicine resource,has a long clinic utilization history.In recent years,more research focus on the chemical composition and pharmacological activity of C.officinalis which was used as health foods and drugs.This paper summarized the active ingredients,pharmacological activities and molecular mechanisms of C.officinalis in recent 5 years to provide reference for the development and utilization of C.officinalis.A total of 149 active constituents of C.officinalis were summarized,including iridoids(64),flavonoids(18),lignin(17),tannins(16),organic acids and phenolic acids(14),triterpenes(11)and others(9),which were further subdivided according to their pharmacological effects.It was found that the pharmacological effects of C.officinalis were very extensive,mainly including antioxidant,anti-inflammatory,anti-tumor,neuroprotective and other biological activities.Among these activities,the anti-inflammatory and hypoglycemic mechanisms showed multi-pathway and multi-target characteristics,which were elaborated systematically.However,there are still many compounds in C.officinalis that have not been studied for biological activity,which means that it still has potential pharmacological activity to be further studied.C.officinalis not only has high medicinal value,but also has a potential edible resource.However,limited by people's understanding of the nutritional value of C.officinalis,few product types and incomplete processing technology,people's acceptance of the edible value of C.oficinalis is not high,and it needs to be further developed and utilized.
基金Supported by National Key Research and Development Program of China in 2018(2018YFC1708005)the Southwest Minzu University Research Startup Funds(RQD2023020).
文摘[Objectives]To analyze the differences in medicinal component contents of Magnolia officinalis across different altitude gradients and explore their causes.[Methods]In this experiment,M.officinalis trees aged 15-20 years growing at four altitudes(1301,1444,1573,and 1643 m)were selected as experimental materials.Leaf traits,soil physicochemical properties,and medicinal component contents were investigated,and the relationships among leaf traits,soil physicochemical properties,and medicinal components were analyzed.[Results]With increasing altitude,the specific leaf area(SLA)of M.officinalis significantly increased,while stomatal density,vein density,leaf thickness,and mesophyll tissue thickness decreased.Soil total nitrogen(TN),total phosphorus(TP),total potassium(TK),available nitrogen(AN),and organic matter contents(OM)decreased significantly with altitude,whereas available potassium(AK)showed the opposite trend.The contents of medicinal components magnolol and honokiol in M.officinalis also significantly decreased with altitude.Correlation analysis revealed that,in addition to altitude,soil physicochemical properties(pH,TP,OM)and leaf traits(leaf thickness,palisade tissue thickness,SLA)were significantly correlated with magnolol and honokiol contents.[Conclusions]M.officinalis at lower altitudes exhibited better growth and higher magnolol and honokiol contents,which may be attributed to higher soil nutrient availability in low-altitude regions.This study provides guidance for selecting cultivation sites and optimizing planting patterns for M.officinalis.
基金supported by the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences,China(Grant Nos.:CI2023E002 and CI2021A04513)the National Natural Science Foundation of China(Grant Nos.:82204619 and 82274094)the Fundamental Research Funds for the Central Public Welfare Research Institutes,China(Grant Nos.:ZZ15-YQ-067 and ZZ16-ND-10-26).
文摘A strategy combining a tailored database and high-throughput activity screening that discover bioactive metabolites derived from Magnoliae Officinalis Cortex(MOC)was developed and implemented to rapidly profile and discover bioactive metabolites in vivo derived from traditional Chinese medicine(TCM).The strategy possessed four characteristics:1)The tailored database consisted of metabolites derived from big data-originated reference compound,metabolites predicted in silico,and MOC chemical profile-based pseudomolecular ions.2)When profiling MOC-derived metabolites in vivo,attentions were paid not only to prototypes of MOC compounds and metabolites directly derived from MOC compounds,as reported by most papers,but also to isomerized metabolites and the degradation products of MOC compounds as well as their derived metabolites.3)Metabolite traceability was performed,especially to distinguish isomeric prototypes-derived metabolites,prototypes of MOC compounds as well as phase I metabolites derived from other MOC compounds.4)Molecular docking was utilized for high-throughput activity screening and molecular dynamic simulation as well as zebrafish model were used for verification.Using this strategy,134 metabolites were swiftly characterized after the oral administration of MOC to rats,and several metabolites were reported for the first time.Furthermore,17 potential active metabolites were discovered by targeting the motilin,dopamine D2,and the serotonin type 4(5-HT4)receptors,and part bioactivities were verified using molecular dynamic simulation and a zebrafish constipation model.This study extends the application of mass spectrometry(MS)to rapidly profile TCM-derived metabolites in vivo,which will help pharmacologists rapidly discover potent metabolites from a complex matrix.
基金supported by the National Natural Science Foundation of China(num-ber 81202933)the Natural Science Foundation of Sichuan Province(number 2025ZNSFSC0205 and number 2022NSFSC0592)the Science and Technology Plan Project of Mianyang City(number 2018YFZJ025).
文摘Magnolia officinalis is a perennial deciduous tree that has medicinal properties.The AP2/ERF gene family has a number of roles in long-term growth and metabolism.The expression of this function varies with the growth period.In this work,based on the transcriptome data of Magnolia officinalis,the complete coding gene of Magnolia officinalis was obtained,and the corresponding protein sequence was retrieved from NCBI and compared with the model plant Arabidopsis thaliana.After screening,75 protein sequences from the AP2/ERF gene family were identified and called MoAP2/ERF1–MoAP2/ERF75,followed by bioinformatics analysis.75 AP2/ERF gene families were found and classified into four subfamilies.Their protein architectures had one or more conserved AP2 domains,which were typically unstable and hydrophilic.Subcellular research revealed that it was primarily located in the nucleus.Among them,the DREB subfamily showed stronger activity in the early growth period of Magnolia officinalis,suggesting that Magnolia officinalis had stronger resistance to adversity during this period.The 15 members of the MoAP2/ERF gene family showed significant differences during different growth periods,and they regulated the gene expression of Magnolia officinalis by binding to DNA.The 15 MoAP2/ERF gene families have a wide range of physiological activities in biological processes,cellular components,and molecular functions.Including MoAP2/ERF55 can catalyze imidazole glycerol phosphate synthase activity;MoAP2/ERF39 acts as a transcriptional activator of Pti6.
基金Supported by National Natural Science Foundation of China(82260844)Natural Science Foundation of Inner Mongolia(2022LHMS08021).
文摘[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used to normalize and standardize the targets.Liver cancer targets were searched through GeneCards,DISGENET,and other databases.Venny website was used to obtain the intersection target of valerian active ingredients and liver cancer disease.The protein-protein interaction(PPI)network of the intersection targets was analyzed by STRING database,and the PPI network was constructed by Cytoscape software.The David database was used for GO functional annotation and KEGG pathway enrichment analysis to obtain the relevant pathways in the treatment of liver cancer with Mongolian medicine V.officinalis.The corresponding chemical components,targets and pathways of liver cancer were imported into Cytoscape software to construct the network topology of"chemical component-disease-target-pathway".According to the analysis results,the potential of the active components in V.officinalis as a therapeutic drug for liver cancer was evaluated,and the correlation between the results of network pharmacology analysis and clinical treatment of liver cancer was discussed,which provided a reference for clinical application.[Results]A total of 13 kinds of chemical components and 108 drug disease intersection target genes were screened,and the core genes acting on diseases were caffeic acid,perillyl acetate,(+)-alpha-Terpineol,eucalyptol,etc.;GO functional enrichment analysis involved 389 items of biological processes,62 items of cellular components and 120 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out chemical carcinogenesis-receptor activation,cancer pathways,prolactin signaling pathways,proteoglycans in cancer,EGFR tyrosine kinase inhibitor resistance and other signaling pathways.[Conclusions]The mechanism of Mongolian medicine V.officinalis on liver cancer was studied by network pharmacology.It was found that it can inhibit the proliferation of liver cancer cells from multiple targets and pathways.This is expected to provide a theoretical basis for further basic experimental research.
文摘Based on the meteorological data of 24 automatic stations in Xinfeng County,combined with the climatic conditions for the growth of Morinda officinalis F.C.How and Sarcandra glabra(Thunb.)Nakai,the climate suitability zoning indicators of M.officinalis and S.glabra in Xinfeng County were constructed by relevant statistical methods,and the climate suitability zoning indicators were divided into four grades:most suitable,suitable,sub-suitable and unsuitable.According to the results of expert scores,the relevant growth impact factors were assigned weight values.Based on geographic information system(GIS),the climate suitability zoning of M.officinalis and S.glabra planting in Xinfeng County was carried out.The results show that most areas of Xinfeng County were suitable for planting M.officinalis and S.glabra.Among them,the most suitable planting areas of M.officinalis were mainly distributed in most of Huilong Town,from Jiangnan Community of Fengcheng Street to Yuntianhai area of Meikeng Town,Matouwantian Village and areas to the south,and areas to the south of Zhutong Village,Fengcheng Street.The most suitable areas for S.glabra planting were mainly distributed in Puchang Village of Huilong Town,the northeast of Huangzhai Town,the south of Fengcheng Street,and the central-southern part of Matou Town.The zoning results provide a scientific basis for the rational layout of M.officinalis and S.glabra planting in Xinfeng County.
文摘Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officinalis were analyzed by the method of HPLC (High Performance Liquid Chromatogram). The results showed that such qualitative traits as the content of magnolol, content of honokiol, total content of key phenols, and the ratio of magnolo to honokiol differ significantly between the provenances. The provenances with a sharpened leaf tip from the western part of Hubei Province has a highest content of phenols, and that with a concave leaf tip from the Lushan Mountain has a lowest content of phenols. All these four qualitative traits were genetically controlled, with a heritability between 0.8342 and 0.9871 in terms of provenance. In addition, both longitudinal and latitudinal geographical variations could be found, with longitudinal variations being dominant. As a result, 3 superior provenances from Wufeng, Enshi and Hefeng of the western part of Hubei as well as 10 high-quality individuals were selected.
文摘Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.
文摘AIM: The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers, Datura stramonium seeds, and Salvia officinalis leaves against extracellular(promastigote) and intracellular(amastigote) forms of Leishmania major were evaluated in this study. METHOD: In the first stage, promastigote forms of L. major, were treated with different doses of the plant extracts in a 96-well tissue-culture microplate and IC50 values for each extract were measured with colorimetric MTT assay. In the second stage, macrophage cells were infected with L. major promastigotes. Infected macrophages were treated with plant extracts. Then the macrophages were stained with Gimsa and the number of infected macrophages and amastigotes were counted with a light microscope. RESULTS: The results indicated that the plant extracts inhibited the growth of promastigotes and amastigotes of L. major. Inhibitory concentrations(IC50) for promastigote assay were 108.19, 155.15, and 184.32 μg·mL-1 for C. officinalis flowers, D. stramonium seeds and S. officinalis, respectively. The extracts also reduced the number of amastigotes in macrophage cells from 264 for control group to 88, 97, and 102 for test groups. Although the anti-leishmanial activity of the extracts were not comparable with the standard drug, miltefosine; but they showed significant efficiency in reducing the number of amastigotes in macrophages, in comparison with the control group(P < 0.001). These plant extracts had lower toxicity compared with miltefosine. CONCLUSION: This study demonstrates the potential efficacy of the methanolic extracts of C. officinalis flowers, D. stramonium seeds, and S. officinalis leaves to control of cutaneous leishmaniasis.
基金Supported by Hubei Natural Science Fund Item(2008CDB392)Natural Science Fund of South-Central University for Nationalities(yzz08005)~~
文摘[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition line were studied.[Method] The phenotype analysis was used to study the separation proportion of progeny.Moreover,SSR and the methylation analysis were used to study the transmission behavior of nonhomologous chromosome.[Result] 78 plants of 145 backcross progenies preserved the rolled leaf mark trait of MAAL8.In 32 anther culture plants,five plants had the normal rolled leaves,and two plants had the extremely rolled leaves.The rest had the flat leaves.14 couples of SSR markers were used to analyze,and it indicated that all the rolled-leaf plants could obtain the characteristic band type of O.officinalis,but the flat-leaf plants showed none of them.11 polymorphic RFLP markers were used to carry out Methylation-Sensitive Southern analysis.It showed that the methylation variation manners of eight markers between AA and CC genomes were different.[Conclusion] The nonhomologous chromosome of MAAL8 could pass to the progenies independently and integrally via the meiosis,and the phenotype characteristics didn＇t change.Moreover,the methylation manner of O.officinalis could inherit stably in the hybrid progeny as the addition of single chromosome.The stability of methylation might have the certain effect on the relatively independent inheritance of nonhomologous chromosome in the genome environment of O.sativa.
基金Supported by Science Foundation for the Excellent Youth and Middle-aged Scholars in Qinghai University(2009-QY-19)~~
文摘[Objective] The aim was to investigate the genetic diversity and analyze the genetic differentiation of germplasm resources of Gentiana officinalis H.Smith from Qinghai.[Method] The cpDNA of chloroplast psbA-trnH gene was sequenced to analyze the genetic diversity of six populations of G.officinalis.[Result] A total of 10 distinct haplotypes were detected in the studied populations,and seven variable sites were found by comparing their sequences.G.officinalis with high-level genetic diversity（h=0.771）.Genetic diversity was largely varied within populations,ranging from 0.563 to 0.857 for haplotype diversity,and from 0.002 43 to 0.005 83 for nucleotide diversity,respectively.Genetic differentiation among populations（Gst） of G.officinalis was 0.196 0;gene flow（Nm） was 2.05;80.40% of the genetic variability occurred within population.[Conclusion] The cultivated G.officinalis in Qinghai showed rich genetic diversity,which is beneficial for the production of high-quality herb medicine.
文摘To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is ＇Sligen 1 ＂, an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry （GC-MS） and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.
文摘A new phenylethanoid glycoside, neobudofficide B was isolated from Buddleia officinalis. On the basis of spectral and chemical evidence, the glycoside was identified as β ( 3′, 4′ dihydroxy, 6′ N pyridinechloride phenyl ) ethyl O α L rhamnopyranosyl ( 1→3 ) β D ( 4″ O caffeoyl ) glucopyranoside.
文摘A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed that both 38J9 and RZ69 were located in the middle of 4S in O. officinalis and the centromere area of 4S in O. sativa. In O. officinalis the percentage distances from the centromere to the hybridization sites were 20.00 +/- 5.40 and 22.12 +/- 3.44, the detection rates were 50.00% and 6.14%, but in O. sativa they were 0 and 0, 56.10% and 6.29% correspondingly. The results obtained from the BAC and RFLP clone were almost the same in the cultivated rice and O. officinalis. It was suggested that die marker RZ69 of the cultivated rice and its homologous sequence in O. officinalis were in the same BAC clone, the homologous sequence of Bph3 in O. officinalis should be located at the sites hybridized by both RZ69 and 38J9. Many signals on different chromosomes of O. officinalis were observed under no blocking with Cot-1 DNA, showing that the repetitive sequences were also homologous between O. sativa and O. officinalis. The identification of chromosome 4 of O. officinalis is based on comparative map with RG214 and BAC clone screened by RG214. The feasibility of comparative physical mapping performed between O. sativa and O. officinalis with rice BAC clones was discussed.
基金National Natural Science Foundation of China(Grant No.30973628)
文摘From the aerial parts ofNauclea officinalis （Pierre ex Pitard） Merr. et Churl, one new indole alkaloid paratunamide E （1） has been isolated together with six known indole alkaloids, paratunamide A （2）, nauclealotide C （3）, nauclealotide A （4）, vincosamide （5）, strictosamide （6） and naucleamide F （7）. Naucleamide F （7） was isolated from Nauclea officinalis for the first time. All of the seven compounds above were elucidated by spectroscopic methods including 1 D and 2 D NMR soectroscoDic analyses.
