The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The...The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The results showed that DOP improved glycolipid metabolism and serum inflammation levels,and inhibited intestinal-derived lipopolysaccharide(LPS)translocation,suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM.Interestingly,the study found that DOP reduced intestinal inflammation and oxidative stress levels,significantly up-regulated the mRNA expression of tight junction proteins Claudin-1,Occludin and zonula occluden-1(ZO-1),and ameliorated intestinal epithelial damage.In addition,DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter,Enterococcus and Desulfovibrio with a reduction rate of 95%,73% and 9%,respectively,and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%,respectively.Taken together,the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier,and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiotamodulatory effects,providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.展开更多
Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including ...Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including thick leaves and stems containing abundant polysaccharides and colloidal substances.Despite these adaptations,the underlying molecular mechanisms responsible for enhanced drought tolerance remain inadequately understood.This study subjected D.officinale to water restriction for periods ranging from 1 to 6 months,conducting physiological and RNA sequencing analyses to elucidate its long-term dehydration response mechanisms and identify drought-protective genes.Following 6 months of dehydration,D.officinale maintained viability,demonstrated by rapid growth resumption after merely 2 d of rehydration.Transcriptome analysis of D.officinale plants under 1-month dehydration revealed differential gene expression across various processes,predominantly in stress responses,photosynthesis,phytohormone signaling,carbon metabolism,and fructose/mannose pathways.Among these,PEROXIDASE4(POD4)and NAC37 showed significant upregulation and were selected for further investigation of their roles in drought protection.Transgenic tomato plants overexpressing D.officinale’s POD4 and NAC37 genes exhibited superior drought tolerance compared to controls,displaying enhanced vigor,increased fruit production,higher respiration rates,elevated chlorophyll levels,and reduced oxidative damage.This research demonstrates the value of exploring underutilized species for drought-tolerance genes and identifies POD4 and NAC37 as promising candidates for improving drought tolerance through breeding programs.展开更多
Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis...Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.展开更多
Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to exp...Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to explore the therapeutic effect of Dendrobium officinale(D.officinale)on skin aging.Methods:The ingredients of D.officinale were detected by UHPLC-Q-TOF/MS.The targets of D.officinale were screened by Swiss Target Prediction database.GeneCards,NCBI,and OMIM databases were utilized to find out the targets associated with skin photoaging.Overlapping targets of D.officinale and skin photoaging were obtained by Venn analysis.The ingredient-disease target network and protein-protein interaction network were constructed by using the STRING database and Cytoscape software.The key compounds and hub genes were obtained by analyzing networks.The DAVID database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the overlapping targets.Autodock Vina software was used to simulate molecular docking and the results were visualized using Pymol.Finally,the skin photoaging models of cells and mice were established to validate the results predicted by network pharmacology.Results:In D.officinale,a total of 59 compounds and 595 targets were detected,of which 59 proteins were intersectional with skin photoaging targets.The top 10 active ingredients(Dendrophenol,Herbacetin,Lyoniresinol,Trans-ferulaldehyde,Naringenin,and so on)and 8 hub genes(AKT1,TNF,VEGFA,MAPK3,CASP3,MMP9,CTNNB1,and EGFR)were identified.All the key active compounds could bind well with core protein targets(binding energy<-5 kcal/mol).The potential therapeutic targets were related to the response to reactive oxygen species,collagen catabolic process,extracellular matrix organization,and apoptotic process,mainly.We also found that D.officinale could enhance the cell viability and activity of anti-oxidases,reduce reactive oxygen species and MMP9 levels,and stable mitochondrial membrane potential.Furthermore,D.officinale could alleviate skin photoaging injury and reduce malondialdehyde level in mice.Conclusion:D.officinale alleviated skin photoaging via regulating oxidative stress,apoptosis,and collagen catabolic process.展开更多
The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results ...The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results of the study revealed several noteworthy findings.First,DOPS and DOPS100 treatments led to significant improvement in weight gain and reversal of fatigue-related behaviors compared to the normal group.Second,both DOPS and DOPS100 showed effectiveness in reducing immune organ swelling,modulating immunoglobulin A(IgA)and immunoglobulin M(IgM)levels,and restoring complement(C3c and C4)levels.In addition,they demonstrated a significant ability to enhance the integrity of the intestinal mechanical barrier by differentially upregulating the tight junction proteins Occludin and Zonula occludens 1(ZO-1).In addition,it was found that DOPS100 specifically enhanced the CD4^(+)-T helper 17 cell(Th17)/regulatory T cell(Treg)immune axis in the gut,as evidenced by increased expression of forkhead box protein 3(Foxp3)as well as decreased expression of retinoic acid receptor-related orphan receptor γt(RORγt),and further modulation of interleukin 10(IL-10),interleukin 22(IL-22),and interleukin 17A(IL-17A)expression levels of inflammatory factors.These findings collectively suggest that DOPS100 holds significant potential in improving sub-healthy status by repairing the intestinal barrier,restoring local immune homeostasis,and activating the intestinal immune regulatory network.The study’s outcomes provide valuable insights into the therapeutic implications of DOPS and DOPS100 in addressing immune dysfunction in sub-healthy conditions.展开更多
[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification f...[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification from mycorrhiza of wild Dendrobium officinale and inoculated on the root system of Dendrobium officinale for inoculation test. [Result] Under tissue-culture conditions, at early stage, Tj1 strain hadn＇t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tj1 strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets; under outdoor conditions, after inoculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [Conclusion] The two inoculation methods both indicate that Tj2 strain has relatively strong promotion effects on the growth of Dendrobium officinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain has practical application values on the successful cultivation of Dendrobium officinale plantlets.展开更多
The bioassay was directed to the fractionation of the methanol extract of the rhizomes of Zingiber officinale Roscoe leading to the isolation of a new antioxidant cyclic diarylheptanoid. The structure of the new compo...The bioassay was directed to the fractionation of the methanol extract of the rhizomes of Zingiber officinale Roscoe leading to the isolation of a new antioxidant cyclic diarylheptanoid. The structure of the new compound was established as 1, 5-epoxy-3-hydroxy-1-(3,4-dihydroxy-5-methoxy-phenyl)-7-(3,4-dihydroxyphenyl) heptane (1) on the basis of MS, 1D and 2D-NMR experiments.展开更多
In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 ...In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 kg and 113.32%, accordingly. The seedlings of Dendrobium officinale interplanted with Pleurotus os- treatus grew by 2.78 cm during 10 weeks on cultivation beds, and the seedlings grew by 2.68 cm in the cultivation beds without interplanting arrangement. These demonstrated that the interplanting of Dendrobium officinale and Pleurotus ostreatus has none adverse effects on growth of Dendrobium officinale.展开更多
为了深入研究豆瓣菜(Nasturtium officinale R. Br.)对锌和铁的吸收和转运,我们采用RACE方法克隆了一种豆瓣菜根部锌铁转运蛋白NoZIP2 (N. officinale zinc iron transporter protein 2)的全长cDNA,并采用生物信息学方法对序列进行分析...为了深入研究豆瓣菜(Nasturtium officinale R. Br.)对锌和铁的吸收和转运,我们采用RACE方法克隆了一种豆瓣菜根部锌铁转运蛋白NoZIP2 (N. officinale zinc iron transporter protein 2)的全长cDNA,并采用生物信息学方法对序列进行分析。结果表明,NoZIP2包含1 446 bp,编码一个357个氨基酸残基的多肽,包含了9个跨膜结构域,存在植物锌铁转运蛋白的保守结构域,可能参与了豆瓣菜锌铁转运的过程。NoZIP2氨基酸序列与其它物种ZIP的氨基酸序列相似性较高,其中与荠菜的相似性最高,氨基酸相似性高达92%。分子进化分析表明,NoZIP2与十字花科植物荠菜、荠蓝和小花蓝芥的ZIP能够聚到一起。从豆瓣菜根部克隆的NoZIP2具有其它植物锌铁转运蛋白相似的高度保守的ZIP家族成员序列特征,因此推测NoZIP2属于ZIP家族成员。豆瓣菜No ZIP2的克隆和序列分析能够为进一步研究其功能及表达提供理论基础。展开更多
[Objective] This study was conducted to provide scientific basis for fertilization of ginger with high quality and high yield. [Method] The soil nutrient contents and plant nutrient characteristics of Zhugen ginger（Z...[Objective] This study was conducted to provide scientific basis for fertilization of ginger with high quality and high yield. [Method] The soil nutrient contents and plant nutrient characteristics of Zhugen ginger（Zingiber officinale Rosc.） were studied. [Result] The results showed that： the soil of ginger planting area in Chongqing（Yongchuan） Seedlings of science and technology city was acidic overall,the soil organic matter content was low and alkali-hydrolyzable N was at moderate level; and the contents of available P, Ca, Mg, Fe, Mn, Cu and Zn were extremely rich, while the available S was in lack. The contents of N and K were abundant and P was little in ginger plants, the content of K was more than N in ginger roots and stems, while the content of N was more than K in ginger leaves. [Conclusion]In practical production, it was necessary to fertilize rationally and control the ratio of N fertilizer to K fertilizer according to local conditions, thereby promoting ginger growth and realize high-yield ginger production.展开更多
Objective:To evaluate the antibacterial properties ot Allium sativum(garlic) cloves and Zingiber officinale(ginger) rhizomes against multi-drug resistant clinical pathogens causing nosocomial infection.Methods:The clo...Objective:To evaluate the antibacterial properties ot Allium sativum(garlic) cloves and Zingiber officinale(ginger) rhizomes against multi-drug resistant clinical pathogens causing nosocomial infection.Methods:The cloves of garlic and rhizomes of ginger were extracted with 95%(v/v) ethanol.The ethanolic extracts were subjected to antibacterial sensitivity test against clinical pathogens.Results:Anti-bacterial potentials of the extracts of two crude garlic cloves and ginger rhizomes were tested against five gram negative and two gram positive multi-drug resistant bacteria isolates.All the bacterial isolates were susceptible to crude extracts of both plants extracts.Except Enterobacter sp.and Klebsiella sp.,all other isolates were susceptible when subjected to ethanolic extracts of garlic and ginger.The highest inhibition zone was observed with garlic(19.4S mm) against Pseudomonas aeruginosa(P.aeruginosa).The minimal inhibitory concentration was as low as 67.00 μg/mL against P.aeruginosa.Conclusions:Natural spices of garlic and ginger possess effective anti-bacterial activity against multi-drug clinical pathogens and can be used for prevention of drug resistant microbial diseases and further evaluation is necessary.展开更多
Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usu...Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usually a difficult task. In this study,we developed a rapid identification protocol for D. officinale using the loop-mediated isothermal amplification(LAMP) method. A set of primers were specifically designed to detect a modified internal transcribed spacer region of D. officinale at 65 ℃ within 40 min after adding SYBR~? Green I, which was used for the detection of D. officinale. Unlike commonly used adulterants, reaction mixtures containing D. officinale DNA changed from orange to green, and this color change was easily observed with the naked eye. Thus, this methodology can be used to accurately differentiate D. officinale from other Dendrobium species, is quick as all D. officinale samples were amplified within 40 min, and specific as samples of the adulterants were not amplified. The specificity of this LAMP-based method was confirmed by testing 17 samples of D. officinale and 32 adulterant samples from other Dendrobium species. This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.展开更多
OBJECTIVE： The present study is aimed at evaluating the protective effects of oils from Zingiber officinale （ginger） and Curcuma Ionga （turmeric） on acute ethanol-induced fatty liver in male Wistar rats. METHODS...OBJECTIVE： The present study is aimed at evaluating the protective effects of oils from Zingiber officinale （ginger） and Curcuma Ionga （turmeric） on acute ethanol-induced fatty liver in male Wistar rats. METHODS： Ferric reducing antioxidant power activity and oxygen radical absorbance capacity of the oils were evaluated ex vivo. Rats were pretreated for 28 d with standard drug （Livolin Forte） and oils from Z. officinale and C. Ionga before they were exposed to 45% ethanol （4.8 g/kg） to induce acute fatty liver. Histological changes were observed and the degree of protection was measured by using biochemical parameters such as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities. Serum triglyceride （TG） level, total cholesterol （TC） level and the effects of both oils on reduced gluthatione （GSH）, glutathione-S- transferase （GST）, superoxide dismutase （SOD） and hepatic malondialdehyde （MDA） levels were estimated. RESULTS： Oils from Z. officinale and C. Ionga at a dose of 200 mg/kg showed hepatoprotection by decreasing the activities of serum enzymes, serum TG, serum TC and hepatic MDA, while they significantly restored the level of GSH as well as GST and SOD activities. Histological examination of rats tissues was related to the obtained results. CONCLUSION： From the results it may be concluded that oils from Z. officinale and C. Ionga （200 mg/kg） exhibited hepatoprotective activity in acute ethanol-induced fatty liver and Z. officinale oil was identified to have better effects than C. Ionga oil.展开更多
Dendrobium officinale Kimura et Migo(D. officinale) is a famous traditional Chinese medicine(TCM). A mixture of D. officinale and American ginseng has been shown to enhance cell-mediated immunity, humoral immunity, an...Dendrobium officinale Kimura et Migo(D. officinale) is a famous traditional Chinese medicine(TCM). A mixture of D. officinale and American ginseng has been shown to enhance cell-mediated immunity, humoral immunity, and monocyte/macrophage functions in mice. Here, the effects of a D. officinale and American ginseng mixture on the structure of gut microbial community in dogs were examined using high-throughput 16S rRNA gene amplicon sequencing. The data revealed that while the mixture did not change the diversity of gut microbial community significantly, differences among individuals were significantly reduced. Furthermore, the mixture-responsive operational taxonomic units(OTUs) exhibited a phase-dependent expression pattern. Fifty-five OTUs were found to exhibit a mixture-induced expression pattern, among which one third were short-chain fatty acid(SCFA)-producing genera and the others were probiotic genera included Lactobacillus spp., Sutterella, Alistipes, Anaerovorax, Bilophila, Coprococcus, Gordonibacter, Oscillibacter, among others. By contrast, 36% of the OTUs exhibiting a mixture-repressed expression pattern were disease-associated microorganisms, and six genera, namely Actinomyces, Escherichia/Shigella, Fusobacterium, Slackia, Streptococcus and Solobacterium, were associated with cancer. In addition, five genera were closely associated with diabetes, namely Collinsella,Rothia, Howardella, Slackia and Intestinibacter. Our results indicate that this D. officinale and American ginseng mixture may be used as a prebiotic agent to enhance SCFA-producing genera and prevent gut dysbiosis.展开更多
Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient co...Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient content and high cost,limiting the use of D. officinale.Studying the network structure and rate-limiting steps of secondary metabolites of medicinal components of D. officinale,analyzing the secondary metabolic synthesis process,mastering the production rules of its medicinal components and carrying out gene cloning or biosynthesis,etc.are of great significance for the rational development and utilization of D. officinale resources. This paper briefly reviews the progress of the research on the secondary metabolites of D. officinale,including the detection and identification of metabolites and the identification and cloning of key metabolic enzymes.展开更多
Aim: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. Methods: The aqueous extracts of the two plants were gavaged separately...Aim: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. Methods: The aqueous extracts of the two plants were gavaged separately to 2 groups of rats at a similar dose of 600 mg.kg^1.day-1 for 8 days. At the end of the treatment, the animals were killed and the blood, testis, epididymis, seminal vesicles and prostate were collected for biochemical analysis. Results: The aqueous extract of Z. officinale significantly increased in the relative weight of the testis, the serum testosterone level, testicular cholesterol level and epididymal α-glucosidase activity. The aqueous extract of P. brazzeana significantly increased the weights of the testis, seminal vesicles and prostate. It also significantly increased the serum and testicular testosterone level. The fructose, α-glucosidase and cholesterol levels in P. brazzeanatreated rats were increased by 28%, 35% and 114%, respectively. Conclusion: The aqueous extracts of both P. brazzeana and Z. officinale have an androgenic activity, which seems to be more potent with P. brazzeana than with Z. officinale. (Asian J Androl 2002 Dec; 4: 299-301)展开更多
A new cyclic diarylheptanoid, 1,5-epoxy-3-hydroxy-1-（3-methoxy-4,5-dihydroxyphenyl）-7-（4-hydroxyphenyl）-heptane （1）, as well as a new monoterpene, 10-O-β-n-glucopyranosyl-hydroxy cineole （2） were isolated fro...A new cyclic diarylheptanoid, 1,5-epoxy-3-hydroxy-1-（3-methoxy-4,5-dihydroxyphenyl）-7-（4-hydroxyphenyl）-heptane （1）, as well as a new monoterpene, 10-O-β-n-glucopyranosyl-hydroxy cineole （2） were isolated from the rhizomes of Zingiber officinale. The structures of compounds 1 and 2 were established based on their spectral data. In addition, the antioxidant activities of these compounds were also measured.展开更多
Objective:To develop and validate a simple,accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol inZingiber officinaleextract and gingercontaining dietary supplements,teas an...Objective:To develop and validate a simple,accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol inZingiber officinaleextract and gingercontaining dietary supplements,teas and commercial creams.Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254(E-Merck,Germany)with n-hexane:ethyl acetate 60:40(v/v)as mobile phase.Camag TLC Scanner III was used for the UV densitometric scanning at 569.Results:This system was found to give a compact spot of 8-gingerol at retention factor(Rf) value of(0.39依0.04)and linearity was found in the ranges 50-500 ng/spot(r2=0.9987).Limit of detection(12.76 ng/spot),limit of quantification(26.32 ng/spot),accuracy(less than 2%)and recovery(ranging from 98.22-99.20)were found satisfactory.Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple,accurate,reproducible,sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinaleextract and ginger-containing dietary supplements,teas and commercial creams.展开更多
基金The Guangdong Basic and Applied Basic Research Foundation(2022A1515010730)the National Natural Science Foundation of China(32001647)+2 种基金the Science and Technology Plan Projects of Guangzhou City(202102100009)(the Guangzhou Key Laboratory for Intelligent Sensing and Quality Control of Agricultural Products)National Natural Science Foundation of China(31972022)the 111 Project(B17018)to conduct the project are gratefully acknowledged。
文摘The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The results showed that DOP improved glycolipid metabolism and serum inflammation levels,and inhibited intestinal-derived lipopolysaccharide(LPS)translocation,suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM.Interestingly,the study found that DOP reduced intestinal inflammation and oxidative stress levels,significantly up-regulated the mRNA expression of tight junction proteins Claudin-1,Occludin and zonula occluden-1(ZO-1),and ameliorated intestinal epithelial damage.In addition,DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter,Enterococcus and Desulfovibrio with a reduction rate of 95%,73% and 9%,respectively,and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%,respectively.Taken together,the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier,and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiotamodulatory effects,providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.
基金supported by the Hubei Provincial Natural Science Foundation,China(2022CFB009)the National Natural Science Foundation of China(31900242).
文摘Dendrobium officinale is an orchid herb distinguished by its exceptional drought resistance capabilities.The remarkable drought tolerance of D.officinale stems from its structural and compositional features,including thick leaves and stems containing abundant polysaccharides and colloidal substances.Despite these adaptations,the underlying molecular mechanisms responsible for enhanced drought tolerance remain inadequately understood.This study subjected D.officinale to water restriction for periods ranging from 1 to 6 months,conducting physiological and RNA sequencing analyses to elucidate its long-term dehydration response mechanisms and identify drought-protective genes.Following 6 months of dehydration,D.officinale maintained viability,demonstrated by rapid growth resumption after merely 2 d of rehydration.Transcriptome analysis of D.officinale plants under 1-month dehydration revealed differential gene expression across various processes,predominantly in stress responses,photosynthesis,phytohormone signaling,carbon metabolism,and fructose/mannose pathways.Among these,PEROXIDASE4(POD4)and NAC37 showed significant upregulation and were selected for further investigation of their roles in drought protection.Transgenic tomato plants overexpressing D.officinale’s POD4 and NAC37 genes exhibited superior drought tolerance compared to controls,displaying enhanced vigor,increased fruit production,higher respiration rates,elevated chlorophyll levels,and reduced oxidative damage.This research demonstrates the value of exploring underutilized species for drought-tolerance genes and identifies POD4 and NAC37 as promising candidates for improving drought tolerance through breeding programs.
基金funded by the Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2023E002,CI2024E003)National Natural Science Foundation of China(82304663)+1 种基金the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ16-XRZ-072,ZZ17-YQ-025,ZXKT22052,and ZXKT22060)the National Key Research and Development Program(2022YFC3501803).
文摘Dendrobium officinale(DO)is a well-recognized medicinal and edible plant with a long history of application in traditional medicinal practices across China and Southeast Asia.Recent studies have demonstrated that DOis abundant in diverse bioactive compounds,including polysaccharides(DOP),flavonoids,alkaloids,and bibenzyls thought to exert a range of pharmacological effects,such as anti-tumor and immunomodulatory effects.However,our comprehensive understanding of two key aspects-pharmacological functions and biosynthetic mechanisms-of DO’s major constituents remains limited,especiallywhen considered within the clinical contexts of traditional use.To address this gap,this study reviews DO’s historical applications,clinical effects,and related formulations through an analysis of ancient texts spanning nearly two millennia-with special attention to region-specific traditional medical texts.This provides a historical and empirical foundation for further exploration of its modern pharmacological potential.Given the central role of DOP in DO’s biological activities,this paper further summarizes its therapeutic applications across various diseases and the underlying mechanisms,with special emphasis on structure-activity relationships.This focus is particularly important because the structural characteristics of DOP are highly dependent on extraction and analytical methods,which have contributed to inconsistencies in pharmacological findings over the past two decades.Finally,the review highlights recent advances in the understanding of the in vivo biosynthesis and regulatory mechanisms of themajor bioactive components inDO,with a particular focus onmolecular regulation and responses to agricultural interventions.These factors are critical for the production of high-quality DO.Overall,this study develops a comprehensive knowledge framework that connects DO’s traditional applications of DO to its two key research areas:pharmacological functions and quality formation.We anticipate this framework will offer clear guidance for future research from a clinical perspective.
文摘Background:Skin photoaging is a physiological or pathological process caused by multiple factors.Developing anti-skin photoaging drugs is a hot topic in cosmetology research fields.The purpose of this study was to explore the therapeutic effect of Dendrobium officinale(D.officinale)on skin aging.Methods:The ingredients of D.officinale were detected by UHPLC-Q-TOF/MS.The targets of D.officinale were screened by Swiss Target Prediction database.GeneCards,NCBI,and OMIM databases were utilized to find out the targets associated with skin photoaging.Overlapping targets of D.officinale and skin photoaging were obtained by Venn analysis.The ingredient-disease target network and protein-protein interaction network were constructed by using the STRING database and Cytoscape software.The key compounds and hub genes were obtained by analyzing networks.The DAVID database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the overlapping targets.Autodock Vina software was used to simulate molecular docking and the results were visualized using Pymol.Finally,the skin photoaging models of cells and mice were established to validate the results predicted by network pharmacology.Results:In D.officinale,a total of 59 compounds and 595 targets were detected,of which 59 proteins were intersectional with skin photoaging targets.The top 10 active ingredients(Dendrophenol,Herbacetin,Lyoniresinol,Trans-ferulaldehyde,Naringenin,and so on)and 8 hub genes(AKT1,TNF,VEGFA,MAPK3,CASP3,MMP9,CTNNB1,and EGFR)were identified.All the key active compounds could bind well with core protein targets(binding energy<-5 kcal/mol).The potential therapeutic targets were related to the response to reactive oxygen species,collagen catabolic process,extracellular matrix organization,and apoptotic process,mainly.We also found that D.officinale could enhance the cell viability and activity of anti-oxidases,reduce reactive oxygen species and MMP9 levels,and stable mitochondrial membrane potential.Furthermore,D.officinale could alleviate skin photoaging injury and reduce malondialdehyde level in mice.Conclusion:D.officinale alleviated skin photoaging via regulating oxidative stress,apoptosis,and collagen catabolic process.
基金supported by the National Natural Science Foundation of China(82274134)the National Key R&D Plan(2017YFC1702200,2017YFC1702202)+1 种基金Key R&D Program Projects in Zhejiang Province(2020C04020)the Key Research and Development Program of Zhejiang Province(2025C02183).
文摘The aim of this study was to investigate the role of Dendrobium officinale crude polysaccharides(DOPS)and D.officinale purified polysaccharides(DOPS100)in attenuating immune dysfunction in subfertile mice.The results of the study revealed several noteworthy findings.First,DOPS and DOPS100 treatments led to significant improvement in weight gain and reversal of fatigue-related behaviors compared to the normal group.Second,both DOPS and DOPS100 showed effectiveness in reducing immune organ swelling,modulating immunoglobulin A(IgA)and immunoglobulin M(IgM)levels,and restoring complement(C3c and C4)levels.In addition,they demonstrated a significant ability to enhance the integrity of the intestinal mechanical barrier by differentially upregulating the tight junction proteins Occludin and Zonula occludens 1(ZO-1).In addition,it was found that DOPS100 specifically enhanced the CD4^(+)-T helper 17 cell(Th17)/regulatory T cell(Treg)immune axis in the gut,as evidenced by increased expression of forkhead box protein 3(Foxp3)as well as decreased expression of retinoic acid receptor-related orphan receptor γt(RORγt),and further modulation of interleukin 10(IL-10),interleukin 22(IL-22),and interleukin 17A(IL-17A)expression levels of inflammatory factors.These findings collectively suggest that DOPS100 holds significant potential in improving sub-healthy status by repairing the intestinal barrier,restoring local immune homeostasis,and activating the intestinal immune regulatory network.The study’s outcomes provide valuable insights into the therapeutic implications of DOPS and DOPS100 in addressing immune dysfunction in sub-healthy conditions.
基金Supported by the Scientific Research Project of Neijiang Normal Unverisity(No:07NJZ-03)~~
文摘[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification from mycorrhiza of wild Dendrobium officinale and inoculated on the root system of Dendrobium officinale for inoculation test. [Result] Under tissue-culture conditions, at early stage, Tj1 strain hadn＇t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tj1 strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets; under outdoor conditions, after inoculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [Conclusion] The two inoculation methods both indicate that Tj2 strain has relatively strong promotion effects on the growth of Dendrobium officinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain has practical application values on the successful cultivation of Dendrobium officinale plantlets.
文摘The bioassay was directed to the fractionation of the methanol extract of the rhizomes of Zingiber officinale Roscoe leading to the isolation of a new antioxidant cyclic diarylheptanoid. The structure of the new compound was established as 1, 5-epoxy-3-hydroxy-1-(3,4-dihydroxy-5-methoxy-phenyl)-7-(3,4-dihydroxyphenyl) heptane (1) on the basis of MS, 1D and 2D-NMR experiments.
基金Supported by Fundamental Research Funds for Guangxi Academy of Sciences(13YJ22SWS20)~~
文摘In summer, Cha No. 39 of 292 bags and 205 of 244 bags were inter- planted with Dendrobium officinale on cultivation bed, respectively, whose yields and biological efficiencies reached 102.46 kg and 103.98%, and 96.77 kg and 113.32%, accordingly. The seedlings of Dendrobium officinale interplanted with Pleurotus os- treatus grew by 2.78 cm during 10 weeks on cultivation beds, and the seedlings grew by 2.68 cm in the cultivation beds without interplanting arrangement. These demonstrated that the interplanting of Dendrobium officinale and Pleurotus ostreatus has none adverse effects on growth of Dendrobium officinale.
文摘为了深入研究豆瓣菜(Nasturtium officinale R. Br.)对锌和铁的吸收和转运,我们采用RACE方法克隆了一种豆瓣菜根部锌铁转运蛋白NoZIP2 (N. officinale zinc iron transporter protein 2)的全长cDNA,并采用生物信息学方法对序列进行分析。结果表明,NoZIP2包含1 446 bp,编码一个357个氨基酸残基的多肽,包含了9个跨膜结构域,存在植物锌铁转运蛋白的保守结构域,可能参与了豆瓣菜锌铁转运的过程。NoZIP2氨基酸序列与其它物种ZIP的氨基酸序列相似性较高,其中与荠菜的相似性最高,氨基酸相似性高达92%。分子进化分析表明,NoZIP2与十字花科植物荠菜、荠蓝和小花蓝芥的ZIP能够聚到一起。从豆瓣菜根部克隆的NoZIP2具有其它植物锌铁转运蛋白相似的高度保守的ZIP家族成员序列特征,因此推测NoZIP2属于ZIP家族成员。豆瓣菜No ZIP2的克隆和序列分析能够为进一步研究其功能及表达提供理论基础。
基金Supported by Science and Technology Project of Chongqing Municipal Education Commission(KJ121216)Achievement Transformation Project of Chongqing Municipal Education Commission(KJZH14216)~~
文摘[Objective] This study was conducted to provide scientific basis for fertilization of ginger with high quality and high yield. [Method] The soil nutrient contents and plant nutrient characteristics of Zhugen ginger（Zingiber officinale Rosc.） were studied. [Result] The results showed that： the soil of ginger planting area in Chongqing（Yongchuan） Seedlings of science and technology city was acidic overall,the soil organic matter content was low and alkali-hydrolyzable N was at moderate level; and the contents of available P, Ca, Mg, Fe, Mn, Cu and Zn were extremely rich, while the available S was in lack. The contents of N and K were abundant and P was little in ginger plants, the content of K was more than N in ginger roots and stems, while the content of N was more than K in ginger leaves. [Conclusion]In practical production, it was necessary to fertilize rationally and control the ratio of N fertilizer to K fertilizer according to local conditions, thereby promoting ginger growth and realize high-yield ginger production.
基金financrally supported by K.S.Rangasamy College of Arts and Science.Kuchipalayam,Tiruchengodr.Tamil Nadu,India(grant No.KSRCAS/PG/MB/0010 dt.10.11.2010)
文摘Objective:To evaluate the antibacterial properties ot Allium sativum(garlic) cloves and Zingiber officinale(ginger) rhizomes against multi-drug resistant clinical pathogens causing nosocomial infection.Methods:The cloves of garlic and rhizomes of ginger were extracted with 95%(v/v) ethanol.The ethanolic extracts were subjected to antibacterial sensitivity test against clinical pathogens.Results:Anti-bacterial potentials of the extracts of two crude garlic cloves and ginger rhizomes were tested against five gram negative and two gram positive multi-drug resistant bacteria isolates.All the bacterial isolates were susceptible to crude extracts of both plants extracts.Except Enterobacter sp.and Klebsiella sp.,all other isolates were susceptible when subjected to ethanolic extracts of garlic and ginger.The highest inhibition zone was observed with garlic(19.4S mm) against Pseudomonas aeruginosa(P.aeruginosa).The minimal inhibitory concentration was as low as 67.00 μg/mL against P.aeruginosa.Conclusions:Natural spices of garlic and ginger possess effective anti-bacterial activity against multi-drug clinical pathogens and can be used for prevention of drug resistant microbial diseases and further evaluation is necessary.
基金supported by the Opening Project of State Key Laboratory of Quality Research in Chinese Medicine(Macao University of Science and Technology)(No.MUST-SKL-2016-07)the National Training Program of Innovation and Entrepreneurship for Undergraduates(No.201710572024)
文摘Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usually a difficult task. In this study,we developed a rapid identification protocol for D. officinale using the loop-mediated isothermal amplification(LAMP) method. A set of primers were specifically designed to detect a modified internal transcribed spacer region of D. officinale at 65 ℃ within 40 min after adding SYBR~? Green I, which was used for the detection of D. officinale. Unlike commonly used adulterants, reaction mixtures containing D. officinale DNA changed from orange to green, and this color change was easily observed with the naked eye. Thus, this methodology can be used to accurately differentiate D. officinale from other Dendrobium species, is quick as all D. officinale samples were amplified within 40 min, and specific as samples of the adulterants were not amplified. The specificity of this LAMP-based method was confirmed by testing 17 samples of D. officinale and 32 adulterant samples from other Dendrobium species. This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.
文摘OBJECTIVE： The present study is aimed at evaluating the protective effects of oils from Zingiber officinale （ginger） and Curcuma Ionga （turmeric） on acute ethanol-induced fatty liver in male Wistar rats. METHODS： Ferric reducing antioxidant power activity and oxygen radical absorbance capacity of the oils were evaluated ex vivo. Rats were pretreated for 28 d with standard drug （Livolin Forte） and oils from Z. officinale and C. Ionga before they were exposed to 45% ethanol （4.8 g/kg） to induce acute fatty liver. Histological changes were observed and the degree of protection was measured by using biochemical parameters such as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities. Serum triglyceride （TG） level, total cholesterol （TC） level and the effects of both oils on reduced gluthatione （GSH）, glutathione-S- transferase （GST）, superoxide dismutase （SOD） and hepatic malondialdehyde （MDA） levels were estimated. RESULTS： Oils from Z. officinale and C. Ionga at a dose of 200 mg/kg showed hepatoprotection by decreasing the activities of serum enzymes, serum TG, serum TC and hepatic MDA, while they significantly restored the level of GSH as well as GST and SOD activities. Histological examination of rats tissues was related to the obtained results. CONCLUSION： From the results it may be concluded that oils from Z. officinale and C. Ionga （200 mg/kg） exhibited hepatoprotective activity in acute ethanol-induced fatty liver and Z. officinale oil was identified to have better effects than C. Ionga oil.
基金projects of the Science and Technology Department of Zhejiang Province of China(Nos.2018C02048,2018F10076)。
文摘Dendrobium officinale Kimura et Migo(D. officinale) is a famous traditional Chinese medicine(TCM). A mixture of D. officinale and American ginseng has been shown to enhance cell-mediated immunity, humoral immunity, and monocyte/macrophage functions in mice. Here, the effects of a D. officinale and American ginseng mixture on the structure of gut microbial community in dogs were examined using high-throughput 16S rRNA gene amplicon sequencing. The data revealed that while the mixture did not change the diversity of gut microbial community significantly, differences among individuals were significantly reduced. Furthermore, the mixture-responsive operational taxonomic units(OTUs) exhibited a phase-dependent expression pattern. Fifty-five OTUs were found to exhibit a mixture-induced expression pattern, among which one third were short-chain fatty acid(SCFA)-producing genera and the others were probiotic genera included Lactobacillus spp., Sutterella, Alistipes, Anaerovorax, Bilophila, Coprococcus, Gordonibacter, Oscillibacter, among others. By contrast, 36% of the OTUs exhibiting a mixture-repressed expression pattern were disease-associated microorganisms, and six genera, namely Actinomyces, Escherichia/Shigella, Fusobacterium, Slackia, Streptococcus and Solobacterium, were associated with cancer. In addition, five genera were closely associated with diabetes, namely Collinsella,Rothia, Howardella, Slackia and Intestinibacter. Our results indicate that this D. officinale and American ginseng mixture may be used as a prebiotic agent to enhance SCFA-producing genera and prevent gut dysbiosis.
基金Supported by National Natural Science Foundation of China(81060028)Key Project of Science and Technology of Guangxi Zhuang Autonomous Region(Gui Ke Zhong 1598005-9)Key Science and Technology Program of Guangxi Zhuang Autonomous Region(Gui Ke Gong 1355005-3-7)
文摘Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient content and high cost,limiting the use of D. officinale.Studying the network structure and rate-limiting steps of secondary metabolites of medicinal components of D. officinale,analyzing the secondary metabolic synthesis process,mastering the production rules of its medicinal components and carrying out gene cloning or biosynthesis,etc.are of great significance for the rational development and utilization of D. officinale resources. This paper briefly reviews the progress of the research on the secondary metabolites of D. officinale,including the detection and identification of metabolites and the identification and cloning of key metabolic enzymes.
文摘Aim: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. Methods: The aqueous extracts of the two plants were gavaged separately to 2 groups of rats at a similar dose of 600 mg.kg^1.day-1 for 8 days. At the end of the treatment, the animals were killed and the blood, testis, epididymis, seminal vesicles and prostate were collected for biochemical analysis. Results: The aqueous extract of Z. officinale significantly increased in the relative weight of the testis, the serum testosterone level, testicular cholesterol level and epididymal α-glucosidase activity. The aqueous extract of P. brazzeana significantly increased the weights of the testis, seminal vesicles and prostate. It also significantly increased the serum and testicular testosterone level. The fructose, α-glucosidase and cholesterol levels in P. brazzeanatreated rats were increased by 28%, 35% and 114%, respectively. Conclusion: The aqueous extracts of both P. brazzeana and Z. officinale have an androgenic activity, which seems to be more potent with P. brazzeana than with Z. officinale. (Asian J Androl 2002 Dec; 4: 299-301)
文摘A new cyclic diarylheptanoid, 1,5-epoxy-3-hydroxy-1-（3-methoxy-4,5-dihydroxyphenyl）-7-（4-hydroxyphenyl）-heptane （1）, as well as a new monoterpene, 10-O-β-n-glucopyranosyl-hydroxy cineole （2） were isolated from the rhizomes of Zingiber officinale. The structures of compounds 1 and 2 were established based on their spectral data. In addition, the antioxidant activities of these compounds were also measured.
基金Supported by Deanship of Scientific Research,Salman B in Abdulaziz University,Al-kharj,KSA(Grant No.33/S/54)
文摘Objective:To develop and validate a simple,accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol inZingiber officinaleextract and gingercontaining dietary supplements,teas and commercial creams.Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254(E-Merck,Germany)with n-hexane:ethyl acetate 60:40(v/v)as mobile phase.Camag TLC Scanner III was used for the UV densitometric scanning at 569.Results:This system was found to give a compact spot of 8-gingerol at retention factor(Rf) value of(0.39依0.04)and linearity was found in the ranges 50-500 ng/spot(r2=0.9987).Limit of detection(12.76 ng/spot),limit of quantification(26.32 ng/spot),accuracy(less than 2%)and recovery(ranging from 98.22-99.20)were found satisfactory.Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple,accurate,reproducible,sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinaleextract and ginger-containing dietary supplements,teas and commercial creams.
