[Objectives]To compare the effects of traditional processing and fresh processing on the quality of Polygonatum odoratum decoction piece.[Methods]The effects of fresh processing and traditional processing on the quali...[Objectives]To compare the effects of traditional processing and fresh processing on the quality of Polygonatum odoratum decoction piece.[Methods]The effects of fresh processing and traditional processing on the quality of P.odoratum decoction piece were compared and analyzed with appearance characteristics,total ash content,extract content,total polysaccharides content,and total flavonoids content as the evaluation indexes.[Results]Fresh processing method in different production areas has different effects on P.odoratum decoction piece.P.odoratum was dried in oven of 50℃.When moisture content was 41.44%-59.67%,it was cut.After complete drying at 50℃,the moisture content of dried P.odoratum was 8.94%-9.60%,and ethanol-soluble extract content was 77.29%-78.20%,and water-soluble extract was 77.7%-78.14%.At this time,the appearance characteristics of section of P.odoratum decoction piece were better than that of traditional processing,which was yellowish white.The total polysaccharide content was higher than that of traditional processing,and the content of total flavonoids was statistically significant different from that of traditional processing.[Conclusions]The quality of P.odoratum decoction piece by fresh processing is better than that of the traditional processing,and it is feasible to use fresh processing.展开更多
Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-...Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-5,7,8-trihydroxy-3-(4- hydroxybenzyl)-6-methylchroman-4-one(2),on the basis of spectral analysis.展开更多
A new furostanol component glycosylated only at C-26 was isolated from the rhizomes of Polygonatum odoratum (Mill.) Druce, and its structure was characterized as 22-hydroxy-25(R and S) furost-5-en-12-on-3b, 22, 26-tri...A new furostanol component glycosylated only at C-26 was isolated from the rhizomes of Polygonatum odoratum (Mill.) Druce, and its structure was characterized as 22-hydroxy-25(R and S) furost-5-en-12-on-3b, 22, 26-triol 26-O-b-D-glucopyranoside on the basis of spectroscopic techniques and chemical methods.展开更多
Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and t...Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis(2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line(HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis. Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured against Staphylococcus epidermidis, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells(HL-60), with an IC_(50) of(350.00±1.85) μg/mL for 48 h and(38.00±0.92) μg/mL for 72 h. Additionally, HL-60 became apoptotic and accumulated in G_1-phase after 48 hours of treatment. Conclusions: The extracts of Polygonum odoratum exhibit potential antihemolytic activity. They also have antibacterial activity by inhibiting growth of Gram-positive bacteria. The leaf extract shows anti-cancer activity against HL-60 to a greater extent than the stem extract, causing decreased viability, increased G_1-phase accumulation and apoptosis induction.展开更多
Phytochemical investigation of Sanrafaelia ruffonammari Verd and Ophrypetalum odoratum Diels that belongs to the rare genera confined to East African coastal forests led to the isolation of enantiomeric styrylpyrone d...Phytochemical investigation of Sanrafaelia ruffonammari Verd and Ophrypetalum odoratum Diels that belongs to the rare genera confined to East African coastal forests led to the isolation of enantiomeric styrylpyrone dimer,(±)-5-methoxy-7-phenyl-[4-methoxy-2-pyronyl]-1-(E)-styryl-2-oxabicyclo-[4.2.0]-octa-4-en-3-one(1)alongside(?)-6-styryl-7,8-epoxy-4-methoxypyran-2-one(2)and the enantiomeric(?)-(3)and(-)-6-styryl-7,8-dihydroxy-4-methoxypyran-2-ones(4).Their structures were established by means of spectroscopic methods.In this paper we reveal for the first time the occurrence of styrylpyrones in East African biodiversity.(?)-6-Styryl-7,8-epoxy-4-methoxypyran-2-one(2)and the dihydroxystyrylpyrone enantiomer(3)showed in vitro antifungal activity against Candida albicans at a concentration of 24.4 and 26.2 lM with zones of inhibition of 17 and 9 mm,respectively.Compound 2 exhibited strong activity in the brine shrimp test with LC50=1.7 lg/mL.Their high cytotoxic and antifungal activities render them candidates for further scientific attention for drug development programs against cancer and microbial infections.展开更多
This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(...This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(PAOA-flavone)-promoted small ubiquitin-related modifier(SUMO)modification in the atheroprotective efficacy of HNF1b.HNF1b was predicted to be a transcriptional regulator of sortilin expression via bioinformatics,dual-luciferase reporter gene assay,and chromatin immunoprecipitation.HNF1b overexpression decreased sortilin expression and cellular lipid contents in THP-1 macrophages,leading to a depression in atherosclerotic plaque formation in low-density lipoprotein(LDL)receptor-deficient(LDLR−/−)mice.Multiple SUMO1-modified sites were identified on the HNF1b protein and co-immunoprecipitation confirmed its SUMO1 modification.The SUMOylation of HNF1b protein enhanced the HNF1b-inhibited effect on sortilin expression and reduced lipid contents in macrophages.PAOA-flavone treatment promoted SUMO-activating enzyme subunit 1(SAE1)expression and SAE1-catalyzed SUMOylation of the HNF1b protein,which prevented sortilin-mediated lipid accumulation in macrophages and the formation of atherosclerotic plaques in apolipoprotein E-deficient(ApoE−/−)mice.Interference with SAE1 abrogated the improvement in lipid metabolism in macrophage cells and atheroprotective efficacy in vivo upon PAOA-flavone administration.In summary,HNF1b transcriptionally suppressed sortilin expression and macrophage lipid accumulation to inhibit aortic lipid deposition and the development of atherosclerosis.This anti-atherosclerotic effect was enhanced by PAOA-flavone-facilitated,SAE1-catalyzed SUMOylation of the HNF1b protein.展开更多
The allelopathic effects of aqueous extracts of rice husk （Orza sativa L.） and sorghum stem （Sorghum bicolor L.） on the germination and seedling growth of Chromolaena odoratum L. were investigated. The extracts of...The allelopathic effects of aqueous extracts of rice husk （Orza sativa L.） and sorghum stem （Sorghum bicolor L.） on the germination and seedling growth of Chromolaena odoratum L. were investigated. The extracts of the two crop residues exhibited inhibitory effects on the germination and seedling growth of Chromolaena odoratum L. The degree of retardation demonstrated in both extracts was concentration dependent. However the results obtained revealed that the retardation was more pronounced in the rice husk extract-treated seeds. However statistical analysis （P 〈 0.05% level） revealed that the degrees of inhibition of radicle and plumule obtained from various extracts treated seeds of both extracts were not significantly different when compared to those obtained from the control experiments as well as those obtained from the varying extract concentrations.展开更多
The invasion pattern of Eupatorium odoratum in invasion area was studied by random plot sampling method. Eighteen habitat factors of E. odoratum sampling plots such as altitude, gradient and exposure were quantified t...The invasion pattern of Eupatorium odoratum in invasion area was studied by random plot sampling method. Eighteen habitat factors of E. odoratum sampling plots such as altitude, gradient and exposure were quantified to precisely describe the invasion patterns of E. odoratum in various habitats with different characteristics, and carried out principal component analysis （PCA）. The results showed that the ecological information of 18 habitat factors of E. odoratum could be characterized by four comprehensive factors. The occurrence frequency of E. odoratum had positive eorrelation with the ecological factors such as exposure, soil moisture, total number of plants, number of shrubs, weeds height, distance form water source, farmland distance, air humidity, arbor height, arbor size, altitude, gradient, number of plant species and weeds coverage, which had negative correlation with air temperature.展开更多
This paper mainly studies the effects of different propagation materials and cultivation years on the yield of Polygonatum odoratum under the forest.In this study,the cultivation experiment of P.odoratum was carried o...This paper mainly studies the effects of different propagation materials and cultivation years on the yield of Polygonatum odoratum under the forest.In this study,the cultivation experiment of P.odoratum was carried out by using the stem tip,stem body and seeds of P.odoratum as propagation materials.The yield of P.odoratum in different cultivation years with propagation by rhizome was also investigated.That is,the continuous growth of P.odoratum and the yield of P.odoratum each year after cultivation was statistically analyzed.The results showed that the germination rate under stem tip cultivation was fast,and the emergence time was also fast,which was more than 2 weeks earlier than that under stem body cultivation,and the seedling emergence rate reached 100%.The stem body was a new plant formed by sprouting from the buds of internodes,and the emergence rate was more than 95%.P.odoratum propagated by rhizome was harvested in the third or fourth year after cultivation,and the third year was the best harvest time.展开更多
[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty hea...[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty healthy male Sprague-Dawley(SD)rats were randomly divided into two groups according to their body weight:10 in normal control group(Group NC,n=10)and 20 in experimental group(n=20).The rats in experimental group were fed a high-fat diet for eight weeks before they were further randomly divided into two groups:high fat group(Group HF)and high fat+400 mg/(kg·d)POP group(Group HF+POP).In Group HF+POP,the rats were administered with POP for another six weeks,before their blood plasma was collected,and the relative weights of their testis and epididymis were calculated.The plasma levels of testosterone(T),estrogen(E2),follicle-stimulating hormone(FSH),cortisol(C)and luteinizing hormone(LH)were measured by radioimmunoassay,and the plasma levels of sex hormone-binding globulin(SHBG)and insulin-like growth factor-1(IGF-1)were determined by enzyme-linked immunosorbent assay.[Results]Compared with Group HF,POP could effectively inhibit rat obesity caused by high-fat diets,increase the relative weights of their testis and epididymis,plasma levels of LH,E2,FSH,T,SHBG and IGF-1,and reduce the plasma level of E2.[Conclusions]Polygonatum odoratum polysaccharide(POP)is able to effectively regulate the level of reproductive hormones in high-fat diet fed rats,and helps to protect their reproductive function.展开更多
[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different conce...[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars.展开更多
[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-cr...[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum.展开更多
目的梳理国内外相关研究,通过对玉竹多糖年度发文量、研究机构、发文作者、关键词的可视化知识图谱进行分析,揭示药食同源两用药材玉竹多糖类成分的研究前沿热点。方法采用CiteSpace 6.3.R1软件分析中国知网及Web of Science等数据库200...目的梳理国内外相关研究,通过对玉竹多糖年度发文量、研究机构、发文作者、关键词的可视化知识图谱进行分析,揭示药食同源两用药材玉竹多糖类成分的研究前沿热点。方法采用CiteSpace 6.3.R1软件分析中国知网及Web of Science等数据库2004年1月1日至2024年8月1日国内外与玉竹多糖相关的文献。按照纳入和排除标准进行筛选,对文献进行可视化分析。结果经筛选,最终纳入文献441篇,含中文文献292篇,英文文献149篇。相关发文量呈现一定程度波浪式增长。关键词分析显示,国内玉竹多糖的研究热点为提取工艺及药理作用,而国外更侧重于玉竹多糖的药理机制研究。结论玉竹及其多糖的研究热点集中在化学成分、结构表征以及抗氧化、氧化应激、糖尿病等药理活性和作用机制的研究,这可能是未来该领域的研究趋势。展开更多
基金Supported by Guangxi Science and Technology Major Project(GUIKE AA22096020)Central Guidance for Local Scientific and Technological Development Funds(ZY20230102)+2 种基金Guilin City Science Research and Technology Development Plan Project(20220104-4,20210202-1,2020011203-1,2020011203-2)Open Project of Guangxi Key Laboratory of Tumor Immunology and Microenvironment Regulation(2022KF005)College Students Innovative Entrepreneurial Training Plan Program(202210601015).
文摘[Objectives]To compare the effects of traditional processing and fresh processing on the quality of Polygonatum odoratum decoction piece.[Methods]The effects of fresh processing and traditional processing on the quality of P.odoratum decoction piece were compared and analyzed with appearance characteristics,total ash content,extract content,total polysaccharides content,and total flavonoids content as the evaluation indexes.[Results]Fresh processing method in different production areas has different effects on P.odoratum decoction piece.P.odoratum was dried in oven of 50℃.When moisture content was 41.44%-59.67%,it was cut.After complete drying at 50℃,the moisture content of dried P.odoratum was 8.94%-9.60%,and ethanol-soluble extract content was 77.29%-78.20%,and water-soluble extract was 77.7%-78.14%.At this time,the appearance characteristics of section of P.odoratum decoction piece were better than that of traditional processing,which was yellowish white.The total polysaccharide content was higher than that of traditional processing,and the content of total flavonoids was statistically significant different from that of traditional processing.[Conclusions]The quality of P.odoratum decoction piece by fresh processing is better than that of the traditional processing,and it is feasible to use fresh processing.
文摘Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-5,7,8-trihydroxy-3-(4- hydroxybenzyl)-6-methylchroman-4-one(2),on the basis of spectral analysis.
基金Project 02-03zp09 was supported by the Science Foundation of State Administration of Traditional Chinese Medicine,P.R.China
文摘A new furostanol component glycosylated only at C-26 was isolated from the rhizomes of Polygonatum odoratum (Mill.) Druce, and its structure was characterized as 22-hydroxy-25(R and S) furost-5-en-12-on-3b, 22, 26-triol 26-O-b-D-glucopyranoside on the basis of spectroscopic techniques and chemical methods.
基金financially supported by Mae Fah Luang University(No.59111040017)
文摘Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis(2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line(HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis. Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured against Staphylococcus epidermidis, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells(HL-60), with an IC_(50) of(350.00±1.85) μg/mL for 48 h and(38.00±0.92) μg/mL for 72 h. Additionally, HL-60 became apoptotic and accumulated in G_1-phase after 48 hours of treatment. Conclusions: The extracts of Polygonum odoratum exhibit potential antihemolytic activity. They also have antibacterial activity by inhibiting growth of Gram-positive bacteria. The leaf extract shows anti-cancer activity against HL-60 to a greater extent than the stem extract, causing decreased viability, increased G_1-phase accumulation and apoptosis induction.
基金This work was financially supported by the Norwegian Agency for International Development(NORAD)through the NORAD Chemistry Project.
文摘Phytochemical investigation of Sanrafaelia ruffonammari Verd and Ophrypetalum odoratum Diels that belongs to the rare genera confined to East African coastal forests led to the isolation of enantiomeric styrylpyrone dimer,(±)-5-methoxy-7-phenyl-[4-methoxy-2-pyronyl]-1-(E)-styryl-2-oxabicyclo-[4.2.0]-octa-4-en-3-one(1)alongside(?)-6-styryl-7,8-epoxy-4-methoxypyran-2-one(2)and the enantiomeric(?)-(3)and(-)-6-styryl-7,8-dihydroxy-4-methoxypyran-2-ones(4).Their structures were established by means of spectroscopic methods.In this paper we reveal for the first time the occurrence of styrylpyrones in East African biodiversity.(?)-6-Styryl-7,8-epoxy-4-methoxypyran-2-one(2)and the dihydroxystyrylpyrone enantiomer(3)showed in vitro antifungal activity against Candida albicans at a concentration of 24.4 and 26.2 lM with zones of inhibition of 17 and 9 mm,respectively.Compound 2 exhibited strong activity in the brine shrimp test with LC50=1.7 lg/mL.Their high cytotoxic and antifungal activities render them candidates for further scientific attention for drug development programs against cancer and microbial infections.
基金supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region,China (No.2019JJA140728)the Horizontal Cooperation Project with Hunan Mingshun Pharmaceutical Co.,Ltd. (No.2021GLHX02)the Guangxi Province Postgraduate Co-training Base for Cooperative Innovation in Basic Medicine of Guilin Medical University (No.Gui Xue Wei[2020]7).
文摘This study aims to investigate the impact of hepatocyte nuclear factor 1β(HNF1b)on macrophage sortilin-mediated lipid metabolism and aortic atherosclerosis and explore the role of the flavone of Polygonatum odoratum(PAOA-flavone)-promoted small ubiquitin-related modifier(SUMO)modification in the atheroprotective efficacy of HNF1b.HNF1b was predicted to be a transcriptional regulator of sortilin expression via bioinformatics,dual-luciferase reporter gene assay,and chromatin immunoprecipitation.HNF1b overexpression decreased sortilin expression and cellular lipid contents in THP-1 macrophages,leading to a depression in atherosclerotic plaque formation in low-density lipoprotein(LDL)receptor-deficient(LDLR−/−)mice.Multiple SUMO1-modified sites were identified on the HNF1b protein and co-immunoprecipitation confirmed its SUMO1 modification.The SUMOylation of HNF1b protein enhanced the HNF1b-inhibited effect on sortilin expression and reduced lipid contents in macrophages.PAOA-flavone treatment promoted SUMO-activating enzyme subunit 1(SAE1)expression and SAE1-catalyzed SUMOylation of the HNF1b protein,which prevented sortilin-mediated lipid accumulation in macrophages and the formation of atherosclerotic plaques in apolipoprotein E-deficient(ApoE−/−)mice.Interference with SAE1 abrogated the improvement in lipid metabolism in macrophage cells and atheroprotective efficacy in vivo upon PAOA-flavone administration.In summary,HNF1b transcriptionally suppressed sortilin expression and macrophage lipid accumulation to inhibit aortic lipid deposition and the development of atherosclerosis.This anti-atherosclerotic effect was enhanced by PAOA-flavone-facilitated,SAE1-catalyzed SUMOylation of the HNF1b protein.
文摘The allelopathic effects of aqueous extracts of rice husk （Orza sativa L.） and sorghum stem （Sorghum bicolor L.） on the germination and seedling growth of Chromolaena odoratum L. were investigated. The extracts of the two crop residues exhibited inhibitory effects on the germination and seedling growth of Chromolaena odoratum L. The degree of retardation demonstrated in both extracts was concentration dependent. However the results obtained revealed that the retardation was more pronounced in the rice husk extract-treated seeds. However statistical analysis （P 〈 0.05% level） revealed that the degrees of inhibition of radicle and plumule obtained from various extracts treated seeds of both extracts were not significantly different when compared to those obtained from the control experiments as well as those obtained from the varying extract concentrations.
基金Supported by Scientific Research Program of Guangxi Provincial Department of Education ( 201103YB128,201010lx500)Key Scientific Research Project of Baise University ( 2012KA02)+1 种基金Integrated Construction Program of Characteristic Biotechnology Majors and Courses ( GXTSZY224 )2011 Young Teachers Growth Foundation of Baise University
文摘The invasion pattern of Eupatorium odoratum in invasion area was studied by random plot sampling method. Eighteen habitat factors of E. odoratum sampling plots such as altitude, gradient and exposure were quantified to precisely describe the invasion patterns of E. odoratum in various habitats with different characteristics, and carried out principal component analysis （PCA）. The results showed that the ecological information of 18 habitat factors of E. odoratum could be characterized by four comprehensive factors. The occurrence frequency of E. odoratum had positive eorrelation with the ecological factors such as exposure, soil moisture, total number of plants, number of shrubs, weeds height, distance form water source, farmland distance, air humidity, arbor height, arbor size, altitude, gradient, number of plant species and weeds coverage, which had negative correlation with air temperature.
基金Special Project for Central Guidance in Local Science and Technology Development(ZY19C11)Special Project for the Investigation of Basic Resources of Science and Technology(2017FY101302-5)。
文摘This paper mainly studies the effects of different propagation materials and cultivation years on the yield of Polygonatum odoratum under the forest.In this study,the cultivation experiment of P.odoratum was carried out by using the stem tip,stem body and seeds of P.odoratum as propagation materials.The yield of P.odoratum in different cultivation years with propagation by rhizome was also investigated.That is,the continuous growth of P.odoratum and the yield of P.odoratum each year after cultivation was statistically analyzed.The results showed that the germination rate under stem tip cultivation was fast,and the emergence time was also fast,which was more than 2 weeks earlier than that under stem body cultivation,and the seedling emergence rate reached 100%.The stem body was a new plant formed by sprouting from the buds of internodes,and the emergence rate was more than 95%.P.odoratum propagated by rhizome was harvested in the third or fourth year after cultivation,and the third year was the best harvest time.
基金Supported by Scientific Research Fund of the Hunan Provincial Education Department of China(19A259)Natural Science Foundation of Hunan Province(2022JJ30312)+2 种基金National Innovation Experiment Program for University Students(201910553013)2020 Innovation Experiment Program for College Students of Hunan University of HumanitiesScience and Technology(2020-17)。
文摘[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty healthy male Sprague-Dawley(SD)rats were randomly divided into two groups according to their body weight:10 in normal control group(Group NC,n=10)and 20 in experimental group(n=20).The rats in experimental group were fed a high-fat diet for eight weeks before they were further randomly divided into two groups:high fat group(Group HF)and high fat+400 mg/(kg·d)POP group(Group HF+POP).In Group HF+POP,the rats were administered with POP for another six weeks,before their blood plasma was collected,and the relative weights of their testis and epididymis were calculated.The plasma levels of testosterone(T),estrogen(E2),follicle-stimulating hormone(FSH),cortisol(C)and luteinizing hormone(LH)were measured by radioimmunoassay,and the plasma levels of sex hormone-binding globulin(SHBG)and insulin-like growth factor-1(IGF-1)were determined by enzyme-linked immunosorbent assay.[Results]Compared with Group HF,POP could effectively inhibit rat obesity caused by high-fat diets,increase the relative weights of their testis and epididymis,plasma levels of LH,E2,FSH,T,SHBG and IGF-1,and reduce the plasma level of E2.[Conclusions]Polygonatum odoratum polysaccharide(POP)is able to effectively regulate the level of reproductive hormones in high-fat diet fed rats,and helps to protect their reproductive function.
基金Supported by General Project of Natural Science Foundation of Hunan Province (2022JJ30312)Enterprise Science and Technology Innovation Team Construction Project of Loudi Science and Technology Innovation Program in 2022.
文摘[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars.
文摘[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum.
文摘目的梳理国内外相关研究,通过对玉竹多糖年度发文量、研究机构、发文作者、关键词的可视化知识图谱进行分析,揭示药食同源两用药材玉竹多糖类成分的研究前沿热点。方法采用CiteSpace 6.3.R1软件分析中国知网及Web of Science等数据库2004年1月1日至2024年8月1日国内外与玉竹多糖相关的文献。按照纳入和排除标准进行筛选,对文献进行可视化分析。结果经筛选,最终纳入文献441篇,含中文文献292篇,英文文献149篇。相关发文量呈现一定程度波浪式增长。关键词分析显示,国内玉竹多糖的研究热点为提取工艺及药理作用,而国外更侧重于玉竹多糖的药理机制研究。结论玉竹及其多糖的研究热点集中在化学成分、结构表征以及抗氧化、氧化应激、糖尿病等药理活性和作用机制的研究,这可能是未来该领域的研究趋势。
