Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth...Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.展开更多
Inspired by the profoundly observed odd-even staggering and the inverted parabolic-like shape in charge radii along calcium isotopic chain,the ground state properties of calcium isotopes are investigated by constraini...Inspired by the profoundly observed odd-even staggering and the inverted parabolic-like shape in charge radii along calcium isotopic chain,the ground state properties of calcium isotopes are investigated by constraining the root-mean-square charge radii under the covariant energy density functionals with effective forces NL3 and PK1.In this work,the pairing correlations are tackled by solving the state-dependent Bardeen-Cooper-Schrieffer equations.The calculated results suggest that the binding energies obtained by the radius constraint method have been slightly changed by about 0.2%.But for charge radii,the corresponding results deriving from NL3 and PK1 forces have been increased by about 1.0%and 2.0%,respectively.This means that the charge radius is a more sensitive quantity in the calibrated protocol.Meanwhile,it is found that the reproduced charge radii of calcium isotopes are attributed to the rather strong isospin dependence of effective potential.The odd-even oscillation behavior can also be presented in the proton Fermi energies along calcium isotopic family,but keep opposite trends with respect to the corresponding binding energies and charge radii.As encountered in charge radii,the weakened odd-even oscillation behavior still emerged from the proton Fermi energies at the neutron numbers N=20 and 28 as well,but not in binding energies.展开更多
The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Ti...The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.展开更多
The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
基金National Natural Science Foundation of China,Grant/Award Number:32302826 and 32372961Jilin Provincial Special Project for Health Research Talents,Grant/Award Number:2020SCZ40China Postdoctoral Science Foundation,Grant/Award Number:2023M740623。
文摘Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.
基金supported by the Natural Science Foundation of Ningxia Province,China(No.2024AAC03015)the Open Project of Guangxi Key Laboratory of Nuclear Physics and Nuclear Technology,No.NLK2023-05+9 种基金the Central Government Guidance Funds for Local Scientific and Technological Development,China(No.Guike ZY22096024)the Key Laboratory of Beam Technology of Ministry of Education,China(No.BEAM2024G04)the National Natural Science Foundation of China under Grants No.11705118,No.12175151the Major Project of the GuangDong Basic and Applied Basic Research Foundation(2021B0301030006)NT is grateful for the support of the key research and development project of Ningxia(Grant No.2024BEH04090)the Key Laboratory of Beam Technology of Ministry of Education,China(No.BEAM2024G05)the National Natural Science Foundation of China under Grants No.12275025,No.11975096the Fundamental Research Funds for the Central Universities(2020NTST06)supported by the National Key R&D Program of China under Grant No.2023YFA1606401the National Natural Science Foundation of China under Grants No.12135004,No.11635003,No.11961141004.
文摘Inspired by the profoundly observed odd-even staggering and the inverted parabolic-like shape in charge radii along calcium isotopic chain,the ground state properties of calcium isotopes are investigated by constraining the root-mean-square charge radii under the covariant energy density functionals with effective forces NL3 and PK1.In this work,the pairing correlations are tackled by solving the state-dependent Bardeen-Cooper-Schrieffer equations.The calculated results suggest that the binding energies obtained by the radius constraint method have been slightly changed by about 0.2%.But for charge radii,the corresponding results deriving from NL3 and PK1 forces have been increased by about 1.0%and 2.0%,respectively.This means that the charge radius is a more sensitive quantity in the calibrated protocol.Meanwhile,it is found that the reproduced charge radii of calcium isotopes are attributed to the rather strong isospin dependence of effective potential.The odd-even oscillation behavior can also be presented in the proton Fermi energies along calcium isotopic family,but keep opposite trends with respect to the corresponding binding energies and charge radii.As encountered in charge radii,the weakened odd-even oscillation behavior still emerged from the proton Fermi energies at the neutron numbers N=20 and 28 as well,but not in binding energies.
基金supported by Science and Technology Project of State Grid Corporation Headquarters under Grant 5108-202218280A-2-170-XG(Development and Application of Power Time-Sensitive Network Switching Chip。
文摘The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.
文摘The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
