Fluorescence Anisotropy(FA)is an effective biochemical detection method based on molecular rotations.Graphene oxide(GO)has been extensively used as an FA amplifier.However,the enhancement of FA by GO alone is limited ...Fluorescence Anisotropy(FA)is an effective biochemical detection method based on molecular rotations.Graphene oxide(GO)has been extensively used as an FA amplifier.However,the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA inaccurate.In order to address these problems,an octopus-like DNA nanostructure(ODN)was designed and coupled with GO to enhance the FA together in this work.By mimicking the multi-clawed structure of the octopus,the ODN can be adsorbed on GO tightly,which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN,but also could improve the specificity due to the decrease of the nonspecific interaction in complex samples.Furthermore,ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO,which could improve the accuracy.This method has been applied for the detection of hepatitis B virus DNA(HBV-DNA)in a range of 1-50 nmol/L and the limit of detection(LOD)was 330 pmol/L.In addition,the proposed method has been successfully utilized to detect HBV-DNA in human serum,indicating that this method has a great practical application prospect.展开更多
Underwater self-reconfigurable system(USS),an underwater self-reconfigurable robot consisting of two types of modules,can reform various configurations in real time.Compared with normal underwater robots,its diverse c...Underwater self-reconfigurable system(USS),an underwater self-reconfigurable robot consisting of two types of modules,can reform various configurations in real time.Compared with normal underwater robots,its diverse configurations bring forward good adaptability and more abundant gaits.For the tree-like configurations,we propose a technique for dynamic modeling and configuration description.The eel-like configuration and the octopus-like one are chosen as examples to verify the technique,and some gaits are designed.For the eel-like configuration with the serpentine gait,the technique gives the same simulation results as existing eel-like models.For other cases,simulation results are consistent well with what happens in nature.展开更多
基金supported by the National Natural Science Foundation of China(Nos.21974109,22322409)the Natural Science Foundation of Chongqing(No.CSTB2022NSCQ-MSX1662)the Fundamental Research Funds for the Central Universities(No.XDJK2019TY003)。
文摘Fluorescence Anisotropy(FA)is an effective biochemical detection method based on molecular rotations.Graphene oxide(GO)has been extensively used as an FA amplifier.However,the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA inaccurate.In order to address these problems,an octopus-like DNA nanostructure(ODN)was designed and coupled with GO to enhance the FA together in this work.By mimicking the multi-clawed structure of the octopus,the ODN can be adsorbed on GO tightly,which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN,but also could improve the specificity due to the decrease of the nonspecific interaction in complex samples.Furthermore,ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO,which could improve the accuracy.This method has been applied for the detection of hepatitis B virus DNA(HBV-DNA)in a range of 1-50 nmol/L and the limit of detection(LOD)was 330 pmol/L.In addition,the proposed method has been successfully utilized to detect HBV-DNA in human serum,indicating that this method has a great practical application prospect.
基金the National High Technology Research and Development Program(863)of China(Nos.2007AA09Z215 and 2001AA616090)the National Natural Science Foundation of China(Nos.60104001 and 51009091)
文摘Underwater self-reconfigurable system(USS),an underwater self-reconfigurable robot consisting of two types of modules,can reform various configurations in real time.Compared with normal underwater robots,its diverse configurations bring forward good adaptability and more abundant gaits.For the tree-like configurations,we propose a technique for dynamic modeling and configuration description.The eel-like configuration and the octopus-like one are chosen as examples to verify the technique,and some gaits are designed.For the eel-like configuration with the serpentine gait,the technique gives the same simulation results as existing eel-like models.For other cases,simulation results are consistent well with what happens in nature.
