We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) ...We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) snake venoms. Chemiluminescence (ChL) levels were examined in tissue assays after incubation (at 37 °C for a period of 10 min) with venom for in vitro experiments and in tissue assays isolated of 10 min after venom injection for in vivo experiments. The TBA-test was also performed to confirm the free radical expression. The activities of antioxidant enzymes (such as superoxide dismutase and glutathione peroxidase) in isolated tissues were detected by spectro-photometry. During the in vitro processing chemiluminescence levels of tissue homogenates significantly decreased, while in course of in vivo intoxication the level of ChL was elevated in brain and liver;lipid peroxidation also increased in brain tissue, but there was no significant balance change in other tissues;the activity of superoxide dismutase mainly correlated with changes of free radical balance during intoxication. On the contrary, the activity of glutathione peroxidase showed the reverse tendencies to change. We suggest that free radicals and their oxidative stresses may play a role in the early stage of intoxication causing the so-named “spreading-effect”, which is very characteristic for the venom of vipers.展开更多
Objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines.Methods:The antioxidant radical scaveng...Objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines.Methods:The antioxidant radical scavenging activity of the algal extracts was estimated using the 2,2-diphenyl-1-picrylhydrazyl colorimetric assay.Moreover,the cytotoxic potential of these bioactive extracts was studied against HCT-116 and HeLa cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.Wound healing analysis was adopted to evaluate the antimetastatic effects of protein extracts from both algae.Cell cycle analysis was performed by flow cytometry,and apoptotic bodies were detected using 4′,6-diamidino-2-phenylindole fluorescent staining.Amino acids of hydrolyzed protein extract were separated and identified chromatographically by high performance liquid chromatography.Results:The green algae Ulva lactuca had higher lipid content than Laurencia obtusa,whereas the latter had higher protein content with high antioxidant capacity.Protein extracts had significant dose-and time-dependent cytotoxicity and anti-metastatic activity against HCT-116 cells.Protein b extracts of both algae obtained from the chloroform:hexane solvent lipid-free residue caused morphological changes and induced apoptosis of HCT-116 cells.Further analysis revealed that apoptosis induced upon Laurencia obtusa protein b treatment was triggered via the ROS pathway,causing a significant increase in the sub-G_(1) population.Glycine and arginine(5.94 and 5.47μM,respectively)were the most common amino acids in Laurencia obtusa protein b extract.Conclusions:Proteins of Laurencia obtusa and Ulva lactuca could be considered as an adjuvant nutraceutical source of pro-apoptotic and anti-metastatic biomolecules against colon cancer.展开更多
Caryota obtusa of Arecaceae is a fascinating palm tree native to southwestern China,India,Myanmar,Thailand,Laos and Vietnam.It is an economically important and threatened species and appears as a canopy dominant in so...Caryota obtusa of Arecaceae is a fascinating palm tree native to southwestern China,India,Myanmar,Thailand,Laos and Vietnam.It is an economically important and threatened species and appears as a canopy dominant in some karst areas in Yunnan.We aim to clarify the forest structure,species diversity,population status and regeneration dynamics of C.obtusa in the karst forest ecosystem of Yunnan,China.We established 56 vegetation plots dominated by C.obtusa in 10 counties of southern Yunnan.Based on the plot data,we analyzed the community stratification,floristic composition and C.obtusa’s population structure.We used questionnaires to interview local people and recorded the human activity history in C.obtusa-dominated forests.Caryota obtusa palm forests were distributed on limestone mountain slopes and gullies.There were seven forest community types.The stratification of each community included arborous layer,shrub layer and understory.The communities had rich species composition.For all the plots as a whole of each community type,Shannon–Wiener diversity index of either woody or herbaceous species ranged from 2.1 to 3.8.The diameter at breast height(DBH)-class frequency distribution of C.obtusa was a multimodal type.The regeneration was sporadic and dependent on intermediate natural disturbances.In the current population structure,a number of C.obtusa trees with small DBHs consisted mainly of the forest communities with no or a slight degree of human disturbances.Intensive human activities terribly hindered recruitment of C.obtuse,followed by the medium intensity of human activities.展开更多
Aims: The microglia activity of rat brain following exposure of theMacrovipera lebetina obtusa venom was investi-gated. Methods: Histochemical analysis of brain microcirculatory bed staining by Ca2+ ATPase method for ...Aims: The microglia activity of rat brain following exposure of theMacrovipera lebetina obtusa venom was investi-gated. Methods: Histochemical analysis of brain microcirculatory bed staining by Ca2+ ATPase method for variable doses after intraperitoneal injections given for different time periods was used. The hemorrhagic activity of snake venom metalloproteinases was tested. Toxicological data were calculated using Behrens and Miller-Tainter methods. Surface, size of brain microglial cells (MGCs) and staining intensity were quantified using ImageJ software. Results: The vasodestructive action of the venom resulted in changes in ATPase activity. The intensity of staining of rat brain microcirculatory bed was venom dose-, and time-dependent. Increased activity of MGCs in hemorrhagic loci of different regions of venom affected brain was also demonstrated. Conclusion: The activation of microglia and changes of its form, size, and position strongly correlates with hemorrhage-induced cerebrovascular damage.展开更多
Objective:To study the possible effects of Cerithidea obtusa extract as antidiabetic and to screen the secondary metabolites presence.Methods:Antidiabetic activity of Cerithidea obtusa extract was measured in vitro us...Objective:To study the possible effects of Cerithidea obtusa extract as antidiabetic and to screen the secondary metabolites presence.Methods:Antidiabetic activity of Cerithidea obtusa extract was measured in vitro usingα-glucosidase inhibition method.Whereas,secondary metabolites screening was measured qualitatively.Results:The methanol extract had antidiabetic activity(IC_(50)=36.40 mg/mL).However,the control drug acarbose had significantly higher antidiabetic activity(IC_(50)=0.32 mg/mL).Secondary metabolites screening showed the presence of alkaloids,flavonoids,triterpenoids and saponins.Conclusions:The methanol extract had antidiabetic activity and the presence of alkaloids,flavonoids and triterpenoids might contribute to the activity.展开更多
Often facilitated by human-mediated pathways,aquatic invasive species are a threat to the health and biodiversity of global ecosystems.We present a novel approach incorporating survey data of watercraft movement in a ...Often facilitated by human-mediated pathways,aquatic invasive species are a threat to the health and biodiversity of global ecosystems.We present a novel approach incorporating survey data of watercraft movement in a social network analysis to reconstruct potential pathways of aquatic invasive species spread between lakes.As an example,we use the green alga Nitellopsis obtusa,also known as starry stonewort,an aquatic invasive species affecting the Great Lakes region in the United States and Canada.The movement of algal fragments via human-mediated pathways(i.e.,watercraft)has been hypothesized as the primary driver of starry stonewort invasion.We used survey data collected at boat ramps during the 2013 and 2014 openwater seasons to describe the flow of watercraft from Lake Koronis,where N.obtusa was first detected in Minnesota,to other lakes in the state.Our results suggest that the risk of N.obtusa expansion is not highly constrained by geographic proximity and management efforts should consider highly connected lakes.Estimating human movement via network analysis may help to explain past and future routes of aquatic invasive species infestation between lakes and can improve evidence-based prevention and control efforts.展开更多
文摘We investigated the balance of free radicals in different tissues (liver, heart, brain and muscle) of rats in course of in vivo and in vitro processing by Macrovipera lebetina obtusa (MLO) and Montivipera raddei (MR) snake venoms. Chemiluminescence (ChL) levels were examined in tissue assays after incubation (at 37 °C for a period of 10 min) with venom for in vitro experiments and in tissue assays isolated of 10 min after venom injection for in vivo experiments. The TBA-test was also performed to confirm the free radical expression. The activities of antioxidant enzymes (such as superoxide dismutase and glutathione peroxidase) in isolated tissues were detected by spectro-photometry. During the in vitro processing chemiluminescence levels of tissue homogenates significantly decreased, while in course of in vivo intoxication the level of ChL was elevated in brain and liver;lipid peroxidation also increased in brain tissue, but there was no significant balance change in other tissues;the activity of superoxide dismutase mainly correlated with changes of free radical balance during intoxication. On the contrary, the activity of glutathione peroxidase showed the reverse tendencies to change. We suggest that free radicals and their oxidative stresses may play a role in the early stage of intoxication causing the so-named “spreading-effect”, which is very characteristic for the venom of vipers.
文摘Objective:To investigate the antioxidant and cytotoxic properties of the most active extracts from Ulva lactuca and Laurencia obtusa against colon and cervical cancer cell lines.Methods:The antioxidant radical scavenging activity of the algal extracts was estimated using the 2,2-diphenyl-1-picrylhydrazyl colorimetric assay.Moreover,the cytotoxic potential of these bioactive extracts was studied against HCT-116 and HeLa cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.Wound healing analysis was adopted to evaluate the antimetastatic effects of protein extracts from both algae.Cell cycle analysis was performed by flow cytometry,and apoptotic bodies were detected using 4′,6-diamidino-2-phenylindole fluorescent staining.Amino acids of hydrolyzed protein extract were separated and identified chromatographically by high performance liquid chromatography.Results:The green algae Ulva lactuca had higher lipid content than Laurencia obtusa,whereas the latter had higher protein content with high antioxidant capacity.Protein extracts had significant dose-and time-dependent cytotoxicity and anti-metastatic activity against HCT-116 cells.Protein b extracts of both algae obtained from the chloroform:hexane solvent lipid-free residue caused morphological changes and induced apoptosis of HCT-116 cells.Further analysis revealed that apoptosis induced upon Laurencia obtusa protein b treatment was triggered via the ROS pathway,causing a significant increase in the sub-G_(1) population.Glycine and arginine(5.94 and 5.47μM,respectively)were the most common amino acids in Laurencia obtusa protein b extract.Conclusions:Proteins of Laurencia obtusa and Ulva lactuca could be considered as an adjuvant nutraceutical source of pro-apoptotic and anti-metastatic biomolecules against colon cancer.
基金support from Science and Technology Department of Yunnan University,China(2019YNU002).
文摘Caryota obtusa of Arecaceae is a fascinating palm tree native to southwestern China,India,Myanmar,Thailand,Laos and Vietnam.It is an economically important and threatened species and appears as a canopy dominant in some karst areas in Yunnan.We aim to clarify the forest structure,species diversity,population status and regeneration dynamics of C.obtusa in the karst forest ecosystem of Yunnan,China.We established 56 vegetation plots dominated by C.obtusa in 10 counties of southern Yunnan.Based on the plot data,we analyzed the community stratification,floristic composition and C.obtusa’s population structure.We used questionnaires to interview local people and recorded the human activity history in C.obtusa-dominated forests.Caryota obtusa palm forests were distributed on limestone mountain slopes and gullies.There were seven forest community types.The stratification of each community included arborous layer,shrub layer and understory.The communities had rich species composition.For all the plots as a whole of each community type,Shannon–Wiener diversity index of either woody or herbaceous species ranged from 2.1 to 3.8.The diameter at breast height(DBH)-class frequency distribution of C.obtusa was a multimodal type.The regeneration was sporadic and dependent on intermediate natural disturbances.In the current population structure,a number of C.obtusa trees with small DBHs consisted mainly of the forest communities with no or a slight degree of human disturbances.Intensive human activities terribly hindered recruitment of C.obtuse,followed by the medium intensity of human activities.
文摘Aims: The microglia activity of rat brain following exposure of theMacrovipera lebetina obtusa venom was investi-gated. Methods: Histochemical analysis of brain microcirculatory bed staining by Ca2+ ATPase method for variable doses after intraperitoneal injections given for different time periods was used. The hemorrhagic activity of snake venom metalloproteinases was tested. Toxicological data were calculated using Behrens and Miller-Tainter methods. Surface, size of brain microglial cells (MGCs) and staining intensity were quantified using ImageJ software. Results: The vasodestructive action of the venom resulted in changes in ATPase activity. The intensity of staining of rat brain microcirculatory bed was venom dose-, and time-dependent. Increased activity of MGCs in hemorrhagic loci of different regions of venom affected brain was also demonstrated. Conclusion: The activation of microglia and changes of its form, size, and position strongly correlates with hemorrhage-induced cerebrovascular damage.
基金Supported by Directorate of Research and Community Service,Directorate General of Higher Education(DIKTI),Ministry of Education and Culture of Indonesia.
文摘Objective:To study the possible effects of Cerithidea obtusa extract as antidiabetic and to screen the secondary metabolites presence.Methods:Antidiabetic activity of Cerithidea obtusa extract was measured in vitro usingα-glucosidase inhibition method.Whereas,secondary metabolites screening was measured qualitatively.Results:The methanol extract had antidiabetic activity(IC_(50)=36.40 mg/mL).However,the control drug acarbose had significantly higher antidiabetic activity(IC_(50)=0.32 mg/mL).Secondary metabolites screening showed the presence of alkaloids,flavonoids,triterpenoids and saponins.Conclusions:The methanol extract had antidiabetic activity and the presence of alkaloids,flavonoids and triterpenoids might contribute to the activity.
文摘Often facilitated by human-mediated pathways,aquatic invasive species are a threat to the health and biodiversity of global ecosystems.We present a novel approach incorporating survey data of watercraft movement in a social network analysis to reconstruct potential pathways of aquatic invasive species spread between lakes.As an example,we use the green alga Nitellopsis obtusa,also known as starry stonewort,an aquatic invasive species affecting the Great Lakes region in the United States and Canada.The movement of algal fragments via human-mediated pathways(i.e.,watercraft)has been hypothesized as the primary driver of starry stonewort invasion.We used survey data collected at boat ramps during the 2013 and 2014 openwater seasons to describe the flow of watercraft from Lake Koronis,where N.obtusa was first detected in Minnesota,to other lakes in the state.Our results suggest that the risk of N.obtusa expansion is not highly constrained by geographic proximity and management efforts should consider highly connected lakes.Estimating human movement via network analysis may help to explain past and future routes of aquatic invasive species infestation between lakes and can improve evidence-based prevention and control efforts.
