The development of fluorescent probes enabling to distinguish Cys,Hcy and GSH has always been a considerable challenge,in particular the distinction of Hcy and other two biothiols,because Hcy has a very similar struct...The development of fluorescent probes enabling to distinguish Cys,Hcy and GSH has always been a considerable challenge,in particular the distinction of Hcy and other two biothiols,because Hcy has a very similar structure with Cys and a relatively lower concentration in living organisms.In this work,a special o-dialdehyde fluorescent probe,quinoline-2,3-dicarboxaldehyde(QDA),has been synthesized and demonstrated superior performance in differentiating detection of Hcy and GSH,which is different from the previous reported o-dialdehyde probes specifically detecting GSH.Furthermore,the probe can selectively distinguish Hcy and GSH from different signal channels in living cells and zebrafish,meaning it has great potential in biological applications.This finding will provide a novel idea for the design of fluorescent probes to distinguish biothiols.展开更多
基金the National Natural Science Foundation of China(Nos.21672131,21775096,21907062)One Hundred People Plan of Shanxi Province+10 种基金Shanxi Province"1331 Project"Key Innovation Team Construction Plan Cultivation Team(No.2018-CT-1)2018 Xiangyuan County Solid Waste Comprehensive Utilization Science and Technology Project(No.2018XYSDJS-05)the Shanxi Province Foundation for Selected(2019)Innovative Talents of Higher Education Institutions of Shanxi(2019)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2019L0031)Key R&D Program of Shanxi Province(No.201903D421069)Natural Science Foundation of Shanxi Province of China(No.201901D111015)Shanxi Province Foundation for Returness(No.2017-026)Program for the Innovative Talents of Higher Education Institutions of Shanxi(2019)Shanxi Collaborative Innovation Center of High Value-added Utilization of Coalrelated Wastes(No.2015-10-B3)Scientific Instrument Center of Shanxi University(No.201512)。
文摘The development of fluorescent probes enabling to distinguish Cys,Hcy and GSH has always been a considerable challenge,in particular the distinction of Hcy and other two biothiols,because Hcy has a very similar structure with Cys and a relatively lower concentration in living organisms.In this work,a special o-dialdehyde fluorescent probe,quinoline-2,3-dicarboxaldehyde(QDA),has been synthesized and demonstrated superior performance in differentiating detection of Hcy and GSH,which is different from the previous reported o-dialdehyde probes specifically detecting GSH.Furthermore,the probe can selectively distinguish Hcy and GSH from different signal channels in living cells and zebrafish,meaning it has great potential in biological applications.This finding will provide a novel idea for the design of fluorescent probes to distinguish biothiols.
