Template identification technology （TIT） is designed for the scenarios where a batch of disarmed nuclear weapons or components would be dismantled to observe a nuclear disarmament treaty. The core function played by...Template identification technology （TIT） is designed for the scenarios where a batch of disarmed nuclear weapons or components would be dismantled to observe a nuclear disarmament treaty. The core function played by the TIT is to make a judgment on whether the verified item belongs to a certain kind of nuclear weapons or component （NW/NC） or to which kind the verified item belongs. This paper analyses the functions played by the TIT in the process of NW/NC dismantlement, and proposes that two phases would be followed when applying the TIT： firstly to establish NW/NC templates with a sample of size n drawn from a certain kind of disarmament NW; secondly to authenticate NW/NC by means of the TIT. This paper also expatiates some terms related to the concept of the TIT and investigates on the development status of NW/NC TIT based on radiation signatures. The study concludes that the design of template structure is crucial to the establishment of an effective TIT and that starting from different research angles and aiming at the same goal of classification different template structures and corresponding template identification methods can be built up to meet specific identification requirements.展开更多
The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
文摘Template identification technology （TIT） is designed for the scenarios where a batch of disarmed nuclear weapons or components would be dismantled to observe a nuclear disarmament treaty. The core function played by the TIT is to make a judgment on whether the verified item belongs to a certain kind of nuclear weapons or component （NW/NC） or to which kind the verified item belongs. This paper analyses the functions played by the TIT in the process of NW/NC dismantlement, and proposes that two phases would be followed when applying the TIT： firstly to establish NW/NC templates with a sample of size n drawn from a certain kind of disarmament NW; secondly to authenticate NW/NC by means of the TIT. This paper also expatiates some terms related to the concept of the TIT and investigates on the development status of NW/NC TIT based on radiation signatures. The study concludes that the design of template structure is crucial to the establishment of an effective TIT and that starting from different research angles and aiming at the same goal of classification different template structures and corresponding template identification methods can be built up to meet specific identification requirements.
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
