Male reproductive development is necessary for the alternation of the life cycle in angiosperms.Due to functional redundancy of genes in the allohexaploid genome of common wheat,there are only two loci of recessive nu...Male reproductive development is necessary for the alternation of the life cycle in angiosperms.Due to functional redundancy of genes in the allohexaploid genome of common wheat,there are only two loci of recessive nuclear genic male sterility(GMS)mutations reported in wheat.Here,we report a new wheat recessive GMS gene,Ta Ms6,which encodes a GDSL esterase/lipase protein(GELP).Ta Ms6 is predominantly expressed in the anther during meiosis and the unicellular microspore stage,especially in meiotic cells(MCs),dyad cells,tapetum,and middle layer.The loss of Ta Ms6 function leads to male sterility,likely due to the downregulation of some pollen development-related genes and changes in lipid composition during meiosis.The ms6 mutant and Ms6 gene can potentially be utilized for developing commercialscale hybrid wheat breeding systems.We also systematically analyzed the GELP gene family in wheat,providing a comprehensive understanding of the Ta GELP family and offering valuable references for indepth genetic studies.Additionally,we discovered the nonallelic noncomplementation of two malesterile mutants,which presents an interesting and promising research direction.展开更多
基金supported by Shandong Provincial Natural Science Foundation(ZR2022ZD22,ZR2021ZD30,SYS202206)Shandong Agricultural University Dezhou(Qihe)Wheat Research Institute。
文摘Male reproductive development is necessary for the alternation of the life cycle in angiosperms.Due to functional redundancy of genes in the allohexaploid genome of common wheat,there are only two loci of recessive nuclear genic male sterility(GMS)mutations reported in wheat.Here,we report a new wheat recessive GMS gene,Ta Ms6,which encodes a GDSL esterase/lipase protein(GELP).Ta Ms6 is predominantly expressed in the anther during meiosis and the unicellular microspore stage,especially in meiotic cells(MCs),dyad cells,tapetum,and middle layer.The loss of Ta Ms6 function leads to male sterility,likely due to the downregulation of some pollen development-related genes and changes in lipid composition during meiosis.The ms6 mutant and Ms6 gene can potentially be utilized for developing commercialscale hybrid wheat breeding systems.We also systematically analyzed the GELP gene family in wheat,providing a comprehensive understanding of the Ta GELP family and offering valuable references for indepth genetic studies.Additionally,we discovered the nonallelic noncomplementation of two malesterile mutants,which presents an interesting and promising research direction.
