Artists:TANG Maohong Date:05.03,2019-07.14,2019 Duration:4 May-30 June,2019 Address:ShanghART Singapore,9 Lock Road,#02-22,Gillman Barracks,Singapore 108937Asolo exhibition by our artistin-residence,Tang Maohong,was s...Artists:TANG Maohong Date:05.03,2019-07.14,2019 Duration:4 May-30 June,2019 Address:ShanghART Singapore,9 Lock Road,#02-22,Gillman Barracks,Singapore 108937Asolo exhibition by our artistin-residence,Tang Maohong,was shown from 4 May to 14 July 2019.Taking inspiration from Paul Gauguin’s famed but controversial residency in the Tahiti,the exhibition title borrows from Gauguin’s travelogue Noa Noa:The Tahitian Journal.Such a reference reflects the artist’s initial impression of Southeast Asia as a place of lush greenery and azure skies.展开更多
Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2(ACE2), reported as a SARS-CoV-2 receptor, is also expressed in human mal...Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2(ACE2), reported as a SARS-CoV-2 receptor, is also expressed in human male testes. This suggests a potential risk in human male reproductive system. However, the characteristics of ACE2-positive cells and the expression of other SARS-CoV-2 process-related genes are still worthy of further investigation. Here, we performed singlecell RNA seq(scRNA-seq) analysis on 853 male embryo primordial germ cells(PGCs) and 2,854 normal testis cells to assess the effects of the SARS-CoV-2 virus on the male reproductive system from embryonic stage to adulthood. We also collected and constructed the scRNA-seq library on 228 Sertoli cells from three non-obstructive azoospermia(NOA) patients to assess the effects at disease state. We found that ACE2 expressing cells existed in almost all testis cell types and Sertoli cells had highest expression level and positive cells ratio. Moreover, ACE2 was also expressed in human male PGCs. In adulthood, the level of ACE2 expression decreased with the increase of age. We also found that ACE2 positive cells had high expressions of stress response and immune activation-related genes. Interestingly, some potential SARS-CoV-2 process-related genes such as TMPRSS2, BSG, CTSL and CTSB had different expression patterns in the same cell type. Furthermore, ACE2 expression level in NOA donors’ Sertoli cells was significantly decreased. Our work would help to assess the risk of SARS-CoV-2 infection in the male reproductive system.展开更多
Nitric oxide-associated protein 1 （NOA1） is involved in various abiotic stress responses and is required for plant resistance to pathogen infections. However, the role of NOA1 in plant-herbivore interactions has not...Nitric oxide-associated protein 1 （NOA1） is involved in various abiotic stress responses and is required for plant resistance to pathogen infections. However, the role of NOA1 in plant-herbivore interactions has not been explored. We created NOA1-silenced Nicotiana attenuata plants （irNaNOA1）. Compared with wild-type （WT） plants, irNaNOA1 plants had highly decreased photosynthesis rates. We further examined various traits important for plant defense against its specialist herbivore Manduca sexta by treating WT and irNaNOA1 plants with mechanical wounding and M. sexta oral secretions （OS）. NOA1-silenced plants showed elevated levels of herbivory-induced jasmonic acid （JA）, but decreased JA-isoleucine conjugate （JA-Ile） levels. The decreased JA-Ile levels did not result from compromised JAR （jasmonic acid resistant） activity in irNOA1 plants. Moreover, nitrogen-rich defensive compounds, nicotine and trypsin proteinase inhibitors, did not differ between WT and irNaNOA1 plants. In contrast, concentrations of most carbon-based defensive compounds were lower in these plants than in WT plants, although the levels of chlorogenic acid were not changed. Therefore, silencing NOA1 alters the allocation of carbon resources within the phenylpropanoid pathway. These data suggest the involvement of NOA1 in N. attenuata’s defense against M. sexta attack, and highlight its role in photosynthesis, and biosynthesis of jasmonates and secondary metabolites.展开更多
文摘Artists:TANG Maohong Date:05.03,2019-07.14,2019 Duration:4 May-30 June,2019 Address:ShanghART Singapore,9 Lock Road,#02-22,Gillman Barracks,Singapore 108937Asolo exhibition by our artistin-residence,Tang Maohong,was shown from 4 May to 14 July 2019.Taking inspiration from Paul Gauguin’s famed but controversial residency in the Tahiti,the exhibition title borrows from Gauguin’s travelogue Noa Noa:The Tahitian Journal.Such a reference reflects the artist’s initial impression of Southeast Asia as a place of lush greenery and azure skies.
基金funded by Beijing Municipal Science & Technology Commission (Z191100006619075)the National Natural Science Foundation of China (81521002 and 81730038)。
文摘Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2(ACE2), reported as a SARS-CoV-2 receptor, is also expressed in human male testes. This suggests a potential risk in human male reproductive system. However, the characteristics of ACE2-positive cells and the expression of other SARS-CoV-2 process-related genes are still worthy of further investigation. Here, we performed singlecell RNA seq(scRNA-seq) analysis on 853 male embryo primordial germ cells(PGCs) and 2,854 normal testis cells to assess the effects of the SARS-CoV-2 virus on the male reproductive system from embryonic stage to adulthood. We also collected and constructed the scRNA-seq library on 228 Sertoli cells from three non-obstructive azoospermia(NOA) patients to assess the effects at disease state. We found that ACE2 expressing cells existed in almost all testis cell types and Sertoli cells had highest expression level and positive cells ratio. Moreover, ACE2 was also expressed in human male PGCs. In adulthood, the level of ACE2 expression decreased with the increase of age. We also found that ACE2 positive cells had high expressions of stress response and immune activation-related genes. Interestingly, some potential SARS-CoV-2 process-related genes such as TMPRSS2, BSG, CTSL and CTSB had different expression patterns in the same cell type. Furthermore, ACE2 expression level in NOA donors’ Sertoli cells was significantly decreased. Our work would help to assess the risk of SARS-CoV-2 infection in the male reproductive system.
文摘Nitric oxide-associated protein 1 （NOA1） is involved in various abiotic stress responses and is required for plant resistance to pathogen infections. However, the role of NOA1 in plant-herbivore interactions has not been explored. We created NOA1-silenced Nicotiana attenuata plants （irNaNOA1）. Compared with wild-type （WT） plants, irNaNOA1 plants had highly decreased photosynthesis rates. We further examined various traits important for plant defense against its specialist herbivore Manduca sexta by treating WT and irNaNOA1 plants with mechanical wounding and M. sexta oral secretions （OS）. NOA1-silenced plants showed elevated levels of herbivory-induced jasmonic acid （JA）, but decreased JA-isoleucine conjugate （JA-Ile） levels. The decreased JA-Ile levels did not result from compromised JAR （jasmonic acid resistant） activity in irNOA1 plants. Moreover, nitrogen-rich defensive compounds, nicotine and trypsin proteinase inhibitors, did not differ between WT and irNaNOA1 plants. In contrast, concentrations of most carbon-based defensive compounds were lower in these plants than in WT plants, although the levels of chlorogenic acid were not changed. Therefore, silencing NOA1 alters the allocation of carbon resources within the phenylpropanoid pathway. These data suggest the involvement of NOA1 in N. attenuata’s defense against M. sexta attack, and highlight its role in photosynthesis, and biosynthesis of jasmonates and secondary metabolites.
