Up to date,application of nanomaterials and nanotechnology has made great advances.Many novel nanomaterials with unique properties are increasingly being exploited to apply for biosensors,improving the property of bio...Up to date,application of nanomaterials and nanotechnology has made great advances.Many novel nanomaterials with unique properties are increasingly being exploited to apply for biosensors,improving the property of biosensor and making them higher selectivity and sensitivity,less response time and lower detective limitation.Here we review some of the main advances in this field over the past few years,explore the application prospects,and discuss the issues,approaches,and challenges,with the amin of promting to develop nanomaterials-based biosensor nanotechnology and improving their application in disease diagnosis and biosafety examination.展开更多
Herein we reported the effects of generation 5 polyamidoamine dendrimer-functionalized fluorescent multi-walled carbon nanotubes(dMNTs)on mice embryonic stem cell line CCE.The dMNTs were prepared and characterized,and...Herein we reported the effects of generation 5 polyamidoamine dendrimer-functionalized fluorescent multi-walled carbon nanotubes(dMNTs)on mice embryonic stem cell line CCE.The dMNTs were prepared and characterized,and incubated with murine embryonic stem cell CCE cells(ES)for 1 to 5 day.These ES cells were observed by fluorescent microscopy,and were analyzed by flow cytometer and MTT.Results showed that the dendrimer--1 functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized,could enter into ES cells quickly,more than 20µg mL dose caused ES cells became smaller and smaller as the incubation time increased,and inhibited cell growth in dose-and time-dependent means,-1 less than 5µg mL dose improves ES differentiation.These results demonstrate that dMNTs are toxic to ES cells at large dose,can induce ES cells’differentiation at lower dose.The prepared dMNTs may be a good dual-phase regulation reagent to exposed ES cells,and have potential applications in research and development of ES cells.展开更多
This paper describes a simple strategy for covalently attaching silica-coated gold nanorods(sGNRs)onto the surface of multi-walled carbon nanotubes(MWNT)to fabricate hybrid nanostructures.The crosslinked reaction occu...This paper describes a simple strategy for covalently attaching silica-coated gold nanorods(sGNRs)onto the surface of multi-walled carbon nanotubes(MWNT)to fabricate hybrid nanostructures.The crosslinked reaction occurs through interaction of carboxyl groups on the MWNT with the amino silane coupling agent modified sGNRs.TEM,FT-IR spectroscopy,UV-vis spectroscopy,and Zeta potential analysis have been used to study the formation of MWNT/sGNRs nanostructure.Furthermore,MWNT/sGNRs hybrid material shows an excellent solubility in aqueous solution and an enhanced absorption in the near-infrared region.It should open up new possibilities in nanomedicine as multimodal photoacoustic and photothermal high-contrat molecular agent.展开更多
文摘Up to date,application of nanomaterials and nanotechnology has made great advances.Many novel nanomaterials with unique properties are increasingly being exploited to apply for biosensors,improving the property of biosensor and making them higher selectivity and sensitivity,less response time and lower detective limitation.Here we review some of the main advances in this field over the past few years,explore the application prospects,and discuss the issues,approaches,and challenges,with the amin of promting to develop nanomaterials-based biosensor nanotechnology and improving their application in disease diagnosis and biosafety examination.
基金supported by the budget from Strategic Fund for Establishing the International HQs of Universities in Waseda Universitythe National 973 project(2010CB933901)+2 种基金863 Project(No.2007AA022004)Shanghai Pujian Plan Project(No.06PJ14049)Shanghai Foundation of Science and Technology(No.054119527 and 0752nm024).
文摘Herein we reported the effects of generation 5 polyamidoamine dendrimer-functionalized fluorescent multi-walled carbon nanotubes(dMNTs)on mice embryonic stem cell line CCE.The dMNTs were prepared and characterized,and incubated with murine embryonic stem cell CCE cells(ES)for 1 to 5 day.These ES cells were observed by fluorescent microscopy,and were analyzed by flow cytometer and MTT.Results showed that the dendrimer--1 functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized,could enter into ES cells quickly,more than 20µg mL dose caused ES cells became smaller and smaller as the incubation time increased,and inhibited cell growth in dose-and time-dependent means,-1 less than 5µg mL dose improves ES differentiation.These results demonstrate that dMNTs are toxic to ES cells at large dose,can induce ES cells’differentiation at lower dose.The prepared dMNTs may be a good dual-phase regulation reagent to exposed ES cells,and have potential applications in research and development of ES cells.
基金supported by the National Key Basic Research Program(973 Project)(2010CB933901)National 863 Hi-tech Project(2007AA022004)+6 种基金Important National Science&Technology Specific Projects(2009ZX10004-311)National Natural Scientific Fund(No.20771075 and 20803040)Special project for nano-technology from Shanghai(No.1052nm04100)New Century Excellent Talent of Ministry of Education of China(NCET-08-0350)Shanghai Science and Technology Fund(10XD1406100)Doctoral Program of Higher Education Research Fund(20070248050,20070248107)Shanghai Jiao Tong University Innovation Fund For Postgraduates.
文摘This paper describes a simple strategy for covalently attaching silica-coated gold nanorods(sGNRs)onto the surface of multi-walled carbon nanotubes(MWNT)to fabricate hybrid nanostructures.The crosslinked reaction occurs through interaction of carboxyl groups on the MWNT with the amino silane coupling agent modified sGNRs.TEM,FT-IR spectroscopy,UV-vis spectroscopy,and Zeta potential analysis have been used to study the formation of MWNT/sGNRs nanostructure.Furthermore,MWNT/sGNRs hybrid material shows an excellent solubility in aqueous solution and an enhanced absorption in the near-infrared region.It should open up new possibilities in nanomedicine as multimodal photoacoustic and photothermal high-contrat molecular agent.
