目的观察安神补脑液(ASBN)对失眠大鼠海马体γ-氨基丁酸受体(GABA)、钠-钾-氯协同转运蛋白表达的影响及其催眠作用机制。方法采用腹腔注射对氯苯丙氨酸(PCPA)复制大鼠失眠模型,将复制成功的失眠模型大鼠随机分为氟西汀(Fluoxetine)组,...目的观察安神补脑液(ASBN)对失眠大鼠海马体γ-氨基丁酸受体(GABA)、钠-钾-氯协同转运蛋白表达的影响及其催眠作用机制。方法采用腹腔注射对氯苯丙氨酸(PCPA)复制大鼠失眠模型,将复制成功的失眠模型大鼠随机分为氟西汀(Fluoxetine)组,模型组,ASBN高、中、低剂量(ASBN-H、M、L)组,另取15只正常大鼠作为正常组。各给药组连续7 d给予相应药物口服灌胃,模型组和正常组连续灌胃7 d 0.9%生理盐水,观察各组大鼠的一般活动状态。采用qPCR检测海马体中GABA_ARγ2和Na+-K+-Cl-协同转运蛋白(NKCC1)的表达,采用免疫组化染色(IHC)-P检测大鼠海马体中GABA_ARα1的表达。结果ASBN可提高海马体中GABA_ARα1、GABA_ARγ2表达,降低NKCC1表达。结论ASBN可以通过调节失眠大鼠海马体中GABA_ARα1、GABA_ARγ2和NKCC1表达量来改善大鼠的睡眠状态。展开更多
The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b ...The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.展开更多
文摘目的观察安神补脑液(ASBN)对失眠大鼠海马体γ-氨基丁酸受体(GABA)、钠-钾-氯协同转运蛋白表达的影响及其催眠作用机制。方法采用腹腔注射对氯苯丙氨酸(PCPA)复制大鼠失眠模型,将复制成功的失眠模型大鼠随机分为氟西汀(Fluoxetine)组,模型组,ASBN高、中、低剂量(ASBN-H、M、L)组,另取15只正常大鼠作为正常组。各给药组连续7 d给予相应药物口服灌胃,模型组和正常组连续灌胃7 d 0.9%生理盐水,观察各组大鼠的一般活动状态。采用qPCR检测海马体中GABA_ARγ2和Na+-K+-Cl-协同转运蛋白(NKCC1)的表达,采用免疫组化染色(IHC)-P检测大鼠海马体中GABA_ARα1的表达。结果ASBN可提高海马体中GABA_ARα1、GABA_ARγ2表达,降低NKCC1表达。结论ASBN可以通过调节失眠大鼠海马体中GABA_ARα1、GABA_ARγ2和NKCC1表达量来改善大鼠的睡眠状态。
基金supported by the China Agriculture Research System (No. CARS-47)the National Natural Science Foundation of China (No. 31602147)
文摘The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.
