Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive f...Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.展开更多
Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertensi...Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertension,jaundice,dysmenorrheal,gastrointestinal disorders and malaria.In order to reveal its full pharmacological and therapeutic potentials,die present review focuses on the current medicinal uses,phytochemistry,pharmacological and toxicological activities of this species.Literature survey on scientific journals,books as well as electronic sources have shown the isolation of alkaloids,tannins,polyphenols and steroids from different parts of the plant,pharmacological studies revealed that the extract or isolated compounds from this species posses analgesic,anti-inflammatory,hypoglyceamic,hypotensive,antiplasmodial,antimicrobial,antiulcer and antitumorigenic activities.Results from various scientific investigations to date have revealed the potential of the extract from the plant or isolated compounds for use in the treatment and prevention of various kinds of human diseases.However,further studies on the extracts and pure compounds from this species is required to completely assess its phytoc-hemical,pharmacological and toxicological profile as well as the mechanism of action behind these pharmacological activities exhibited by the compounds isolated from this species.展开更多
Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing b...Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.展开更多
Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous f...Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous fraction of Strychnos nitida.Their structures were elucidated on the basis of extensive analysis of spectroscopic data.All the alkaloids were tested for their cytotoxic activity,but they did not show any exciting result.展开更多
Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionat...Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionated into chloroform fraction and methanol fraction.They were evaluated for antiulcer activity and gastric emptying time in rats using aspirin-pylorus-ligation model.Results:Oral administration of the methanol extract,chloroform fraction and methanol fraction at 1 000 mg/kg reduced gastric ulcer by 56.4%,40.0%and 56.3%,respectively;and the fractions of the extract significantly(P【0.05) reduced gastric emptying time when compared to the control.Gastric acidity was significandy decreased when compared with saline group,40.25 mEq/ L in methanol extract,50.0 mEq/L in chloroform fraction 51.25 mEq/L in methanol fration but had no significant effect on the gastric secretion volume.Conclusions:These findings showed that methanol extract,chloroform fraction and methanol fraction of the seeds of Picralima possessed potent antiulcer properties and some antisecretory properties.展开更多
The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on o...The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on organoleptic and physical properties of cola nut during storage. A biopesticide, glucose syrup and biopesticide + glucose syrup were applied to fresh cola nuts before conditioning and kept at 28°C for 6 weeks. Physicochemical and sensory analyses were performed to check the quality of the nuts during storage. The results showed that the biopesticide keep cola nuts better than the others methods with only 11.66% ± 3.04% and 13.66% ± 3.95% of loss rates for white and red cola nuts respectively. Cola nuts treated with bio-pesticide retain significantly their freshness with 62.00% ± 1.15% of moisture for white nuts and 64.00% ± 2.00% of moisture for red nuts compared to those treated with glucose syrup and bio-pesticide + glucose syrup (56.66% ± 1.15%). Cola nuts treated with biopesticide have a better acceptability compared to those subjected to others treatments. The use of biopesticide for the storage of cola nuts minimizes the losses and maintains the quality whatever the type of cola.展开更多
The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antio...The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antioxidant activity of crude extracts of both plants was investigated using the scavenging effect on DPPH(2,2-Diphenyl-1-Picrylhydrazyl Radical)assay,while the total phenolic content was determined by the Folin-Ciocalteu reagent method.When compared to standard drug vitamin C at various concentrations,both extracts exhibited potent antioxidant activity that increased with increasing concentration of extracts.The high positive linear correlation between the antioxidant capacity and the total phenolic content suggests that the antioxidant capacity of both plants can be largely attributed to their phenolic compounds.Based on these findings,O.aubrevillei fruit and P.nitida leaf could be potential natural antioxidant sources for preventing free radical-induced diseases.展开更多
Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine ...Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine the bamboo ramet population growth of Fargesla nitida （Mltford） Keng f. et Yi and to determine how ramet population structure varies along the succession of coniferous forest, we sampled ramet populations of F. nitida from the following three successional stages： （i） a deciduous broad-leaved （BL） stand; （ii） a mixed broad-leaved coniferous （MI） stand; and （ill） a coniferous （CF） stand. We investigated the population structure, biomass allocation, and morphological characteristics of the bamboo ramet among the three stand types. Clonal ramets, constituting the bamboo population, tended to become short and small with succession. The ramet changed towards having a greater mass investment in leaves, branches and underground roots and rhizomes rather than in the culm. With respect to leaf traits, individual leaf mass and area in the BL stand were markedly bigger than those In both the MI and CF stands, except for no significant difference in specific leaf area. The age distribution showed that the bamboo population approached an older age with succession. The results demonstrate that the ramet population structure of F. nitida is unstable and its growth performance is inhibited by succession.展开更多
The aim of this study is to explore the effects of canopy conditions on clump and culm numbers,and the morphological plasticity and biomass distribution patterns of the dwarf bamboo species Fargesia nitida.Specificall...The aim of this study is to explore the effects of canopy conditions on clump and culm numbers,and the morphological plasticity and biomass distribution patterns of the dwarf bamboo species Fargesia nitida.Specifically,we investigated the effects of canopy conditions on the growth and morphological characteristics of F.nitida,and the adaptive responses of F.nitida to different canopy conditions and its ecological senses.The results indicate that forest canopy had a significant effect on the genet density and culm number per clump,while it did not affect the ramet density.Clumps tended to be few and large in gaps and forest edge plots,and small under forest understory plots.The ramets showed an even distribution under the closed canopy,and cluster distribution under gaps and forest edge plots.The forest canopy had a significant effect on both the ramets’biomass and biomass allocation.Favourable light conditions promoted ramet growth and biomass accumulation.Greater amounts of biomass in gaps and forest edge plots were shown by the higher number of culms per clump and the diameter of these culms.Under closed canopy,the bamboos increased their branching angle,leaf biomass allocation,specific leaf area and leaf area ratio to exploit more favourable light conditions in these locations.The spacer length,specific spacer length and spacer branching angles all showed significant differences between gaps and closed canopy conditions.The larger specific spacer length and spacer branching angle were beneficial for bamboo growth,scattering the ramets and exploiting more favourable light conditions.In summary,this study shows that to varying degrees,F.nitida exhibits both a wide ecological amplitude and high degree of morphological plasticity in response to differing forest canopy conditions.Moreover,the changes in plasticity enable the plants to optimize their light usage efficiency to promote growth and increase access to resources available in heterogeneous light environments.展开更多
基金supported by the 985 Project of Peking University Health Science Center (No. 985-2-119-121)
文摘Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.
基金supported by a US-Senior Fulbright Award granted to Dr.A.Falodun for study at University of Mississippi,USA,CIESCs for the Fulbright awardthe University of Benin(Nigeria)and Institute of Chemistry(University of Rostock,Germany),for their support
文摘Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertension,jaundice,dysmenorrheal,gastrointestinal disorders and malaria.In order to reveal its full pharmacological and therapeutic potentials,die present review focuses on the current medicinal uses,phytochemistry,pharmacological and toxicological activities of this species.Literature survey on scientific journals,books as well as electronic sources have shown the isolation of alkaloids,tannins,polyphenols and steroids from different parts of the plant,pharmacological studies revealed that the extract or isolated compounds from this species posses analgesic,anti-inflammatory,hypoglyceamic,hypotensive,antiplasmodial,antimicrobial,antiulcer and antitumorigenic activities.Results from various scientific investigations to date have revealed the potential of the extract from the plant or isolated compounds for use in the treatment and prevention of various kinds of human diseases.However,further studies on the extracts and pure compounds from this species is required to completely assess its phytoc-hemical,pharmacological and toxicological profile as well as the mechanism of action behind these pharmacological activities exhibited by the compounds isolated from this species.
基金funded by the “14th Five-Year Plan” team-building projects of Guangdong Academy Agricultural Sciences (202126TD)National Natural Science Foundation of China (81903319, 81803236, 31800295)+10 种基金Guangdong Science and Technology program (2018KJYZ002)Guangdong Basic and Applied Basic Research Foundation (2020A1515011266)Qingyuan Science and Technology Program (DZXQY021, 181022114566189, 181022114566189, 2019A039, 2020KJJH042)Shaoguan Science and Technology Program (2018CS11902, 2018sn081)Guangzhou Science and Technology Program (202002030202)Zhaoqing Science and Technology Program (2019N001, 2019N013)Maoming Science and Technology Program (mmkj2020045)Zhanjiang Science and Technology Program (2020A03014)Yingde Science and Technology Board (JHXM2018029)Innovation Fund projects of Guangdong Academy of Agricultural Sciences (202115)Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (R2019PY-JX004, R2018YJ-YB3002, R2016YJYB3003, R2018PY-QF005, R2018QD-101)。
文摘Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.
基金the National Natural Science Foundation of China(81225024).
文摘Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous fraction of Strychnos nitida.Their structures were elucidated on the basis of extensive analysis of spectroscopic data.All the alkaloids were tested for their cytotoxic activity,but they did not show any exciting result.
文摘Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionated into chloroform fraction and methanol fraction.They were evaluated for antiulcer activity and gastric emptying time in rats using aspirin-pylorus-ligation model.Results:Oral administration of the methanol extract,chloroform fraction and methanol fraction at 1 000 mg/kg reduced gastric ulcer by 56.4%,40.0%and 56.3%,respectively;and the fractions of the extract significantly(P【0.05) reduced gastric emptying time when compared to the control.Gastric acidity was significandy decreased when compared with saline group,40.25 mEq/ L in methanol extract,50.0 mEq/L in chloroform fraction 51.25 mEq/L in methanol fration but had no significant effect on the gastric secretion volume.Conclusions:These findings showed that methanol extract,chloroform fraction and methanol fraction of the seeds of Picralima possessed potent antiulcer properties and some antisecretory properties.
文摘The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on organoleptic and physical properties of cola nut during storage. A biopesticide, glucose syrup and biopesticide + glucose syrup were applied to fresh cola nuts before conditioning and kept at 28°C for 6 weeks. Physicochemical and sensory analyses were performed to check the quality of the nuts during storage. The results showed that the biopesticide keep cola nuts better than the others methods with only 11.66% ± 3.04% and 13.66% ± 3.95% of loss rates for white and red cola nuts respectively. Cola nuts treated with bio-pesticide retain significantly their freshness with 62.00% ± 1.15% of moisture for white nuts and 64.00% ± 2.00% of moisture for red nuts compared to those treated with glucose syrup and bio-pesticide + glucose syrup (56.66% ± 1.15%). Cola nuts treated with biopesticide have a better acceptability compared to those subjected to others treatments. The use of biopesticide for the storage of cola nuts minimizes the losses and maintains the quality whatever the type of cola.
文摘The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antioxidant activity of crude extracts of both plants was investigated using the scavenging effect on DPPH(2,2-Diphenyl-1-Picrylhydrazyl Radical)assay,while the total phenolic content was determined by the Folin-Ciocalteu reagent method.When compared to standard drug vitamin C at various concentrations,both extracts exhibited potent antioxidant activity that increased with increasing concentration of extracts.The high positive linear correlation between the antioxidant capacity and the total phenolic content suggests that the antioxidant capacity of both plants can be largely attributed to their phenolic compounds.Based on these findings,O.aubrevillei fruit and P.nitida leaf could be potential natural antioxidant sources for preventing free radical-induced diseases.
基金Supported by the State Key Basic Research and Development Plan of China （2002CB111505） and the National Nature Science Foundation of China （30300047, 30500388）. Acknowledgements The authors thank Hans Cornelissen （Vrije Universiteit, Amsterdam, The Netherlands） and Ken Chart （University of the Sunshine Coast, 0ueensland, Australia） for their valuable comments on our earlier manuscript. Thanks are also due to the students from Nanjing Forestry University and Sichuan Agricultural University and the staff of Wolong Subalpine Dark Coniferous Forest Ecosystem Ecological Station, Sichuan Academy of Forestry, who helped with the field work.
文摘Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine the bamboo ramet population growth of Fargesla nitida （Mltford） Keng f. et Yi and to determine how ramet population structure varies along the succession of coniferous forest, we sampled ramet populations of F. nitida from the following three successional stages： （i） a deciduous broad-leaved （BL） stand; （ii） a mixed broad-leaved coniferous （MI） stand; and （ill） a coniferous （CF） stand. We investigated the population structure, biomass allocation, and morphological characteristics of the bamboo ramet among the three stand types. Clonal ramets, constituting the bamboo population, tended to become short and small with succession. The ramet changed towards having a greater mass investment in leaves, branches and underground roots and rhizomes rather than in the culm. With respect to leaf traits, individual leaf mass and area in the BL stand were markedly bigger than those In both the MI and CF stands, except for no significant difference in specific leaf area. The age distribution showed that the bamboo population approached an older age with succession. The results demonstrate that the ramet population structure of F. nitida is unstable and its growth performance is inhibited by succession.
基金The study was supported by the National Basic Research Program of China(No.2002CB111505)The National Natural Science Foundation of China(Grant Nos.30500388,30300047).
文摘The aim of this study is to explore the effects of canopy conditions on clump and culm numbers,and the morphological plasticity and biomass distribution patterns of the dwarf bamboo species Fargesia nitida.Specifically,we investigated the effects of canopy conditions on the growth and morphological characteristics of F.nitida,and the adaptive responses of F.nitida to different canopy conditions and its ecological senses.The results indicate that forest canopy had a significant effect on the genet density and culm number per clump,while it did not affect the ramet density.Clumps tended to be few and large in gaps and forest edge plots,and small under forest understory plots.The ramets showed an even distribution under the closed canopy,and cluster distribution under gaps and forest edge plots.The forest canopy had a significant effect on both the ramets’biomass and biomass allocation.Favourable light conditions promoted ramet growth and biomass accumulation.Greater amounts of biomass in gaps and forest edge plots were shown by the higher number of culms per clump and the diameter of these culms.Under closed canopy,the bamboos increased their branching angle,leaf biomass allocation,specific leaf area and leaf area ratio to exploit more favourable light conditions in these locations.The spacer length,specific spacer length and spacer branching angles all showed significant differences between gaps and closed canopy conditions.The larger specific spacer length and spacer branching angle were beneficial for bamboo growth,scattering the ramets and exploiting more favourable light conditions.In summary,this study shows that to varying degrees,F.nitida exhibits both a wide ecological amplitude and high degree of morphological plasticity in response to differing forest canopy conditions.Moreover,the changes in plasticity enable the plants to optimize their light usage efficiency to promote growth and increase access to resources available in heterogeneous light environments.
