Objective:Polygoni Multiflori Radix(PM)is a traditional herbal medicine with repeated reports of liver injury events in recent years.We wondered whether the classical processing method,namely,nine-time steaming and su...Objective:Polygoni Multiflori Radix(PM)is a traditional herbal medicine with repeated reports of liver injury events in recent years.We wondered whether the classical processing method,namely,nine-time steaming and sun drying(NSSD),had toxicity-attenuating effects on PM and the relationships between toxicity and times of processing,as well as with the alteration trends of its compounds.Materials and Methods:The chemical fingerprints of different PM extracts were developed using ultra-high-performance liquid chromatography.The spectrum-toxicity correlation between the chemical fingerprints and hepatocellular toxicity was analyzed with multiple correlation analysis.Results:The results suggested that the hepatotoxicity of NSSD processing products markedly decreased with the repeated steaming and sun drying,which was obviously superior to the product processed by the modern method.Comprehensive analysis revealed that the contents of cis-stilbene glycoside and emodin-8-O-β-D-glucoside related to liver injury susceptibility were reduced with the times of NSSD processing,which was consistent with the decreased trend of hepatocellular toxicity.After the five times of NSSD,the contents of them as well as the hepatotoxicity of PM were steady.Moreover,we found that the contents of catechin and physcion declined rapidly after the one time of NSSD and then remained stable until the nine times of NSSD.Based on the fact,they could be utilized to indicate whether PM products were processed by steaming and sun drying.Conclusions:This paper confirmed that the NSSD had a good influence on the toxicity attenuating to PM and found four compounds which could apply for the quality control of PM.展开更多
目的基于物质基础的差异及其与炮制工艺的相关性探讨大黄Rhei Radix et Rhizoma古(九蒸九晒)、今(药典法)炮制方法的合理性。方法采用HPLC法对古、今方法炮制的大黄饮片整体化学轮廓特征进行表征,并对大黄酚-8-O-β-D-葡萄糖苷、大黄酚...目的基于物质基础的差异及其与炮制工艺的相关性探讨大黄Rhei Radix et Rhizoma古(九蒸九晒)、今(药典法)炮制方法的合理性。方法采用HPLC法对古、今方法炮制的大黄饮片整体化学轮廓特征进行表征,并对大黄酚-8-O-β-D-葡萄糖苷、大黄酚、没食子酸等15个主要药效成分进行含量测定,结合化学计量学对古、今方法炮制的大黄饮片中苷类、苷元类、鞣质类成分的含量及量比关系进行分析,对大黄不同饮片中主要成分进行正交偏最小二乘法-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)与系统聚类分析(hierarchical cluster analysis,HCA),以变量重要性投影(variable importance projection,VIP)值>1,筛选出具有统计学意义的差异标志物。结果大黄经古、今2种方法炮制而成的饮片,化学组成较为相似,均含有大黄酚-8-O-β-D-葡萄糖苷、大黄酚、没食子酸等14种化学成分,但上述成分的含量和比例具有显著差异。OPLS-DA结果显示,古、今2种方法炮制的大黄饮片分布在不同的象限,HCA结果显示,大黄不同饮片可各自聚类。以VIP值>1共筛选得到没食子酸、白藜芦醇-4′-O-β-D-(6″-没食子酰基)-葡萄糖苷、大黄酸-8-O-β-D-葡萄糖苷、儿茶素、芦荟大黄素-8-O-β-D-葡萄糖苷、大黄素,可以作为区分古、今方法炮制饮片的标志物。结论通过对古、今2种方法炮制后大黄化学成分组成及其量比关系差异的分析,揭示了炮制方法对大黄饮片质量属性内涵形成的影响,明确了九蒸九晒大黄与药典法熟大黄的物质基础,为进一步结合药效学研究全面了解二者的差异奠定了基础。展开更多
基金funded by the National Natural Science Foundation of China(Grant No.81630100)the National Industry Program of China(Grant No.201507002)
文摘Objective:Polygoni Multiflori Radix(PM)is a traditional herbal medicine with repeated reports of liver injury events in recent years.We wondered whether the classical processing method,namely,nine-time steaming and sun drying(NSSD),had toxicity-attenuating effects on PM and the relationships between toxicity and times of processing,as well as with the alteration trends of its compounds.Materials and Methods:The chemical fingerprints of different PM extracts were developed using ultra-high-performance liquid chromatography.The spectrum-toxicity correlation between the chemical fingerprints and hepatocellular toxicity was analyzed with multiple correlation analysis.Results:The results suggested that the hepatotoxicity of NSSD processing products markedly decreased with the repeated steaming and sun drying,which was obviously superior to the product processed by the modern method.Comprehensive analysis revealed that the contents of cis-stilbene glycoside and emodin-8-O-β-D-glucoside related to liver injury susceptibility were reduced with the times of NSSD processing,which was consistent with the decreased trend of hepatocellular toxicity.After the five times of NSSD,the contents of them as well as the hepatotoxicity of PM were steady.Moreover,we found that the contents of catechin and physcion declined rapidly after the one time of NSSD and then remained stable until the nine times of NSSD.Based on the fact,they could be utilized to indicate whether PM products were processed by steaming and sun drying.Conclusions:This paper confirmed that the NSSD had a good influence on the toxicity attenuating to PM and found four compounds which could apply for the quality control of PM.
