Keratinous wastes could be degraded by some microorganisms in nature. Native human foot skin （NHFS） was used as sole nitrogen source to screen microorganisms with keratin-degrading capability. From approximately 200...Keratinous wastes could be degraded by some microorganisms in nature. Native human foot skin （NHFS） was used as sole nitrogen source to screen microorganisms with keratin-degrading capability. From approximately 200 strains, a strain of Streptomyces sp. strain No. 16 was found to possess the strongest keratinolytic activity, and the total activity in the culture was 110 KU/ml with specific activity of 2870 KU/mg protein （KU： keratinase unit）. Substrate specificity test indicated that the crude keratinase could degrade keratin azure, human hair, cock feathers and collagen. The optimal pH of the crude keratinase ranged from 7.5 to 10 and the temperature ranged from 40℃ to 55℃. Metal chelating agent ethylenediamine tetraacetic acid obviously stimulated the keratinolytic activity but suppressed the proteolytic activity. To our knowledge, this is the first report on specific induction of keratinases by NHFS from an actinomycete. Moreover, excellent characteristics of its crude keratinase may lead to the potential application in waste treatment and recovery, poultry and leather industry, medicine, and cosmetic development.展开更多
Aim: The purpose of this case study was to examine the sleep quality of patients receiving noninvasive positive pressure ventilation (NPPV) or nasal high-flow oxygen therapy (NHF) in an intensive care unit and to inve...Aim: The purpose of this case study was to examine the sleep quality of patients receiving noninvasive positive pressure ventilation (NPPV) or nasal high-flow oxygen therapy (NHF) in an intensive care unit and to investigate what types of nursing support are offered to such patients. Methods: We examined one patient each for NPPV and NHF. Polysomnography (PSG), review of the patient charts, and semi-structured interviews were used to collect the data for analysis. Results: Patients treated with NPPV or NHF demonstrated a noticeable reduction in deep sleep, with most of their sleep being shallow. Their sleep patterns varied greatly from those of healthy individuals. These results suggest that, in addition to experiencing extremely fragmented sleep, sleep in these patients was more likely to be interrupted by nursing interventions, such as during auscultation of breath sounds. Furthermore, it was revealed that “anxiety or discomfort that accompanies the mask or air pressure” in patients treated with NPPV and “discomfort that accompanies the nasal cannula or NHF circuit” in patients treated with NHF may be primary causes of disrupted sleep. Our results suggest a need for nursing care aimed at improving sleep quality in patients treated with NPPV or NHF.展开更多
Nickel hydroxyfluoride(NHF)with an elevated theoretical specific capacity has attracted increasing interest for use as an electrode material but its deficient electrical conductivity and inadequate number of active si...Nickel hydroxyfluoride(NHF)with an elevated theoretical specific capacity has attracted increasing interest for use as an electrode material but its deficient electrical conductivity and inadequate number of active sites have hampered its wide applications.Herein,small amounts of Fe were doped into NHF by a novel single-step solvothermal method.The experimental findings revealed that doping of Fe into NHF accelerated the electron transfer at the Ni sites and promoted the generation of high valence Ni^((3+δ)+)for stimulating a two-step reaction.The presence of highly active Ni^((3+δ)+)enriched the redox process and effectively enhanced energy storage.At a current density of 1 A g^(−1),the optimal doped sample NHF-0.03 displayed a maximum specific capacity of 640 C g^(−1).The assembled NHF-0.03//AC asymmetric supercapacitor exhibited a maximum power density of 750 W kg^(−1)and a maximum energy density of 55.5 W h kg^(−1).After 10000 charge/discharge cycles,the supercapacitor device preserved 77%of its original capacity at a high current density of 15 A g^(−1),indicating good cycling stability.Overall,the proposed innovative doping strategy looks promising for the enhancement and refinement of materials based on hydroxyfluoride for advanced supercapacitor electrodes.展开更多
文摘Keratinous wastes could be degraded by some microorganisms in nature. Native human foot skin （NHFS） was used as sole nitrogen source to screen microorganisms with keratin-degrading capability. From approximately 200 strains, a strain of Streptomyces sp. strain No. 16 was found to possess the strongest keratinolytic activity, and the total activity in the culture was 110 KU/ml with specific activity of 2870 KU/mg protein （KU： keratinase unit）. Substrate specificity test indicated that the crude keratinase could degrade keratin azure, human hair, cock feathers and collagen. The optimal pH of the crude keratinase ranged from 7.5 to 10 and the temperature ranged from 40℃ to 55℃. Metal chelating agent ethylenediamine tetraacetic acid obviously stimulated the keratinolytic activity but suppressed the proteolytic activity. To our knowledge, this is the first report on specific induction of keratinases by NHFS from an actinomycete. Moreover, excellent characteristics of its crude keratinase may lead to the potential application in waste treatment and recovery, poultry and leather industry, medicine, and cosmetic development.
文摘Aim: The purpose of this case study was to examine the sleep quality of patients receiving noninvasive positive pressure ventilation (NPPV) or nasal high-flow oxygen therapy (NHF) in an intensive care unit and to investigate what types of nursing support are offered to such patients. Methods: We examined one patient each for NPPV and NHF. Polysomnography (PSG), review of the patient charts, and semi-structured interviews were used to collect the data for analysis. Results: Patients treated with NPPV or NHF demonstrated a noticeable reduction in deep sleep, with most of their sleep being shallow. Their sleep patterns varied greatly from those of healthy individuals. These results suggest that, in addition to experiencing extremely fragmented sleep, sleep in these patients was more likely to be interrupted by nursing interventions, such as during auscultation of breath sounds. Furthermore, it was revealed that “anxiety or discomfort that accompanies the mask or air pressure” in patients treated with NPPV and “discomfort that accompanies the nasal cannula or NHF circuit” in patients treated with NHF may be primary causes of disrupted sleep. Our results suggest a need for nursing care aimed at improving sleep quality in patients treated with NPPV or NHF.
基金supported by the Scientific and Technological Development Program of Jilin Province(grant no.20240101114JC).
文摘Nickel hydroxyfluoride(NHF)with an elevated theoretical specific capacity has attracted increasing interest for use as an electrode material but its deficient electrical conductivity and inadequate number of active sites have hampered its wide applications.Herein,small amounts of Fe were doped into NHF by a novel single-step solvothermal method.The experimental findings revealed that doping of Fe into NHF accelerated the electron transfer at the Ni sites and promoted the generation of high valence Ni^((3+δ)+)for stimulating a two-step reaction.The presence of highly active Ni^((3+δ)+)enriched the redox process and effectively enhanced energy storage.At a current density of 1 A g^(−1),the optimal doped sample NHF-0.03 displayed a maximum specific capacity of 640 C g^(−1).The assembled NHF-0.03//AC asymmetric supercapacitor exhibited a maximum power density of 750 W kg^(−1)and a maximum energy density of 55.5 W h kg^(−1).After 10000 charge/discharge cycles,the supercapacitor device preserved 77%of its original capacity at a high current density of 15 A g^(−1),indicating good cycling stability.Overall,the proposed innovative doping strategy looks promising for the enhancement and refinement of materials based on hydroxyfluoride for advanced supercapacitor electrodes.
