【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ...【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.展开更多
Purpose:Derivative chromosomes,resulting from complex structural rearrangements,pose significant challenges in prenatal diagnosis due to their unpredictable inheritance patterns and potential phenotypic consequences.T...Purpose:Derivative chromosomes,resulting from complex structural rearrangements,pose significant challenges in prenatal diagnosis due to their unpredictable inheritance patterns and potential phenotypic consequences.This study evaluates the efficacy of multiple genetic technologies-including karyotyping,fluorescence in situ hybridization(FISH),chromosomal microarray analysis(CMA),and next-generation sequencing(NGS)-in detecting and characterizing derivative chromosomes in prenatal samples.methodology:We analyzed 150 cases of suspected chromosomal abnormalities,comparing detection rates,resolution,and clinical utility across these methods.Results:Our findings demonstrate that integrated multi-technology approaches significantly improve diagnostic accuracy,with NGS-based structural variation analysis achieving the highest detection sensitivity(99.2%)for cryptic rearrangements.Conclusions:Additionally,long-read sequencing(PacBio/Oxford Nanopore)enabled precise breakpoint mapping in 92%of cases,facilitating more accurate genetic counseling.Clinically,this approach enhances risk assessment for fetal anomalies,guides pregnancy management,and improves parental counseling for recurrence risks.展开更多
Background:Precision medicine is an emerging approach for treating pediatric cancer due to its ability to target tumor-specific genetic drivers rather than provide broad and aggressive treatments.The study aimed to ou...Background:Precision medicine is an emerging approach for treating pediatric cancer due to its ability to target tumor-specific genetic drivers rather than provide broad and aggressive treatments.The study aimed to outline the establishment and impact of a Precision Medicine Clinic(PMC)in the setting of pediatric oncology,with the objective of offering targeted treatment options within the institution and creating a scalable model for adoption by other healthcare systems to achieve a wider impact.Methods:Recognizing this need for an individualized approach to treating patients,Cook Children’s Medical Center(CCMC)established a multidisciplinary molecular tumor board in 2019,followed by the launch of an official PMC in 2021.Before this,there was no dedicated place to discuss and evaluate genetic sequencing results.Results:In 2022 and 2023,the PMC discussed 69 patients with a wide variety of oncologic diagnoses.Through the clinic’s efforts,133 genetic variants across 101 genes have been identified,spanning oncogenic pathways related to cell cycling,DNA processing,and cell signaling.Of the sequenced patients,four have received targeted therapy according to recommendations from the PMC.Conclusion:While the PMC continues to evaluate patients and their long-term outcomes,the continually growing PMC at CCMC represents the beginning of the advancement of treating pediatric oncology patients through the interpretation of genetic sequencing results,making actionable targeted treatment recommendations,and continuing to follow the patient’s course of care over time.This additionally provides a framework for starting a PMC that can be adapted for specific clinical needs and implemented broadly.展开更多
Non-small cell lung cancer(NSCLC)accounts for the majority of lung cancer cases and remains the leading cause of cancer-related mortality worldwide.Firstly,this review explores the limitations of conventional therapie...Non-small cell lung cancer(NSCLC)accounts for the majority of lung cancer cases and remains the leading cause of cancer-related mortality worldwide.Firstly,this review explores the limitations of conventional therapies,chemotherapy,radiotherapy,and surgery,focusing on the development of drug resistance and significant toxicity that often hinder their efficacy.Thereafter,advancements in targeted therapies,such as immune checkpoint inhibitors(ICIs)and tyrosine kinase inhibitors(TKIs),are discussed,highlighting their impact on improving outcomes for patients with specific genetic mutations,including c-ros oncogene 1 receptor tyrosine kinase(ROS1),anaplastic lymphoma kinase(ALK),and epidermal growth factor receptor(EGFR).Additionally,the emergence of novel immunotherapies and phytochemicals is examined,emphasizing their potential to overcome therapeutic resistance,particularly in advanced-stage diseases.The review also delves into the role of next-generation sequencing(NGS)in enabling personalized treatment approaches and explores the clinical potential of innovative agents,such as bispecific T-cell engagers(BiTEs)and antibody-drug conjugates(ADCs).Finally,we address the socioeconomic barriers that limit the accessibility of these therapies in low-resource settings and propose future research directions aimed at improving the long-term efficacy and accessibility of these treatments.展开更多
文摘【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
文摘Purpose:Derivative chromosomes,resulting from complex structural rearrangements,pose significant challenges in prenatal diagnosis due to their unpredictable inheritance patterns and potential phenotypic consequences.This study evaluates the efficacy of multiple genetic technologies-including karyotyping,fluorescence in situ hybridization(FISH),chromosomal microarray analysis(CMA),and next-generation sequencing(NGS)-in detecting and characterizing derivative chromosomes in prenatal samples.methodology:We analyzed 150 cases of suspected chromosomal abnormalities,comparing detection rates,resolution,and clinical utility across these methods.Results:Our findings demonstrate that integrated multi-technology approaches significantly improve diagnostic accuracy,with NGS-based structural variation analysis achieving the highest detection sensitivity(99.2%)for cryptic rearrangements.Conclusions:Additionally,long-read sequencing(PacBio/Oxford Nanopore)enabled precise breakpoint mapping in 92%of cases,facilitating more accurate genetic counseling.Clinically,this approach enhances risk assessment for fetal anomalies,guides pregnancy management,and improves parental counseling for recurrence risks.
文摘Background:Precision medicine is an emerging approach for treating pediatric cancer due to its ability to target tumor-specific genetic drivers rather than provide broad and aggressive treatments.The study aimed to outline the establishment and impact of a Precision Medicine Clinic(PMC)in the setting of pediatric oncology,with the objective of offering targeted treatment options within the institution and creating a scalable model for adoption by other healthcare systems to achieve a wider impact.Methods:Recognizing this need for an individualized approach to treating patients,Cook Children’s Medical Center(CCMC)established a multidisciplinary molecular tumor board in 2019,followed by the launch of an official PMC in 2021.Before this,there was no dedicated place to discuss and evaluate genetic sequencing results.Results:In 2022 and 2023,the PMC discussed 69 patients with a wide variety of oncologic diagnoses.Through the clinic’s efforts,133 genetic variants across 101 genes have been identified,spanning oncogenic pathways related to cell cycling,DNA processing,and cell signaling.Of the sequenced patients,four have received targeted therapy according to recommendations from the PMC.Conclusion:While the PMC continues to evaluate patients and their long-term outcomes,the continually growing PMC at CCMC represents the beginning of the advancement of treating pediatric oncology patients through the interpretation of genetic sequencing results,making actionable targeted treatment recommendations,and continuing to follow the patient’s course of care over time.This additionally provides a framework for starting a PMC that can be adapted for specific clinical needs and implemented broadly.
文摘Non-small cell lung cancer(NSCLC)accounts for the majority of lung cancer cases and remains the leading cause of cancer-related mortality worldwide.Firstly,this review explores the limitations of conventional therapies,chemotherapy,radiotherapy,and surgery,focusing on the development of drug resistance and significant toxicity that often hinder their efficacy.Thereafter,advancements in targeted therapies,such as immune checkpoint inhibitors(ICIs)and tyrosine kinase inhibitors(TKIs),are discussed,highlighting their impact on improving outcomes for patients with specific genetic mutations,including c-ros oncogene 1 receptor tyrosine kinase(ROS1),anaplastic lymphoma kinase(ALK),and epidermal growth factor receptor(EGFR).Additionally,the emergence of novel immunotherapies and phytochemicals is examined,emphasizing their potential to overcome therapeutic resistance,particularly in advanced-stage diseases.The review also delves into the role of next-generation sequencing(NGS)in enabling personalized treatment approaches and explores the clinical potential of innovative agents,such as bispecific T-cell engagers(BiTEs)and antibody-drug conjugates(ADCs).Finally,we address the socioeconomic barriers that limit the accessibility of these therapies in low-resource settings and propose future research directions aimed at improving the long-term efficacy and accessibility of these treatments.
