Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blac...Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.展开更多
Objective We aim to explore positive predictive value(PPV)in non-invasive prenatal testing(NIPT)-positive case and investigate the impact of diverse clinical indications and Z-scores on PPV performance.Methods From Ja...Objective We aim to explore positive predictive value(PPV)in non-invasive prenatal testing(NIPT)-positive case and investigate the impact of diverse clinical indications and Z-scores on PPV performance.Methods From January 2021 to June 2024,37891 pregnant women underwent NIPT screening for fetal trisomy 21(T21),trisomy 18(T18)and trisomy 13(T13)a our laboratory using the NextSeq CN500 platform.Positive results were verified through prenatal diagnostic karyotyp analysis and fluorescence in situ hybridisation(FISH)techniques.Results The sensitivity,specificity and PPV were 95.24%99.95%,67.80%for T21;100%,99.97%,56.00%for T18;and 100%,99.97%,16.67%for T13.Across clinical indications,PPV ranged from 0%to 100%for T21 and T18 and 0%to 28.57%for T13.In the T21 group,the predominant proportion of pregnant women(45.76%)exhibited Z-scores between 5 and 10,accompanied by a PPV of 77.78%.For those with Z-scores above 10(23.73%),the PPV was 85.71%.Pregnant women with Z-scores between 3 and 5 exhibited a PPV of 16.67%.In the T18 group,the majority of women(52.00%)exhibited Z-scores ranging from 3 to 5,with a PPV of 33.85%.In the T13 group,all women had Z-scores between 5 and 10,with a PPV of 40.00%.Conclusions NIPT exhibits elevated PPVs for T21 and T18.Moreover,the detection eficacy of NIPT difters acros several clinical indication categories.The PPV performanc of NIPT for T21/T18/T13 is associated with Z-scores.These results provide valuable guidance for clinicians in prenatal consultation and interpretation of NIPT results.展开更多
文摘Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.
文摘Objective We aim to explore positive predictive value(PPV)in non-invasive prenatal testing(NIPT)-positive case and investigate the impact of diverse clinical indications and Z-scores on PPV performance.Methods From January 2021 to June 2024,37891 pregnant women underwent NIPT screening for fetal trisomy 21(T21),trisomy 18(T18)and trisomy 13(T13)a our laboratory using the NextSeq CN500 platform.Positive results were verified through prenatal diagnostic karyotyp analysis and fluorescence in situ hybridisation(FISH)techniques.Results The sensitivity,specificity and PPV were 95.24%99.95%,67.80%for T21;100%,99.97%,56.00%for T18;and 100%,99.97%,16.67%for T13.Across clinical indications,PPV ranged from 0%to 100%for T21 and T18 and 0%to 28.57%for T13.In the T21 group,the predominant proportion of pregnant women(45.76%)exhibited Z-scores between 5 and 10,accompanied by a PPV of 77.78%.For those with Z-scores above 10(23.73%),the PPV was 85.71%.Pregnant women with Z-scores between 3 and 5 exhibited a PPV of 16.67%.In the T18 group,the majority of women(52.00%)exhibited Z-scores ranging from 3 to 5,with a PPV of 33.85%.In the T13 group,all women had Z-scores between 5 and 10,with a PPV of 40.00%.Conclusions NIPT exhibits elevated PPVs for T21 and T18.Moreover,the detection eficacy of NIPT difters acros several clinical indication categories.The PPV performanc of NIPT for T21/T18/T13 is associated with Z-scores.These results provide valuable guidance for clinicians in prenatal consultation and interpretation of NIPT results.
