Ralstonia solanacearum infecting Davana （Artemisia pallens Wall.） from commercial nurseries in India was isolated on modified semi selective media （SMSA）. Here, we report a new host for Ralstonia solanacearum i.e....Ralstonia solanacearum infecting Davana （Artemisia pallens Wall.） from commercial nurseries in India was isolated on modified semi selective media （SMSA）. Here, we report a new host for Ralstonia solanacearum i.e. davana. It has huge demand in medicinal and aromatic industries. Isolate was confirmed as race-l, biovar-3 by morphological, physiological, biochemical and pathogenicity studies. Two sets of primers （OLI 1 ＆ Y2 and Y 1 ＆ Y2） were used in this study. Further, the identity of the isolate was confirmed by serological diagnostic kit obtained from International Potato Research Center, Lima, Peru and single chain variable fragment antibody specific to Ralstonia solanacearum used to confirm the casual organism.展开更多
Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kal...Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kalimantan Islands.Methods:Specimens for light microscopy examination were fixed with warm 70%alcohol,cleared and mounted in lactophenol for wet mounting.Drawings were made with the aid of a drawing tube attached to a Nikon compound microscope.Measurements were given in micrometers(μ) as the average of findings,followed by the range in parentheses,unless otherwise stated.Results:Kalkephalus(Costatus) indicus was found from 7 Ptyas mucosus,Kalicephalus bungari from 2 Naja sputatrix and 1 Kalicephalus(Costatus) indicus and Kalicephalus assimilis found from 1 Ophiophagus hannah.The morphology and measurement of three species of Kalicephalus found in this study were close to those described before.Conclusions:New finding of host of Kalicephalus(Costalus) indicus and Kalicephalus bungari was a snake species of Naja sputatrix.New records of locality were Kalimantan island as the new locality of Kalicephalus assimilis,and Java island was new locality of Kalicephalus(Costatus) indicus.展开更多
The genus Gaeolaelaps Evans & Till from China,including 20 species,is reviewed and an identification key to the Chinese species is provided.G.gillespiei Beaulieu,2009 is reported from China for the first time,with...The genus Gaeolaelaps Evans & Till from China,including 20 species,is reviewed and an identification key to the Chinese species is provided.G.gillespiei Beaulieu,2009 is reported from China for the first time,with redescription and illustrations.Three new mammal hosts are reported (Anourosorex squamipes,Eothenomys miletus and Rattus steni).展开更多
本文描述了从泰国进境的束花凤梨(Tillandsia fasciculata)植株和万代兰(Vanda subconcolor)根系中检疫截获的两种滑刃属(Aphelenchoides)线虫。为明确其分类地位,本研究从形态学和分子生物学两个方面对其进行了系统鉴定。形态观察结果...本文描述了从泰国进境的束花凤梨(Tillandsia fasciculata)植株和万代兰(Vanda subconcolor)根系中检疫截获的两种滑刃属(Aphelenchoides)线虫。为明确其分类地位,本研究从形态学和分子生物学两个方面对其进行了系统鉴定。形态观察结果表明,泰国束花凤梨滑刃线虫种群的形态特征与原始描述的假古氏滑刃线虫(A.pseudogoodeyi)美国佛罗里达草莓(Fragaria×ananassa)种群高度相似,仅体长与虫体最前端至食道-肠交接处长度的比值(b值)存在差异,其余测计值均重叠。泰国万代兰滑刃线虫种群的形态特征与原始描述的苜蓿滑刃线虫(A.medicagus)美国苜蓿(Medicagosativa)种群高度相似,仅雌虫体长与尾长的比值(c值)存在差异,其余测计值均相互重叠。基于近全长的18S r DNA和28S rDNA D2-D3区序列构建了贝叶斯系统发育树。分析显示,在18S rDNA贝叶斯系统发育树中,泰国束花凤梨滑刃线虫种群与其他已报道的假古氏滑刃线虫几个种群聚在很高置信度的独立分支上(后验概率值为1.00),而在28SrDNA贝叶斯系统发育树中,所有假古氏滑刃线虫种群聚在较高置信度的独立分支上(后验概率值为0.98)。泰国万代兰滑刃线虫种群与苜蓿滑刃线虫种群在18S rDNA和28S r DNA D2-D3区贝叶斯系统发育树中,均聚为高置信度的独立分支(后验概率值均为1.00)。综合形态学和分子生物学证据,确定泰国束花凤梨滑刃线虫种群为假古氏滑刃线虫,而万代兰滑刃线虫种群为苜蓿滑刃线虫。这两种线虫均为泰国地理分布新记录种,同时束花凤梨和万代兰也分别是这两种线虫的新寄主记录种。本研究丰富了我们对滑刃属线虫生态、地理以及寄主范围等方面的认识,同时也为针对从泰国进境植物制定有效的植物保护和植物检疫措施提供了科学依据。展开更多
文摘Ralstonia solanacearum infecting Davana （Artemisia pallens Wall.） from commercial nurseries in India was isolated on modified semi selective media （SMSA）. Here, we report a new host for Ralstonia solanacearum i.e. davana. It has huge demand in medicinal and aromatic industries. Isolate was confirmed as race-l, biovar-3 by morphological, physiological, biochemical and pathogenicity studies. Two sets of primers （OLI 1 ＆ Y2 and Y 1 ＆ Y2） were used in this study. Further, the identity of the isolate was confirmed by serological diagnostic kit obtained from International Potato Research Center, Lima, Peru and single chain variable fragment antibody specific to Ralstonia solanacearum used to confirm the casual organism.
文摘Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kalimantan Islands.Methods:Specimens for light microscopy examination were fixed with warm 70%alcohol,cleared and mounted in lactophenol for wet mounting.Drawings were made with the aid of a drawing tube attached to a Nikon compound microscope.Measurements were given in micrometers(μ) as the average of findings,followed by the range in parentheses,unless otherwise stated.Results:Kalkephalus(Costatus) indicus was found from 7 Ptyas mucosus,Kalicephalus bungari from 2 Naja sputatrix and 1 Kalicephalus(Costatus) indicus and Kalicephalus assimilis found from 1 Ophiophagus hannah.The morphology and measurement of three species of Kalicephalus found in this study were close to those described before.Conclusions:New finding of host of Kalicephalus(Costalus) indicus and Kalicephalus bungari was a snake species of Naja sputatrix.New records of locality were Kalimantan island as the new locality of Kalicephalus assimilis,and Java island was new locality of Kalicephalus(Costatus) indicus.
基金supported by the National Natural Science Foundation of China (31501847, 31093430)the Key Research and Development Program of Shandong Province (2016GNC110012, 2017CXGC0207)the Funds of Shandong “Double Tops” Program (SYL2017XTTD06)
文摘The genus Gaeolaelaps Evans & Till from China,including 20 species,is reviewed and an identification key to the Chinese species is provided.G.gillespiei Beaulieu,2009 is reported from China for the first time,with redescription and illustrations.Three new mammal hosts are reported (Anourosorex squamipes,Eothenomys miletus and Rattus steni).
文摘本文描述了从泰国进境的束花凤梨(Tillandsia fasciculata)植株和万代兰(Vanda subconcolor)根系中检疫截获的两种滑刃属(Aphelenchoides)线虫。为明确其分类地位,本研究从形态学和分子生物学两个方面对其进行了系统鉴定。形态观察结果表明,泰国束花凤梨滑刃线虫种群的形态特征与原始描述的假古氏滑刃线虫(A.pseudogoodeyi)美国佛罗里达草莓(Fragaria×ananassa)种群高度相似,仅体长与虫体最前端至食道-肠交接处长度的比值(b值)存在差异,其余测计值均重叠。泰国万代兰滑刃线虫种群的形态特征与原始描述的苜蓿滑刃线虫(A.medicagus)美国苜蓿(Medicagosativa)种群高度相似,仅雌虫体长与尾长的比值(c值)存在差异,其余测计值均相互重叠。基于近全长的18S r DNA和28S rDNA D2-D3区序列构建了贝叶斯系统发育树。分析显示,在18S rDNA贝叶斯系统发育树中,泰国束花凤梨滑刃线虫种群与其他已报道的假古氏滑刃线虫几个种群聚在很高置信度的独立分支上(后验概率值为1.00),而在28SrDNA贝叶斯系统发育树中,所有假古氏滑刃线虫种群聚在较高置信度的独立分支上(后验概率值为0.98)。泰国万代兰滑刃线虫种群与苜蓿滑刃线虫种群在18S rDNA和28S r DNA D2-D3区贝叶斯系统发育树中,均聚为高置信度的独立分支(后验概率值均为1.00)。综合形态学和分子生物学证据,确定泰国束花凤梨滑刃线虫种群为假古氏滑刃线虫,而万代兰滑刃线虫种群为苜蓿滑刃线虫。这两种线虫均为泰国地理分布新记录种,同时束花凤梨和万代兰也分别是这两种线虫的新寄主记录种。本研究丰富了我们对滑刃属线虫生态、地理以及寄主范围等方面的认识,同时也为针对从泰国进境植物制定有效的植物保护和植物检疫措施提供了科学依据。
