Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic...Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia.展开更多
目的探讨神经前体细胞表达下调因子4(NEDD4)基因沉默对膀胱癌细胞株T24和RT4增殖、迁移和侵袭的影响。方法采用脂质体转染技术将合成的三对NEDD4小干扰RNA(NEDD4siRNA1、NEDD4siRNA2、NEDD4siRNA3)分别转染膀胱癌细胞T24和RT4,转染48h...目的探讨神经前体细胞表达下调因子4(NEDD4)基因沉默对膀胱癌细胞株T24和RT4增殖、迁移和侵袭的影响。方法采用脂质体转染技术将合成的三对NEDD4小干扰RNA(NEDD4siRNA1、NEDD4siRNA2、NEDD4siRNA3)分别转染膀胱癌细胞T24和RT4,转染48h后应用RT-PCR法和Western blotting法检测NEDD4的mRNA和蛋白表达,筛选出干扰效率最高的siRNA,并进一步检测转染干扰效率最高的siRNA对膀胱癌细胞增殖、迁移和侵袭的影响;取T24和RT4细胞,设立空白对照组、阴性对照组和实验组,空白对照组为野生型T24和RT4细胞,不予转染;阴性对照组细胞转染Negative Control siRNA,实验组细胞转染NEDD4siRNA-2。转染48h后提取蛋白质,Western blotting法检测NEDD4蛋白及其下游靶蛋白PTEN和p73表达。结果NEDD4的三对siRNA均可下调NEDD4表达,其中NEDD4siRNA2效率最高;与阴性对照相比,实验组T24和RT4细胞增殖、迁移和侵袭能力均下降(P均<0.01)。与阴性对照组相比,实验组NEDD4蛋白及其下游靶蛋白PTEN和p73表达均增加(P均<0.001)。结论NEDD4基因沉默能抑制膀胱癌细胞的增殖、迁移和侵袭。展开更多
文摘Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia.
文摘目的探讨神经前体细胞表达下调因子4(NEDD4)基因沉默对膀胱癌细胞株T24和RT4增殖、迁移和侵袭的影响。方法采用脂质体转染技术将合成的三对NEDD4小干扰RNA(NEDD4siRNA1、NEDD4siRNA2、NEDD4siRNA3)分别转染膀胱癌细胞T24和RT4,转染48h后应用RT-PCR法和Western blotting法检测NEDD4的mRNA和蛋白表达,筛选出干扰效率最高的siRNA,并进一步检测转染干扰效率最高的siRNA对膀胱癌细胞增殖、迁移和侵袭的影响;取T24和RT4细胞,设立空白对照组、阴性对照组和实验组,空白对照组为野生型T24和RT4细胞,不予转染;阴性对照组细胞转染Negative Control siRNA,实验组细胞转染NEDD4siRNA-2。转染48h后提取蛋白质,Western blotting法检测NEDD4蛋白及其下游靶蛋白PTEN和p73表达。结果NEDD4的三对siRNA均可下调NEDD4表达,其中NEDD4siRNA2效率最高;与阴性对照相比,实验组T24和RT4细胞增殖、迁移和侵袭能力均下降(P均<0.01)。与阴性对照组相比,实验组NEDD4蛋白及其下游靶蛋白PTEN和p73表达均增加(P均<0.001)。结论NEDD4基因沉默能抑制膀胱癌细胞的增殖、迁移和侵袭。
