Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limi...Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limited.In this work,optical microangiography(OMAG),a volumetric,label-free imaging technique,is utilized for characterizing,with high resolution,blood perfusion of peripheral nerve in diabetic mice.We demonstrate that OMAG is able to visualize the structure of microvasculature and to quantify the changes of dynamic blood flow and vessel diameters during administration of vessel stimulant in both diabetic and normal mice.The results indicate the potential of OMAG to assess the blood supply of nerve involved in the pathology and treatment of DN.展开更多
基金supported in part by research grants from the National Institutes of Health(R01HL093140,R01EB009682,and R01DC010201)(RKW)the American Heart Association(0855733G)(RKW)the National Institute of Diabetes and Digestive and Kidney Diseases(5 U24 DK076169-04,Pilot and Feasibility Study 09MCG81)(TKB).
文摘Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limited.In this work,optical microangiography(OMAG),a volumetric,label-free imaging technique,is utilized for characterizing,with high resolution,blood perfusion of peripheral nerve in diabetic mice.We demonstrate that OMAG is able to visualize the structure of microvasculature and to quantify the changes of dynamic blood flow and vessel diameters during administration of vessel stimulant in both diabetic and normal mice.The results indicate the potential of OMAG to assess the blood supply of nerve involved in the pathology and treatment of DN.
