Accurate and early evaluation of dental physiological characteristics is essential for effective disease detection and management.Polarization-sensitive optical coherence tomography(PS-OCT)is a noninvasive high-resolu...Accurate and early evaluation of dental physiological characteristics is essential for effective disease detection and management.Polarization-sensitive optical coherence tomography(PS-OCT)is a noninvasive high-resolution imaging technique that can obtain tooth structure and physiological features through intensity,retardation and degree of polarization uniformity(DOPU)images.In this study,we use the information provided by the PS-OCT system,especially the retardation information,to assess the physiological characteristics of teeth.In addition,we introduce a novel parameter—the rate of change of the retardation value(R)—to evaluate the magnitude of birefringence of enamel,which serves as an indicator of enamel health and mineralization.The results demonstrate that PS-OCT not only provides structural detail comparable to microcomputed tomography(microCT),but also yields additional physiological characteristics insights through polarization-based contrast.These findings highlight the strong potential of PS-OCT for early diagnosis,functional evaluation and personalized monitoring in dental care.展开更多
Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment ...Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment and physiological activities of tomato seedlings in seedbeds by controlled experiments.The results indicated that airflow could enhance CO_(2) concentration near the seedling canopy,accelerate water evaporation from the seedling substrate,and reduce fluctuations in the temperature and humidity in microclimate.The photosynthetic rates of leaves at the 4th,7th,and 10th positions in seedlings subjected to airflow increased by 25.04%,8.23%,and 8.47%,respectively,whereas the transpiration rates increased by 15.59%,22.28%,and 13.26%,respectively when compared to the control group.Additionally,the strong seedling index of seedlings treated with airflow and exogenous iron element increased by 26.02%and 31.5%,respectively.Compared to seedlings treated with exogenous iron element,the geometric mean diameter of the pith tissue cells in the stems of seedlings subjected to airflow disturbance was reduced by approximately 18.66%,while the elastic modulus and bending strength of the stems increased by 10.01%and 5.89%,respectively.Similarly,the volume of root tissue cells decreased by 19.22%,but the elastic modulus of the roots increased by 6.46%.This study confirms that airflow significantly enhances seedling resilience to abiotic stress,yielding similar or better outcomes than exogenous iron application.It provides both theoretical and practical support for using airflow disturbance as a green technology for cultivating robust seedlings.展开更多
Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the elec...Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the electrode-neural interface.Methods:A prospective observational study involving 56 pediatric patients underwent cochlear implantation with Cochlear Nucleus devices.Intraoperative polarized impedance and electrically evoked compound action potential(ECAP)threshold were recorded across all 1232 electrodes using AutoNRT software.Eight electrodes with open-or short-circuit were excluded,leaving 1,224 for analysis.Impedance values were categorized by cochlear region(basal,middle,apical),and electrodes with elevated impedance(10-20 kΩ)were analyzed for regional distribution and clinical relevance.Data were analyzed for spatial patterns and correlation with the ECAP threshold profiles.Results:A consistent basal-to-apical increase in impedance was observed(7.7±1.9,9.2±1.4,10.8±1.5 kΩ;p<0.001).Impedance and ECAP threshold were weakly correlated(ρ=-0.20,p<0.001;β=-1.26,p<0.001),with a positive association in the apical region(ρ=0.12,p=0.048).Electrodes with higher impedance(1020 kΩ)were less likely to show elevated or absent TNRT(OR=0.175,p=0.02).The impedance gradient persisted across age groups and was significantly correlated with ECAP threshold patterns.Conclusion:Intraoperative impedance monitoring reveals a strong and physiologically consistent gradient,with higher values in apical electrodes.This gradient reflects anatomical and tissue interface variations,which may offer a valuable physiological indicator for intraoperative electrode positioning and neural interface integrity.展开更多
Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in...Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.展开更多
Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty qua...Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty quantification to enhance the reliability of predictions.Physiological signals,including Electrocardiogram(ECG),Galvanic Skin Response(GSR),and Electroencephalogram(EEG),were transformed into image representations and analyzed using pretrained deep neu-ral networks.The extracted features were classified through a feedforward neural network,and prediction reliability was assessed using uncertainty quantification techniques such as Monte Carlo Dropout(MCD),model ensembles,and combined approaches.Evaluation metrics included standard measures(sensitivity,specificity,precision,and accuracy)along with uncertainty-aware metrics such as uncertainty sensitivity and uncertainty precision.Across all evaluations,ECG-based models consistently demonstrated strong performance.The findings indicate that combining multimodal physi-ological signals,Transfer Learning(TL),and uncertainty quantification can significantly improve both the accuracy and trustworthiness of fatigue detection systems.This approach supports the development of more reliable driver assistance technologies aimed at preventing fatigue-related accidents.展开更多
AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from thr...AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from three groups,including control group(normal oxygen),hypoxic group(hypoxia at 37℃,1%O_(2),5%CO_(2),and 94%N_(2)),and LBP group(hypoxic group with LBP 100μg/mL).In vivo experiments,C57 mice were divided into three groups:control group(normal rearing group),the oxygen-induced ischemic retinopathy(OIR)group,and the OIR with 50 mg/kg LBP group.Retinal neovascularization was observed by fluorescein angiography and quantified.Retinal thickness was evaluated by Hematoxylin and eosin(HE)stain.The expression of epidermal growth factor receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),mammalian target of rapamycin(mTOR),phosphorylated mammalian target of rapamycin(p-mTOR),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS),and tumor necrosis factor-α(TNF-α)in each group were analyzed by Western blot.IL-1βlevel in retina was analyzed using immunohistochemical staining.RESULTS:The increased area of neovascular clusters in OIR mice was significantly decreased by LBP.Retinal thickness of OIR mice was significantly thinner compared with normal oxygenated mice and was increased in LBP group.Compared with those in the hypoxic groups,Western blotting of HRECs and retinal tissues revealed that the expression of EGFR,PI3K,p-mTOR,p-AKT,IL-1β,iNOS,and TNF-αdecreased in the LBP group but was still greater than that in control group.Moreover,IL-1βwas reduced in retinal sections treated with LBP.In the scratch test,the cell migration of the hypoxic group was significantly greater than that of the control group,while LBP treatment attenuated this increase in migration.CONCLUSION:LBP reduces retinal neovascularization and inflammation in vivo and inhibits the migration of HRECs in vitro by regulating the EGFR/PI3K/Akt/mTOR signaling pathway.展开更多
Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negate...Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.展开更多
Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In...Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.展开更多
Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural count...Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.展开更多
AIM:To repor t the 24mo outcomes of vascular endothelial growth factor(VEGF)inhibitors for myopic choroidal neovascularization(mCNV)in routine clinical practice and simultaneously evaluated the real-world safety.METHO...AIM:To repor t the 24mo outcomes of vascular endothelial growth factor(VEGF)inhibitors for myopic choroidal neovascularization(mCNV)in routine clinical practice and simultaneously evaluated the real-world safety.METHODS:The patients who received intravitreal injections of VEGF inhibitors of either ranibizumab(0.5 mg)or conbercept(0.5 mg)for mCNV were analyzed from 1 January 2017 to 1 January 2022.The primary outcome variables were mean change in best-corrected visual acuity(BCVA)and central macular thickness(CMT)changes.The secondary outcome variables included IOP changes,the period of mCNV re-treatment,and ocular adverse events.RESULTS:Totally 83 patients aged 56.40±15.36y with axial length 29.67±2.09 mm were included.In visual acuity,the mean logMAR BCVA at baseline was 0.81±0.43.After the initial improvement at 1,3,and 6mo(P<0.05),from month 12 onwards,no statistical difference compared to baseline was found.The mean CMT from 1mo onwards had a statistically significant decrease compared with baseline CMT(P<0.05).The regression model showed better baseline BCVA and thicker baseline CMT,significantly associated with the final outcomes.In univariate analysis,choosing 3+pro re nata(PRN)as the initial injection treatment regimen was associated with better BCVA at 24mo[hazard ratio(HR)=-0.65,95%CI:-1.23,-0.07,P=0.048].However,the difference was not significant in multivariate analysis(HR=-0.59,95%CI:-1.21,0.03,P=0.089).Regarding mCNV recurrence,the mean period(P=0.725)and the proportion of mCNV reactivation(P=1.00)were similar between ranibizumab and conbercept.Kaplan-Meier plot also analyzed that the median time of re-injection was not significantly different among gender,drug,and initial injection treatment regimen.No systemic adverse events related to the therapy were observed.CONCLUSION:BCVA gains achieved by the end of our study maintain generally sustained at the 24-mo follow-up.The findings also indicate that ranibizumab and conbercept demonstrate comparable efficacy and safety profiles.Additionally,intravitreal anti-VEGF therapy using 1+PRN regimen,offers certain advantages in both efficacy and cost-effectiveness.展开更多
Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves t...Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.展开更多
Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among...Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.展开更多
The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness...The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.展开更多
Erratum to:Current Medical Science https://doi.org/10.1007/s11596-025-00114-3In the originally published article(https://doi.org/10.1007/s11596-025-00114-3),the label of the vertical axis in Fig.2b was incorrect.Inste...Erratum to:Current Medical Science https://doi.org/10.1007/s11596-025-00114-3In the originally published article(https://doi.org/10.1007/s11596-025-00114-3),the label of the vertical axis in Fig.2b was incorrect.Instead of“24 h cell viability(%)”,it should be corrected to“Proliferation rate(%)”.The authors apologize for this error and state that this does not change the scientific conclusions of the article.展开更多
Introduction Choroidal neovascularization(CNV)associated with ocular inflammatory disease,also known as inflammatory CNV(iCNV),is a rare complication that can lead to severe vision loss in patients with infectious and...Introduction Choroidal neovascularization(CNV)associated with ocular inflammatory disease,also known as inflammatory CNV(iCNV),is a rare complication that can lead to severe vision loss in patients with infectious and noninfectious uveitis(1-3).After pathologic myopia,ocular inflammatory disease is a frequently implicated cause of CNV in patients younger than 50 years old(4).展开更多
Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A random...Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.展开更多
Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives wer...Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.展开更多
AIM:To quantitatively assess central macular thickness(CMT),macular neovascularization(MNV)area,vascular tortuosity(VT),and vascular dispersion(VDisp)in neovascular age-related macular degeneration(nAMD),type 1 and ty...AIM:To quantitatively assess central macular thickness(CMT),macular neovascularization(MNV)area,vascular tortuosity(VT),and vascular dispersion(VDisp)in neovascular age-related macular degeneration(nAMD),type 1 and type 2 MNV,by means of optical coherence tomography(OCT)and OCT angiography(OCTA)techniques.METHODS:In this retrospective and observational case series,patients were classified into type 1 or type 2 MNV groups.A comprehensive panel of OCT and OCTA metrics was evaluated,including CMT,MNV area,VT,and VDisp.All subjects underwent a standardized intravitreal conbercept(IVC)regimen[3+pro re nata(PRN)]with a 12-month follow-up.MNV area was obtained by manual measurements with OCTA software,and VT and VDisp were calculated by automated analysis with Image J software.RESULTS:A total of 101 participants were included,with 51 patients in the type 1 MNV group(mean age 67.32±9.12y)and 50 patients in the type 2 MNV group(mean age 64.74±5.21y).The mean number of IVC injections was 3.98±1.53 for type 1 MNV and 3.73±0.81 for type 2 MNV.Both subtypes exhibited significant improvements in visual acuity,accompanied by marked reductions in CMT and MNV area(P<0.05)at 12mo after treatment.In type 2 MNV,VT significantly decreased(P<0.05),whereas no significant change was observed in VT for type 1 MNV.VDisp did not significantly changed in either sybtypes.Moreover,in type 1 MNV,final best-corrected visual acuity(BCVA)using logMAR correlated positively with both pre-and post-treatment CMT,while in type 2 MNV,a significant positive correlation was found between the number of injections and final CMT.CONCLUSION:This study shows that conbercept treatment significantly improves visual acuity and macular structure in both type 1 and type 2 MNV with reductions in CMT and MNV area.The significant reduction in VT in type 2 MNV suggests its potential as a biomarker for disease activity.The findings imply the quantitative assessment useful for the stratification,prognostication,and personalized management of MNV in nAMD.展开更多
AIM:To investigate the choroidal vascular index(CVI)and the choroidal structural changes beyond the subfoveal area(analyzed across a 20 mm×24 mm scanning area)in eyes with chronic central serous chorioretinopathy...AIM:To investigate the choroidal vascular index(CVI)and the choroidal structural changes beyond the subfoveal area(analyzed across a 20 mm×24 mm scanning area)in eyes with chronic central serous chorioretinopathy(cCSC)eyes with macular neovascularization(MNV)using ultra-widefield swept-source optical coherence tomography angiography(UWF SS-OCTA).METHODS:This retrospective comparative study included 46 cCSC with MNV eyes(With MNV group),52 cCSC without MNV eyes(Without MNV group),and 40 age-matched healthy controls.UWF SS-OCTA imaging with a 20 mm×24 mm protocol was used to quantify CVI across 9 subfields(superotemporal,superior,superonasal,temporal,central,nasal,inferotemporal,inferior,and inferonasal).The CVI was compared among the groups.RESULTS:With MNV group demonstrated significantly older mean age than Without MNV group(56.2±6.1 vs 47.5±8.6y,P<0.001).The CVI was significantly lower in the With MNV group than in the Without MNV group,except in the superotemporal,superior,and temporal regions(all P<0.05).Notably,despite MNV-associated CVI reductions,the With MNV group maintained a higher CVI than the control group in all 5 subfields(superior,temporal,central,inferior,and inferonasal;all P<0.05).In the central region,CONCLUSION:CVI decreases,and choroidal structural changes extend beyond the subfoveal area in cCSC with MNV eyes,providing with an imaging evidence for the important role of choroidal ischemia in the pathogenesis of MNV in cCSC.展开更多
Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in...Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in the speciation of Pinus densata,a keystone forest species on the southeastern Tibetan Plateau.By creating artificialinterspecificF1s and a long-term common garden experiment on the plateau,we provide in situ assessments on 44 growth and physiological traits across four seasons,along with RNA sequencing.We found significantphenotypic divergence between P.densata and its putative parental species P.tabuliformis and P.yunnanensis,with P.densata demonstrating superior growth and dynamic balance between photosynthesis and photoprotection.The F1s closely resembled P.densata in most traits.Gene expression revealed 19%–10%of 34,000 examined genes as differentially expressed in P.densata and F1s relative to mid-parent expression values.Both additive(4%)and non-additive gene actions(5%–6%in F1s,10%–12%in P.densata)were common,while transgressive expression occurred more frequently in the stabilized natural hybrids,illustrating transcriptomic reprogramming brought by hybridization and further divergence by natural selection.We provide compelling evidence for hybridization-derived phenotypic divergence at both physiological and gene expression levels that could have contributed to the adaptation of P.densata to high plateau habitat where both parental species have low fitness.The altered physiology and gene expression in hybrids serve both as a substrate for novel ecological adaptation and as a mechanism for the initiation of reproductive isolation.展开更多
基金supported by the National Natural Science Foundation of China(Nos.62375144 and 12404345)"the Fundamental Research Funds for the Central Universities",Nankai University(No.63241331).
文摘Accurate and early evaluation of dental physiological characteristics is essential for effective disease detection and management.Polarization-sensitive optical coherence tomography(PS-OCT)is a noninvasive high-resolution imaging technique that can obtain tooth structure and physiological features through intensity,retardation and degree of polarization uniformity(DOPU)images.In this study,we use the information provided by the PS-OCT system,especially the retardation information,to assess the physiological characteristics of teeth.In addition,we introduce a novel parameter—the rate of change of the retardation value(R)—to evaluate the magnitude of birefringence of enamel,which serves as an indicator of enamel health and mineralization.The results demonstrate that PS-OCT not only provides structural detail comparable to microcomputed tomography(microCT),but also yields additional physiological characteristics insights through polarization-based contrast.These findings highlight the strong potential of PS-OCT for early diagnosis,functional evaluation and personalized monitoring in dental care.
基金supported by an International Cooperation Key Plan of Shaanxi Province(Grant No.2022KWZ-12)an Agricultural Science Innovation and Transformation Project of Shaanxi Province[Grant No.NYKJ-2022-YL(XN)12]a High-End Foreign Expert Recruitment Program(Grant No.G2022172006L).
文摘Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation.In this study,an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment and physiological activities of tomato seedlings in seedbeds by controlled experiments.The results indicated that airflow could enhance CO_(2) concentration near the seedling canopy,accelerate water evaporation from the seedling substrate,and reduce fluctuations in the temperature and humidity in microclimate.The photosynthetic rates of leaves at the 4th,7th,and 10th positions in seedlings subjected to airflow increased by 25.04%,8.23%,and 8.47%,respectively,whereas the transpiration rates increased by 15.59%,22.28%,and 13.26%,respectively when compared to the control group.Additionally,the strong seedling index of seedlings treated with airflow and exogenous iron element increased by 26.02%and 31.5%,respectively.Compared to seedlings treated with exogenous iron element,the geometric mean diameter of the pith tissue cells in the stems of seedlings subjected to airflow disturbance was reduced by approximately 18.66%,while the elastic modulus and bending strength of the stems increased by 10.01%and 5.89%,respectively.Similarly,the volume of root tissue cells decreased by 19.22%,but the elastic modulus of the roots increased by 6.46%.This study confirms that airflow significantly enhances seedling resilience to abiotic stress,yielding similar or better outcomes than exogenous iron application.It provides both theoretical and practical support for using airflow disturbance as a green technology for cultivating robust seedlings.
文摘Objective:To investigate the spatial gradient of intraoperative impedance across the cochlear electrode array in pediatric cochlear implant recipients and assess its potential as a physiological indicator for the electrode-neural interface.Methods:A prospective observational study involving 56 pediatric patients underwent cochlear implantation with Cochlear Nucleus devices.Intraoperative polarized impedance and electrically evoked compound action potential(ECAP)threshold were recorded across all 1232 electrodes using AutoNRT software.Eight electrodes with open-or short-circuit were excluded,leaving 1,224 for analysis.Impedance values were categorized by cochlear region(basal,middle,apical),and electrodes with elevated impedance(10-20 kΩ)were analyzed for regional distribution and clinical relevance.Data were analyzed for spatial patterns and correlation with the ECAP threshold profiles.Results:A consistent basal-to-apical increase in impedance was observed(7.7±1.9,9.2±1.4,10.8±1.5 kΩ;p<0.001).Impedance and ECAP threshold were weakly correlated(ρ=-0.20,p<0.001;β=-1.26,p<0.001),with a positive association in the apical region(ρ=0.12,p=0.048).Electrodes with higher impedance(1020 kΩ)were less likely to show elevated or absent TNRT(OR=0.175,p=0.02).The impedance gradient persisted across age groups and was significantly correlated with ECAP threshold patterns.Conclusion:Intraoperative impedance monitoring reveals a strong and physiologically consistent gradient,with higher values in apical electrodes.This gradient reflects anatomical and tissue interface variations,which may offer a valuable physiological indicator for intraoperative electrode positioning and neural interface integrity.
基金supported by the National Natural Science Foundation of China(Nos.42077367 and 21677123).
文摘Foliar uptake of airborne metal(loid)s plays a crucial role in metal(loid)accumulation in plant organs and is influ-enced by the size and emission sources of aerosols.Given the high enrichment of toxic metal(loid)s in submicron-scale particulates(PM1),this study established a PM1 exposure system to examine airborne metal(loid)accu-mulation and foliar physiological responses in Oryza sativa L.The results showed that the concentrations of Cu,Zn,As,Pb,and Cd in the leaves and grains were influenced not only by the airborne metal(loid)levels but also by the specific nature of the PM1 particles.The quantitative model for PM1-associated Pb entry into leaf tissue indicated that foliar Pb accumulation was primarily driven by particle adhesion,followed by hydrophilic pene-tration and trans-stomatal liquid film migration,accounting for 87%–89%of the total accumulation.The strong hygroscopicity and high Pb activity of PM1 emitted from waste incineration(WI)increased the Pb absorption coefficient via the hydrophilic and liquid film migration pathway.In contrast,the high hydrophobicity of PM1 from coal burning(CB)led to greater retention of Pb on leaf surfaces.Both foliar reactive oxygen metabolism and photosynthesis indices were sensitive to air pollution.Foliar metal(loid)accumulation and airborne PM1 concentration accounted for the variance in physiological responses in rice leaves.Our results also indicated that Pb was the key element in PM1 emissions from both coal burning(CB)and waste incineration(WI)responsible for significant physiological changes in rice leaves.
基金the Australian Research Council Discovery Projects funding scheme(DP190102181,DP210101465).
文摘Accurate detection of driver fatigue is essential for improving road safety.This study investigates the effectiveness of using multimodal physiological signals for fatigue detection while incorporating uncertainty quantification to enhance the reliability of predictions.Physiological signals,including Electrocardiogram(ECG),Galvanic Skin Response(GSR),and Electroencephalogram(EEG),were transformed into image representations and analyzed using pretrained deep neu-ral networks.The extracted features were classified through a feedforward neural network,and prediction reliability was assessed using uncertainty quantification techniques such as Monte Carlo Dropout(MCD),model ensembles,and combined approaches.Evaluation metrics included standard measures(sensitivity,specificity,precision,and accuracy)along with uncertainty-aware metrics such as uncertainty sensitivity and uncertainty precision.Across all evaluations,ECG-based models consistently demonstrated strong performance.The findings indicate that combining multimodal physi-ological signals,Transfer Learning(TL),and uncertainty quantification can significantly improve both the accuracy and trustworthiness of fatigue detection systems.This approach supports the development of more reliable driver assistance technologies aimed at preventing fatigue-related accidents.
基金Supported by the Tianjin Health Research Project(No.ZC20069No.TJWJ2022MS040)+1 种基金the Foundation of the Committee of Integrated Traditional Chinese and Western Medicine(No.2021011)the Science and Technology Foundation of Tianjin Eye Hospital(No.YKYB1901).
文摘AIM:To explore the effect and mechanism of Lycium barbarum polysaccharide(LBP)inhibiting retinal neovascularization.METHODS:In vitro tests were performed on human retinal microvascular endothelial cells(HRECs)from three groups,including control group(normal oxygen),hypoxic group(hypoxia at 37℃,1%O_(2),5%CO_(2),and 94%N_(2)),and LBP group(hypoxic group with LBP 100μg/mL).In vivo experiments,C57 mice were divided into three groups:control group(normal rearing group),the oxygen-induced ischemic retinopathy(OIR)group,and the OIR with 50 mg/kg LBP group.Retinal neovascularization was observed by fluorescein angiography and quantified.Retinal thickness was evaluated by Hematoxylin and eosin(HE)stain.The expression of epidermal growth factor receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),mammalian target of rapamycin(mTOR),phosphorylated mammalian target of rapamycin(p-mTOR),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS),and tumor necrosis factor-α(TNF-α)in each group were analyzed by Western blot.IL-1βlevel in retina was analyzed using immunohistochemical staining.RESULTS:The increased area of neovascular clusters in OIR mice was significantly decreased by LBP.Retinal thickness of OIR mice was significantly thinner compared with normal oxygenated mice and was increased in LBP group.Compared with those in the hypoxic groups,Western blotting of HRECs and retinal tissues revealed that the expression of EGFR,PI3K,p-mTOR,p-AKT,IL-1β,iNOS,and TNF-αdecreased in the LBP group but was still greater than that in control group.Moreover,IL-1βwas reduced in retinal sections treated with LBP.In the scratch test,the cell migration of the hypoxic group was significantly greater than that of the control group,while LBP treatment attenuated this increase in migration.CONCLUSION:LBP reduces retinal neovascularization and inflammation in vivo and inhibits the migration of HRECs in vitro by regulating the EGFR/PI3K/Akt/mTOR signaling pathway.
基金funded by the National Natural Science Foundation of China(Grant No.32201896)the Zhejiang Province Key Research and Development Plan Project,China(Grant No.2022C02034)the National Modern Agricultural Industrial Technology System Construction Project,China(Grant No.CARS-01-21).
文摘Rice yield is heavily reliant on the number of spikelets per panicle,a factor determined by the processes of spikelet differentiation and degeneration.In rice cultivars with large panicles,spikelet degeneration negates the advantages of large panicle and constrains yield potential.Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration,with the sensitive period for this process commencing approximately 15‒20 d before panicle heading.Notable positional variations occur within the panicle,with significantly higher spikelet degeneration rates at the basal than at the upper positions.An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress.Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport,which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation.Sucrose serves as the main carbohydrate source for spikelet development,demonstrating an apical dominance pattern that favors spikelet formation.However,under abiotic stress,the inhibition of sucrose decomposition,rather than sucrose transport impairment,predominantly contributes to aggravated spikelet degeneration at the basal panicle positions.Brassinolide and auxin have a significant relationship with spikelet formation,potentially mediating apical dominance.Specifically,brassinolide enhances sucrose accumulation and utilization,thereby alleviating spikelet degeneration.At present,the mechanisms underlying rice spikelet degeneration have not been fully revealed,and the joint effects of hormones,carbohydrates,and carbon and nitrogen metabolism on this process require further investigation.To reduce the spikelet degeneration,the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’resilience to abiotic stress.An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.
基金supported by the National Natural Science Foundation of China(52127801)Postdoctoral Fellowship Program of CPSF under Grant Number GZC20231545,China Postdoctoral Science Foundation(2024T170557 and 2023M742224)+1 种基金Shanghai Post-doctoral Excellence Program(No.2023440)City University of Hong Kong Donation Grants(DON-RMG No.9229021 and 9220061).
文摘Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.
基金supported by the National Natural Science Foundation of China(NSFC,31971413)to D.L.
文摘Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.
基金Supported by Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-037A).
文摘AIM:To repor t the 24mo outcomes of vascular endothelial growth factor(VEGF)inhibitors for myopic choroidal neovascularization(mCNV)in routine clinical practice and simultaneously evaluated the real-world safety.METHODS:The patients who received intravitreal injections of VEGF inhibitors of either ranibizumab(0.5 mg)or conbercept(0.5 mg)for mCNV were analyzed from 1 January 2017 to 1 January 2022.The primary outcome variables were mean change in best-corrected visual acuity(BCVA)and central macular thickness(CMT)changes.The secondary outcome variables included IOP changes,the period of mCNV re-treatment,and ocular adverse events.RESULTS:Totally 83 patients aged 56.40±15.36y with axial length 29.67±2.09 mm were included.In visual acuity,the mean logMAR BCVA at baseline was 0.81±0.43.After the initial improvement at 1,3,and 6mo(P<0.05),from month 12 onwards,no statistical difference compared to baseline was found.The mean CMT from 1mo onwards had a statistically significant decrease compared with baseline CMT(P<0.05).The regression model showed better baseline BCVA and thicker baseline CMT,significantly associated with the final outcomes.In univariate analysis,choosing 3+pro re nata(PRN)as the initial injection treatment regimen was associated with better BCVA at 24mo[hazard ratio(HR)=-0.65,95%CI:-1.23,-0.07,P=0.048].However,the difference was not significant in multivariate analysis(HR=-0.59,95%CI:-1.21,0.03,P=0.089).Regarding mCNV recurrence,the mean period(P=0.725)and the proportion of mCNV reactivation(P=1.00)were similar between ranibizumab and conbercept.Kaplan-Meier plot also analyzed that the median time of re-injection was not significantly different among gender,drug,and initial injection treatment regimen.No systemic adverse events related to the therapy were observed.CONCLUSION:BCVA gains achieved by the end of our study maintain generally sustained at the 24-mo follow-up.The findings also indicate that ranibizumab and conbercept demonstrate comparable efficacy and safety profiles.Additionally,intravitreal anti-VEGF therapy using 1+PRN regimen,offers certain advantages in both efficacy and cost-effectiveness.
基金The Basic Scientific Research Project of Colleges and Universities in 2022:HRV and Brain Electrophysiological Mechanism of Electromyography Biofeedback in Improving Depression and Anxiety of College Students(Project No.:LJKMZ20221274)。
文摘Biofeedback is a behavioral intervention technology based on psychophysiology,which uses various electronic devices to collect the physiological activities of individuals in real time and provides them to themselves through various ways,so that people can actively change their own physiological changes when they perceive the changes in some physiological functions of their own,and consciously control the physiological functions of various aspects of the autonomic nervous system within a fixed range.It can play a role in improving physical health status,preventing and treating some somatic diseases.Studies have shown that biofeedback relaxation training can make the training subjects learn to regulate their own physiological functions and psychological activities,improve negative emotional states such as depression and anxiety by regulating the body function,and can also be used to assist in the treatment of other clinical diseases.As a new psychological intervention,biofeedback relaxation training has great development potential and can be better applied to the prevention and treatment of physical and mental diseases in the general population in the future.
文摘Morpho-physiological evaluation of a crop’s genetic resources is necessary to find possible genotypes to include in breeding initiatives.The objective of this study was to identify heat-tolerant wheat genotypes among 36 mutant lines using morpho-physiological indices.Seedlings of mutant lines and check varieties were grown under both normal(control)and heat-stress conditions in growth chambers.Data were recorded on root-shoot parameters(length,fresh weight,dry weight,and ratio),relative water content(RWC),stability of cell membrane,pigment content,and chlorophyll fluorescence.Two-way analysis of variance showed significant(p<0.01,p<0.001)variation among 15 morpho-physiological features in both growing conditions.Correlation studies showed that pigment-specific indices(total chl,chl a,chl b,carotenoid,Fv/Fm—chlorophyll fluorescence)had significant positive correlations with rootshoot fresh weight(RFW,SFW)and root-shoot dry weight(RDW,SDW),and negative association with relative water content,stability of cell membrane at both conditions.Hierarchical cluster analysis revealed three primary clusters where cluster III(18 genotypes)showed minimal changes in studied characters than cluster I(11 genotypes)and cluster II(13 genotypes).Results obtained from this study revealed that the identified heat-sensitive(30-2 SSH)and heat-tolerant genotypes(27-2(4),29-2(2),and 30-2 SSH)of wheat might provide useful breeding materials for enhancing wheat yield even more in this period of uncertain climate change.
基金Supported by National Natural Science Foundation of China(Grant Nos.52375279,52175001)Beijing Natural Science Foundation(Grant No.3252002).
文摘The abundant muscle tissues of the forearm determine the movements of the wrist,hand and fingers together.However,linking wrist kinematics and forearm muscle activation is still a challenging.There may exist blindness in the rehabilitation therapy of forearm muscles,due to the lack of the physiological characteristics of muscle activation and sequences.An armband with eight channels was used to collect surface electromyographic signals(sEMGs)of a specific section of the forearm under the different wrist movements,palm postures,and external loads,based on the image of magnetic resonance imaging(MRI).The collected cross-sectional muscles covered almost all surface muscles.The muscle activation could be expressed clearly by enveloping the sEMG signals of 8 muscles within a single cycle.The root mean square(RMS)and the average peak value V_(P) were used to evaluate the activation intensities of dominant muscles.The activation sequences and the absolute times of dominant muscles were obtained from the envelopes of their raw sEMGs,and not influenced by the palm postures and external loads.In addition,their RMS and V_(P) under each wrist movement increased approximate linearly with external loads.The corresponding contribution ratios were first calculated to evaluate the role played by each muscle.The well-defined data of forearm muscles could provide standard references for the rehabilitation therapy of forearm muscles.
文摘Erratum to:Current Medical Science https://doi.org/10.1007/s11596-025-00114-3In the originally published article(https://doi.org/10.1007/s11596-025-00114-3),the label of the vertical axis in Fig.2b was incorrect.Instead of“24 h cell viability(%)”,it should be corrected to“Proliferation rate(%)”.The authors apologize for this error and state that this does not change the scientific conclusions of the article.
文摘Introduction Choroidal neovascularization(CNV)associated with ocular inflammatory disease,also known as inflammatory CNV(iCNV),is a rare complication that can lead to severe vision loss in patients with infectious and noninfectious uveitis(1-3).After pathologic myopia,ocular inflammatory disease is a frequently implicated cause of CNV in patients younger than 50 years old(4).
文摘Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.
基金funded by the‘National Key R&D Program Subject of China’(No.2021YFD1100301)the post subsidy project of National Key R&D Program,and the Guizhou Modern Agriculture Research System(GZMARS)-Plateau characteristic vegetable industry.
文摘Chive(Allium ascalonicum L.),a seeding-vernalization-type vegetable,is prone to bolting.To explore the physiological and molecular mechanisms of its bolting,bolting-prone(‘BA’)and bolting-resistant(‘WA’)chives were sampled at the vegetative growth,floral bud differentiation,and bud emergence stages.No bolting was observed in bolting-resistant‘WA’on the 130th day after planting,whereas the bolting reached 39.22%in bolting-prone‘BA’,which was significantly higher than that of‘WA’.The contents of gibberellins,abscisic acid,and zeatin riboside after floral bud differentiation in‘WA’were significantly less than in‘BA’,whereas the indoleacetic acid content in‘WA’was significantly higher than that in‘BA’before and after floral bud differentiation.The soluble sugar content and nitrate reductase activity in‘BA’were significantly higher than those in‘WA’before and during floral bud differentiation periods.However,they were significantly lower in‘BA’compared with in‘WA’after bolting due to the nutrient consumption required by reproductive growth.A transcriptome analysis determined that the differentially expressed genes related to bolting tolerance were enriched in the terms‘photoperiodism,flowering’,‘auxin-activated signaling pathway’,‘gibberellic acid mediated signaling pathway’,and‘carbohydrate metabolic process’,and this was generally consistent with the physiological data.Additionally,12 key differentially expressed genes(including isoform_203018,isoform_481005,isoform_716975,and isoform_564877)related to bolting tolerance were investigated.This research provides new information for breeding bolting-tolerant chives.
基金Supported by Natural Science Foundation of Shandong Province(No.ZR2023MH363)Bethune Langmu Young Scholars Research Fund Project(No.BJ-LM2021007J).
文摘AIM:To quantitatively assess central macular thickness(CMT),macular neovascularization(MNV)area,vascular tortuosity(VT),and vascular dispersion(VDisp)in neovascular age-related macular degeneration(nAMD),type 1 and type 2 MNV,by means of optical coherence tomography(OCT)and OCT angiography(OCTA)techniques.METHODS:In this retrospective and observational case series,patients were classified into type 1 or type 2 MNV groups.A comprehensive panel of OCT and OCTA metrics was evaluated,including CMT,MNV area,VT,and VDisp.All subjects underwent a standardized intravitreal conbercept(IVC)regimen[3+pro re nata(PRN)]with a 12-month follow-up.MNV area was obtained by manual measurements with OCTA software,and VT and VDisp were calculated by automated analysis with Image J software.RESULTS:A total of 101 participants were included,with 51 patients in the type 1 MNV group(mean age 67.32±9.12y)and 50 patients in the type 2 MNV group(mean age 64.74±5.21y).The mean number of IVC injections was 3.98±1.53 for type 1 MNV and 3.73±0.81 for type 2 MNV.Both subtypes exhibited significant improvements in visual acuity,accompanied by marked reductions in CMT and MNV area(P<0.05)at 12mo after treatment.In type 2 MNV,VT significantly decreased(P<0.05),whereas no significant change was observed in VT for type 1 MNV.VDisp did not significantly changed in either sybtypes.Moreover,in type 1 MNV,final best-corrected visual acuity(BCVA)using logMAR correlated positively with both pre-and post-treatment CMT,while in type 2 MNV,a significant positive correlation was found between the number of injections and final CMT.CONCLUSION:This study shows that conbercept treatment significantly improves visual acuity and macular structure in both type 1 and type 2 MNV with reductions in CMT and MNV area.The significant reduction in VT in type 2 MNV suggests its potential as a biomarker for disease activity.The findings imply the quantitative assessment useful for the stratification,prognostication,and personalized management of MNV in nAMD.
基金Supported by General Program of National Natural Science Foundation of China(No.82471110)National Key Research and Development Program of China(No.2022YFC2502805)Postdoctoral Foundation of General Hospital of Central Theater Command(No.20210517KY04).
文摘AIM:To investigate the choroidal vascular index(CVI)and the choroidal structural changes beyond the subfoveal area(analyzed across a 20 mm×24 mm scanning area)in eyes with chronic central serous chorioretinopathy(cCSC)eyes with macular neovascularization(MNV)using ultra-widefield swept-source optical coherence tomography angiography(UWF SS-OCTA).METHODS:This retrospective comparative study included 46 cCSC with MNV eyes(With MNV group),52 cCSC without MNV eyes(Without MNV group),and 40 age-matched healthy controls.UWF SS-OCTA imaging with a 20 mm×24 mm protocol was used to quantify CVI across 9 subfields(superotemporal,superior,superonasal,temporal,central,nasal,inferotemporal,inferior,and inferonasal).The CVI was compared among the groups.RESULTS:With MNV group demonstrated significantly older mean age than Without MNV group(56.2±6.1 vs 47.5±8.6y,P<0.001).The CVI was significantly lower in the With MNV group than in the Without MNV group,except in the superotemporal,superior,and temporal regions(all P<0.05).Notably,despite MNV-associated CVI reductions,the With MNV group maintained a higher CVI than the control group in all 5 subfields(superior,temporal,central,inferior,and inferonasal;all P<0.05).In the central region,CONCLUSION:CVI decreases,and choroidal structural changes extend beyond the subfoveal area in cCSC with MNV eyes,providing with an imaging evidence for the important role of choroidal ischemia in the pathogenesis of MNV in cCSC.
基金supported by the National Natural Science Foundation of China(32171816)T4F program Sweden.
文摘Hybridization is a driving force in ecological transitions and speciation,yet direct evidence linking it to adaptive differentiation in natural systems remains limited.This study evaluates the role of hybridization in the speciation of Pinus densata,a keystone forest species on the southeastern Tibetan Plateau.By creating artificialinterspecificF1s and a long-term common garden experiment on the plateau,we provide in situ assessments on 44 growth and physiological traits across four seasons,along with RNA sequencing.We found significantphenotypic divergence between P.densata and its putative parental species P.tabuliformis and P.yunnanensis,with P.densata demonstrating superior growth and dynamic balance between photosynthesis and photoprotection.The F1s closely resembled P.densata in most traits.Gene expression revealed 19%–10%of 34,000 examined genes as differentially expressed in P.densata and F1s relative to mid-parent expression values.Both additive(4%)and non-additive gene actions(5%–6%in F1s,10%–12%in P.densata)were common,while transgressive expression occurred more frequently in the stabilized natural hybrids,illustrating transcriptomic reprogramming brought by hybridization and further divergence by natural selection.We provide compelling evidence for hybridization-derived phenotypic divergence at both physiological and gene expression levels that could have contributed to the adaptation of P.densata to high plateau habitat where both parental species have low fitness.The altered physiology and gene expression in hybrids serve both as a substrate for novel ecological adaptation and as a mechanism for the initiation of reproductive isolation.
