Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy worldwide.The development of new management strategi...Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy worldwide.The development of new management strategies becomes essential as effective chemical nematicides are progressively being restricted.Hence,we analysed grape pomace-derived biochars,pyrolysed at 350℃(BC350)and 700℃(BC700),focusing on their potential for RKN control.The thermal treatment of grape pomace caused an increase in the concentration of carbon and plant macro-and micronutrients,which were largely present in a water-soluble form.Synchrotron radiation-based Fourier transform infrared microspectroscopy data showed a general loss of carboxylic functional groups during pyrolysis,partially contributing to the alkalinisation of both biochars,mostly in BC700.Scanning electron microscopy coupled with energydispersive X-ray spectroscopy analysis revealed a highly porous structure filled with different crystals composed of elements such as K,Ca,Mg,P,Si or Al,which could be a suitable environment for the growth of microorganisms.Biochar-derived aqueous extracts showed phytotoxicity to tomato seedlings at high concentrations,and disappeared upon dilution,but no toxic effect was observed on the nematode’s infective stage.However,the infective and reproductive traits of a Meloidogyne javanica population in tomato were significantly reduced(i.e.egg masses and eggs per plant)in washed-biochar-treated soil in pots(0.75%;BC350W).Therefore,the large amount of grape waste generated after wine production can be transformed into a valuable product such as biochar,effective for RKNs control,thus reducing the waste management problem and contributing to a circular economy.展开更多
Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the...Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the use of synthetic nematicides, most of which have been banned due to their toxicity to humans and the environment. Farmer-participatory trials were established, for two consecutive growing seasons on farmers＇ fields infested with root-knot nematodes （A4eloidogvne javanica and Meloidogyne incognita）, at two sites in central Kenya （Maragua and Kirinyaga districts）, representing two contrasting group of farmers （low-input and high-input）. The trials aimed at evaluating the potential of using biological control agents （BCAs）, i.e. Poehonia ehlamydosporia and Pasteuria penetrans, antagonistic plants （Crotalaria grahamiana and Tagetes minuta）, ＂＇trash burning＂ and chemical control （dazomet 98% = Basamid~ Granular） in the management of root-knot nematodes in tomato nursery beds, and quantify the associated net benefits, compared to an untreated control. Crotalaria grahamiana, ＂trash burning＂ and dazomet, significantly （P 〈 0.05） reduced the number of second-stage juveniles （J2s） of the root-knot nematodes in the soil in nursery beds during the first season at Karigui-ini, but there was no significant （P 〉 0.05） increase in yield after transplanting tomato seedlings from the respective treatment. Similarly, one of the BCAs （P. ehlamydosporia）, was effectively transferred to the field with seedlings from the BCA-treated nursery beds, but there was no significant （P 〉 0.05） increase in yield due to slow proliferation of the BCA, after transplanting. Participatory partial budgets were successfully developed with srnallholder farmers, and proved to be a vital tool in making informed decisions on management options for root-knot nematodes. In general, labour was considered by all farmers as the major input that contributed to the overall additional cost of the different treatments at both sites. This necessitates improvement in efficiency of labour resource use.展开更多
The latest study published in Nature by Andrew R.Burns,Peter J.Roy and co-authors is highlighted in this paper,they investigated a series of novel nematicidal compounds,including Selectivin-A and Selectivin-E,and expl...The latest study published in Nature by Andrew R.Burns,Peter J.Roy and co-authors is highlighted in this paper,they investigated a series of novel nematicidal compounds,including Selectivin-A and Selectivin-E,and explored their mechanism of action.Experiments have displayed that the Selectivin compound is inactive to human cells,fish,fungi,insects and even beneficial nematodes.In the exploration of its mechanism of action,it was found that the mechanism of action of Selectivin is different with those of commercial nematocides:Selectivin needs to be activated by biotin produced by nematodes,after that they can be transformed into compounds with high nematicidal activity.This proves that the family of Selectivin compounds has the advantages of high selectivity and environmental friendliness,and their mechanism of action is completely new,proposing a completely new path for the development of new nematicides.展开更多
基金Spanish Government(PID2019-105924RB-I00 MCIN/AEI/10.13039/501100011033,RED2018-102407-T)to CE,and(AGL2014-53771-R,AGL2017-87591-R)to LEHCastilla-La Mancha Government(SBPLY/17/180501/000287 and SBPLY/21/180501/000033)to CE.+1 种基金AM-G was recipient of a PhD grant from Fundación Tatiana Pérez de Guzmán el BuenoEU FEDER funds complemented all the grants.
文摘Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy worldwide.The development of new management strategies becomes essential as effective chemical nematicides are progressively being restricted.Hence,we analysed grape pomace-derived biochars,pyrolysed at 350℃(BC350)and 700℃(BC700),focusing on their potential for RKN control.The thermal treatment of grape pomace caused an increase in the concentration of carbon and plant macro-and micronutrients,which were largely present in a water-soluble form.Synchrotron radiation-based Fourier transform infrared microspectroscopy data showed a general loss of carboxylic functional groups during pyrolysis,partially contributing to the alkalinisation of both biochars,mostly in BC700.Scanning electron microscopy coupled with energydispersive X-ray spectroscopy analysis revealed a highly porous structure filled with different crystals composed of elements such as K,Ca,Mg,P,Si or Al,which could be a suitable environment for the growth of microorganisms.Biochar-derived aqueous extracts showed phytotoxicity to tomato seedlings at high concentrations,and disappeared upon dilution,but no toxic effect was observed on the nematode’s infective stage.However,the infective and reproductive traits of a Meloidogyne javanica population in tomato were significantly reduced(i.e.egg masses and eggs per plant)in washed-biochar-treated soil in pots(0.75%;BC350W).Therefore,the large amount of grape waste generated after wine production can be transformed into a valuable product such as biochar,effective for RKNs control,thus reducing the waste management problem and contributing to a circular economy.
文摘Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the use of synthetic nematicides, most of which have been banned due to their toxicity to humans and the environment. Farmer-participatory trials were established, for two consecutive growing seasons on farmers＇ fields infested with root-knot nematodes （A4eloidogvne javanica and Meloidogyne incognita）, at two sites in central Kenya （Maragua and Kirinyaga districts）, representing two contrasting group of farmers （low-input and high-input）. The trials aimed at evaluating the potential of using biological control agents （BCAs）, i.e. Poehonia ehlamydosporia and Pasteuria penetrans, antagonistic plants （Crotalaria grahamiana and Tagetes minuta）, ＂＇trash burning＂ and chemical control （dazomet 98% = Basamid~ Granular） in the management of root-knot nematodes in tomato nursery beds, and quantify the associated net benefits, compared to an untreated control. Crotalaria grahamiana, ＂trash burning＂ and dazomet, significantly （P 〈 0.05） reduced the number of second-stage juveniles （J2s） of the root-knot nematodes in the soil in nursery beds during the first season at Karigui-ini, but there was no significant （P 〉 0.05） increase in yield after transplanting tomato seedlings from the respective treatment. Similarly, one of the BCAs （P. ehlamydosporia）, was effectively transferred to the field with seedlings from the BCA-treated nursery beds, but there was no significant （P 〉 0.05） increase in yield due to slow proliferation of the BCA, after transplanting. Participatory partial budgets were successfully developed with srnallholder farmers, and proved to be a vital tool in making informed decisions on management options for root-knot nematodes. In general, labour was considered by all farmers as the major input that contributed to the overall additional cost of the different treatments at both sites. This necessitates improvement in efficiency of labour resource use.
基金supported in part by the National Key Research and Development Program of China(2021YFD1700103)the National Natural Science Foundation of China(22177051,32061143045)+1 种基金the Fundamental Research Funds for the Central Universities(KYCYXT2022010)Sichuan Key Research and Development Program(22ZDYF0186,2021YFN0134).
文摘The latest study published in Nature by Andrew R.Burns,Peter J.Roy and co-authors is highlighted in this paper,they investigated a series of novel nematicidal compounds,including Selectivin-A and Selectivin-E,and explored their mechanism of action.Experiments have displayed that the Selectivin compound is inactive to human cells,fish,fungi,insects and even beneficial nematodes.In the exploration of its mechanism of action,it was found that the mechanism of action of Selectivin is different with those of commercial nematocides:Selectivin needs to be activated by biotin produced by nematodes,after that they can be transformed into compounds with high nematicidal activity.This proves that the family of Selectivin compounds has the advantages of high selectivity and environmental friendliness,and their mechanism of action is completely new,proposing a completely new path for the development of new nematicides.
