While illustrating the process and the essence of listening and analyzing various subjective and objective barriers in listening, the author tries to show that English learners can improve their quality of listening c...While illustrating the process and the essence of listening and analyzing various subjective and objective barriers in listening, the author tries to show that English learners can improve their quality of listening comprehension by transferring the negative elements to the positive ones.展开更多
The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5' flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) simil...The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5' flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp～ -2902bp, termed ε-NRAII) 5' to the cap site of this gene. NRF DNA fragment (-3010bp～ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression.展开更多
The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar ...The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp～ -2902bp, termed ε-NRAII) 5’ to the cap site of this gene. NRF DNA fragment (-3010bp～ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression.展开更多
Three design modes of seat suspension,i.e.,negative stiffness elements(NSEs),damping elements(DEs),and negative stiffness-damping elements(NSDEs),are proposed to evaluate the ride performance of a vehicle.Based on a d...Three design modes of seat suspension,i.e.,negative stiffness elements(NSEs),damping elements(DEs),and negative stiffness-damping elements(NSDEs),are proposed to evaluate the ride performance of a vehicle.Based on a dynamic model of a seat suspension and indexes of the root mean square deformation and acceleration of the seat suspension(x RMS)and driver s seat(a RMS),the influence of the design parameters of the NSEs,DEs,and NSDEs on the driver s ride comfort is evaluated.A genetic algorithm is then applied to optimize the parameters of the NSEs,DEs,and NSDEs.The study results indicate that the design parameters of the NSEs and NSDEs remarkably influence x RMS and a RMS,whereas those of the DEs insignificantly influence x RMS and a RMS.Based on the optimal results of the NSEs,DEs,and NSDEs,the damping force of the DEs is 98.3%lower than the restoring force of the NSEs.Therefore,the DEs are ineffective in decreasing x RMS and a RMS.Conversely,the NSEs combined with the damping coefficient of the seat suspension strongly reduce x RMS and a RMS.Consequently,the NSEs can be added to the seat suspension,and the damping coefficient of the seat suspension can also be optimized or controlled to further enhance the vehicle s ride performance.展开更多
文摘While illustrating the process and the essence of listening and analyzing various subjective and objective barriers in listening, the author tries to show that English learners can improve their quality of listening comprehension by transferring the negative elements to the positive ones.
基金This work was supported by National Natural Sciences Foun-dation of China, No. 39893320 and No. 39870378.
文摘The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5' flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp～ -2902bp, termed ε-NRAII) 5' to the cap site of this gene. NRF DNA fragment (-3010bp～ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression.
基金This work was supported by National Natural Sciences Foun-dation of China, No. 39893320 and No. 39870378.
文摘The developmental control of the human e-globin gene expression is mediated by transcription regulatory elements in the 5’ flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-кB consensus sequence resides in the negative regulatory element (-3028bp～ -2902bp, termed ε-NRAII) 5’ to the cap site of this gene. NRF DNA fragment (-3010bp～ -2986bp) containing the NF-кB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-кB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-кB motif) could not, suggesting that the binding protein is a member of NF-кB/Rel family. Western blot assay demonstrated that the molecular weight of NF-кB protein in the nuclei of K562 cells is 50ku. We suggested that NF-кB p50 may play an important role in the regulation of human c-globin gene expression.
基金The National Key Research and Development Plan(No.2019YFB2006402)the Talent Introduction Fund Project of Hubei Polytechnic University(No.19XJK20R)the Key Scientific Research Project of Hubei Polytechnic University(No.22xjz02A)。
文摘Three design modes of seat suspension,i.e.,negative stiffness elements(NSEs),damping elements(DEs),and negative stiffness-damping elements(NSDEs),are proposed to evaluate the ride performance of a vehicle.Based on a dynamic model of a seat suspension and indexes of the root mean square deformation and acceleration of the seat suspension(x RMS)and driver s seat(a RMS),the influence of the design parameters of the NSEs,DEs,and NSDEs on the driver s ride comfort is evaluated.A genetic algorithm is then applied to optimize the parameters of the NSEs,DEs,and NSDEs.The study results indicate that the design parameters of the NSEs and NSDEs remarkably influence x RMS and a RMS,whereas those of the DEs insignificantly influence x RMS and a RMS.Based on the optimal results of the NSEs,DEs,and NSDEs,the damping force of the DEs is 98.3%lower than the restoring force of the NSEs.Therefore,the DEs are ineffective in decreasing x RMS and a RMS.Conversely,the NSEs combined with the damping coefficient of the seat suspension strongly reduce x RMS and a RMS.Consequently,the NSEs can be added to the seat suspension,and the damping coefficient of the seat suspension can also be optimized or controlled to further enhance the vehicle s ride performance.
