【背景】东方蜜蜂微孢子虫(Nosema ceranae)专性侵染成年蜜蜂中肠上皮细胞而导致的微孢子虫病给养蜂业造成严重损失。【目的】检测东方蜜蜂微孢子虫nce-miR-23928及其靶基因在侵染意大利蜜蜂(Apis mellifera ligustica)工蜂过程的表达谱...【背景】东方蜜蜂微孢子虫(Nosema ceranae)专性侵染成年蜜蜂中肠上皮细胞而导致的微孢子虫病给养蜂业造成严重损失。【目的】检测东方蜜蜂微孢子虫nce-miR-23928及其靶基因在侵染意大利蜜蜂(Apis mellifera ligustica)工蜂过程的表达谱,为深入探究nce-miR-23928在东方蜜蜂微孢子虫侵染中的功能及调控机制提供依据。【方法】通过RNAhybrid、miRanda和TargetScan软件预测nce-miR-23928的靶基因。使用BLAST工具将上述靶基因比对到基因本体论(geneontology,GO)、京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)、Nr和Swiss-Prot数据库以获得相应注释。采用实时荧光定量PCR(real time quantitative PCR,RT-qPCR)技术检测nce-miR-23928及其靶基因在东方蜜蜂微孢子虫侵染意蜂工蜂过程中的相对表达量。【结果】相较于接种后1 d(1 day post infection,1 dpi),nce-miR-23928的表达量在2 dpi基本不变,而在4、6、8 dpi均为显著下调(P<0.05),总体表现出下降的表达趋势。预测到nce-miR-23928的15个靶基因,分别有9、4、15和9条靶基因可注释到GO(3个条目)、KEGG(7条通路)、Nr和Swiss-Prot数据库。与1 dpi相比,靶基因ABCT的表达量在2、4、6、8 dpi皆显著下调;靶基因SPTK的表达量在4、6、8 dpi均显著上调。【结论】明确nce-miR-23928及其靶基因ABCT和SPTK在东方蜜蜂微孢子虫侵染意蜂工蜂过程中的动态表达规律,揭示nce-miR-23928通过正调控ABCT表达和负调控SPTK表达可能在东方蜜蜂微孢子虫的侵染过程中起调节作用。展开更多
Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,...Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.展开更多
Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenou...Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenous educational quality, this paper concludes that the technological progress of information industry in China is skill- biased, i.e. technological progress of information industry increases the demand for skilled labor while decreases the demand for unskilled labor, and aggravates the wage difference between these two types of labor. Due to the effect of educational quality, the ratio of skilled labor employment to unskilled labor employment will reach a dynamic equilibrium and the wage difference between skilled labor and unskilled labor will not increase continuously. This paper then proves the foregoing conclusion with data of information industry in 26 provinces （autonomous region, municipality）. Focusing on typical skill-intensive information industry, this paper mainly studies the influence of educational quality, which not only complements the theoretical analysis on dual effects of technological progress on employment, but also provides feasible and practical basis to policies promoting the development of technological progress and full employment in China.展开更多
Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is t...Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is the response as a function of the correlation time of the dichotomous resistor.展开更多
文摘【背景】东方蜜蜂微孢子虫(Nosema ceranae)专性侵染成年蜜蜂中肠上皮细胞而导致的微孢子虫病给养蜂业造成严重损失。【目的】检测东方蜜蜂微孢子虫nce-miR-23928及其靶基因在侵染意大利蜜蜂(Apis mellifera ligustica)工蜂过程的表达谱,为深入探究nce-miR-23928在东方蜜蜂微孢子虫侵染中的功能及调控机制提供依据。【方法】通过RNAhybrid、miRanda和TargetScan软件预测nce-miR-23928的靶基因。使用BLAST工具将上述靶基因比对到基因本体论(geneontology,GO)、京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)、Nr和Swiss-Prot数据库以获得相应注释。采用实时荧光定量PCR(real time quantitative PCR,RT-qPCR)技术检测nce-miR-23928及其靶基因在东方蜜蜂微孢子虫侵染意蜂工蜂过程中的相对表达量。【结果】相较于接种后1 d(1 day post infection,1 dpi),nce-miR-23928的表达量在2 dpi基本不变,而在4、6、8 dpi均为显著下调(P<0.05),总体表现出下降的表达趋势。预测到nce-miR-23928的15个靶基因,分别有9、4、15和9条靶基因可注释到GO(3个条目)、KEGG(7条通路)、Nr和Swiss-Prot数据库。与1 dpi相比,靶基因ABCT的表达量在2、4、6、8 dpi皆显著下调;靶基因SPTK的表达量在4、6、8 dpi均显著上调。【结论】明确nce-miR-23928及其靶基因ABCT和SPTK在东方蜜蜂微孢子虫侵染意蜂工蜂过程中的动态表达规律,揭示nce-miR-23928通过正调控ABCT表达和负调控SPTK表达可能在东方蜜蜂微孢子虫的侵染过程中起调节作用。
基金supported by the Zhejiang Provincial Key Research and Development Plan(Grant No.2019C02006)the National Key Research and Development Program of China(Grant Nos.2016YFD0200804 and 2016YFD0100601-15)+2 种基金the Key Program of Zhejiang Provincial Foundation for Natural Science(Grant No.LZ16C130002)the Zhejiang Fundamental Public Welfare Research Program(Grant No.LGN19C140008)the State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products(Grant No.2010DS700124-ZZ1907).
文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.
基金This research is funded by the key project of National Social Science Foundation of China ＂Study on Major Issues and Countermeasures of Industrial Organization in China after the International Financial Crisis＂ （Approval No. 12AJY004） Major Research Projects on Humanities and Social Sciences of Shandong University ＂Study on Industrial Concentration, Enterprise Size and International Competitiveness of China＇s Manufacturing Industry against Economic Globalization＂ （Approval No.12RWZD15） Fund Project for Postgraduate＇s Independent Innovation of Shandong University ＂Study on the Impact Mechanism of IT Technical Progress on Employment.＂
文摘Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenous educational quality, this paper concludes that the technological progress of information industry in China is skill- biased, i.e. technological progress of information industry increases the demand for skilled labor while decreases the demand for unskilled labor, and aggravates the wage difference between these two types of labor. Due to the effect of educational quality, the ratio of skilled labor employment to unskilled labor employment will reach a dynamic equilibrium and the wage difference between skilled labor and unskilled labor will not increase continuously. This paper then proves the foregoing conclusion with data of information industry in 26 provinces （autonomous region, municipality）. Focusing on typical skill-intensive information industry, this paper mainly studies the influence of educational quality, which not only complements the theoretical analysis on dual effects of technological progress on employment, but also provides feasible and practical basis to policies promoting the development of technological progress and full employment in China.
基金The project supported by National Natural Science Foundation of China under Grant No, 10375009, and the Scientific Research Foundation for Research 0verseas Chinese Scholars of the State Education Ministry of China. and by K.C. Wong Magna Fund in Ningbo University
文摘Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is the response as a function of the correlation time of the dichotomous resistor.
