Objective: To explore the anti-nociceptive effect of patchouli alcohol(PA), the essential oil isolated from Pogostemon cablin(Blanco) Bent, and determine the mechanism in molecular levels. Methods: The acetic acid-ind...Objective: To explore the anti-nociceptive effect of patchouli alcohol(PA), the essential oil isolated from Pogostemon cablin(Blanco) Bent, and determine the mechanism in molecular levels. Methods: The acetic acid-induced writhing test and formalin-induced plantar injection test in mice were employed to con?rm the effect in vivo. Intracellular calcium ion was imaged to verify PA on mu-opioid receptor(MOR). Cyclooxygenase 2(COX2)and MOR of mouse brain were expressed for determination of PA’s target. Cellular experiments were carried out to find out COX2 and MOR expression induced by PA. Results: PA significantly reduced latency period of visceral pain and writhing induced by acetic acid saline solution(P<0.01) and allodynia after intra-plantar formalin(P<0.01) in mice. PA also up-regulated COX2 mRNA and protein(P<0.05) with a down-regulation of MOR(P<0.05) both in in vivo and in vitro experiments, which devote to the analgesic effect of PA. A decrease in the intracellular calcium level(P<0.05) induced by PA may play an important role in its anti-nociceptive effect.PA showed the characters of enhancing the MOR expression and reducing the intracellular calcium ion similar to opioid effect. Conclusions: Both COX2 and MOR are involved in the mechanism of PA’s anti-nociceptive effect,and the up-regulation of the receptor expression and the inhibition of intracellular calcium are a new perspective to PA’s effect on MOR.展开更多
To investigate lymphangiogenesis in tumor tissues and 5' Nase, Flt 4, Flk 1 expressions Methods 5' Nase and Aplase expressions and the morphology of lymphatics in gastric carcinoma tissues were observed...To investigate lymphangiogenesis in tumor tissues and 5' Nase, Flt 4, Flk 1 expressions Methods 5' Nase and Aplase expressions and the morphology of lymphatics in gastric carcinoma tissues were observed with enzyme histochemistry Flt 4 and Flk 1 expression in gastric carcinoma and sarcoma tissues was studied by immunohistochemistry CNE 2z cells were implanted into the subdermal layer of nude mice and hyaluronic acid was injected at the same sites After 4 or 5 weeks, nasopharyngeal carcinoma (NPC) xenograft tissues were cut for immunohistochemistry and enzyme histochemistry, and lymphangiogenesis, 5' Nase, Alpase, Flt 4 and Flk 1 expression in NPC tissues was studied Hyaluronic acid contents in sera of the patients with NPC and mice with NPC xenografts were measured by radioimmunoassay Results There were a lot of lymphatics and solid strip like tissues in gastric carcinoma and NPC xenograft treated with hyaluronic acid The total number of lymphatics (26 9±14 2/HP) increased significantly in gastric carcinoma tissues Flt 4 and Flk 1 positive expression was found in gastric carcinoma, sarcoma tissues and NPC xenograft Most of the vessels with positive Flt 4 were lymphatics Flk 1 expression was only found in the walls of blood vessels Hyaluronic acid content (363 52±98 15?ng/ml) in sera of patients with NPC was higher than that of the patients with chronic nasopharyngeal imflammation (306 29±27 15?ng/ml), with a significant difference ( P =0 039) The hyaluronic acid content in sera of nude mice with NPC (2120 59±490 22?ng/ml) was markedly higher than that in normal mice (1588 99±502 12?ng/ml), with a significant difference between them ( P =0 007) Conclusion There are a lot of lymph capillaries with positive 5' Nase in gastric carcinoma and NPC xenograft treated with hyaluronic acid Flt 4 positive expression is shown in gastric carcinoma, sarcoma tissues and NPC xenografts The results suggest there is lymphangiogenesis in these malignant tumor tissues Hyaluronic acid contents in sera of patients with NPC and nude mice with NPC increase, which may promote the formation of lymphatics展开更多
基金Supported by the National Natural Science Foundation of China(No.81374006 and No.81073092)the Special Foundation for New Drug Innovation of Tsinghua University(No.20142000077),China
文摘Objective: To explore the anti-nociceptive effect of patchouli alcohol(PA), the essential oil isolated from Pogostemon cablin(Blanco) Bent, and determine the mechanism in molecular levels. Methods: The acetic acid-induced writhing test and formalin-induced plantar injection test in mice were employed to con?rm the effect in vivo. Intracellular calcium ion was imaged to verify PA on mu-opioid receptor(MOR). Cyclooxygenase 2(COX2)and MOR of mouse brain were expressed for determination of PA’s target. Cellular experiments were carried out to find out COX2 and MOR expression induced by PA. Results: PA significantly reduced latency period of visceral pain and writhing induced by acetic acid saline solution(P<0.01) and allodynia after intra-plantar formalin(P<0.01) in mice. PA also up-regulated COX2 mRNA and protein(P<0.05) with a down-regulation of MOR(P<0.05) both in in vivo and in vitro experiments, which devote to the analgesic effect of PA. A decrease in the intracellular calcium level(P<0.05) induced by PA may play an important role in its anti-nociceptive effect.PA showed the characters of enhancing the MOR expression and reducing the intracellular calcium ion similar to opioid effect. Conclusions: Both COX2 and MOR are involved in the mechanism of PA’s anti-nociceptive effect,and the up-regulation of the receptor expression and the inhibition of intracellular calcium are a new perspective to PA’s effect on MOR.
基金hisstudywassupportedbygrantsfromtheBureauofPublicHealth Guangdong China !(No 1997337)
文摘To investigate lymphangiogenesis in tumor tissues and 5' Nase, Flt 4, Flk 1 expressions Methods 5' Nase and Aplase expressions and the morphology of lymphatics in gastric carcinoma tissues were observed with enzyme histochemistry Flt 4 and Flk 1 expression in gastric carcinoma and sarcoma tissues was studied by immunohistochemistry CNE 2z cells were implanted into the subdermal layer of nude mice and hyaluronic acid was injected at the same sites After 4 or 5 weeks, nasopharyngeal carcinoma (NPC) xenograft tissues were cut for immunohistochemistry and enzyme histochemistry, and lymphangiogenesis, 5' Nase, Alpase, Flt 4 and Flk 1 expression in NPC tissues was studied Hyaluronic acid contents in sera of the patients with NPC and mice with NPC xenografts were measured by radioimmunoassay Results There were a lot of lymphatics and solid strip like tissues in gastric carcinoma and NPC xenograft treated with hyaluronic acid The total number of lymphatics (26 9±14 2/HP) increased significantly in gastric carcinoma tissues Flt 4 and Flk 1 positive expression was found in gastric carcinoma, sarcoma tissues and NPC xenograft Most of the vessels with positive Flt 4 were lymphatics Flk 1 expression was only found in the walls of blood vessels Hyaluronic acid content (363 52±98 15?ng/ml) in sera of patients with NPC was higher than that of the patients with chronic nasopharyngeal imflammation (306 29±27 15?ng/ml), with a significant difference ( P =0 039) The hyaluronic acid content in sera of nude mice with NPC (2120 59±490 22?ng/ml) was markedly higher than that in normal mice (1588 99±502 12?ng/ml), with a significant difference between them ( P =0 007) Conclusion There are a lot of lymph capillaries with positive 5' Nase in gastric carcinoma and NPC xenograft treated with hyaluronic acid Flt 4 positive expression is shown in gastric carcinoma, sarcoma tissues and NPC xenografts The results suggest there is lymphangiogenesis in these malignant tumor tissues Hyaluronic acid contents in sera of patients with NPC and nude mice with NPC increase, which may promote the formation of lymphatics
