Efficient separation of ethylene(C_(2)H_(4))from ethane(C_(2)H_(6))via a one-step adsorption process is desirable yet challenging.In this work,we report a C_(2)H_(6)-selective polynuclear Tb-MOF[Tb_(9)(μ_(3)-O)_(2)(...Efficient separation of ethylene(C_(2)H_(4))from ethane(C_(2)H_(6))via a one-step adsorption process is desirable yet challenging.In this work,we report a C_(2)H_(6)-selective polynuclear Tb-MOF[Tb_(9)(μ_(3)-O)_(2)(μ_(3)-OH)_(12)(H_(2)O)_(9)(TCPE)_(3)]^(−)·[H_(3)O]^(+)·(solvents)_(x)(TCPE=tetrakis(4-carboxyphenyl)ethylene acid),NKU-200-Tb,assembled via the reticular chemistry principle.The resulting(4,12)-connected framework critically features a high density of nonpolar aromatic rings on the pore surface and forms a specific nanotrap for C_(2)H_(6)with multiple C-H…π interaction sites.As a result,NKU-200-Tb exhibits an inverse adsorption behavior with a high C_(2)H_(6)/C_(2)H_(4)selectivity of 2.06 and a large uptake ratio of 151%(60.27/39.95 cm^(3)g^(−1))at 298 K and 1 bar.The superior adsorption properties of NKU-200-Tb,combined with great structural stability,place it among the most promising stable C_(2)H_(6)-selective MOFs.Dynamic breakthrough experiments demonstrate that polymer-grade C_(2)H_(4)(>99.9%)can be harvested in one step from a binary mixture of C_(2)H_(6)/C_(2)H_(4)(10/90,v/v).This work signifies the synergy of pore surface chemistry and space confinement in promoting the challenging C_(2)H_(6)/C_(2)H_(4)separation.展开更多
Listeria monocytogenes is a human and veterinary pathogen,one of the most common agents of foodborne infections worldwide.It can cause severe complications such as meningitis or miscarriage.Antivirulence therapies,whi...Listeria monocytogenes is a human and veterinary pathogen,one of the most common agents of foodborne infections worldwide.It can cause severe complications such as meningitis or miscarriage.Antivirulence therapies,which target virulence factors such as pore-forming toxins,offer an alternative approach to combating infections.In this study,cholesterol-containing liposomal nanotraps effec-tively neutralized L.monocytogenes exotoxins,particularly listeriolysin O,thereby protecting mammalian cells.Notably,toxin neutraliza-tion was observed under both neutral and acidic conditions,where listeriolysin O activity is optimized to facilitate bacterial escape from the phagosome.Liposomal nanotraps were phagocytosed by macrophages and colocalized with intracellular Listeria,increasing the clearance rate of intracellular bacteria.These findings expand the potential use of broad-spectrum liposomal nanotrap therapy,which could be employed alongside current standard of care treatments to assist the immune system in controlling virulent pathogens.展开更多
The persistent need for sorbents capable of efficiently extracting selenium from radioactive wastewater has been a significant challenge.Herein,we introduce SCU-CPN-5,a robust and saleable adsorbent designed through p...The persistent need for sorbents capable of efficiently extracting selenium from radioactive wastewater has been a significant challenge.Herein,we introduce SCU-CPN-5,a robust and saleable adsorbent designed through principles of valence and configuration matching,which achieves remarkable removal of selenium oxo-anions from nuclear waste liquids.SCU-CPN-5 demonstrates unprecedented adsorption capacities,with 617 mg/g for SeO_(4)^(2-)and 333 mg/g for SeO_(3)^(2-).Additionally,it exhibits a removal efficiency of up to 99.7%for environmentally relevant concentrations of SeO_(4)^(2-).Notably,we employed in-situ Fouriertransform infrared spectroscopy to monitor the sorption process of selenium oxo-anions into the pores of the sorbent in real time,marking a significant advancement.This research not only introduces a novel approach to the design of functional materials for nuclear wastewater management but also provides a molecular-level understanding of the adsorption mechanism.展开更多
Circulating peptide is a potential source of biomarkers for cancer detection.However,the existence of large molecular weight proteins in plasma have a disastrous effect on circulating peptides isolating and detecting....Circulating peptide is a potential source of biomarkers for cancer detection.However,the existence of large molecular weight proteins in plasma have a disastrous effect on circulating peptides isolating and detecting.Herein,nanotrap fractionation following by mass spectrometry have been applied to quantify the levels of bradykinin (BK) and hydroxylated bradykinin (Hyp-BK) as a relative measure of KRAS-regulated prolyl-4-hydroxylase alpha-1 (P4HA1) which may serve as early diagnosis marker for pancreatic ductal adenocarcinoma (PDAC).We found that P4HA1 can be upregulated by KRASG12v,which is a PDAC driver mutation,using HPNE/KRAS and HPNE cells.And we revealed that P4HA1 is overexpressed in PDAC tumors,compared to normal and inflamed pancreatic tissues.RNA interference revealed that P4HA1 activity was primarily responsible for Hyp-BK production.Mass spectrometry analysis revealed that plasma Hyp-BK/BK ratio was higher in PDAC than pancreatitis patients and healthy controls,while the area under the receiver operating characteristic (ROC) curve (AUC) is 0.8209 (95%Cl,0.7269-0.9149).The Hyp-BK/BK association with PDAC was reproduced in another cohort,where this ratio was found to increase with advancing tumor stage.These novel findings paved the way for wider applications of Nanotrap coupled mass spectrometry as a powerful tool for revealing biosignatures from plasma.展开更多
Even the potential of T cell-mimicking nanotrap for long term viral control due to its overcoming of human immunodeficiency virus(HIV)genetic diversity and viral resistance,the robust HIV inhibition was not expected b...Even the potential of T cell-mimicking nanotrap for long term viral control due to its overcoming of human immunodeficiency virus(HIV)genetic diversity and viral resistance,the robust HIV inhibition was not expected because these nanotraps displayed no obvious advantages compared with the infinite host cells.Herein,a glycoprotein 120(gp120)-targeting polypeptide UM15 reinforced lymphocyte-mimicking nanotrap was constructed,and its improved HIV-1 inhibiting efficacy was validated.According to the results,the constructed nanotraps exhibited evident escaping ability from uptake of the mononuclear phagocyte system and highly improved binding ability with gp120 proteins.The constructed nanotraps neutralized all tested HIV-1 pseudo typed viruses with IC80 of 21.0μg/mL,and inhibited both X4-tropic and R5-tropic HIV-1 with IC80 of 34.4 and 20.6μg/mL,respectively.Approximately 40%of gp120 was observed to be shed from pseudo virus,and above 40%bystander T cells were prevented from gp120-induced death by the constructed nanotraps.The safety of the constructed nanotraps was confirmed both in vitro and in mice.Therefore,the constructed nanotraps could specifically neutralize free HIV-1,selectively bind with gp120 expressing HIV-1 infected cells,cause gp120 shedding,inhibit gp120-induced bystander T cell killing on the premise of safety,and were considered as promising therapeutic agents for precise inhibition of HIV.展开更多
基金supported by the National Natural Science Foundation of China(22171144 and 22001132)the Fundamental Research Funds for the Central Universities(Nankai University).
文摘Efficient separation of ethylene(C_(2)H_(4))from ethane(C_(2)H_(6))via a one-step adsorption process is desirable yet challenging.In this work,we report a C_(2)H_(6)-selective polynuclear Tb-MOF[Tb_(9)(μ_(3)-O)_(2)(μ_(3)-OH)_(12)(H_(2)O)_(9)(TCPE)_(3)]^(−)·[H_(3)O]^(+)·(solvents)_(x)(TCPE=tetrakis(4-carboxyphenyl)ethylene acid),NKU-200-Tb,assembled via the reticular chemistry principle.The resulting(4,12)-connected framework critically features a high density of nonpolar aromatic rings on the pore surface and forms a specific nanotrap for C_(2)H_(6)with multiple C-H…π interaction sites.As a result,NKU-200-Tb exhibits an inverse adsorption behavior with a high C_(2)H_(6)/C_(2)H_(4)selectivity of 2.06 and a large uptake ratio of 151%(60.27/39.95 cm^(3)g^(−1))at 298 K and 1 bar.The superior adsorption properties of NKU-200-Tb,combined with great structural stability,place it among the most promising stable C_(2)H_(6)-selective MOFs.Dynamic breakthrough experiments demonstrate that polymer-grade C_(2)H_(4)(>99.9%)can be harvested in one step from a binary mixture of C_(2)H_(6)/C_(2)H_(4)(10/90,v/v).This work signifies the synergy of pore surface chemistry and space confinement in promoting the challenging C_(2)H_(6)/C_(2)H_(4)separation.
基金supported by the Multidisciplinary Center for Infectious Diseases(MCID)(SA_02 to EB)the Swiss National Science Foundation(P500PB_222029 to HB).
文摘Listeria monocytogenes is a human and veterinary pathogen,one of the most common agents of foodborne infections worldwide.It can cause severe complications such as meningitis or miscarriage.Antivirulence therapies,which target virulence factors such as pore-forming toxins,offer an alternative approach to combating infections.In this study,cholesterol-containing liposomal nanotraps effec-tively neutralized L.monocytogenes exotoxins,particularly listeriolysin O,thereby protecting mammalian cells.Notably,toxin neutraliza-tion was observed under both neutral and acidic conditions,where listeriolysin O activity is optimized to facilitate bacterial escape from the phagosome.Liposomal nanotraps were phagocytosed by macrophages and colocalized with intracellular Listeria,increasing the clearance rate of intracellular bacteria.These findings expand the potential use of broad-spectrum liposomal nanotrap therapy,which could be employed alongside current standard of care treatments to assist the immune system in controlling virulent pathogens.
基金partially supported by the Intergovernmental International Cooperation of the National Key R&D Program of China(2022YFE0105300)the National Natural Science Foundation of China(22306136,22176139,U2267222,and U1967217)+1 种基金the New Cornerstone Science Foundation through the XPLORER PRIZEthe Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘The persistent need for sorbents capable of efficiently extracting selenium from radioactive wastewater has been a significant challenge.Herein,we introduce SCU-CPN-5,a robust and saleable adsorbent designed through principles of valence and configuration matching,which achieves remarkable removal of selenium oxo-anions from nuclear waste liquids.SCU-CPN-5 demonstrates unprecedented adsorption capacities,with 617 mg/g for SeO_(4)^(2-)and 333 mg/g for SeO_(3)^(2-).Additionally,it exhibits a removal efficiency of up to 99.7%for environmentally relevant concentrations of SeO_(4)^(2-).Notably,we employed in-situ Fouriertransform infrared spectroscopy to monitor the sorption process of selenium oxo-anions into the pores of the sorbent in real time,marking a significant advancement.This research not only introduces a novel approach to the design of functional materials for nuclear wastewater management but also provides a molecular-level understanding of the adsorption mechanism.
文摘Circulating peptide is a potential source of biomarkers for cancer detection.However,the existence of large molecular weight proteins in plasma have a disastrous effect on circulating peptides isolating and detecting.Herein,nanotrap fractionation following by mass spectrometry have been applied to quantify the levels of bradykinin (BK) and hydroxylated bradykinin (Hyp-BK) as a relative measure of KRAS-regulated prolyl-4-hydroxylase alpha-1 (P4HA1) which may serve as early diagnosis marker for pancreatic ductal adenocarcinoma (PDAC).We found that P4HA1 can be upregulated by KRASG12v,which is a PDAC driver mutation,using HPNE/KRAS and HPNE cells.And we revealed that P4HA1 is overexpressed in PDAC tumors,compared to normal and inflamed pancreatic tissues.RNA interference revealed that P4HA1 activity was primarily responsible for Hyp-BK production.Mass spectrometry analysis revealed that plasma Hyp-BK/BK ratio was higher in PDAC than pancreatitis patients and healthy controls,while the area under the receiver operating characteristic (ROC) curve (AUC) is 0.8209 (95%Cl,0.7269-0.9149).The Hyp-BK/BK association with PDAC was reproduced in another cohort,where this ratio was found to increase with advancing tumor stage.These novel findings paved the way for wider applications of Nanotrap coupled mass spectrometry as a powerful tool for revealing biosignatures from plasma.
基金The current work was supported by the National Natural Science Foundation of China(No.81502675).
文摘Even the potential of T cell-mimicking nanotrap for long term viral control due to its overcoming of human immunodeficiency virus(HIV)genetic diversity and viral resistance,the robust HIV inhibition was not expected because these nanotraps displayed no obvious advantages compared with the infinite host cells.Herein,a glycoprotein 120(gp120)-targeting polypeptide UM15 reinforced lymphocyte-mimicking nanotrap was constructed,and its improved HIV-1 inhibiting efficacy was validated.According to the results,the constructed nanotraps exhibited evident escaping ability from uptake of the mononuclear phagocyte system and highly improved binding ability with gp120 proteins.The constructed nanotraps neutralized all tested HIV-1 pseudo typed viruses with IC80 of 21.0μg/mL,and inhibited both X4-tropic and R5-tropic HIV-1 with IC80 of 34.4 and 20.6μg/mL,respectively.Approximately 40%of gp120 was observed to be shed from pseudo virus,and above 40%bystander T cells were prevented from gp120-induced death by the constructed nanotraps.The safety of the constructed nanotraps was confirmed both in vitro and in mice.Therefore,the constructed nanotraps could specifically neutralize free HIV-1,selectively bind with gp120 expressing HIV-1 infected cells,cause gp120 shedding,inhibit gp120-induced bystander T cell killing on the premise of safety,and were considered as promising therapeutic agents for precise inhibition of HIV.
