A traumatic tissue adhesive technology is highly sought after in ophthalmic surgery;however,many polymeric adhesives face significant limitations in clinical ophthalmology, particularly incorneal transplantation. A ma...A traumatic tissue adhesive technology is highly sought after in ophthalmic surgery;however,many polymeric adhesives face significant limitations in clinical ophthalmology, particularly incorneal transplantation. A major challenge is achieving rapid adhesion without introducingpolymer barriers or chemical toxicity from cross-linking. To address this, we developed a novelcornea-specific nanoadhesive constructed through protein-DNA co-assembly and applied it tocorneal transplantation. In this system, a rigid tetrahedral DNA framework was employed toguide the spatial distribution of polycationic recombinant proteins (K72) and serve as the coreof the nanoadhesive, facilitating energy conversion during tissue connection. The adhesivedemonstrated a strength of 2.3 kPa between corneal lenticules. After modification with RGDpeptides, the adhesive system significantly enhanced corneal epithelialization, reducedinflammation and neovascularization, and ultimately promoted corneal repair. This studyrepresents the first application of a nanoadhesive in ophthalmic surgery, providing a novelsolution for developing ophthalmic-specific adhesives for clinical use.展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.82471116,82271119,82371096,82401300,and 22277018)Shanghai RisingStar Program(23QA1401000)+3 种基金Healthy Young Talents Project of Shanghai Municipal Health Commission(2022YQ015)National Funded Postdoctoral Program of China(GZB20230158)China Postdoctoral Science Foundation(2024M750550)Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar(LR23B030001)。
文摘A traumatic tissue adhesive technology is highly sought after in ophthalmic surgery;however,many polymeric adhesives face significant limitations in clinical ophthalmology, particularly incorneal transplantation. A major challenge is achieving rapid adhesion without introducingpolymer barriers or chemical toxicity from cross-linking. To address this, we developed a novelcornea-specific nanoadhesive constructed through protein-DNA co-assembly and applied it tocorneal transplantation. In this system, a rigid tetrahedral DNA framework was employed toguide the spatial distribution of polycationic recombinant proteins (K72) and serve as the coreof the nanoadhesive, facilitating energy conversion during tissue connection. The adhesivedemonstrated a strength of 2.3 kPa between corneal lenticules. After modification with RGDpeptides, the adhesive system significantly enhanced corneal epithelialization, reducedinflammation and neovascularization, and ultimately promoted corneal repair. This studyrepresents the first application of a nanoadhesive in ophthalmic surgery, providing a novelsolution for developing ophthalmic-specific adhesives for clinical use.
