[Objectives]To study the effects of different application methods and application rates of biological nano-selenium on the quality of passion fruit.[Methods]In this experiment,passion fruit Tainong 1 was sprayed with ...[Objectives]To study the effects of different application methods and application rates of biological nano-selenium on the quality of passion fruit.[Methods]In this experiment,passion fruit Tainong 1 was sprayed with biological nano-selenium.The concentration of spraying refers to the recommended application rate of biological nano-selenium manufacturers.Three treatments with different concentrations were carried out:3,4.5 and 6 L/ha biological nano-selenium was sprayed on 100%root,(50%root+50%leaf surface)and 100%leaf surface of passion fruit,respectively.[Results]From the aspects of economic cost and rational utilization of resources,spraying 3 L/ha selenium fertilizer on the leaves of passion fruit played the best role in improving the quality of passion fruit.[Conclusions]To study the selenium-rich production technology of agricultural products and improve the quality of agricultural products is of great significance for improving human health and producing high-quality agricultural products.展开更多
AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV irradiation.METHODS:LB...AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV irradiation.METHODS:LBP-Se NPs were prepared and their particle size was detected.HLECs(SRA01/04)were irradiated with UVB for different time(0,10,20,30,40,50,60 min)to construct a damaged model,the survival rate of cells was determined by methylthiazol tetrazolium(MTT)assay.The 4’,6-Diamidine-2’-phenylindole dihydrochloride(DAPI)staining was used to observe the status of cell nucleus and drug entering cytoplasm through cell membrane in SRA01/04 cells after adding LBP-SENPS loaded with coumarin fluorescence agent 24 h under fluorescence microscope.SRA01/04 normal and UVB-damaged cells were treated with different amounts of LBP-Se NPs at different concentrations,cells proliferation were observed.RESULTS:The particle size of LBP-Se NPs was stable in the range of 150-200 nm.The survival rate changes with time after UVB irradiation were statistically significant.The 10 min of UVB exposure as the time was chosen to construct the cell damage model.With DAPI staining,LBPSe NPs were observed to enter the cytoplasm through the cell membrane under fluorescence inverted microscope.Cytotoxicity of SRA01/04 at different concentrations of LBPSe NPs were measured.Cell survival rate was statistically different compared with the control group.The higher the loading concentration of LBP in nano-Se drugs was,the higher the cell proliferation rate was(P<0.05).The lower the concentration of LBP-Se NPs,the higher the cell proliferation rate,showing a negative growth trend(P<0.05).The group with the highest average cell proliferation rate was 0.5μmol/L 2.0 mg/m L LBP-Se NPs(128.80%).When the 2.0 mg/m L LBP-Se NPs group was selected for cell photography,the cell density was higher at 0.5μmol/L.With the increase of concentration,SRA01/04 cells appeared more cytoplasm dehydration,cell shrinkage and apoptotic bodies,and cell density decreased.CONCLUSION:LBP-Se NPs has moderate particle size and good stability.LBP-Se NPs can protect HLECs(SRA01/04)from UVB-induced damage,and the cell proliferation rate is further increased with increasing the amount of loaded LBP and decreasing nano-selenium concentration.展开更多
[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of cr...[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of crops （tomato, leaf mustard, peanut, and carrot） were exogenous selenium fertilizer treated, to explore the selenium-enriching effects on these crops. [Results] Exogenous application of microbial nano-selenium can significantly increase the selenium content of the crops, of which the effects on big tomato （Weiyanuo） were the best. After spraying, the selenium content increased by 1.39 times, and at the same time the sugar content of tomato was also increased. [Conclusions] This study is expected to provide references for increase of the selenium content of agricultural products.展开更多
Cypermethrin (Cym) is a synthetic class II pyrethroid that is widely used and has a big risk to health. Cypermethrin produces oxidative stress and enhances inflammatory damage of liver. The present study was designed ...Cypermethrin (Cym) is a synthetic class II pyrethroid that is widely used and has a big risk to health. Cypermethrin produces oxidative stress and enhances inflammatory damage of liver. The present study was designed to investigate the ameliorating effects of NSe against Cym-induced hepatotoxicity in rats. For this purpose, twenty four male rats were divided into three groups. Group (I) was gavaged with Cym (control group), group (II) gavaged daily with Cym (1 mg/kg body weight), and group (III) gavaged with Cym + NSe (2.5 mg kg body weight/day, three times a week) for 21 days. Cypermethrin increased serum liver enzymes, oxidative stress and inflammatory markers. Administration of NSe significantly reduced the increased serum liver enzymes and inflammatory parameters and restored the antioxidant capacity in liver. Our results suggest that Nse exhibits promising hepato-protective effects against Cym-induced oxidative damage and inflammation.展开更多
基金Guangxi Science and Technology Plan Project(Gui Ke AA17202037,Gui Ke AD19245169,Gui Ke AD18281072)Yulin Science and Technology Project(Yu Shi Ke Neng 20194301,2019CXPT00A4).
文摘[Objectives]To study the effects of different application methods and application rates of biological nano-selenium on the quality of passion fruit.[Methods]In this experiment,passion fruit Tainong 1 was sprayed with biological nano-selenium.The concentration of spraying refers to the recommended application rate of biological nano-selenium manufacturers.Three treatments with different concentrations were carried out:3,4.5 and 6 L/ha biological nano-selenium was sprayed on 100%root,(50%root+50%leaf surface)and 100%leaf surface of passion fruit,respectively.[Results]From the aspects of economic cost and rational utilization of resources,spraying 3 L/ha selenium fertilizer on the leaves of passion fruit played the best role in improving the quality of passion fruit.[Conclusions]To study the selenium-rich production technology of agricultural products and improve the quality of agricultural products is of great significance for improving human health and producing high-quality agricultural products.
基金Supported by the National Natural Science Foundation of China(No.81970806)Medical Scientific Research Foundation of Guangdong Province of China(No.A2019098)。
文摘AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV irradiation.METHODS:LBP-Se NPs were prepared and their particle size was detected.HLECs(SRA01/04)were irradiated with UVB for different time(0,10,20,30,40,50,60 min)to construct a damaged model,the survival rate of cells was determined by methylthiazol tetrazolium(MTT)assay.The 4’,6-Diamidine-2’-phenylindole dihydrochloride(DAPI)staining was used to observe the status of cell nucleus and drug entering cytoplasm through cell membrane in SRA01/04 cells after adding LBP-SENPS loaded with coumarin fluorescence agent 24 h under fluorescence microscope.SRA01/04 normal and UVB-damaged cells were treated with different amounts of LBP-Se NPs at different concentrations,cells proliferation were observed.RESULTS:The particle size of LBP-Se NPs was stable in the range of 150-200 nm.The survival rate changes with time after UVB irradiation were statistically significant.The 10 min of UVB exposure as the time was chosen to construct the cell damage model.With DAPI staining,LBPSe NPs were observed to enter the cytoplasm through the cell membrane under fluorescence inverted microscope.Cytotoxicity of SRA01/04 at different concentrations of LBPSe NPs were measured.Cell survival rate was statistically different compared with the control group.The higher the loading concentration of LBP in nano-Se drugs was,the higher the cell proliferation rate was(P<0.05).The lower the concentration of LBP-Se NPs,the higher the cell proliferation rate,showing a negative growth trend(P<0.05).The group with the highest average cell proliferation rate was 0.5μmol/L 2.0 mg/m L LBP-Se NPs(128.80%).When the 2.0 mg/m L LBP-Se NPs group was selected for cell photography,the cell density was higher at 0.5μmol/L.With the increase of concentration,SRA01/04 cells appeared more cytoplasm dehydration,cell shrinkage and apoptotic bodies,and cell density decreased.CONCLUSION:LBP-Se NPs has moderate particle size and good stability.LBP-Se NPs can protect HLECs(SRA01/04)from UVB-induced damage,and the cell proliferation rate is further increased with increasing the amount of loaded LBP and decreasing nano-selenium concentration.
基金Supported by Program of Local Specialty Crop Varieties and Protective Cultivation Technique in Foshan City(Agricultural Technology Extension Project of Foshan City in 2017-2018)Characteristic Vegetable Innovation Team of Modern Agricultural Industrial Technology System of Guangdong Province(2016LM1015)
文摘[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of crops （tomato, leaf mustard, peanut, and carrot） were exogenous selenium fertilizer treated, to explore the selenium-enriching effects on these crops. [Results] Exogenous application of microbial nano-selenium can significantly increase the selenium content of the crops, of which the effects on big tomato （Weiyanuo） were the best. After spraying, the selenium content increased by 1.39 times, and at the same time the sugar content of tomato was also increased. [Conclusions] This study is expected to provide references for increase of the selenium content of agricultural products.
文摘Cypermethrin (Cym) is a synthetic class II pyrethroid that is widely used and has a big risk to health. Cypermethrin produces oxidative stress and enhances inflammatory damage of liver. The present study was designed to investigate the ameliorating effects of NSe against Cym-induced hepatotoxicity in rats. For this purpose, twenty four male rats were divided into three groups. Group (I) was gavaged with Cym (control group), group (II) gavaged daily with Cym (1 mg/kg body weight), and group (III) gavaged with Cym + NSe (2.5 mg kg body weight/day, three times a week) for 21 days. Cypermethrin increased serum liver enzymes, oxidative stress and inflammatory markers. Administration of NSe significantly reduced the increased serum liver enzymes and inflammatory parameters and restored the antioxidant capacity in liver. Our results suggest that Nse exhibits promising hepato-protective effects against Cym-induced oxidative damage and inflammation.
