Multinuclear 3d-4f nano-clusters,consisting of a large number of metal ions,are interesting both structurally and functionally.The Gd(Ⅲ)containing clusters,in particular,attract great research attention because of th...Multinuclear 3d-4f nano-clusters,consisting of a large number of metal ions,are interesting both structurally and functionally.The Gd(Ⅲ)containing clusters,in particular,attract great research attention because of their significant magnetocaloric effect.Here,we report the synthesis of a gigantic 3d-4f wheel-like cluster.展开更多
The synthesis of gold nanoclusters within protein structures,such as TetX2 monooxygenase,presents a promising approach for obtaining nanostructures with enzyme-like activity.This is attributed to the presence of vario...The synthesis of gold nanoclusters within protein structures,such as TetX2 monooxygenase,presents a promising approach for obtaining nanostructures with enzyme-like activity.This is attributed to the presence of various amino acid groups that serve as biological templates for the formation and reduction of gold.The low toxicity,biocompatibility,solubility in aqueous systems,and specific fluorescence spectrum of these nanostructures enhance their utility in the detection of various analytes.The research involved expressing and purifying the recombinant enzyme TetX2,synthesizing gold on the TetX2 substrate,and investigating the effect of nanoclusters on the activity and structure of the TetX2 enzyme.The synthesis of gold nanoclusters(AuNCs)was carried out within the structure of TetX2.Finally,the accuracy of the AuNCs synthesis was verified by examining the morphology,size,spectroscopy,and catalytic activity.Results showed changes in the tertiary structure of the TetX2 enzyme in the presence of HAuCl4,loss of enzyme activity after the formation of gold nanoclusters,and successful incorporation of gold into the structure of TetX2.TetX2@AuNCs emitted blue light at 450 nm,and their catalytic properties were demonstrated through color changes in a chromogenic substrate(3,3′,5,5′-Tetramethylbenzidine-H2O2),indicating their authenticity.TetX2 is proposed as a new bio-template forthe synthesis of gold nanoclusters,with potential applications in biosensor design(Graphical abstract).展开更多
基金supported by the NSF of China(21871262 and 21901242)the NSF of Fujian Province(2020J05080),the NSF of Xiamen(3502Z20206080)+3 种基金the Key Research Program of the Chinese Academy of Sciences(ZDRW-CN-2021-3-3)the Youth Innovation Promotion Association CAS(2021302)the Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China(2021ZR110)the Recruitment Program of Global Youth Experts.
文摘Multinuclear 3d-4f nano-clusters,consisting of a large number of metal ions,are interesting both structurally and functionally.The Gd(Ⅲ)containing clusters,in particular,attract great research attention because of their significant magnetocaloric effect.Here,we report the synthesis of a gigantic 3d-4f wheel-like cluster.
基金financially supported by Nora Gene Pishro Companythe support from the University of Tehran.
文摘The synthesis of gold nanoclusters within protein structures,such as TetX2 monooxygenase,presents a promising approach for obtaining nanostructures with enzyme-like activity.This is attributed to the presence of various amino acid groups that serve as biological templates for the formation and reduction of gold.The low toxicity,biocompatibility,solubility in aqueous systems,and specific fluorescence spectrum of these nanostructures enhance their utility in the detection of various analytes.The research involved expressing and purifying the recombinant enzyme TetX2,synthesizing gold on the TetX2 substrate,and investigating the effect of nanoclusters on the activity and structure of the TetX2 enzyme.The synthesis of gold nanoclusters(AuNCs)was carried out within the structure of TetX2.Finally,the accuracy of the AuNCs synthesis was verified by examining the morphology,size,spectroscopy,and catalytic activity.Results showed changes in the tertiary structure of the TetX2 enzyme in the presence of HAuCl4,loss of enzyme activity after the formation of gold nanoclusters,and successful incorporation of gold into the structure of TetX2.TetX2@AuNCs emitted blue light at 450 nm,and their catalytic properties were demonstrated through color changes in a chromogenic substrate(3,3′,5,5′-Tetramethylbenzidine-H2O2),indicating their authenticity.TetX2 is proposed as a new bio-template forthe synthesis of gold nanoclusters,with potential applications in biosensor design(Graphical abstract).
