This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 ne...This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.展开更多
Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered secur...Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered security approach is often insufficient for safeguarding sensitive data.This study proposes a triple-lightweight cryptographic and steganographic model that integrates the Hill Cipher Technique(HCT),Rotation Left Digits(RLD),and Discrete Wavelet Transform(DWT)to embed secret messages within video frames securely.The approach begins with encrypting the secret text using a private key matrix(PK^(1))of size 2×2 up to 6×6 via HCT.A second encryption layer is applied using a dynamic private key(PK2)derived from the RGB pixel values of the video frame,resulting in a rotated cipher.The doubly encrypted message is then embedded into the video frames using the DWT method.Upon transmission,the concealed message is extracted using inverse DWT and decrypted in two steps—first with PK2 and then with the inverse of PK^(1).Experiments conducted using MPEG video sequences and message lengths ranging from 10 to 300 bytes demonstrate strong performance in terms of Mean Square Error(MSE),Peak Signal-to-Noise Ratio(PSNR),and Correlation Coefficient(CC)between original and encrypted messages.The similarity between original and stego frames is further validated using Structural Similarity Index(SSIM),Mean Absolute Error(MAE),Number of Pixel Change Rate(NPCR),and Unified Average Changing Intensity(UACI).Results confirm that utilizing video frames to generate PK2 offers superior security compared to static key images.Moreover,the indistinguishability between original and stego frames highlights the method’s robustness against visual and statistical attacks.展开更多
目的:基于巢式聚合酶链式反应(PCR)理念设计并筛选出可用于高效扩增与鉴别紫草市场样品真伪的特异性引物。方法:针对新疆紫草的ITS序列和紫草非《中华人民共和国药典》品ITS2序列,利用Primer Premier 5软件进行巢式引物设计;比较ITS2通...目的:基于巢式聚合酶链式反应(PCR)理念设计并筛选出可用于高效扩增与鉴别紫草市场样品真伪的特异性引物。方法:针对新疆紫草的ITS序列和紫草非《中华人民共和国药典》品ITS2序列,利用Primer Premier 5软件进行巢式引物设计;比较ITS2通用引物PCR和巢式PCR对紫草药材基因组DNA的扩增效率;基于巢式引物直接扩增紫草基因组DNA,并进行琼脂糖凝胶电泳检测;基于扩增产物片段长度、变异位点覆盖情况对设计的紫草药材特异性引物进行评价。结果:经过Primer Premier 5软件进行引物设计共选出11对引物进行合成;巢式PCR对紫草药材基因组DNA的扩增效率明显优于ITS2通用引物PCR;基于巢式引物直接扩增紫草基因组DNA琼脂糖凝胶电泳检测的结果明显优于ITS2引物直接扩增紫草基因组DNA,且呈单一条带;确定AE-9S/AE-2A、AE-4S/AE-10A、AE-12S/10A、AE-29S/AE-29A共4对引物适用于紫草正伪品鉴定。结论:在DNA条形码鉴定和巢式PCR技术的基础上,确定了4对可以用于有效区分药材市场中主流的新疆紫草和紫草非《中华人民共和国药典》品的特异性引物,为后续紫草药材及其他中药民族药品种的鉴定方法研究与开发提供了可参考的依据。展开更多
文摘This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.
文摘Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered security approach is often insufficient for safeguarding sensitive data.This study proposes a triple-lightweight cryptographic and steganographic model that integrates the Hill Cipher Technique(HCT),Rotation Left Digits(RLD),and Discrete Wavelet Transform(DWT)to embed secret messages within video frames securely.The approach begins with encrypting the secret text using a private key matrix(PK^(1))of size 2×2 up to 6×6 via HCT.A second encryption layer is applied using a dynamic private key(PK2)derived from the RGB pixel values of the video frame,resulting in a rotated cipher.The doubly encrypted message is then embedded into the video frames using the DWT method.Upon transmission,the concealed message is extracted using inverse DWT and decrypted in two steps—first with PK2 and then with the inverse of PK^(1).Experiments conducted using MPEG video sequences and message lengths ranging from 10 to 300 bytes demonstrate strong performance in terms of Mean Square Error(MSE),Peak Signal-to-Noise Ratio(PSNR),and Correlation Coefficient(CC)between original and encrypted messages.The similarity between original and stego frames is further validated using Structural Similarity Index(SSIM),Mean Absolute Error(MAE),Number of Pixel Change Rate(NPCR),and Unified Average Changing Intensity(UACI).Results confirm that utilizing video frames to generate PK2 offers superior security compared to static key images.Moreover,the indistinguishability between original and stego frames highlights the method’s robustness against visual and statistical attacks.
文摘目的:基于巢式聚合酶链式反应(PCR)理念设计并筛选出可用于高效扩增与鉴别紫草市场样品真伪的特异性引物。方法:针对新疆紫草的ITS序列和紫草非《中华人民共和国药典》品ITS2序列,利用Primer Premier 5软件进行巢式引物设计;比较ITS2通用引物PCR和巢式PCR对紫草药材基因组DNA的扩增效率;基于巢式引物直接扩增紫草基因组DNA,并进行琼脂糖凝胶电泳检测;基于扩增产物片段长度、变异位点覆盖情况对设计的紫草药材特异性引物进行评价。结果:经过Primer Premier 5软件进行引物设计共选出11对引物进行合成;巢式PCR对紫草药材基因组DNA的扩增效率明显优于ITS2通用引物PCR;基于巢式引物直接扩增紫草基因组DNA琼脂糖凝胶电泳检测的结果明显优于ITS2引物直接扩增紫草基因组DNA,且呈单一条带;确定AE-9S/AE-2A、AE-4S/AE-10A、AE-12S/10A、AE-29S/AE-29A共4对引物适用于紫草正伪品鉴定。结论:在DNA条形码鉴定和巢式PCR技术的基础上,确定了4对可以用于有效区分药材市场中主流的新疆紫草和紫草非《中华人民共和国药典》品的特异性引物,为后续紫草药材及其他中药民族药品种的鉴定方法研究与开发提供了可参考的依据。
