Short-time critical behavior of the random n-vector model is studied by the theoretic renormalization-group approach.Asymptotic scaling laws are studied in a frame of the expansion in e = 4 - d for n ≠ 1 and for n = ...Short-time critical behavior of the random n-vector model is studied by the theoretic renormalization-group approach.Asymptotic scaling laws are studied in a frame of the expansion in e = 4 - d for n ≠ 1 and for n = 1respectively.In d ＜ 4,the initial slip exponents θ′ for the order parameter and θ for the response function are calculated up to the second order in e ＝ 4 - d for n ≠ 1 and for n ＝ 1 at the random fixed point respectively.Our results show that the random impurities exert a strong influence on the short-time dynamics for d ＜ 4 and n ＜ nc.展开更多
目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌...目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌高转移细胞株mHCC-97中进行表达,检测稳定转染前后m asp in表达的变化。结果重组质粒在大肠杆菌株JM109内扩增。提纯、纯化后用EcoRⅠ、XbaⅠ酶切鉴定及测序鉴定证明m asp in基因已完整、正确的插入到pEFIRES-N表达载体,并在肝癌MHCC-97细胞中上调了m asp in基因mRNA和蛋白水平表达,抑制了肝癌MHCC-97细胞增殖及浸润。结论成功构建了m asp in/pEFIRES-N表达载体,能在真核细胞中表达。展开更多
文摘Short-time critical behavior of the random n-vector model is studied by the theoretic renormalization-group approach.Asymptotic scaling laws are studied in a frame of the expansion in e = 4 - d for n ≠ 1 and for n = 1respectively.In d ＜ 4,the initial slip exponents θ′ for the order parameter and θ for the response function are calculated up to the second order in e ＝ 4 - d for n ≠ 1 and for n ＝ 1 at the random fixed point respectively.Our results show that the random impurities exert a strong influence on the short-time dynamics for d ＜ 4 and n ＜ nc.
文摘目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌高转移细胞株mHCC-97中进行表达,检测稳定转染前后m asp in表达的变化。结果重组质粒在大肠杆菌株JM109内扩增。提纯、纯化后用EcoRⅠ、XbaⅠ酶切鉴定及测序鉴定证明m asp in基因已完整、正确的插入到pEFIRES-N表达载体,并在肝癌MHCC-97细胞中上调了m asp in基因mRNA和蛋白水平表达,抑制了肝癌MHCC-97细胞增殖及浸润。结论成功构建了m asp in/pEFIRES-N表达载体,能在真核细胞中表达。
