CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts....CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts. Matrix clusters and adducts interfere with peptide ionization and peptide mass spectrum interpretation, especially at low sample concentrations and within m/z 500-1 000 range. These matrix adducts, especially within m/z 500-1 000, are significantly reduced with addition of ammonium citrate, ammonium sulfate, ammonium benzoate, ammonium bicarbonate, ammonium citrate and potassium dihydrogen phosphate to the matrix and the samples were deposited onto the MALDI target. The reduction of matrix adducts improves peptide ionization and signal-to-noise ratio, which enhances the reliability of database searching.展开更多
Parkinson’s disease is a common chronic neurodegenerative disease.N-methylation can enhance the neurotoxiicity to dopaminergic neuron in the metabolic process of catechol isoquinolines and Salsolinol N-methyltransfer...Parkinson’s disease is a common chronic neurodegenerative disease.N-methylation can enhance the neurotoxiicity to dopaminergic neuron in the metabolic process of catechol isoquinolines and Salsolinol N-methyltransferases (SNMT) may play an important role in the pathogenesis of PD.A new method including SNMT purification and detecting SNMT activity was developed and displays an excellent sensitivity and stability.In addition,the SNMT activity in Wistar rats substantia nigra,striatum and cerebellum.展开更多
文摘CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts. Matrix clusters and adducts interfere with peptide ionization and peptide mass spectrum interpretation, especially at low sample concentrations and within m/z 500-1 000 range. These matrix adducts, especially within m/z 500-1 000, are significantly reduced with addition of ammonium citrate, ammonium sulfate, ammonium benzoate, ammonium bicarbonate, ammonium citrate and potassium dihydrogen phosphate to the matrix and the samples were deposited onto the MALDI target. The reduction of matrix adducts improves peptide ionization and signal-to-noise ratio, which enhances the reliability of database searching.
文摘Parkinson’s disease is a common chronic neurodegenerative disease.N-methylation can enhance the neurotoxiicity to dopaminergic neuron in the metabolic process of catechol isoquinolines and Salsolinol N-methyltransferases (SNMT) may play an important role in the pathogenesis of PD.A new method including SNMT purification and detecting SNMT activity was developed and displays an excellent sensitivity and stability.In addition,the SNMT activity in Wistar rats substantia nigra,striatum and cerebellum.
