AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitonea...AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitoneally 1 h prior to the first injection of cerulein.Once AP developed,the stable cholecystokinin analogue,cerulein was injected hourly,over a 6 h period.Blood samples were taken 6 h later to determine serum amylase,lipase,and cytokine levels.The pancreas and lungs were rapidly removed for morphological examination,myeloperoxidase assay,and real-time reverse transcription polymerase chain reaction.To specify the role of SSM in pancreatitis,the pancreatic acinar cells were isolated using collagenase method.Then the cells were pre-treated with SSM,then stimulated with cerulein.The cell viability,cytokine productions and high-mobility group box protein-1(HMGB-1) were measured.Furthermore,the regulating mechanisms of SSM action were evaluated.RESULTS:The administration of SSM significantly attenuated the severity of pancreatitis and pancreatitis associated lung injury,as was shown by the reduction in pancreatic edema,neutrophil infiltration,vacuolization and necrosis.SSM treatment also reduced pancreatic weight/body weight ratio,serum amylase,lipase and cytokine levels,and mRNA expression of multiple inflammatory mediators such as tumor necrosis factor-α and interleukin-1β.In addition,treatment with SSM inhibited HMGB-1 expression in the pancreas during AP.In accordance with in vivo data,SSM inhibited the cerulein-induced acinar cell death,cytokine,and HMGB-1 release.SSM also inhibited the activation of c-Jun NH2-terminal kinase,p38 and nuclear factor(NF)-κB.CONCLUSION:These results suggest that SSM plays a protective role during the development of AP and pancreatitis associated lung injury via deactivating c-Jun NH2-terminal kinase,p38 and NF-κB.展开更多
Arthropoda,a diverse phylum encompassing Myriapoda,Crustacea,Chelicerata,and Insecta,constitutes more than 80%of the total number of documented species(Qu et al.2020).Within these groups,centipedes(Chilopoda)hold a un...Arthropoda,a diverse phylum encompassing Myriapoda,Crustacea,Chelicerata,and Insecta,constitutes more than 80%of the total number of documented species(Qu et al.2020).Within these groups,centipedes(Chilopoda)hold a unique position as one of the oldest terrestrial venomous groups,with a fossil history spanning 430 million years.This lineage encompasses five orders and includes more than 3500 species(Undheim&King 2011).Centipedes,characterized by having a pair of legs per segment,have drawn significant attention in the biomedical field(Undheim et al.2015)due to their production of venoms in specialized venom glands that have modified the first pair of trunk legs(Giribet&Edgecombe 2019).Centipedes are carnivorous,soil-dwelling invertebrates with a predatory nature,playing a pivotal role as indicators of soil biodiversity and serving as vital tools for evaluating ecosystem health(Dugon 2017;Halpin et al.2021).Despite their ecological value,the evolutionary ecology of myriapods has received relatively less attention compared to other arthropods.However,recent advances have been made,with nonchromosomallevel genomes of five millipedes and four centipedes having been published,shedding more light on their evolutionary history(Chipman et al.2014;Kenny et al.2015;Qu et al.2020;So et al.2022).展开更多
Objective: To study the quinoline alkaloids from the ethanol extract of Scolopendra subspinipes mutilans(SSM).Methods: The chemical constituents were isolated and purified by macroporous resin column, medium pressure ...Objective: To study the quinoline alkaloids from the ethanol extract of Scolopendra subspinipes mutilans(SSM).Methods: The chemical constituents were isolated and purified by macroporous resin column, medium pressure preparation chromatography, and semi-preparative HPLC. Their structures were elucidated by IR,MS, and NMR experiments.Results: Three quinolone alkaloids were obtained and identified as 3-hydroxy-4-methoxyquinolin-8-yl hydrogen sulfate(1), jineol-8-sulfate(2), and jineol(3), respectively.Conclusion: Compound 1 is a new compound from SSM.展开更多
基金Supported by National Research Foundation of Korea grant funded by the Korea government MEST,No. 2010-0029498
文摘AIM:To evaluate the inhibitory effects of Scolopendra subspinipes mutilans(SSM) on cerulein-induced acute pancreatitis(AP) in a mouse model.METHODS:SSM water extract(0.1,0.5,or 1 g/kg) was administrated intraperitoneally 1 h prior to the first injection of cerulein.Once AP developed,the stable cholecystokinin analogue,cerulein was injected hourly,over a 6 h period.Blood samples were taken 6 h later to determine serum amylase,lipase,and cytokine levels.The pancreas and lungs were rapidly removed for morphological examination,myeloperoxidase assay,and real-time reverse transcription polymerase chain reaction.To specify the role of SSM in pancreatitis,the pancreatic acinar cells were isolated using collagenase method.Then the cells were pre-treated with SSM,then stimulated with cerulein.The cell viability,cytokine productions and high-mobility group box protein-1(HMGB-1) were measured.Furthermore,the regulating mechanisms of SSM action were evaluated.RESULTS:The administration of SSM significantly attenuated the severity of pancreatitis and pancreatitis associated lung injury,as was shown by the reduction in pancreatic edema,neutrophil infiltration,vacuolization and necrosis.SSM treatment also reduced pancreatic weight/body weight ratio,serum amylase,lipase and cytokine levels,and mRNA expression of multiple inflammatory mediators such as tumor necrosis factor-α and interleukin-1β.In addition,treatment with SSM inhibited HMGB-1 expression in the pancreas during AP.In accordance with in vivo data,SSM inhibited the cerulein-induced acinar cell death,cytokine,and HMGB-1 release.SSM also inhibited the activation of c-Jun NH2-terminal kinase,p38 and nuclear factor(NF)-κB.CONCLUSION:These results suggest that SSM plays a protective role during the development of AP and pancreatitis associated lung injury via deactivating c-Jun NH2-terminal kinase,p38 and NF-κB.
基金supported by the Hubei Provincial Natural Science Foundation and Innovative Development of Chinese Medicine—of China(2023AFD144)the Funds for Distinguished Young Scholars of Hubei University of Chinese Medicine(2022ZZXJ1003)to L.Z.
文摘Arthropoda,a diverse phylum encompassing Myriapoda,Crustacea,Chelicerata,and Insecta,constitutes more than 80%of the total number of documented species(Qu et al.2020).Within these groups,centipedes(Chilopoda)hold a unique position as one of the oldest terrestrial venomous groups,with a fossil history spanning 430 million years.This lineage encompasses five orders and includes more than 3500 species(Undheim&King 2011).Centipedes,characterized by having a pair of legs per segment,have drawn significant attention in the biomedical field(Undheim et al.2015)due to their production of venoms in specialized venom glands that have modified the first pair of trunk legs(Giribet&Edgecombe 2019).Centipedes are carnivorous,soil-dwelling invertebrates with a predatory nature,playing a pivotal role as indicators of soil biodiversity and serving as vital tools for evaluating ecosystem health(Dugon 2017;Halpin et al.2021).Despite their ecological value,the evolutionary ecology of myriapods has received relatively less attention compared to other arthropods.However,recent advances have been made,with nonchromosomallevel genomes of five millipedes and four centipedes having been published,shedding more light on their evolutionary history(Chipman et al.2014;Kenny et al.2015;Qu et al.2020;So et al.2022).
基金supported by the Special Project (No. 1811) for the Scientific & Technological Innovation and Development of Lanshan District, Linyi
文摘Objective: To study the quinoline alkaloids from the ethanol extract of Scolopendra subspinipes mutilans(SSM).Methods: The chemical constituents were isolated and purified by macroporous resin column, medium pressure preparation chromatography, and semi-preparative HPLC. Their structures were elucidated by IR,MS, and NMR experiments.Results: Three quinolone alkaloids were obtained and identified as 3-hydroxy-4-methoxyquinolin-8-yl hydrogen sulfate(1), jineol-8-sulfate(2), and jineol(3), respectively.Conclusion: Compound 1 is a new compound from SSM.
