Proteorhodopsin （PR） is a recently discovered protein involved in the utilization of light energy. Several studies have shown that PR-containing microorganisms are widespread and compose a large proportion of the bi...Proteorhodopsin （PR） is a recently discovered protein involved in the utilization of light energy. Several studies have shown that PR-containing microorganisms are widespread and compose a large proportion of the biomass in marine ecosystems. A better understanding of the ecological role of PR will help clarify the effect of the global flow of energy and the carbon cycle on marine communities. In this study, a bioinformatical database of PR codon sequences, the Global Distribution Database of Proteorhodopsin （GDDP）, as a tool for analyzing the diversity and distribution of PR-containing microorgan- isms in marine environments throughout the world was designed. The community structure of PR microorganisms were also compared using PCR assays and UniFrac analyses of 12 samples collected from three water layers （0, 75, and 200 m） at four representative sites in the Pacific, Atlantic, and Indian Oceans. The results indicate that PR- containing microorganisms can be grouped into two distribution types： widespread and location-specific. Representative cases of the former include SARll-PR and HOT2C01-PR. Interestingly, PR communities cluster by geographic locale but not by water depth.展开更多
Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody a...Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM, and the lymphokine activated killer(LAK) activity was tested simultaneously by isotope technique. Results: FAM could promote the proliferation of lymphocytes induced by ConA, raise the T cell count and regulate the T cell subsets disorder, elevate the LAK activity induced by IL 2. Conclusion: FAM possesses the effect of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides the experimental basis for clinical application of FAM.展开更多
文摘Proteorhodopsin （PR） is a recently discovered protein involved in the utilization of light energy. Several studies have shown that PR-containing microorganisms are widespread and compose a large proportion of the biomass in marine ecosystems. A better understanding of the ecological role of PR will help clarify the effect of the global flow of energy and the carbon cycle on marine communities. In this study, a bioinformatical database of PR codon sequences, the Global Distribution Database of Proteorhodopsin （GDDP）, as a tool for analyzing the diversity and distribution of PR-containing microorgan- isms in marine environments throughout the world was designed. The community structure of PR microorganisms were also compared using PCR assays and UniFrac analyses of 12 samples collected from three water layers （0, 75, and 200 m） at four representative sites in the Pacific, Atlantic, and Indian Oceans. The results indicate that PR- containing microorganisms can be grouped into two distribution types： widespread and location-specific. Representative cases of the former include SARll-PR and HOT2C01-PR. Interestingly, PR communities cluster by geographic locale but not by water depth.
文摘Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM, and the lymphokine activated killer(LAK) activity was tested simultaneously by isotope technique. Results: FAM could promote the proliferation of lymphocytes induced by ConA, raise the T cell count and regulate the T cell subsets disorder, elevate the LAK activity induced by IL 2. Conclusion: FAM possesses the effect of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides the experimental basis for clinical application of FAM.
