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A Neuro Metasurface Mode-Router for Fiber Mode Demultiplexing and Communications 被引量:1
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作者 Yu Zhao Huijiao Wang +10 位作者 Zile Li Tian Huang Chao Yang Ying Qiu Yuhan Gong Zhou Zhou Congling Liang Lei Yu Jin Tao Shaohua Yu Guoxing Zheng 《Engineering》 2025年第2期88-96,共9页
Advancements in mode-division multiplexing(MDM)techniques,aimed at surpassing the Shannon limit and augmenting transmission capacity,have garnered significant attention in optical fiber communica-tion,propelling the d... Advancements in mode-division multiplexing(MDM)techniques,aimed at surpassing the Shannon limit and augmenting transmission capacity,have garnered significant attention in optical fiber communica-tion,propelling the demand for high-quality multiplexers and demultiplexers.However,the criteria for ideal-mode multiplexers/demultiplexers,such as performance,scalability,compatibility,and ultra-compactness,have only partially been achieved using conventional bulky devices(e.g.,waveguides,grat-ings,and free space optics)—an issue that will substantially restrict the application of MDM techniques.Here,we present a neuro-meta-router(NMR)optimized through deep learning that achieves spatial multi-mode division and supports multi-channel communication,potentially offering scalability,com-patibility,and ultra-compactness.An MDM communication system based on an NMR is theoretically designed and experimentally demonstrated to enable simultaneous and independent multi-dataset transmission,showcasing a capacity of up to 100 gigabits per second(Gbps)and a symbol error rate down to the order of 104,all achieved without any compensation technologies or correlation devices.Our work presents a paradigm that merges metasurfaces,fiber communications,and deep learning,with potential applications in intelligent metasurface-aided optical interconnection,as well as all-optical pat-tern recognition and classification. 展开更多
关键词 Metasurfaces Deep learning Mode-division multiplexing Fiber communication
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Mobile blue-light communication over a signal optical path using a time-division multiplexing scheme 被引量:1
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作者 Pengzhan Liu Linning Wang +6 位作者 Jiayao Zhou Xinijie Mo Yingze Liang Jiahao Gou Ziqian Qi Ziping Cao Yongjin Wang 《Journal of Semiconductors》 2025年第3期82-88,共7页
Multiple quantum well(MQW) Ⅲ-nitride diodes that can simultaneously emit and detect light feature an overlapping region between their electroluminescence and responsivity spectra, which allows them to be simultaneous... Multiple quantum well(MQW) Ⅲ-nitride diodes that can simultaneously emit and detect light feature an overlapping region between their electroluminescence and responsivity spectra, which allows them to be simultaneously used as both a transmitter and a receiver in a wireless light communication system. Here, we demonstrate a mobile light communication system using a time-division multiplexing(TDM) scheme to achieve bidirectional data transmission via the same optical channel.Two identical blue MQW diodes are defined by software as a transmitter or a receiver. To address the light alignment issue, an image identification module integrated with a gimbal stabilizer is used to automatically detect the locations of moving targets;thus, underwater audio communication is realized via a mobile blue-light TDM communication mode. This approach not only uses a single link but also integrates mobile nodes in a practical network. 展开更多
关键词 multiple quantum well diodes electroluminescence and responsivity spectra overlap mobile light communication system time-division multiplexing scheme
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Dynamically Tunable THz Focusing via Mechanically Rotatable Cascaded Metasurfaces With Polarization Multiplexing
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作者 Hui Li Wenhui Xu +5 位作者 Hang Xu Jie Li Chunyu Song Qun Ren Yang Ren Jianquan Yao 《SmartSys》 2025年第3期1-9,共9页
Dynamically tunable terahertz(THz)beam focusing plays a critical role in emerging applications including reconfigurable imaging,localized spectral analysis,and micro-machining.Conventional methods,however,frequently e... Dynamically tunable terahertz(THz)beam focusing plays a critical role in emerging applications including reconfigurable imaging,localized spectral analysis,and micro-machining.Conventional methods,however,frequently employ complex wavefront modulators and external control algorithms,resulting in increased system footprint and limited tuning efficiency.In this work,we present an all-silicon mechanically rotatable cascaded metasurface capable of dynamic THz beam focusing.By independently adjusting the relative rotation angles between the two metasurface layers,real-time repositioning of the focal spot is achieved for orthogonal circular polarization channels.The proposed design facilitates polarization-division multiplexing without requiring external algorithms or active materials while preserving high focusing efficiency and beam quality across a predefined focal plane.Numerical simulations reveal a quasi-linear shift of the focal position with the rotation angle,with stable focusing efficiency and full-width at half-maximum observed in both polarization channels.This strategy offers an efficient and reliable approach to dynamic wavefront control for compact,reconfigurable THz imaging,sensing,and communication systems. 展开更多
关键词 cascaded metasurface dynamic beam focusing polarization-division multiplexing TERAHERTZ wavefront control
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Orbital angular momentum beams demultiplexing using a hybrid Fourier phase shift neural network
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作者 JIACHI YE TONGYAO WU +6 位作者 ABDULAZIZ BAZAMMUL QIAN CAI BELAL JAHANNIA ZIBO HU HAO WANG HAMED DALIR ELHAM HEIDARI 《Photonics Research》 2025年第12期I0017-I0029,共13页
The exponential growth in data traffic has driven significant research into maximizing the capacity of free-space optical(FSO)communication systems.Orbital angular momentum(OAM)multiplexing offers a promising approach... The exponential growth in data traffic has driven significant research into maximizing the capacity of free-space optical(FSO)communication systems.Orbital angular momentum(OAM)multiplexing offers a promising approach by using spatially structured beams with helical wavefronts to achieve higher data transmission rates.However,conventional electronic convolutional-neural-network-based OAM demultiplexing schemes exhibit substantial computational and energy efficiency limitations. 展开更多
关键词 spatially structured beams computational efficiency helical wavefronts angular momentum oam multiplexing data traffic hybrid fourier phase shift neural network free space optical communication DEmultiplexing
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Reflective metasurface connector for high-density and wideband space-division multiplexing
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作者 PENGJIU ZHAO JIANGBING DU +5 位作者 SHAOXING WANG LEYAN FEI TING LEI LUPING DU QUNBI ZHUGE ZUYUAN HE 《Photonics Research》 2025年第12期3522-3530,共9页
In this work,a reflective metasurface connector(RMC)and its application for high-density and wideband spacedivision multiplexing(SDM)are demonstrated.This device features single facet metasurface fabrication over an S... In this work,a reflective metasurface connector(RMC)and its application for high-density and wideband spacedivision multiplexing(SDM)are demonstrated.This device features single facet metasurface fabrication over an SOI platform based on a CMOS compatible process.A minimum fiber-to-fiber insertion loss of 2.9 dB at 1598.24 nm is realized for fan-in-fan-out(FIFO)applications between a single-mode fiber(SMF)array and a multicore fiber(MCF). 展开更多
关键词 WIDEBAND reflective metasurface connector rmc cmos compatible processa soi platform spacedivision multiplexing sdm multicore fiber mcf high density single facet metasurface fabrication
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双波长全光纤激光干涉速度复测技术
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作者 王为 刘盛刚 +4 位作者 谷伟 陶天炯 马鹤立 王翔 翁继东 《高压物理学报》 北大核心 2026年第3期31-37,共7页
针对冲击爆轰实验中对速度测量系统的高可靠性要求,基于光纤波分复用/解复用技术,提出了一种双波长全光纤激光干涉速度复测方法及技术。设计并搭建了一套速度复测原理验证系统,采用1530.3和1550.0 nm 2个波长,在气体炮上开展了低速、高... 针对冲击爆轰实验中对速度测量系统的高可靠性要求,基于光纤波分复用/解复用技术,提出了一种双波长全光纤激光干涉速度复测方法及技术。设计并搭建了一套速度复测原理验证系统,采用1530.3和1550.0 nm 2个波长,在气体炮上开展了低速、高速2种典型状态下的动态验证实验。实验结果显示,利用单一光纤探头实现了样品自由面运动速度的复测,2个波长系统得到的速度测量结果具有较好的一致性,速度相对偏差在±1.5%以内。 展开更多
关键词 激光干涉 多普勒测速 波分复用 速度复测
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波分复用光网络动态转发路由动态优化研究
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作者 涂芳 张晶伟 《激光杂志》 北大核心 2026年第1期137-142,共6页
为了有效提升波分复用光网络性能,针对波分复用光网络动态转发路由动态优化方法展开研究。建立波分复用光网络动态转发路由模型,在考虑波分复用光网络负载和节点距离约束条件下,将波分复用光网络剩余能量最大化、剩余能量方差和平均延... 为了有效提升波分复用光网络性能,针对波分复用光网络动态转发路由动态优化方法展开研究。建立波分复用光网络动态转发路由模型,在考虑波分复用光网络负载和节点距离约束条件下,将波分复用光网络剩余能量最大化、剩余能量方差和平均延迟最小化作为优化目标,构建波分复用光网络动态转发路由动态优化模型。采用改进猫群优化算法对模型求解,确定最优路由动态优化方案。实验结果表明,当业务数量呈现直线上升趋势时,所提方法的阻塞率最大值仅为0.22%,能够更高效地处理任务,提高系统的吞吐量,提升资源利用率,显著改善了波分复用光网络性能。 展开更多
关键词 波分复用光网络 动态转发 路由 动态优化
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High-SpeedReal-TimeDataAcquisitionSystem Realized by Interleaving/Multiplexing Technique 被引量:1
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作者 吕洁 莫毅群 罗伟雄 《Journal of Beijing Institute of Technology》 EI CAS 2000年第2期183-188,共6页
The interleaving/multiplexing technique was used to realize a 200?MHz real time data acquisition system. Two 100?MHz ADC modules worked parallelly and every ADC plays out data in ping pang fashion. The design improv... The interleaving/multiplexing technique was used to realize a 200?MHz real time data acquisition system. Two 100?MHz ADC modules worked parallelly and every ADC plays out data in ping pang fashion. The design improved the system conversion rata to 200?MHz and reduced the speed of data transporting and storing to 50?MHz. The high speed HDPLD and ECL logic parts were used to control system timing and the memory address. The multi layer print board and the shield were used to decrease interference produced by the high speed circuit. The system timing was designed carefully. The interleaving/multiplexing technique could improve the system conversion rata greatly while reducing the speed of external digital interfaces greatly. The design resolved the difficulties in high speed system effectively. The experiment proved the data acquisition system is stable and accurate. 展开更多
关键词 real-time data acquisition interleaving/multiplexing high-speed AD converter
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基于多重巢式聚合酶链式反应和毛细管电泳检测三种食源性致病菌
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作者 李俊杰 王惺雅 +5 位作者 杨浩楠 魏娜 赵丽颖 朱淑云 朱建华 何文森 《食品与发酵工业》 北大核心 2026年第4期242-250,共9页
该研究旨在利用多重巢式聚合酶链式反应(polymerase chain reaction,PCR)与毛细管电泳技术建立快速检测大肠埃希氏菌、鼠伤寒沙门氏菌和金黄色葡萄球菌的方法。该文以大肠埃希氏菌β-葡萄糖苷酶基因(uidA基因)、鼠伤寒沙门氏菌毒力基因(... 该研究旨在利用多重巢式聚合酶链式反应(polymerase chain reaction,PCR)与毛细管电泳技术建立快速检测大肠埃希氏菌、鼠伤寒沙门氏菌和金黄色葡萄球菌的方法。该文以大肠埃希氏菌β-葡萄糖苷酶基因(uidA基因)、鼠伤寒沙门氏菌毒力基因(hilA基因)和金黄色葡萄球菌耐热核酸酶基因(nuc基因)为靶标,设计3对嵌套式引物进行2轮扩增,利用毛细管电泳分析产物,通过对2轮巢式PCR退火温度的优化,建立3种食源性致病菌的多重巢式PCR-毛细管电泳检测方法。3对嵌套式引物特异性良好,2轮巢式PCR最佳退火温度分别为54℃和56℃,对大肠埃希氏菌和金黄色葡萄球菌检测灵敏度可达10^(1)copies,对鼠伤寒沙门氏菌检测灵敏度可达10^(3)copies。该方法快速、准确和高灵敏,适用于能提取DNA的食品样本中3种食源性致病菌的检测,为食源性致病菌的检测提供新方案。 展开更多
关键词 多重巢式PCR 毛细管电泳 食源性致病菌 特异性 灵敏度
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基于改进NSGA-Ⅱ的水声探通一体化正交频分复用波形优化算法
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作者 刘彦君 王宇杰 +2 位作者 王巍 陈春辉 黄海宁 《声学学报》 北大核心 2026年第2期491-500,共10页
面向水下探测通信一体化需求,本文以正交频分复用(OFDM)波形为基础,针对其峰均功率比以及非周期自相关积分旁瓣比较高的问题,提出一种基于改进第二代非支配排序遗传算法(NSGA-Ⅱ)的联合波形优化方法。该方法在恒模相位约束下,以预留子载... 面向水下探测通信一体化需求,本文以正交频分复用(OFDM)波形为基础,针对其峰均功率比以及非周期自相关积分旁瓣比较高的问题,提出一种基于改进第二代非支配排序遗传算法(NSGA-Ⅱ)的联合波形优化方法。该方法在恒模相位约束下,以预留子载波(TR)相位为优化变量,结合自适应交叉与变异策略以及少量精英个体的局部梯度调整,有效实现峰均功率比与积分旁瓣比协同抑制。仿真结果表明,与传统NSGA-Ⅱ算法相比,所提算法收敛更快、解集质量更高;与TRGerchberg–Saxton(TR-GS)算法、TR-Lp范数循环算法(TR-LNCA)等相比,所提方法能够获得更低的峰均功率比和积分旁瓣比,并同步降低通信误码率,提升弱目标检测概率。海试结果进一步验证了该方法的可行性。 展开更多
关键词 探通一体化 波形优化 正交频分复用 多目标优化
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生鲜乳中多重耐药基因液相芯片检测方法的建立与初步应用
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作者 向文君 赵格 +9 位作者 赵建梅 鲍钰莹 刘娜 王琳 张喜悦 宋时萍 高玉斌 刘俊辉 王君玮 田文霞 《中国动物检疫》 2026年第2期130-139,共10页
为建立一种可同时、快速、特异地检出生鲜乳中16种耐药基因的方法,针对生鲜乳中常见的9类16种耐药基因,从NCBI网站分别下载约30条序列,选择保守区域设计引物和探针,制备各耐药基因阳性质粒,并以其为模板建立液相芯片检测体系。结果显示... 为建立一种可同时、快速、特异地检出生鲜乳中16种耐药基因的方法,针对生鲜乳中常见的9类16种耐药基因,从NCBI网站分别下载约30条序列,选择保守区域设计引物和探针,制备各耐药基因阳性质粒,并以其为模板建立液相芯片检测体系。结果显示:建立的液相芯片技术可同时检测16种耐药基因,检测反应未出现相互干扰现象。对各基因的最低检出限:tetM为10^(0)copies/反应,blaZ、tetB、ant(4')-Ia、tetX和ermA为10copies/反应,floR、lnuA、sul1和mcr-1为10^(2)copies/反应,qnrB、blaNDM和addA为10^(3)copies/反应,ermB、mecA和blaSHV为10^(4)copies/反应。重复性试验的变异系数范围为0.97%~14.61%。临床样品检测结果显示,该方法与常规PCR、荧光PCR方法的符合率多在80%以上,且检出效果优于常规PCR及荧光PCR。结果表明,本研究建立的可同时快速检测生鲜乳中多种耐药基因的液相芯片方法具有特异性好、灵敏度高、重复性佳、检测范围广的优点,为生鲜乳中耐药基因监测提供了技术支撑。 展开更多
关键词 耐药基因 液相芯片 生鲜乳 Luminex xMAP 多重检测
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基于自适应直接二进制搜索算法的超紧凑型8通道模式-偏振复用器
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作者 马汉斯 林振 +9 位作者 何新 侯海良 李雪垒 贺龙辉 甘龙飞 赵娜 许辉 周灵钧 吴加贵 杨俊波 《物理学报》 北大核心 2026年第4期243-252,共10页
针对模式复用器难以兼容低损耗、小尺寸、多通道的难题,本文结合数字结构与波导结构,设计、加工、测试了一种超紧凑8通道模式-偏振复用器,以大幅度提升光互连的集成度和传输容量.数字结构是由新颖的自适应直接二进制搜索(adaptive direc... 针对模式复用器难以兼容低损耗、小尺寸、多通道的难题,本文结合数字结构与波导结构,设计、加工、测试了一种超紧凑8通道模式-偏振复用器,以大幅度提升光互连的集成度和传输容量.数字结构是由新颖的自适应直接二进制搜索(adaptive direct binary search,ADBS)算法优化,用于TE_(0),TE_(1),TE_(2)和TE_(3)模式的复用和解复用.ADBS算法通过引入池化算子和变异算子可以自适应地调节局部收敛,在保障器件小尺寸的前提下,大幅降低优化时间成本.波导结构是由非对称定向耦合器(asymmetrical directional coupler,ADC)设计,用于TM_(1),TM_(2),TM_(3)和TM_(4)模式的复用和解复用.ADC结构通过级联策略,在数字结构的基础上,进一步扩展通道数量.基于现有研究来看,所设计数字-波导结构有效长度约为100μm,是尺寸最小的8通道复用器.测试结果显示,在1550 nm处,测量的插入损耗和串扰分别小于1.2 dB和-12.5 dB.同时,在1540—1560 nm波长内,插入损耗和串扰分别小于1.7 dB和-9.3 dB.此外,在256 Gbps(1 Gbps=125 MB/s)数据传输实验中,测试所得眼图呈现出清晰且开放的特征,直观地反映出该器件具有优异的数据传输能力. 展开更多
关键词 8通道模式-偏振复用器 自适应直接二进制搜索算法 非对称定向耦合器
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Traits improvement of wild rice O. rufipogon via multiplex genome editing
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作者 Chang Tian Xu Tang +11 位作者 Guangzhong Zhang Rui Zhang Xinruolan Yang Lei Ding Caiyun Yin Hongfei Lin Fenglin Su Suikang Wang Xiaoxia Li Lianguang Shang Yong Zhang Quan Wang 《Journal of Integrative Plant Biology》 2026年第1期20-22,共3页
Rice, a global staple food, is critical for food security. The cultivated Oryza sativa, domesticated from wild O. rufipogon, derives~80%of its 993 identified domestication-related genes from O. rufipogon and 20%from S... Rice, a global staple food, is critical for food security. The cultivated Oryza sativa, domesticated from wild O. rufipogon, derives~80%of its 993 identified domestication-related genes from O. rufipogon and 20%from South/Southeast Asian wild O. nivara(Jing et al., 2023). Genes like An-1, BH4, PROG1,SH4, Rc, Rd, and GS3—which regulate awn length, hull color,til er angle, seed shattering, pericarp color, seed length, and thousand-grain weight, respectively—were selected against during domestication to form modern O. sativa(Yu et al., 2021).However, domestication and yield-focused breeding eliminated wild rice's valuable genes(e.g., for disease resistance, stress tolerance, nutrition), narrowing genetic diversity and impeding efforts to meet growing societal demands. 展开更多
关键词 Oryza sativa cultivated oryza sativa genetic diversity Oryza rufipogon wild rice multiplex genome editing disease resistance food security
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Rapid multiplex pathogen detection using 96-channel microfluidic chip with magnetic bead method
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作者 Enjia Zhang Jiaying Cao +6 位作者 Jianxin Cheng Gaozhe Cai Shuyue Jiang Weiwei Xie Chunping Jia Jianlong Zhao Shilun Feng 《Chinese Chemical Letters》 2026年第2期635-642,共8页
The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a mol... The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission.This study introduces a 96-channel microfluidic chip,fabricated through a molding process,which enables the batch detection of pathogens.It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction,elution,and amplification are completed entirely within the chip.This chip can extract nucleic acids from samples in just 10 min,achieving an extraction efficiency comparable to that of traditional in-tube methods.An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination.This approach allows for the simultaneous detection of 21 common respiratory pathogens,with a detection limit of 10 copies per reaction.Furthermore,applications involving clinical samples demonstrate significant practicality.Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips,this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity. 展开更多
关键词 96-Channel microfuild chip Multiplex pathogen detection Magnetic bead method Respiratory pathogens
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基于块差分编码的正交频分复用水声通信系统
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作者 张译元 武岩波 朱敏 《声学学报》 北大核心 2026年第2期649-661,共13页
在正交频分复用(OFDM)系统中,块差分编码方案中的差分调制能够在信道状态未知的情况下实现低复杂度鲁棒通信,其编码过程带来的分集增益可有效提升抗衰落性能。然而,水声信道的强时频双选性与低信噪比仍是限制当前系统应用的主要问题。... 在正交频分复用(OFDM)系统中,块差分编码方案中的差分调制能够在信道状态未知的情况下实现低复杂度鲁棒通信,其编码过程带来的分集增益可有效提升抗衰落性能。然而,水声信道的强时频双选性与低信噪比仍是限制当前系统应用的主要问题。本文提出一种基于块差分编码的OFDM水声通信系统,在发射端使用频域块差分编码方案,并与多进制Turbo码级联,以提高低信噪比条件下的可靠性;针对信道双选性造成相邻子载波相关系数下降的问题,提出一种基于子载波相关系数估计值的多进制差分符号对数似然矢量计算方法。仿真结果表明,在时变多径水声信道下,所提方案在频带利用率为0.5(bit/s)/Hz和1(bit/s)/Hz时相比同速率的差分相移键控和相移键控方案存在4 dB和2 dB增益。海试结果表明,所提方案在频带利用率为0.5(bit/s)/Hz和1(bit/s)/Hz时,正确译码的信噪比门限分别为7 dB和11 dB。 展开更多
关键词 块差分编码 符号级Turbo码 正交频分复用 频率分集
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多芯光纤分布式振动传感系统入侵定位性能研究
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作者 王佳蓓 顾宏灿 +2 位作者 姚高飞 刘文 黄俊斌 《海军工程大学学报》 北大核心 2026年第1期85-90,共6页
多芯光纤具有多个独立通道并行传感的特点,可通过其实现信号的空分复用,有效降低单芯光纤分布式振动传感系统的噪声水平、提升信号信噪比。本文首先从理论上验证了信号累加对提升信噪比的有效性,以及累加个数与信噪比增益呈指数关系;然... 多芯光纤具有多个独立通道并行传感的特点,可通过其实现信号的空分复用,有效降低单芯光纤分布式振动传感系统的噪声水平、提升信号信噪比。本文首先从理论上验证了信号累加对提升信噪比的有效性,以及累加个数与信噪比增益呈指数关系;然后,建立了多芯光纤零差探测型分布式振动传感系统,将探测光分为三束,分别注入多芯光纤的3个纤芯中同时并行传感;最后,对三芯解调信号进行累加,分析累加前后系统的探测性能。实验结果表明:与传统单通道零差探测系统相比,三芯信号的空分复用有效降低了系统噪声水平,信号信噪比提升了4.69 dB,扰动定位的均方根误差为0.52 m。可见,多芯光纤具备的多路信号空分复用的优势,为分布式光纤振动传感系统的发展提供了新方向。 展开更多
关键词 多芯光纤 分布式振动传感 信噪比 空分复用 扰动定位
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鸽源病毒多重TaqMan实时荧光定量PCR检测方法的建立及应用
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作者 安乐乐 宗保君 +2 位作者 任晓婷 张业涛 赵永清 《微生物学通报》 北大核心 2026年第1期499-518,共20页
【背景】鸽腺病毒(pigeon adenovirus,PiAdV)、鸽圆环病毒(pigeon circovirus,PiCV)与鸽疱疹病毒(pigeon herpesvirus,PiHV)是严重危害鸽群健康的主要病原体,常导致混合感染,给临床鉴别诊断带来困难。【目的】建立一种可用于同步检测Pi... 【背景】鸽腺病毒(pigeon adenovirus,PiAdV)、鸽圆环病毒(pigeon circovirus,PiCV)与鸽疱疹病毒(pigeon herpesvirus,PiHV)是严重危害鸽群健康的主要病原体,常导致混合感染,给临床鉴别诊断带来困难。【目的】建立一种可用于同步检测PiAdV、PiCV和PiHV的三重TaqMan荧光定量PCR(real-time quantitative PCR,RT-qPCR)方法。【方法】通过查找GenBank发布的PiAdV、PiCV和PiHV基因组并加以比对获取其基因保守区域,在保守区域设计引物、探针和制备质粒标准品pMD19-T-PiAdVI、pMD19-T-PiAdVII、pMD19-T-PiCV及pMD19-T-PiHV;经反应体系优化,标准曲线绘制,评价方法的特异性、敏感性和重复性,建立可同步检测PiAdV、PiCV和PiHV的三重TaqMan RT-qPCR方法;使用该方法进行临床样本检测以评估其临床适用性。【结果】该PiAdV、PiCV和PiHV的三重TaqMan RT-qPCR方法标准曲线为y=–3.396x+43.210(R2=0.9983)、y=–3.540x+40.540(R2=0.9994)和y=–3.280x+38.470(R2=0.9984),线性关系良好;与鸽痘病毒、鸽轮状病毒等鸽子其他常见病原及禽腺病毒4型无交叉反应,特异性强;在敏感性方面,该方法的最低检出限分别为10 copies/μL、10 copies/μL和1 copy/μL,敏感度是常规PCR方法的100-1000倍,敏感性高;批内、批间重复性试验的变异系数均小于2.0%,重复性和稳定性良好。使用建立的PiAdV、PiCV和PiHV的三重TaqMan RT-qPCR检测方法对152份鸽子临床样本进行检测,RT-qPCR阳性检出率(PiAdV55.92%、PiCV 51.32%、PiHV 40.13%)略高于常规PCR方法,具有良好的临床适用性。【结论】本研究建立的PiAdV、PiCV和PiHV的三重TaqMan RT-qPCR检测方法标准曲线的线性关系良好、特异性强、敏感性高、重复性良好,可快速、高效、特异检测临床样本中的PiAdV、PiCV和PiHV,为PiAdV、PiCV和PiHV早期诊断、协同致病机制研究和流行病学调查提供一种高效的技术手段。 展开更多
关键词 鸽腺病毒 鸽圆环病毒 鸽疱疹病毒 多重TaqMan RT-qPCR 早期诊断 流行病学调查
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New Passenger Services in Railway Stations Based on Mode Group Diversity Multiplexing Optical Fiber Communications
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作者 Dafa Loum Charles Tatkeu +2 位作者 Iyad Dayoub Marc Heddebaut Jean Michel Rouvaen 《Journal of Civil Engineering and Architecture》 2012年第10期1362-1368,共7页
Nowadays, because of its wide bandwidth and high communication capability, the optical fiber is more and more used for high data rate transmission of information in railway environments. Conventionally, only one servi... Nowadays, because of its wide bandwidth and high communication capability, the optical fiber is more and more used for high data rate transmission of information in railway environments. Conventionally, only one service is sent over the fiber at a time. However, many different services can be simultaneously conveyed in railway stations such as passenger information service, cellular phone, Wi-Fi... The objective of the work proposed in this paper is to demonstrate the potential benefits of transmitting radio signals over fiber in a railway environment. The main idea is to exploit the full capacity of the fiber by transmitting multiple services using the same fiber. Since, different services are operating in different frequency bands; we propose a new multiplexing technique called Mode Group Diversity Multiplexing (MGDM) to ensure the transmission of multiple services using the same fiber, without additional infrastructure. There are numerous advantages of the proposed technique, e.g., faster and reliable data exchange, high resolution video surveillance capability, high data rate information exchange in railway stations. We present, in this paper, the physical characteristics of optical fibers, performance of MGDM multiplexing technique, and the influence of the laser excitation conditions at the entrance of the fiber on the performances of the system. 展开更多
关键词 Optical fiber passenger services railway station mode group diversity multiplexing.
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基于单水听器的浅水近距离正交频分复用信源最大似然定位
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作者 曲星昊 乔钢 商志刚 《声学学报》 北大核心 2026年第2期548-558,共11页
针对水下小型平台对通信感知一体化的需求,研究了基于单水听器的正交频分复用信源定位方法。根据多径信道下通信系统的频域输入输出关系,结合导频信息构建了用于目标定位的量测模型。发展了基于多径相对时延的匹配场定位方法,并开发了... 针对水下小型平台对通信感知一体化的需求,研究了基于单水听器的正交频分复用信源定位方法。根据多径信道下通信系统的频域输入输出关系,结合导频信息构建了用于目标定位的量测模型。发展了基于多径相对时延的匹配场定位方法,并开发了一种迭代求解算法,其中首达路径的时偏估计与声源的距离–深度估计交替执行。在典型的浅水三径信道下,推导了系统定位误差的克拉美罗界。数值仿真验证了所提算法在理想条件下的统计有效性,并围绕环境参数失配进行了算法敏感性分析。水池试验中,与多径时延匹配方法相比,所提算法给出了更为精确、可靠的定位结果。 展开更多
关键词 正交频分复用 信源定位 单水听器 多径信道
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多重PCR在血流感染细菌及其耐药基因检测中的临床应用价值
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作者 曾桂胜 陈丹虹 +3 位作者 吴见欢 陈志芬 邹惠英 廖建军 《齐齐哈尔医学院学报》 2026年第3期264-269,共6页
目的评价多重PCR法在检测血流感染细菌及其耐药基因中的临床应用价值。方法使用血流感染细菌及其耐药基因15项联合检测试剂盒,采用多重PCR法于体外同时检测50例报阳血液培养中的12种临床常见血流感染病原菌及KPC、mecA、NDM 3种耐药基因... 目的评价多重PCR法在检测血流感染细菌及其耐药基因中的临床应用价值。方法使用血流感染细菌及其耐药基因15项联合检测试剂盒,采用多重PCR法于体外同时检测50例报阳血液培养中的12种临床常见血流感染病原菌及KPC、mecA、NDM 3种耐药基因,与传统血培养方法比较其检出时效性,准确性。结果共检测出表皮葡萄球菌、铜绿假单胞菌、人葡萄球菌、金黄色葡萄球菌、粪肠球菌、大肠埃希菌、嗜麦芽单胞菌、肺炎链球菌、鲍曼不动杆菌的,未检出溶血葡萄球菌、屎肠球菌、肺炎克雷伯菌和KPC、mecA、NDM等耐药基因。相较传统血培养方法,多重PCR法对血培养阳性患者的TAT时间可缩短1~2 d,及早提示感染病原菌。结论与传统血培养细菌鉴定相比,联合检测试剂盒体现出多重PCR的敏感性、特异性、阳性预测值较高,可提高血流感染细菌检测速度,对多重感染的鉴定及指导临床早期合理用药具有意义。 展开更多
关键词 血流感染 细菌培养 多重PCR 耐药基因
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