In this paper,analyzed is the symbol synchronization algorithm in orthogonal frequency division multiplex(OFDM)system,and accomplished are the hardware circuit design of coarse and elaborate synchronization algorithms...In this paper,analyzed is the symbol synchronization algorithm in orthogonal frequency division multiplex(OFDM)system,and accomplished are the hardware circuit design of coarse and elaborate synchronization algorithms.Based on the analysis of coarse and elaborate synchronization algorithms,multiplexed are,the module accumulator,division and output judgement,which can evidently save the hardware resource cost.The analysis of circuit sequence and wave form simulation of the design scheme shows that the proposed method efficiently reduce system resources and power consumption.展开更多
Rice blast is one of the important diseases in major rice producing areas of China. The main blast resistance genes Pi-ta and Pi-b showed broad-spectrum and durable resistance to rice blast in many rice growing areas ...Rice blast is one of the important diseases in major rice producing areas of China. The main blast resistance genes Pi-ta and Pi-b showed broad-spectrum and durable resistance to rice blast in many rice growing areas of China, which have been widely utilized in rice breeding and commercial production. In this study, on the basis of detection and verification of the genotypes of 22 rice varieties har- boring known blast resistance genes （Pi-ta and Pi-b） and blast susceptibility genes （pi-ta and pi-b）, two multiple PCR systems for these genes were established by us- ing the functional markers of blast resistance genes Pi-ta and Pi-b as well as blast susceptibility genes pi-ta and pi-b, respectively. Specifically, multiple PCR system I could simultaneously detect blast resistance genes Pi-ta and Pi-b, while system II could detect simultaneously blast susceptibility genes pi-ta and pi-b. In addition, the genotypes of 336 high generation breeding materials were detected with these two multiple PCR systems. The results were highly consistent with those of conventional single mark detection, indicating that these two multiplex PCR systems were stable, reliable and time-saving. The established multiplex PCR systems may serve as a rapid and efficient method to identify and screen rice germplasm resources and can be applied in marker-assisted selection to polymerize multiple genes for blast resis- tance in rice breeding.展开更多
[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citru...[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citrus, the primers specific to CaMV35S promoter, NOS promoter, NOS terminator and actin gene were designed, to establish a multiple PCR system which could detect four types of sequences. In addition, orthogonal tests were performed to determine the optimal concentrations of all the components in PCR reaction system, as well as the optimal PCR cycle parameters. [Result] The optimal PCR reaction system should contain 2.5μl of 10xPCR buffer, 2.0μl of MgCI2 （25 mmol/L）, 2.0 μl of dNTP mixture （2.5 mmol/L of each dNTP）, 1.0 μl of actin gene primers （10μmol/L）, 1.0μl of 35S promoter primers （10 μmol/L）, 1.5 μl of NOS promoter primers （10 μmol/L） and 0.5 μl of NOS terminator primers （10μmol/L）, 0.1 μg of template DNA, 1.25 U of Taq DNA polymerase; ddH20 was added to the total reaction system of 25μl. The PCR reaction program consisted of pre-denaturing at 94℃ for 5 min; 31 cycles of denaturing at 94℃ for 30 s, annealing at 64.1℃ for 45 s and extension at 72℃ for 50 s; final extension at 72℃ for 10 min. The reaction system optimized with the orthogonal tests could detect as less as 0.1% transgenic component in the tested samples. [Conclusion] The MPCR detection system established in this study can meet the requirements in theory for detecting the genetically modified ingredients in Citrus or the deep-processed products.展开更多
The interleaving/multiplexing technique was used to realize a 200?MHz real time data acquisition system. Two 100?MHz ADC modules worked parallelly and every ADC plays out data in ping pang fashion. The design improv...The interleaving/multiplexing technique was used to realize a 200?MHz real time data acquisition system. Two 100?MHz ADC modules worked parallelly and every ADC plays out data in ping pang fashion. The design improved the system conversion rata to 200?MHz and reduced the speed of data transporting and storing to 50?MHz. The high speed HDPLD and ECL logic parts were used to control system timing and the memory address. The multi layer print board and the shield were used to decrease interference produced by the high speed circuit. The system timing was designed carefully. The interleaving/multiplexing technique could improve the system conversion rata greatly while reducing the speed of external digital interfaces greatly. The design resolved the difficulties in high speed system effectively. The experiment proved the data acquisition system is stable and accurate.展开更多
Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions, or for analyzing epistatic re- lationships in genetic pathways. Using ...Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions, or for analyzing epistatic re- lationships in genetic pathways. Using conventional methods, mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring. This process is both timeconsuming and labor-intensive. Moreover, if the genes of interest are closely linked, multiple mutations can not be generated （Wijnker and de Jong, 2008）.展开更多
Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. ...Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components （enzyme, MgCl2, DNA template and dNTPs） in PCR system were used in the research with the concentration of the other component remained the same; the orthogonal design L9 （34） was applied in the optimization of four sets of primers （STM0014, Pat, SSI, and UGP） in the reaction system at three levels; the temperature gradient selection was used to find out the optimum annealing temperature for the primer. The optimized multiplex PCR system of potato SSR marker with a total volume of 20 μL ： 2.5 μL 25 mmol.L-1 MgCl2, 0.6 μL 10 mmol·L-1 dNTPs, 0.8 U Taq, 80 ng DNA template was ultimately established through the comparison and analysis of test results; the ratio of four pairs of 4 mmol. L1 primers was 2 ： 1 ： 2 ： 3, and the annealing temperature was 54.7℃. The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties. This research built the solid foundation for the further study of genetic diversity of potato germplasms and construction of DNA fingerprinting..展开更多
Atmospheric turbulence（AT） induced crosstalk can significantly impair the performance of a free-space optical（FSO）communication link using orbital angular momentum（OAM） multiplexing.In this paper,we propose a mu...Atmospheric turbulence（AT） induced crosstalk can significantly impair the performance of a free-space optical（FSO）communication link using orbital angular momentum（OAM） multiplexing.In this paper,we propose a multiple-user detection（MUD） turbulence mitigation scheme in an OAM-multiplexed FSO communication link.First,we present a MUD equivalent communication model for an OAM-multiplexed FSO communication link under AT.In the equivalent model,each input bit stream represents one user＇s information.The deformed OAM spatial modes caused by AT,instead of the pure OAM spatial modes,are used as information carriers,and the overlapping between the deformed OAM spatial modes are computed as the correlation coefficients between the users.Then,we present a turbulence mitigation scheme based on MUD idea to enhance AT tolerance of the OAM-multiplexed FSO communication link.In the proposed scheme,the crosstalk caused by AT is used as a useful component to deduce users＇ information.The numerical results show that the performance of the OAM-multiplexed communication link has greatly improved by the proposed scheme.When the turbulence strength C_n^2 is 1 × 10^（-15） m^（-2/3）,the transmission distance is 1000 m and the channel signal-to-noise ratio（SNR）is 26 dB,the bit-error-rate（BER） performance of four spatial multiplexed OAM modes l_m = ＋ 1,＋2,＋3,＋4 are all close to 10-5,and there is a 2-3 fold increase in the BER performance in comparison with those results without the proposed scheme.In addition,the proposed scheme is more effective for an OAM-multiplexed FSO communication link with a larger OAM mode topological charge interval.The proposed scheme is a promising direction for compensating the interference caused by AT in the OAM-multiplexed FSO communication link.展开更多
This paper expounds the necessity of constructing China's rural multiplex financial system as follows.First,overcome the bottleneck effect arising from the shortage of capital in the process of development of rura...This paper expounds the necessity of constructing China's rural multiplex financial system as follows.First,overcome the bottleneck effect arising from the shortage of capital in the process of development of rural small and medium-sized enterprise,and increase farmers' income;second,provide capital support for rural modern industrial development,and promote rural economic development;third,rationally allocate capital,master the money flow and coordinate urban-rural development.The paper also profoundly analyzes the difficulties of constructing China's rural multiplex financial system as follows.First,the existing rural financial system structure is irrational and the function is unsound;second,non-governmental financial market is disorderly and the organization is unsound;third,the rural financial system is simple and the overhaul of rural credit cooperatives system has not yet been done;fourth,the construction of rural system of financial laws and regulations is laggard,and the construction of rural multiplex financial system lacks good social environment.The specific thoughts,measures and countermeasures of constructing mechanism innovation of China's rural multiplex financial system are put forward as follows.First,increasingly perfect legal system and provide legal support for constructing China's rural multiplex financial system;second,quicken the overhaul of rural credit cooperatives system and perfect its function in China's rural multiplex financial system;third,strengthen the degree of support and development,and change the situation of simple structure of rural financial system;fourth,reinforce administrative supervision and perfect public services,and standardize the rural financial market order.展开更多
The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of t...The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.展开更多
In recent years, the booming band- width demands of dedicated mobile services have driven the rapid development of optical transport networks （OTNs）. Through the in- novative use of emerging coherent optical communi...In recent years, the booming band- width demands of dedicated mobile services have driven the rapid development of optical transport networks （OTNs）. Through the in- novative use of emerging coherent optical communication technology and the advance- ment of microelectronics technology, the new-generation 100Gb/s transport technology offers a high line rate and unprecedented re- silience to optical transmission impairments. This paper overviews the bandwidth demands of China Mobile driven by the upcoming roll- out of Time Division-Long Term Evolution （TD-LTE） and presents the 100Gb/s trials at China Mobile that were used to verify the performance of a lOOGb/s system. China Mo- bile＇s considerations, which were based on the trial results, regarding the deployment of lOOGb/s transport systems are introduced, and the requirements of China Mobile for the evo- lution of lOOGb/s transport systems are sum- marized.展开更多
In order to rapidly diagnose and differentiate tuberculosis from other bacterial infections, a 16S rRNA gene (16s rDNA)-directed multiplex PCR system was developed. In this system, a pair of universal primers and a ...In order to rapidly diagnose and differentiate tuberculosis from other bacterial infections, a 16S rRNA gene (16s rDNA)-directed multiplex PCR system was developed. In this system, a pair of universal primers and a tubercle bacillus (Tb)-specific primer were designed based on highly con- served regions and Tb species-specific variable region of bacterial 16s rDNA. A 360bp fragment was detected in all bacteria tested, and a 210bp fragment was found only in Tb. 19 species of known bac- teria including Tb were used for evaluating specificity, universality and sensitivity of the PCR. Candi- da albicans and human diploid cell served as controls. It was found that both 210bp and 360bp frag- ments were amplified only in Tb, and only 360 bp fragment was detected in other 18 species of gener- al bacteria. Candida albicans and human cells were negative for both 360bp and 2l0bp fragments. The lowest detectable level of the PCR was 10 fg of DNA for Escherichia coli and 100 fg of DNA for Tb. The results indicated that this multiplex PCR system for the simultaneous detection of Tb and other common bacteria had higher specificity and sensitivity, as well as good universality and might be useful to rapidly diagnose bacterial infections and effectively distinguish tuberculosis from other bacterial involvement.展开更多
Quadrature Spatial Modulation (QSM) is a high spectral efficiency Multiple-Input Multiple Output (MIMO) technique used to improve the spectral efficiency of wireless communication systems. The main concept of QSM is t...Quadrature Spatial Modulation (QSM) is a high spectral efficiency Multiple-Input Multiple Output (MIMO) technique used to improve the spectral efficiency of wireless communication systems. The main concept of QSM is to extend the spatial constellation of the conventional Spatial Modulation (SM) in both the in-phase and quadrature components of the data symbol. In this paper, because QSM-based on Interleaxdng Division Multiplexing (IDM) has not been introduced in the literature as a multiple antenna system, we introduced a novel scheme, called QSM system based on Interleaving Division Multiplexing (QSM-IDM). The antenna sets are also applied to a spreader, before being used to assign an antenna number for information transmission. Analysis and simulations for a flat fading channel show that the proposed QSM-IDM method significantly outperforms the original QSM system with the same data rate, while maintaining a relatively acceptable complexity. The obtained simulation results show that the conducted analysis yields significant improvements for the accuracy of the proposed scheme, with satisfactory complexity.展开更多
This paper begins with Nyquist wavelengthdivision multiplexing （WDM） and then introduces fasterthanNyquist. In fasterthanNyquist a certain amount of intersymbol interference （ISI） is accepted, which violates the f...This paper begins with Nyquist wavelengthdivision multiplexing （WDM） and then introduces fasterthanNyquist. In fasterthanNyquist a certain amount of intersymbol interference （ISI） is accepted, which violates the fundamental principle of Nyquist WDM. This results in muchrelaxed transceiver bandwidth and simpler spectral design. However, in fasterthanNyquist, implementation complexity is shifted from the transmitter side to the receiver side. Therefore, successful application of fasterthanNyquist depends on innovation in the receiver structure. In this paper, we discuss the guidelines for implementing suboptimum, lowcomplexity receivers based on fasterthanNyquist. We suggest that duobinary shaping is a good technique for trading off achievable spectral efficiency, detection performance, and implementation complexity and might be preferable to Nyquist WDM. Experiments are conducted to verify robustness of the proposed technique.展开更多
In this paper, an experimental scheme for cordwood color division multiplexing(CDM) visible light communication(VLC) system is proposed. The principle and structure of the experimental scheme are described. Right angl...In this paper, an experimental scheme for cordwood color division multiplexing(CDM) visible light communication(VLC) system is proposed. The principle and structure of the experimental scheme are described. Right angle prisms and band-pass filter are used to make the unit of the optical collector and splitter. We can add or subtract the unit as we need. The cordwood CDM-VLC system can also be effectively used as a model to accomplish color shift keying and color division duplexing. The experimental system provides a new way for researching VLC.展开更多
The performance of the wavelength division multiplexing (WDM) photonic analogue-to-digital converter (ADC) used for digitization of high-resolution radar systems is evaluated numerically by using the peak signal-to-no...The performance of the wavelength division multiplexing (WDM) photonic analogue-to-digital converter (ADC) used for digitization of high-resolution radar systems is evaluated numerically by using the peak signal-to-noise ratio (SNR) metric. Two different WDM photonic ADC architectures are considered for the digitization of radar signals with 5 GHz of bandwidth (spatial resolution of 3 cm), in order to provide a comprehensive study of the compromises present when deploying radar signals with high-resolution: 1) a four-channel architecture with each channel employing an ADC with 5 GSamples/s, and 2) an eight-channel architecture with each channel employing an ADC with 2.5 GSamples/s. For peak powers of the pulsed source between 10 and 20 dBm and a distance between the radar antenna and the sensing object of 2.4 meters, peak SNR levels between 29 and 39 dB are achieved with the eight-channel architecture, which shows higher peak SNR levels when compared with the four-channel architecture. For the eight-channel architecture and for the same peak powers of the pulsed source, peak SNR levels between 11 and 16 dB are obtained when the distance increases to 13.5 meters. With this evaluation using the peak SNR, it is possible to assess the performance limits when choosing a specific radar range, while keeping the same resolution.展开更多
This paper presents the fundamental technique of phase generated carrier （PGC） and its realization on compact reconfigurable input and output （RIO） which adopts real-time and field programmable grate array （FPGA...This paper presents the fundamental technique of phase generated carrier （PGC） and its realization on compact reconfigurable input and output （RIO） which adopts real-time and field programmable grate array （FPGA） techniques. Improvement of the PGC technique is also introduced by using peak-to-peak value detection method to reduce the influence of variation of the light intensity. A four-element fibre Bragg gratings （FBG） laser sensor system is conducted in the experiment and the demodulated results demonstrate correlation coefficient as high as 0.995 with the reference signal and the dynamic range to be 120dB@63Hz.展开更多
We propose a novel and efficient multi-functional optical fiber sensor system based on a dense wavelength division multiplexer(DWDM).This system consists of an optical fiber temperature sensor, an optical fiber strain...We propose a novel and efficient multi-functional optical fiber sensor system based on a dense wavelength division multiplexer(DWDM).This system consists of an optical fiber temperature sensor, an optical fiber strain sensor, and a 48-channel DWDM.This system can monitor temperature and strain changes at the same time.The ranges of these two sensors are from-20℃ to 100℃ and from-1000 με to 2000 με, respectively.The sensitivities of the temperature sensor and strain sensor are 0.03572 nm/℃ and 0.03808 nm/N, respectively.With the aid of a broadband source and spectrometer,different kinds and ranges of parameters in the environment can be monitored by using suitable sensors.展开更多
Three clock synchronization schemes for a quantum key distribution system are compared experimentally through the outdoor fibre and the interaction physical model of the the clock signal and the the quantum signal in ...Three clock synchronization schemes for a quantum key distribution system are compared experimentally through the outdoor fibre and the interaction physical model of the the clock signal and the the quantum signal in the quantum key distribution system is analysed to propose a new synchronization scheme based on time division multiplexing and wavelength division multiplexing technology to reduce quantum bits error rates under some transmission rate conditions, The proposed synchronization scheme can not only completely eliminate noise photons from the bright background light of the the clock signal, but also suppress the fibre nonlinear crosstalk.展开更多
Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (...Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (FHCM).A microarray was fabricated to screen mutations in exons 3,5,7,and 8 in cTnI gene.Primers were designed for the PCR (polymerase chain reaction) to amplify the target DNA fragments from fresh blood samples.In order to simplify the PCR process,multiplex PCR technology was investigated in detail.The concentration of Mg^(2+) played an important role in multiplex PCR process,a properly low concentration of Mg^(2+) submitted a better speciality of PCR products.The speciality was also favored when the annealing temperature was reasonably enhanced and 64℃is the optimal annealing temperature for the multiplex PCR systems.When applying the fabricated gene-chip to detect the target fragments from PCR mixture,the signal intensity sequence is in accordance with that from theoretic estimate.展开更多
An electronic digital equalizer for polarization multiplex coherent fiber optic communication systems is designed to compensate polarization mode dispersion (PMD) and residual chromatic dispersion (CD) of transmission...An electronic digital equalizer for polarization multiplex coherent fiber optic communication systems is designed to compensate polarization mode dispersion (PMD) and residual chromatic dispersion (CD) of transmission channel. The proposed equalizer is realized with fraction spaced infinite impulse response (IIR) butterfly structure with 21 feedforward taps and 2 feedback taps. Compared with finite impulse response (FIR) structure, this structure can reduce implementation complexity of hardware under the same condition. To keep track of the random variation of channel characteristics, the filter weights are updated by least mean square (LMS) algorithm. The simulation results show that the proposed equalizer can compensate residual chromatic dispersion (CD) of 1600 ps/nm and differential group delay (DGD) of 90 ps simultaneously, and also can increase the PMD and residual CD tolerance of the whole communication system.展开更多
基金Guangdong Province Science and Technology Guiding Project(2005B10101013)
文摘In this paper,analyzed is the symbol synchronization algorithm in orthogonal frequency division multiplex(OFDM)system,and accomplished are the hardware circuit design of coarse and elaborate synchronization algorithms.Based on the analysis of coarse and elaborate synchronization algorithms,multiplexed are,the module accumulator,division and output judgement,which can evidently save the hardware resource cost.The analysis of circuit sequence and wave form simulation of the design scheme shows that the proposed method efficiently reduce system resources and power consumption.
基金Supported by Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province[CX(12)1003]Science and Technology Support Program of Jiangsu Province(BE2013301)Special Fund for the Construction of Modern Agriculture Industry System of China(CARS-01-47)~~
文摘Rice blast is one of the important diseases in major rice producing areas of China. The main blast resistance genes Pi-ta and Pi-b showed broad-spectrum and durable resistance to rice blast in many rice growing areas of China, which have been widely utilized in rice breeding and commercial production. In this study, on the basis of detection and verification of the genotypes of 22 rice varieties har- boring known blast resistance genes （Pi-ta and Pi-b） and blast susceptibility genes （pi-ta and pi-b）, two multiple PCR systems for these genes were established by us- ing the functional markers of blast resistance genes Pi-ta and Pi-b as well as blast susceptibility genes pi-ta and pi-b, respectively. Specifically, multiple PCR system I could simultaneously detect blast resistance genes Pi-ta and Pi-b, while system II could detect simultaneously blast susceptibility genes pi-ta and pi-b. In addition, the genotypes of 336 high generation breeding materials were detected with these two multiple PCR systems. The results were highly consistent with those of conventional single mark detection, indicating that these two multiplex PCR systems were stable, reliable and time-saving. The established multiplex PCR systems may serve as a rapid and efficient method to identify and screen rice germplasm resources and can be applied in marker-assisted selection to polymerize multiple genes for blast resis- tance in rice breeding.
基金Supported by the Special Fund for Key Laboratories of Chongqing (CSTC)National Technology Research and Development Program of Ministry of Science and Technology for Countryside Field (863 Program,2011AA100205)+1 种基金Special Fund for Agro-scientific Research in the Public Interest of Ministry of Agriculture of China(201003067)Key Science and Technology Research Program of Ministry of Education of China (109131)~~
文摘[Objective] This study aimed to establish a multiplex PCR system for de- tecting transgenic ingredients from Citrus. [Method] Based on the pBI121 plasmid sequences published in GenBank and actin gene sequence of Citrus, the primers specific to CaMV35S promoter, NOS promoter, NOS terminator and actin gene were designed, to establish a multiple PCR system which could detect four types of sequences. In addition, orthogonal tests were performed to determine the optimal concentrations of all the components in PCR reaction system, as well as the optimal PCR cycle parameters. [Result] The optimal PCR reaction system should contain 2.5μl of 10xPCR buffer, 2.0μl of MgCI2 （25 mmol/L）, 2.0 μl of dNTP mixture （2.5 mmol/L of each dNTP）, 1.0 μl of actin gene primers （10μmol/L）, 1.0μl of 35S promoter primers （10 μmol/L）, 1.5 μl of NOS promoter primers （10 μmol/L） and 0.5 μl of NOS terminator primers （10μmol/L）, 0.1 μg of template DNA, 1.25 U of Taq DNA polymerase; ddH20 was added to the total reaction system of 25μl. The PCR reaction program consisted of pre-denaturing at 94℃ for 5 min; 31 cycles of denaturing at 94℃ for 30 s, annealing at 64.1℃ for 45 s and extension at 72℃ for 50 s; final extension at 72℃ for 10 min. The reaction system optimized with the orthogonal tests could detect as less as 0.1% transgenic component in the tested samples. [Conclusion] The MPCR detection system established in this study can meet the requirements in theory for detecting the genetically modified ingredients in Citrus or the deep-processed products.
文摘The interleaving/multiplexing technique was used to realize a 200?MHz real time data acquisition system. Two 100?MHz ADC modules worked parallelly and every ADC plays out data in ping pang fashion. The design improved the system conversion rata to 200?MHz and reduced the speed of data transporting and storing to 50?MHz. The high speed HDPLD and ECL logic parts were used to control system timing and the memory address. The multi layer print board and the shield were used to decrease interference produced by the high speed circuit. The system timing was designed carefully. The interleaving/multiplexing technique could improve the system conversion rata greatly while reducing the speed of external digital interfaces greatly. The design resolved the difficulties in high speed system effectively. The experiment proved the data acquisition system is stable and accurate.
基金supported by the Agricultural Science and Technology Innovation Program
文摘Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions, or for analyzing epistatic re- lationships in genetic pathways. Using conventional methods, mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring. This process is both timeconsuming and labor-intensive. Moreover, if the genes of interest are closely linked, multiple mutations can not be generated （Wijnker and de Jong, 2008）.
基金Supported by the International Cooperation Project of Heilongjiang Science and Technology Department (WC05B08)
文摘Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components （enzyme, MgCl2, DNA template and dNTPs） in PCR system were used in the research with the concentration of the other component remained the same; the orthogonal design L9 （34） was applied in the optimization of four sets of primers （STM0014, Pat, SSI, and UGP） in the reaction system at three levels; the temperature gradient selection was used to find out the optimum annealing temperature for the primer. The optimized multiplex PCR system of potato SSR marker with a total volume of 20 μL ： 2.5 μL 25 mmol.L-1 MgCl2, 0.6 μL 10 mmol·L-1 dNTPs, 0.8 U Taq, 80 ng DNA template was ultimately established through the comparison and analysis of test results; the ratio of four pairs of 4 mmol. L1 primers was 2 ： 1 ： 2 ： 3, and the annealing temperature was 54.7℃. The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties. This research built the solid foundation for the further study of genetic diversity of potato germplasms and construction of DNA fingerprinting..
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61271238 and 61475075)the Open Research Fund of Key Lab of Broadband Wireless Communication and Sensor Network Technology,Ministry of Education,China(Grant No.NYKL2015011)+1 种基金the Postgraduate Innovation Research Plan of Jiangsu Province,China(Grant No.CXZZ13_0489)the University Natural Science Foundation of Jiangsu Province,China(Grant No.16KJB510037)
文摘Atmospheric turbulence（AT） induced crosstalk can significantly impair the performance of a free-space optical（FSO）communication link using orbital angular momentum（OAM） multiplexing.In this paper,we propose a multiple-user detection（MUD） turbulence mitigation scheme in an OAM-multiplexed FSO communication link.First,we present a MUD equivalent communication model for an OAM-multiplexed FSO communication link under AT.In the equivalent model,each input bit stream represents one user＇s information.The deformed OAM spatial modes caused by AT,instead of the pure OAM spatial modes,are used as information carriers,and the overlapping between the deformed OAM spatial modes are computed as the correlation coefficients between the users.Then,we present a turbulence mitigation scheme based on MUD idea to enhance AT tolerance of the OAM-multiplexed FSO communication link.In the proposed scheme,the crosstalk caused by AT is used as a useful component to deduce users＇ information.The numerical results show that the performance of the OAM-multiplexed communication link has greatly improved by the proposed scheme.When the turbulence strength C_n^2 is 1 × 10^（-15） m^（-2/3）,the transmission distance is 1000 m and the channel signal-to-noise ratio（SNR）is 26 dB,the bit-error-rate（BER） performance of four spatial multiplexed OAM modes l_m = ＋ 1,＋2,＋3,＋4 are all close to 10-5,and there is a 2-3 fold increase in the BER performance in comparison with those results without the proposed scheme.In addition,the proposed scheme is more effective for an OAM-multiplexed FSO communication link with a larger OAM mode topological charge interval.The proposed scheme is a promising direction for compensating the interference caused by AT in the OAM-multiplexed FSO communication link.
文摘This paper expounds the necessity of constructing China's rural multiplex financial system as follows.First,overcome the bottleneck effect arising from the shortage of capital in the process of development of rural small and medium-sized enterprise,and increase farmers' income;second,provide capital support for rural modern industrial development,and promote rural economic development;third,rationally allocate capital,master the money flow and coordinate urban-rural development.The paper also profoundly analyzes the difficulties of constructing China's rural multiplex financial system as follows.First,the existing rural financial system structure is irrational and the function is unsound;second,non-governmental financial market is disorderly and the organization is unsound;third,the rural financial system is simple and the overhaul of rural credit cooperatives system has not yet been done;fourth,the construction of rural system of financial laws and regulations is laggard,and the construction of rural multiplex financial system lacks good social environment.The specific thoughts,measures and countermeasures of constructing mechanism innovation of China's rural multiplex financial system are put forward as follows.First,increasingly perfect legal system and provide legal support for constructing China's rural multiplex financial system;second,quicken the overhaul of rural credit cooperatives system and perfect its function in China's rural multiplex financial system;third,strengthen the degree of support and development,and change the situation of simple structure of rural financial system;fourth,reinforce administrative supervision and perfect public services,and standardize the rural financial market order.
基金supported by the National Natural Science Foundation of China(32001532 and 31860411)the National Key Research and Development Program of China,(2022YFF1000020)+1 种基金Hunan Seed Industry Innovation Project(2021NK1012)the Yunnan Tobacco Company Project(2020530000241009)。
文摘The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.
文摘In recent years, the booming band- width demands of dedicated mobile services have driven the rapid development of optical transport networks （OTNs）. Through the in- novative use of emerging coherent optical communication technology and the advance- ment of microelectronics technology, the new-generation 100Gb/s transport technology offers a high line rate and unprecedented re- silience to optical transmission impairments. This paper overviews the bandwidth demands of China Mobile driven by the upcoming roll- out of Time Division-Long Term Evolution （TD-LTE） and presents the 100Gb/s trials at China Mobile that were used to verify the performance of a lOOGb/s system. China Mo- bile＇s considerations, which were based on the trial results, regarding the deployment of lOOGb/s transport systems are introduced, and the requirements of China Mobile for the evo- lution of lOOGb/s transport systems are sum- marized.
文摘In order to rapidly diagnose and differentiate tuberculosis from other bacterial infections, a 16S rRNA gene (16s rDNA)-directed multiplex PCR system was developed. In this system, a pair of universal primers and a tubercle bacillus (Tb)-specific primer were designed based on highly con- served regions and Tb species-specific variable region of bacterial 16s rDNA. A 360bp fragment was detected in all bacteria tested, and a 210bp fragment was found only in Tb. 19 species of known bac- teria including Tb were used for evaluating specificity, universality and sensitivity of the PCR. Candi- da albicans and human diploid cell served as controls. It was found that both 210bp and 360bp frag- ments were amplified only in Tb, and only 360 bp fragment was detected in other 18 species of gener- al bacteria. Candida albicans and human cells were negative for both 360bp and 2l0bp fragments. The lowest detectable level of the PCR was 10 fg of DNA for Escherichia coli and 100 fg of DNA for Tb. The results indicated that this multiplex PCR system for the simultaneous detection of Tb and other common bacteria had higher specificity and sensitivity, as well as good universality and might be useful to rapidly diagnose bacterial infections and effectively distinguish tuberculosis from other bacterial involvement.
文摘Quadrature Spatial Modulation (QSM) is a high spectral efficiency Multiple-Input Multiple Output (MIMO) technique used to improve the spectral efficiency of wireless communication systems. The main concept of QSM is to extend the spatial constellation of the conventional Spatial Modulation (SM) in both the in-phase and quadrature components of the data symbol. In this paper, because QSM-based on Interleaxdng Division Multiplexing (IDM) has not been introduced in the literature as a multiple antenna system, we introduced a novel scheme, called QSM system based on Interleaving Division Multiplexing (QSM-IDM). The antenna sets are also applied to a spreader, before being used to assign an antenna number for information transmission. Analysis and simulations for a flat fading channel show that the proposed QSM-IDM method significantly outperforms the original QSM system with the same data rate, while maintaining a relatively acceptable complexity. The obtained simulation results show that the conducted analysis yields significant improvements for the accuracy of the proposed scheme, with satisfactory complexity.
文摘This paper begins with Nyquist wavelengthdivision multiplexing （WDM） and then introduces fasterthanNyquist. In fasterthanNyquist a certain amount of intersymbol interference （ISI） is accepted, which violates the fundamental principle of Nyquist WDM. This results in muchrelaxed transceiver bandwidth and simpler spectral design. However, in fasterthanNyquist, implementation complexity is shifted from the transmitter side to the receiver side. Therefore, successful application of fasterthanNyquist depends on innovation in the receiver structure. In this paper, we discuss the guidelines for implementing suboptimum, lowcomplexity receivers based on fasterthanNyquist. We suggest that duobinary shaping is a good technique for trading off achievable spectral efficiency, detection performance, and implementation complexity and might be preferable to Nyquist WDM. Experiments are conducted to verify robustness of the proposed technique.
基金supported by the National Natural Science Foundation of China(No.61262075)the Guangxi College Funded Project(No.201201ZD012)
文摘In this paper, an experimental scheme for cordwood color division multiplexing(CDM) visible light communication(VLC) system is proposed. The principle and structure of the experimental scheme are described. Right angle prisms and band-pass filter are used to make the unit of the optical collector and splitter. We can add or subtract the unit as we need. The cordwood CDM-VLC system can also be effectively used as a model to accomplish color shift keying and color division duplexing. The experimental system provides a new way for researching VLC.
文摘The performance of the wavelength division multiplexing (WDM) photonic analogue-to-digital converter (ADC) used for digitization of high-resolution radar systems is evaluated numerically by using the peak signal-to-noise ratio (SNR) metric. Two different WDM photonic ADC architectures are considered for the digitization of radar signals with 5 GHz of bandwidth (spatial resolution of 3 cm), in order to provide a comprehensive study of the compromises present when deploying radar signals with high-resolution: 1) a four-channel architecture with each channel employing an ADC with 5 GSamples/s, and 2) an eight-channel architecture with each channel employing an ADC with 2.5 GSamples/s. For peak powers of the pulsed source between 10 and 20 dBm and a distance between the radar antenna and the sensing object of 2.4 meters, peak SNR levels between 29 and 39 dB are achieved with the eight-channel architecture, which shows higher peak SNR levels when compared with the four-channel architecture. For the eight-channel architecture and for the same peak powers of the pulsed source, peak SNR levels between 11 and 16 dB are obtained when the distance increases to 13.5 meters. With this evaluation using the peak SNR, it is possible to assess the performance limits when choosing a specific radar range, while keeping the same resolution.
基金supported by the National 863 Program under Grant No 2007AA03Z415.
文摘This paper presents the fundamental technique of phase generated carrier （PGC） and its realization on compact reconfigurable input and output （RIO） which adopts real-time and field programmable grate array （FPGA） techniques. Improvement of the PGC technique is also introduced by using peak-to-peak value detection method to reduce the influence of variation of the light intensity. A four-element fibre Bragg gratings （FBG） laser sensor system is conducted in the experiment and the demodulated results demonstrate correlation coefficient as high as 0.995 with the reference signal and the dynamic range to be 120dB@63Hz.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFB0402504)the National Natural Science Foundation of China(Grant Nos.61875069 and 61575076)+1 种基金Hong Kong Scholars Program,China(Grant No.XJ2016026)the Science and Technology Development Plan of Jilin Province,China(Grant Nos.20190302010GX and 20160520091JH)
文摘We propose a novel and efficient multi-functional optical fiber sensor system based on a dense wavelength division multiplexer(DWDM).This system consists of an optical fiber temperature sensor, an optical fiber strain sensor, and a 48-channel DWDM.This system can monitor temperature and strain changes at the same time.The ranges of these two sensors are from-20℃ to 100℃ and from-1000 με to 2000 με, respectively.The sensitivities of the temperature sensor and strain sensor are 0.03572 nm/℃ and 0.03808 nm/N, respectively.With the aid of a broadband source and spectrometer,different kinds and ranges of parameters in the environment can be monitored by using suitable sensors.
基金Project supported by the Key Projects in the Guangzhou Science & Technology Pillar Program of China(Grant No.2008Z1-D501)the Guangdong Key Technologies Research & Development Program of China(Grant No.2007B010400009)+1 种基金the Guangdong Polytechnic Institute Scientific Research Fund,China(Grant No.0901)the Key Laboratory Program of Quantum Information of Chinese Academy of Sciences
文摘Three clock synchronization schemes for a quantum key distribution system are compared experimentally through the outdoor fibre and the interaction physical model of the the clock signal and the the quantum signal in the quantum key distribution system is analysed to propose a new synchronization scheme based on time division multiplexing and wavelength division multiplexing technology to reduce quantum bits error rates under some transmission rate conditions, The proposed synchronization scheme can not only completely eliminate noise photons from the bright background light of the the clock signal, but also suppress the fibre nonlinear crosstalk.
文摘Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (FHCM).A microarray was fabricated to screen mutations in exons 3,5,7,and 8 in cTnI gene.Primers were designed for the PCR (polymerase chain reaction) to amplify the target DNA fragments from fresh blood samples.In order to simplify the PCR process,multiplex PCR technology was investigated in detail.The concentration of Mg^(2+) played an important role in multiplex PCR process,a properly low concentration of Mg^(2+) submitted a better speciality of PCR products.The speciality was also favored when the annealing temperature was reasonably enhanced and 64℃is the optimal annealing temperature for the multiplex PCR systems.When applying the fabricated gene-chip to detect the target fragments from PCR mixture,the signal intensity sequence is in accordance with that from theoretic estimate.
基金supported by the Natural Science Foundation of Hebei Province (No.F2008000116)
文摘An electronic digital equalizer for polarization multiplex coherent fiber optic communication systems is designed to compensate polarization mode dispersion (PMD) and residual chromatic dispersion (CD) of transmission channel. The proposed equalizer is realized with fraction spaced infinite impulse response (IIR) butterfly structure with 21 feedforward taps and 2 feedback taps. Compared with finite impulse response (FIR) structure, this structure can reduce implementation complexity of hardware under the same condition. To keep track of the random variation of channel characteristics, the filter weights are updated by least mean square (LMS) algorithm. The simulation results show that the proposed equalizer can compensate residual chromatic dispersion (CD) of 1600 ps/nm and differential group delay (DGD) of 90 ps simultaneously, and also can increase the PMD and residual CD tolerance of the whole communication system.
