The study aimed to evaluate the diagnosis between SARS-COV-2 and influenza viruses using the Multiplex qPCR molecular method,highlighting the importance of these methods in disease management.In this study,primers and...The study aimed to evaluate the diagnosis between SARS-COV-2 and influenza viruses using the Multiplex qPCR molecular method,highlighting the importance of these methods in disease management.In this study,primers and probes were designed for the hemagglutinin region(HA)of influenza A,the M region of influenza B virus,and the RdRp region of SARS-COV-2.Optimization was performed using qPCR methods,and the method's analytical sensitivity and specificity were assessed.Finally,the process was compared to the commercial kit,Generi-Biotech Company(GB SARS-CoV-2 Influenza A/B).The best annealing temperature for this method was determined to be 58℃.Analytical sensitivity showed detection limits of 500 copies of the virus genome for SARS-CoV-2,250 copies for influenza A,and 500 copies for influenza B.Clinical evaluations confirmed that the designed kit exhibited 100%sensitivity and specificity,identical to the GB commercial kit,establishing its comparable diagnostic performance.展开更多
文摘The study aimed to evaluate the diagnosis between SARS-COV-2 and influenza viruses using the Multiplex qPCR molecular method,highlighting the importance of these methods in disease management.In this study,primers and probes were designed for the hemagglutinin region(HA)of influenza A,the M region of influenza B virus,and the RdRp region of SARS-COV-2.Optimization was performed using qPCR methods,and the method's analytical sensitivity and specificity were assessed.Finally,the process was compared to the commercial kit,Generi-Biotech Company(GB SARS-CoV-2 Influenza A/B).The best annealing temperature for this method was determined to be 58℃.Analytical sensitivity showed detection limits of 500 copies of the virus genome for SARS-CoV-2,250 copies for influenza A,and 500 copies for influenza B.Clinical evaluations confirmed that the designed kit exhibited 100%sensitivity and specificity,identical to the GB commercial kit,establishing its comparable diagnostic performance.
