Introduction: Arbovirus diseases such as dengue and chikungunya threaten public health worldwide. Early and rapid diagnosis and surveillance of dengue virus (DENV) and chikungunya virus (CHIKV) infections are essentia...Introduction: Arbovirus diseases such as dengue and chikungunya threaten public health worldwide. Early and rapid diagnosis and surveillance of dengue virus (DENV) and chikungunya virus (CHIKV) infections are essential to the control of these diseases. In this study, we evaluate the diagnostic performance of our new in-house multiplex RT-qPCR method for detecting DENV serotypes and CHIKV in an external laboratory. Methodology: The evaluation study was conducted on 200 clinical samples of suspected patients for arbovirus disease infection, collected in Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso. Our new multiplex RT-qPCR was compared to the commercial kit, the Zika, Dengue, and Chikungunya (ZDC) Real-Time PCR Assays kit (Bio-Rad, California, USA). Results and Conclusions: Among 200 samples, 21.5% (43/200) were DENV-positive by multiplex RT-qPCR, and 21.5% (43/200) were also DENV-positive by reference real-time RT-PCR. 157 (78.5%) samples tested negative for DENV by both tests (new mRT-qPCR and reference test). The sensitivity and specificity of mRT-qPCR were 100%. The DENV serotypes detected were DENV-1 60.5% (26/43) and DENV-3 39.5% (17/43). CHIKV was not detected in this study. Our new mRT-qPCR is sensitive, cost-effective, simple, and can be used in developing country laboratories.展开更多
Advancements in mode-division multiplexing(MDM)techniques,aimed at surpassing the Shannon limit and augmenting transmission capacity,have garnered significant attention in optical fiber communica-tion,propelling the d...Advancements in mode-division multiplexing(MDM)techniques,aimed at surpassing the Shannon limit and augmenting transmission capacity,have garnered significant attention in optical fiber communica-tion,propelling the demand for high-quality multiplexers and demultiplexers.However,the criteria for ideal-mode multiplexers/demultiplexers,such as performance,scalability,compatibility,and ultra-compactness,have only partially been achieved using conventional bulky devices(e.g.,waveguides,grat-ings,and free space optics)—an issue that will substantially restrict the application of MDM techniques.Here,we present a neuro-meta-router(NMR)optimized through deep learning that achieves spatial multi-mode division and supports multi-channel communication,potentially offering scalability,com-patibility,and ultra-compactness.An MDM communication system based on an NMR is theoretically designed and experimentally demonstrated to enable simultaneous and independent multi-dataset transmission,showcasing a capacity of up to 100 gigabits per second(Gbps)and a symbol error rate down to the order of 104,all achieved without any compensation technologies or correlation devices.Our work presents a paradigm that merges metasurfaces,fiber communications,and deep learning,with potential applications in intelligent metasurface-aided optical interconnection,as well as all-optical pat-tern recognition and classification.展开更多
Recent advancements in artificial intelligence have transformed three-dimensional(3D)optical imaging and metrology,enabling high-resolution and high-precision 3D surface geometry measurements from one single fringe pa...Recent advancements in artificial intelligence have transformed three-dimensional(3D)optical imaging and metrology,enabling high-resolution and high-precision 3D surface geometry measurements from one single fringe pattern projection.However,the imaging speed of conventional fringe projection profilometry(FPP)remains limited by the native sensor refresh rates due to the inherent"one-to-one"synchronization mechanism between pattern projection and image acquisition in standard structured light techniques.Here,we present dual-frequency angular-multiplexed fringe projection profilometry(DFAMFPP),a deep learning-enabled 3D imaging technique that achieves high-speed,high-precision,and large-depth-range absolute 3D surface measurements at speeds 16 times faster than the sensor's native frame rate.By encoding multi-timeframe 3D information into a single multiplexed image using multiple pairs of dual-frequency fringes,high-accuracy absolute phase maps are reconstructed using specially trained two-stage number-theoretical-based deep neural networks.We validate the effectiveness of DFAMFPP through dynamic scene measurements,achieving 10,000 Hz 3D imaging of a running turbofan engine prototype with only a 625 Hz camera.By overcoming the sensor hardware bottleneck,DFAMFPP significantly advances high-speed and ultra-high-speed 3D imaging,opening new avenues for exploring dynamic processes across diverse scientific disciplines.展开更多
Adventitious agents,comprising unintentionally introduced microorganisms in the production of biological products,pose a significant challenge in ensuring the safety of gene therapy products.The revised International ...Adventitious agents,comprising unintentionally introduced microorganisms in the production of biological products,pose a significant challenge in ensuring the safety of gene therapy products.The revised International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH)guildline Q5A(R2)from September 2022 highlights the inclusion of viral vector-based gene therapy products in safety discussions,emphasizing controls in material sourcing,testing,and viral clearance[1].Detecting adventitious virus contamination is complex due to the unique characteristics of gene therapy products and the limitations of routine testing methods.The US Food and Drug Administration(FDA)recommends incorporating routine and specific virus detection methods,including those outlined in various pharmacopeias.Existing control methods have limitations,prompting the need for highly sensitive and broad-spectrum detection approaches.Unlike traditional biological products,gene therapy products primarily consist of live viruses,necessitating methods that distinguish between the main virus and adventitious viruses.Current virus detection techniques,such as polymerase chain reaction(PCR),sequencing,mass spectrometry,and DNA microarrays[2e4],have their drawbacks.展开更多
Multiple quantum well(MQW) Ⅲ-nitride diodes that can simultaneously emit and detect light feature an overlapping region between their electroluminescence and responsivity spectra, which allows them to be simultaneous...Multiple quantum well(MQW) Ⅲ-nitride diodes that can simultaneously emit and detect light feature an overlapping region between their electroluminescence and responsivity spectra, which allows them to be simultaneously used as both a transmitter and a receiver in a wireless light communication system. Here, we demonstrate a mobile light communication system using a time-division multiplexing(TDM) scheme to achieve bidirectional data transmission via the same optical channel.Two identical blue MQW diodes are defined by software as a transmitter or a receiver. To address the light alignment issue, an image identification module integrated with a gimbal stabilizer is used to automatically detect the locations of moving targets;thus, underwater audio communication is realized via a mobile blue-light TDM communication mode. This approach not only uses a single link but also integrates mobile nodes in a practical network.展开更多
Lanthanide(Ln^(3+))-doped luminescent nanocrystals(NCs)with excitation and emission in the second near-infrared biological window(NIRII,1000-1700 nm)have attracted considerable attention in the fields of deep-tissue b...Lanthanide(Ln^(3+))-doped luminescent nanocrystals(NCs)with excitation and emission in the second near-infrared biological window(NIRII,1000-1700 nm)have attracted considerable attention in the fields of deep-tissue bioimaging and non-invasive biodetection,owing to their superior advantages including good photochemical stability,sharp emission peaks,large penetration depth,and high signal-to-noise ratio[1].Conventionally,Yb3t-and Nd3t-sensitized NCs have been utilized as NIR-II luminescent nanoprobes for in vivo bioimaging upon excitation with 980 and 808 nm diode laser,respectively[2].展开更多
Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low inse...Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low insertion loss and modal crosstalk are the key components.In this paper,a low-modal-crosstalk 4-mode MMUX/MDEMUX for the weakly-coupled triple-ring-core few-mode fiber(TRC-FMF)is designed and fabricated with side-polishing processing.The measurement results show that a pair of MMUX/MDEMUX and 25 km weakly-coupled TRC-FMF MDM link achieve low modal crosstalk of lower than−17.5 dB and insertion loss of lower than 11.56 dB for all the four modes.Based on the TRC-FMF and all-fiber MMUX/MDEMUX,an experiment for 25 km real-time 4-mode 3-λwavelength division multiplexing(WDM)-MDM transmission is conducted using commercial 400G optical transport network(OTN)transceivers.The experimental results prove weakly-coupled MDM techniques facilitate a smooth upgrade of the optical transmission system.展开更多
Herein,an attention-grabbing and up-to-date review related to major multiplexing techniques is presented which in-cludes wavelength division multiplexing(WDM),polarization division multiplexing(PDM),space division mul...Herein,an attention-grabbing and up-to-date review related to major multiplexing techniques is presented which in-cludes wavelength division multiplexing(WDM),polarization division multiplexing(PDM),space division multiplexing(SDM),mode division multiplexing(MDM)and orbital angular momentum multiplexing(OAMM).Multiplexing is a mech-anism by which multiple signals are combined into a shared channel used to showcase the maximum capacity of the op-tical links.However,it is critical to develop hybrid multiplexing methods to allow enhanced channel numbers.In this re-view,we have also included hybrid multiplexing techniques such as WDM-PDM,WDM-MDM and PDM-MDM.It is prob-able to attain N×M channels by utilizing N wavelengths and M guided-modes by simply utilizing hybrid WDM-MDM(de)multiplexers.To the best of our knowledge,this review paper is one of its kind which has highlighted the most prom-inent and recent signs of progress in multiplexing techniques in one place.展开更多
Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by pro...Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.展开更多
The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of t...The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.展开更多
Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of ...Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.展开更多
Orbital angular momentum(OAM),described by an azimuthal phase term expej lθT,has unbound orthogonal states with different topological charges l.Therefore,with the explosive growth of global communication capacity,esp...Orbital angular momentum(OAM),described by an azimuthal phase term expej lθT,has unbound orthogonal states with different topological charges l.Therefore,with the explosive growth of global communication capacity,especially for short-distance optical interconnects,light-carrying OAM has proved its great potential to improve transmission capacity and spectral efficiency in the space-division multiplexing system due to its orthogonality,security,and compatibility with other techniques.Meanwhile,100-m freespace optical interconnects become an alternative solution for the“last mile”problem and provide interbuilding communication.We experimentally demonstrate a 260-m secure optical interconnect using OAM multiplexing and 16-ary quadrature amplitude modulation(16-QAM)signals.We study the beam wandering,power fluctuation,channel cross talk,bit-error-rate performance,and link security.Additionally,we also investigate the link performance for 1-to-9 multicasting at the range of 260 m.Considering that the power distribution may be affected by atmospheric turbulence,we introduce an offline feedback process to make it flexibly controllable.展开更多
Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and struct...Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and structured optical field engineering.The exploration of MDMUXs employing cascaded resonant couplers has garnered significant attention owing to their scalability,exceptional integration capabilities,and the anticipated low insertion loss.In this work,we present the successful realization of high-quality orbital angular momentum MDMUX corresponding to topological charges 0,±1,and±2,achieved through the utilization of cascaded fused-biconical tapered couplers.Notably,the measured insertion losses at 1550 nm exhibit remarkable minimal values:0.31,0.10,and 0.64 dB,respectively.Furthermore,the 80%efficiency bandwidths exceed 106,174,and 174 nm for these respective modes.The MDMUX is composed of precisionmanufactured high-quality mode selective couplers(MSCs).Utilizing a proposed supermode propagation method based on mode composition analysis,we precisely describe the operational characteristics of MSCs.Building upon this comprehensive understanding,we embark on a pioneering analysis elucidating the influence of MSC cascading order on the performance of MDMUXs.Our theoretical investigation substantiates that when constructing MDMUXs,MSCs should adhere to a specific cascading sequence.展开更多
CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive a...CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a population.However,resistance alleles formed by end-joining repair pose a significant obstacle.To address this,we create a homing drive targeting the essential hairy gene in Drosophila melanogaster.Nonfunctional resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of hairy.The drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the population.However,the drive does not reach 100%due to the formation of functional resistance alleles despite using four gRNAs.These alleles have a large deletion but likely utilize an alternate start codon.Thus,revised designs targeting more essential regions of a gene may be necessary to avoid such functional resistance.Replacement of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.展开更多
During public health emergencies,the diffusion of negative information can exacerbate the transmission of adverse emotions,such as fear and anxiety.These emotions can adversely affect immune function and,consequently,...During public health emergencies,the diffusion of negative information can exacerbate the transmission of adverse emotions,such as fear and anxiety.These emotions can adversely affect immune function and,consequently,influence the spread of the epidemic.In this study,we established a coupled model incorporating environmental factors to explore the coevolution dynamic process of information-emotions-epidemic dynamics in activity-driven multiplex networks.In this model,environmental factors refer to the external conditions or pressures that affect the spread of information,emotions,and epidemics.These factors include media coverage,public opinion,and the prevalence of diseases in the neighborhood.These layers are dynamically cross-coupled,where the environmental factors in the information layer are influenced by the emotional layer;the higher the levels of anxious states among neighboring individuals,the greater the likelihood of information diffusion.Although environmental factors in the emotional layer are influenced by both the information and epidemic layers,they come from the factors of global information and the proportion of local infections among surrounding neighbors.Subsequently,we utilized the microscopic Markov chain approach to describe the dynamic processes,thereby obtaining the epidemic threshold.Finally,conclusions are drawn through numerical modeling and analysis.The conclusions suggest that when negative information increases,the probability of the transmission of anxious states across the population increases.The transmission of anxious states increases the final size of the disease and decreases its outbreak threshold.Reducing the impact of environmental factors at both the informational and emotional levels is beneficial for controlling the scale of the spread of the epidemic.Our findings can provide a reference for improving public health awareness and behavioral decision-making,mitigating the adverse impacts of anxious states,and ultimately controlling the spread of epidemics.展开更多
The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and re...The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and reception of signals.Enabling IBFD systems requires a substantial mitigation of a transmitter(Tx)’s strong self-interference(SI)signal into the receiver(Rx)channel.However,current state-ofthe-art approaches to tackle this challenge are inefficient in terms of performance,cost,and complexity,hindering the commercialization of IBFD techniques.In this work,we devise and demonstrate an innovative approach to realize IBFD systems that exhibit superior performance with a low-cost and lesscomplex architecture in an all-passive module.Our scheme is based on meticulously combining polarization-division multiplexing(PDM)with ferromagnetic nonreciprocity to achieve ultra-high isolation between Tx and Rx channels.Such an unprecedented conception has become feasible thanks to a concurrent dual-mode circulator—a new component introduced for the first time—as a key feature of our module,and a dual-mode waveguide that transforms two orthogonally polarized waves into two orthogonal waveguide modes.In addition,we propose a unique passive tunable secondary SI cancellation(SIC)mechanism,which is embedded within the proposed module and boosts the isolation over a relatively broad bandwidth.We report,solely in the analog domain,experimental isolation levels of 50,70,and 80 dB over 340,101,and 33 MHz bandwidth at the center frequency of interest,respectively,with excellent tuning capability.Furthermore,the module is tested in two real IBFD scenarios to assess its performance in connection with Tx-to-Rx leakage and modulation error in the presence of a Tx’s strong interference signal.展开更多
Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed ga...Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed gas sensing system basedon optical frequency comb (OFC) calibrated frequency-modulated continuous-wave (FMCW) tuning nonlinearity. Thesystem can be used for multi-parameter synchronous measurement of gas absorption spectrum and multiplexed opticalpath. Multi-channel parallel detection is realized by combining wavelength division multiplexing (WDM) and frequencydivision multiplexing (FDM) techniques. By introducing nonlinear optical crystals, broadband spectrum detection is simultaneouslyachieved over a bandwidth of hundreds of nanometers. An OFC with ultra-high frequency stability is used asthe frequency calibration source, which guarantees the measurement accuracy. The test samples involve H13C14N, C_(2)H_(2)and Rb vapor cells of varying densities and 5 parallel measurement experiments are designed. The results show that themeasurement accuracies of spectral absorption line and the optical path are 150 MHz and 20 m, respectively. The schemeoffers the advantages of multiplexed, multi-parameter, wide spectrum and high resolution detection, which can realize theidentification of multi-gas components and the high-precision inversion of absorption lines under different environments.The proposed sensor demonstrates great potential in the field of high-resolution absorption spectrum measurement for gassensing applications.展开更多
Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical indicators.Ion sensors based on potentiometric methods are a promising platform for monitoring physiological ions in bio...Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical indicators.Ion sensors based on potentiometric methods are a promising platform for monitoring physiological ions in biological subjects.Current semi-implantable devices are mainly based on single-parameter detection.Miniaturized semi-implantable electrodes for multiparameter sensing have more restrictions on the electrode size due to biocompatibility considerations,but reducing the electrode surface area could potentially limit electrode sensitivity.This study developed a semi-implantable device system comprising a multiplexed microfilament electrode cluster(MMEC)and a printed circuit board for real-time monitoring of intra-tissue K^(+),Ca^(2+),and Na^(+)concentrations.The electrode surface area was less important for the potentiometric sensing mechanism,suggesting the feasibility of using a tiny fiber-like electrode for potentiometric sensing.The MMEC device exhibited a broad linear response(K^(+):2–32 mmol/L;Ca^(2+):0.5–4 mmol/L;Na^(+):10–160 mmol/L),high sensitivity(about 20–45 mV/decade),temporal stability(>2weeks),and good selectivity(>80%)for the above ions.In vitro detection and in vivo subcutaneous and brain experiment results showed that the MMEC system exhibits good multi-ion monitoring performance in several complex environments.This work provides a platform for the continuous real-time monitoring of ion fluctuations in different situations and has implications for developing smart sensors to monitor human health.展开更多
BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management.Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevent...BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management.Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevention.Quantum dot-encoded microspheres have been widely used in immunodetection.The integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid analysis.Thus,establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease diagnosis.AIM To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology,which forms the foundation for the assays of multiple respiratory virus biomarkers.METHODS Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and B.The known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer,and the detection conditions were optimized to establish the influenza A and B antigen codetection method,which was utilized for their detection in clinical samples.The results were compared with the fluorescence quantitative polymerase chain reaction(PCR)method to validate the clinical performance of this method.RESULTS The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens,respectively,which both ranged from 15.6 to 250000 pg/mL.In the clinical sample evaluation,the proposed method well correlated with the fluorescent quantitative PCR method,with positive,negative,and overall compliance rates of 57.4%,100%,and 71.6%,respectively.CONCLUSION A multiplex assay for quantitative detection of influenza A and B virus antigens has been established,which is characterized by high sensitivity,good specificity,and a wide detection range and is promising for clinical applications.展开更多
The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA)....The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.展开更多
文摘Introduction: Arbovirus diseases such as dengue and chikungunya threaten public health worldwide. Early and rapid diagnosis and surveillance of dengue virus (DENV) and chikungunya virus (CHIKV) infections are essential to the control of these diseases. In this study, we evaluate the diagnostic performance of our new in-house multiplex RT-qPCR method for detecting DENV serotypes and CHIKV in an external laboratory. Methodology: The evaluation study was conducted on 200 clinical samples of suspected patients for arbovirus disease infection, collected in Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso. Our new multiplex RT-qPCR was compared to the commercial kit, the Zika, Dengue, and Chikungunya (ZDC) Real-Time PCR Assays kit (Bio-Rad, California, USA). Results and Conclusions: Among 200 samples, 21.5% (43/200) were DENV-positive by multiplex RT-qPCR, and 21.5% (43/200) were also DENV-positive by reference real-time RT-PCR. 157 (78.5%) samples tested negative for DENV by both tests (new mRT-qPCR and reference test). The sensitivity and specificity of mRT-qPCR were 100%. The DENV serotypes detected were DENV-1 60.5% (26/43) and DENV-3 39.5% (17/43). CHIKV was not detected in this study. Our new mRT-qPCR is sensitive, cost-effective, simple, and can be used in developing country laboratories.
基金supported by the National Key Research and Development Program of China(2023YFB2804704)the National Natural Science Foundation of China(12174292,12374278,and 62105250).
文摘Advancements in mode-division multiplexing(MDM)techniques,aimed at surpassing the Shannon limit and augmenting transmission capacity,have garnered significant attention in optical fiber communica-tion,propelling the demand for high-quality multiplexers and demultiplexers.However,the criteria for ideal-mode multiplexers/demultiplexers,such as performance,scalability,compatibility,and ultra-compactness,have only partially been achieved using conventional bulky devices(e.g.,waveguides,grat-ings,and free space optics)—an issue that will substantially restrict the application of MDM techniques.Here,we present a neuro-meta-router(NMR)optimized through deep learning that achieves spatial multi-mode division and supports multi-channel communication,potentially offering scalability,com-patibility,and ultra-compactness.An MDM communication system based on an NMR is theoretically designed and experimentally demonstrated to enable simultaneous and independent multi-dataset transmission,showcasing a capacity of up to 100 gigabits per second(Gbps)and a symbol error rate down to the order of 104,all achieved without any compensation technologies or correlation devices.Our work presents a paradigm that merges metasurfaces,fiber communications,and deep learning,with potential applications in intelligent metasurface-aided optical interconnection,as well as all-optical pat-tern recognition and classification.
基金supported by National Key Research and Development Program of China(2022YFB2804603,2022YFB2804605)National Natural Science Foundation of China(U21B2033)+4 种基金Fundamental Research Funds forthe Central Universities(2023102001,2024202002)National Key Laborato-ry of Shock Wave and Detonation Physics(JCKYS2024212111)China Post-doctoral Science Fund(2023T160318)Open Research Fund of JiangsuKey Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX25_0695,SJCX25_0188)。
文摘Recent advancements in artificial intelligence have transformed three-dimensional(3D)optical imaging and metrology,enabling high-resolution and high-precision 3D surface geometry measurements from one single fringe pattern projection.However,the imaging speed of conventional fringe projection profilometry(FPP)remains limited by the native sensor refresh rates due to the inherent"one-to-one"synchronization mechanism between pattern projection and image acquisition in standard structured light techniques.Here,we present dual-frequency angular-multiplexed fringe projection profilometry(DFAMFPP),a deep learning-enabled 3D imaging technique that achieves high-speed,high-precision,and large-depth-range absolute 3D surface measurements at speeds 16 times faster than the sensor's native frame rate.By encoding multi-timeframe 3D information into a single multiplexed image using multiple pairs of dual-frequency fringes,high-accuracy absolute phase maps are reconstructed using specially trained two-stage number-theoretical-based deep neural networks.We validate the effectiveness of DFAMFPP through dynamic scene measurements,achieving 10,000 Hz 3D imaging of a running turbofan engine prototype with only a 625 Hz camera.By overcoming the sensor hardware bottleneck,DFAMFPP significantly advances high-speed and ultra-high-speed 3D imaging,opening new avenues for exploring dynamic processes across diverse scientific disciplines.
基金financially supported by Beijing Municipal Science&Technology Commission,China(Grant No.:Z221100007922015)Youth Development Research Foundation of National Institutes for Food and Drug Control,China(Grant No.:2020B1).
文摘Adventitious agents,comprising unintentionally introduced microorganisms in the production of biological products,pose a significant challenge in ensuring the safety of gene therapy products.The revised International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH)guildline Q5A(R2)from September 2022 highlights the inclusion of viral vector-based gene therapy products in safety discussions,emphasizing controls in material sourcing,testing,and viral clearance[1].Detecting adventitious virus contamination is complex due to the unique characteristics of gene therapy products and the limitations of routine testing methods.The US Food and Drug Administration(FDA)recommends incorporating routine and specific virus detection methods,including those outlined in various pharmacopeias.Existing control methods have limitations,prompting the need for highly sensitive and broad-spectrum detection approaches.Unlike traditional biological products,gene therapy products primarily consist of live viruses,necessitating methods that distinguish between the main virus and adventitious viruses.Current virus detection techniques,such as polymerase chain reaction(PCR),sequencing,mass spectrometry,and DNA microarrays[2e4],have their drawbacks.
基金jointly supported by the National Natural Science Foundation of China (U21A20495)Natural Science Foundation of Jiangsu Province (BG2024023)+1 种基金National Key Research and Development Program of China (2022YFE0112000)111 Project (D17018)。
文摘Multiple quantum well(MQW) Ⅲ-nitride diodes that can simultaneously emit and detect light feature an overlapping region between their electroluminescence and responsivity spectra, which allows them to be simultaneously used as both a transmitter and a receiver in a wireless light communication system. Here, we demonstrate a mobile light communication system using a time-division multiplexing(TDM) scheme to achieve bidirectional data transmission via the same optical channel.Two identical blue MQW diodes are defined by software as a transmitter or a receiver. To address the light alignment issue, an image identification module integrated with a gimbal stabilizer is used to automatically detect the locations of moving targets;thus, underwater audio communication is realized via a mobile blue-light TDM communication mode. This approach not only uses a single link but also integrates mobile nodes in a practical network.
基金supported by the National Natural Science Foundation of China(Nos.12474418,U22A20398,and 22135008).
文摘Lanthanide(Ln^(3+))-doped luminescent nanocrystals(NCs)with excitation and emission in the second near-infrared biological window(NIRII,1000-1700 nm)have attracted considerable attention in the fields of deep-tissue bioimaging and non-invasive biodetection,owing to their superior advantages including good photochemical stability,sharp emission peaks,large penetration depth,and high signal-to-noise ratio[1].Conventionally,Yb3t-and Nd3t-sensitized NCs have been utilized as NIR-II luminescent nanoprobes for in vivo bioimaging upon excitation with 980 and 808 nm diode laser,respectively[2].
基金supported in part by the ZTE Industry-University-Institute Cooperation Funds.
文摘Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low insertion loss and modal crosstalk are the key components.In this paper,a low-modal-crosstalk 4-mode MMUX/MDEMUX for the weakly-coupled triple-ring-core few-mode fiber(TRC-FMF)is designed and fabricated with side-polishing processing.The measurement results show that a pair of MMUX/MDEMUX and 25 km weakly-coupled TRC-FMF MDM link achieve low modal crosstalk of lower than−17.5 dB and insertion loss of lower than 11.56 dB for all the four modes.Based on the TRC-FMF and all-fiber MMUX/MDEMUX,an experiment for 25 km real-time 4-mode 3-λwavelength division multiplexing(WDM)-MDM transmission is conducted using commercial 400G optical transport network(OTN)transceivers.The experimental results prove weakly-coupled MDM techniques facilitate a smooth upgrade of the optical transmission system.
基金financially supported by the Russian Foundation for Basic Research(grant No.18-29-20045)for WDM,MDM and hybrid WDM-MDM,WDM-PDM sectionsthe Russian Science Foundation(grant No.21-79-20075)for PDM,OAMM and hybrid PDM-MDM sectionsthe Ministry of Science and Higher Education of the Russian Federation under the FSRC"Crystallography and Photonics"of the Russian Academy of Sciences(the state task No.007-GZ/Ch3363/26)for comparative analysis.
文摘Herein,an attention-grabbing and up-to-date review related to major multiplexing techniques is presented which in-cludes wavelength division multiplexing(WDM),polarization division multiplexing(PDM),space division multiplexing(SDM),mode division multiplexing(MDM)and orbital angular momentum multiplexing(OAMM).Multiplexing is a mech-anism by which multiple signals are combined into a shared channel used to showcase the maximum capacity of the op-tical links.However,it is critical to develop hybrid multiplexing methods to allow enhanced channel numbers.In this re-view,we have also included hybrid multiplexing techniques such as WDM-PDM,WDM-MDM and PDM-MDM.It is prob-able to attain N×M channels by utilizing N wavelengths and M guided-modes by simply utilizing hybrid WDM-MDM(de)multiplexers.To the best of our knowledge,this review paper is one of its kind which has highlighted the most prom-inent and recent signs of progress in multiplexing techniques in one place.
基金supported financially by“Kunlun Talents High-end Innovation and Entrepreneurship Talents”of Qinghai Province in 2022National Natural Science Foundation of China(Nos.22322401 and 82073816)Beijing Nova Program(No.20220484055)。
文摘Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.
基金supported by the National Natural Science Foundation of China(32001532 and 31860411)the National Key Research and Development Program of China,(2022YFF1000020)+1 种基金Hunan Seed Industry Innovation Project(2021NK1012)the Yunnan Tobacco Company Project(2020530000241009)。
文摘The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement.Currently available genome-editing tools have a limited number of targets,restricting their application in genetic research.In this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software package.By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was observed.This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.
文摘Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.
基金supported by the National Natural Science Foundation of China (Grant Nos.62125503,62261160388,and 62101198)the Natural Science Foundation of Hubei Province of China (Grant Nos.2021CFB011 and 2023AFA028)+2 种基金the Key R&D Program of Hubei Province of China (Grant Nos.2020BAB001 and 2021BAA024)Shenzhen Science and Technology Program (Grant No.JCYJ20200109114018750)the Innovation Project of Optics Valley Laboratory (Grant Nos.OVL2021BG004 and OVL2023ZD004).
文摘Orbital angular momentum(OAM),described by an azimuthal phase term expej lθT,has unbound orthogonal states with different topological charges l.Therefore,with the explosive growth of global communication capacity,especially for short-distance optical interconnects,light-carrying OAM has proved its great potential to improve transmission capacity and spectral efficiency in the space-division multiplexing system due to its orthogonality,security,and compatibility with other techniques.Meanwhile,100-m freespace optical interconnects become an alternative solution for the“last mile”problem and provide interbuilding communication.We experimentally demonstrate a 260-m secure optical interconnect using OAM multiplexing and 16-ary quadrature amplitude modulation(16-QAM)signals.We study the beam wandering,power fluctuation,channel cross talk,bit-error-rate performance,and link security.Additionally,we also investigate the link performance for 1-to-9 multicasting at the range of 260 m.Considering that the power distribution may be affected by atmospheric turbulence,we introduce an offline feedback process to make it flexibly controllable.
基金supported by the National Key Research and Development Program of China (Grant No.2018YFB1801802)the National Natural Science Foundation of China (Grant Nos.62375143 and 61835006).
文摘Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and structured optical field engineering.The exploration of MDMUXs employing cascaded resonant couplers has garnered significant attention owing to their scalability,exceptional integration capabilities,and the anticipated low insertion loss.In this work,we present the successful realization of high-quality orbital angular momentum MDMUX corresponding to topological charges 0,±1,and±2,achieved through the utilization of cascaded fused-biconical tapered couplers.Notably,the measured insertion losses at 1550 nm exhibit remarkable minimal values:0.31,0.10,and 0.64 dB,respectively.Furthermore,the 80%efficiency bandwidths exceed 106,174,and 174 nm for these respective modes.The MDMUX is composed of precisionmanufactured high-quality mode selective couplers(MSCs).Utilizing a proposed supermode propagation method based on mode composition analysis,we precisely describe the operational characteristics of MSCs.Building upon this comprehensive understanding,we embark on a pioneering analysis elucidating the influence of MSC cascading order on the performance of MDMUXs.Our theoretical investigation substantiates that when constructing MDMUXs,MSCs should adhere to a specific cascading sequence.
基金supported by laboratory startup funds from Peking University and the Center for Life Sciences,as well as the grants from the National Science Foundation of China(32302455 and 32270672)。
文摘CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant insects.They operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a population.However,resistance alleles formed by end-joining repair pose a significant obstacle.To address this,we create a homing drive targeting the essential hairy gene in Drosophila melanogaster.Nonfunctional resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of hairy.The drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the population.However,the drive does not reach 100%due to the formation of functional resistance alleles despite using four gRNAs.These alleles have a large deletion but likely utilize an alternate start codon.Thus,revised designs targeting more essential regions of a gene may be necessary to avoid such functional resistance.Replacement of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.
基金partially supported by the National Natural Science Foundation of China(Grant No.72174121)the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learningthe Natural Science Foundation of Shanghai(Grant No.21ZR1444100)。
文摘During public health emergencies,the diffusion of negative information can exacerbate the transmission of adverse emotions,such as fear and anxiety.These emotions can adversely affect immune function and,consequently,influence the spread of the epidemic.In this study,we established a coupled model incorporating environmental factors to explore the coevolution dynamic process of information-emotions-epidemic dynamics in activity-driven multiplex networks.In this model,environmental factors refer to the external conditions or pressures that affect the spread of information,emotions,and epidemics.These factors include media coverage,public opinion,and the prevalence of diseases in the neighborhood.These layers are dynamically cross-coupled,where the environmental factors in the information layer are influenced by the emotional layer;the higher the levels of anxious states among neighboring individuals,the greater the likelihood of information diffusion.Although environmental factors in the emotional layer are influenced by both the information and epidemic layers,they come from the factors of global information and the proportion of local infections among surrounding neighbors.Subsequently,we utilized the microscopic Markov chain approach to describe the dynamic processes,thereby obtaining the epidemic threshold.Finally,conclusions are drawn through numerical modeling and analysis.The conclusions suggest that when negative information increases,the probability of the transmission of anxious states across the population increases.The transmission of anxious states increases the final size of the disease and decreases its outbreak threshold.Reducing the impact of environmental factors at both the informational and emotional levels is beneficial for controlling the scale of the spread of the epidemic.Our findings can provide a reference for improving public health awareness and behavioral decision-making,mitigating the adverse impacts of anxious states,and ultimately controlling the spread of epidemics.
基金supported by a Natural Sciences and Engineering Research Council(NSERC)-sponsored Industrial Research Chair program,an NSERC Discovery Grantin part by the Fonds de recherche du Québec Nature et technologies(FRQNT)Doctoral Fellowship of Amir Afshani funded by the Government of Québec Province.
文摘The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and reception of signals.Enabling IBFD systems requires a substantial mitigation of a transmitter(Tx)’s strong self-interference(SI)signal into the receiver(Rx)channel.However,current state-ofthe-art approaches to tackle this challenge are inefficient in terms of performance,cost,and complexity,hindering the commercialization of IBFD techniques.In this work,we devise and demonstrate an innovative approach to realize IBFD systems that exhibit superior performance with a low-cost and lesscomplex architecture in an all-passive module.Our scheme is based on meticulously combining polarization-division multiplexing(PDM)with ferromagnetic nonreciprocity to achieve ultra-high isolation between Tx and Rx channels.Such an unprecedented conception has become feasible thanks to a concurrent dual-mode circulator—a new component introduced for the first time—as a key feature of our module,and a dual-mode waveguide that transforms two orthogonally polarized waves into two orthogonal waveguide modes.In addition,we propose a unique passive tunable secondary SI cancellation(SIC)mechanism,which is embedded within the proposed module and boosts the isolation over a relatively broad bandwidth.We report,solely in the analog domain,experimental isolation levels of 50,70,and 80 dB over 340,101,and 33 MHz bandwidth at the center frequency of interest,respectively,with excellent tuning capability.Furthermore,the module is tested in two real IBFD scenarios to assess its performance in connection with Tx-to-Rx leakage and modulation error in the presence of a Tx’s strong interference signal.
基金the National Natural Science Foun-dation of China(Grant No.52375546)the National Key Research and Development Program of China(Grant No.2022YFF0705701).
文摘Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed gas sensing system basedon optical frequency comb (OFC) calibrated frequency-modulated continuous-wave (FMCW) tuning nonlinearity. Thesystem can be used for multi-parameter synchronous measurement of gas absorption spectrum and multiplexed opticalpath. Multi-channel parallel detection is realized by combining wavelength division multiplexing (WDM) and frequencydivision multiplexing (FDM) techniques. By introducing nonlinear optical crystals, broadband spectrum detection is simultaneouslyachieved over a bandwidth of hundreds of nanometers. An OFC with ultra-high frequency stability is used asthe frequency calibration source, which guarantees the measurement accuracy. The test samples involve H13C14N, C_(2)H_(2)and Rb vapor cells of varying densities and 5 parallel measurement experiments are designed. The results show that themeasurement accuracies of spectral absorption line and the optical path are 150 MHz and 20 m, respectively. The schemeoffers the advantages of multiplexed, multi-parameter, wide spectrum and high resolution detection, which can realize theidentification of multi-gas components and the high-precision inversion of absorption lines under different environments.The proposed sensor demonstrates great potential in the field of high-resolution absorption spectrum measurement for gassensing applications.
基金The authors would like to acknowledge financial support from the National Key R&D Program of China(Nos.2021YFF1200700 and 2021YFA0911100)the National Natural Science Foundation of China(Nos.T2225010,32171399,and 32171456)+4 种基金the Fundamental Research Funds for the Central Universities,Sun Yat-Sen University(No.22dfx02)Pazhou Lab,Guangzhou(No.PZL2021KF0003)The authors also would like to thank the funding support from the Opening Project of Key Laboratory of Microelectronic Devices&Integrated Technology,Institute of Microelectronics,Chinese Academy of Sciences,and State Key Laboratory of Precision Measuring Technology and Instruments(No.pilab2211)QQOY would like to thank the China Postdoctoral Science Foundation(No.2022M713645)JL would like to thank the National Natural Science Foundation of China(No.62105380)and the China Postdoctoral Science Foundation(No.2021M693686).
文摘Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical indicators.Ion sensors based on potentiometric methods are a promising platform for monitoring physiological ions in biological subjects.Current semi-implantable devices are mainly based on single-parameter detection.Miniaturized semi-implantable electrodes for multiparameter sensing have more restrictions on the electrode size due to biocompatibility considerations,but reducing the electrode surface area could potentially limit electrode sensitivity.This study developed a semi-implantable device system comprising a multiplexed microfilament electrode cluster(MMEC)and a printed circuit board for real-time monitoring of intra-tissue K^(+),Ca^(2+),and Na^(+)concentrations.The electrode surface area was less important for the potentiometric sensing mechanism,suggesting the feasibility of using a tiny fiber-like electrode for potentiometric sensing.The MMEC device exhibited a broad linear response(K^(+):2–32 mmol/L;Ca^(2+):0.5–4 mmol/L;Na^(+):10–160 mmol/L),high sensitivity(about 20–45 mV/decade),temporal stability(>2weeks),and good selectivity(>80%)for the above ions.In vitro detection and in vivo subcutaneous and brain experiment results showed that the MMEC system exhibits good multi-ion monitoring performance in several complex environments.This work provides a platform for the continuous real-time monitoring of ion fluctuations in different situations and has implications for developing smart sensors to monitor human health.
基金Shenzhen Guangming District Soft Science Research Project,No.2021R01097。
文摘BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease management.Rapid and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak prevention.Quantum dot-encoded microspheres have been widely used in immunodetection.The integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid analysis.Thus,establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease diagnosis.AIM To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology,which forms the foundation for the assays of multiple respiratory virus biomarkers.METHODS Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and B.The known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer,and the detection conditions were optimized to establish the influenza A and B antigen codetection method,which was utilized for their detection in clinical samples.The results were compared with the fluorescence quantitative polymerase chain reaction(PCR)method to validate the clinical performance of this method.RESULTS The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens,respectively,which both ranged from 15.6 to 250000 pg/mL.In the clinical sample evaluation,the proposed method well correlated with the fluorescent quantitative PCR method,with positive,negative,and overall compliance rates of 57.4%,100%,and 71.6%,respectively.CONCLUSION A multiplex assay for quantitative detection of influenza A and B virus antigens has been established,which is characterized by high sensitivity,good specificity,and a wide detection range and is promising for clinical applications.
文摘The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.
